The overall aim of this thesis was to validate diffusive samplers for measurements of nitrogen dioxide (NO2) and nitrogen oxides (NOx). The Willems badge was validated for NO2 measurements both in laboratory tests and in field tests (Paper I-II). The sampling rate was 40.0 mL/min for ambient air concentrations and 46.0 mL/min for higher concentrations. No effects of different factors on sampling rate were found except for a reduced sampling rate at low wind velocity. The results of the laboratory validation were confirmed in field tests in ambient air and with personal sampling. The correlation between diffusive samplers and the reference monitor was good for ambient measurements. In conclusion, the Willems badge performs well at wind velocities down to 0.3 m/s, and this makes it suitable for personal sampling but less suitable for measurements in indoor air where the wind velocity is lower. Paper III reports about the field validation of the Ogawa diffusive samplers. Absolute humidity and temperature were found to have the strongest effect on sampling rate with lower uptake rates at low absolute humidity or temperature. The sampling rates above 0 °C were 8.6 mL/min for NO2 and 9.9 mL/min for NOx. NO2 and NOx concentrations that were determined using the manufacturer’s protocol were either underestimated or overestimated. The agreement between concentrations measured by the Ogawa sampler and the reference monitor was improved when field-determined sampling rates were used to calculate concentrations. Paper IV is based on a study with the aim of assessing the exposure of the Swedish general population to NO2 and some carcinogenic substances. The surveys were performed in one of five Swedish cities every year. In each survey, personal measurements of NO2 and some carcinogenic substances were conducted on 40 randomly selected individuals. In the study presented in this thesis, the NO2 part of the study is in focus and results were available for eight surveys conducted across the five cities. The estimated arithmetic mean concentration for the general Swedish population was 14.1 μg/m3. The exposure level for NO2 was higher for smokers compared with non-smokers, and the NO2 exposure levels were higher for people who had gas stoves at home or who were exposed at their workplace. The exposure was lower for those who had oil heating in their houses.

The aim of this thesis is to analyze and evaluate temporal and spatial patterns of concentrations of ambient ozone in the CHKO Jizerské hory. The Jizerské hory Mts. are an area where one of the highest concentrations of ambient ozone are measured every year and, therefore, our attention was focused on this area. The measurements were carried out between 2006 and 2010 at 13 sites (714-1000 m n. m.) in the CHKO Jizerské hory. From the input data, which were fortnightly average nitrate concentrations created on the filters of Ogawa passive samplers, fortnightly average concentrations of ambient ozone were computed using the empirical flow. Ambient ozone concentrations ranged from 13,8 ppb (year 2007) to 72,1 ppb (year 2006). From the five-year period under study defy the year 2006, when the highest concentrations of ambient ozone were measured. The lowest concentrations occurred in 2009. In all years, the concentrations of ambient ozone increased with increasing elevation and this gradient ranged from 2,7 to 4,6 ppb on 100 meters altitude. The accuracy and precision of measurement was very good (accuracy: R2 = 82 %; precision: R2 = 98 %). By multiple linear regression it was found, that ambient ozone concentration are influenced, in addition to the altitude, by ozone concentrations from previous...

The Copper Creek thrust fault in the southern Appalachians places Cambrian over Ordovician sedimentary strata. The fault accommodated displacement of 15-20 km at 100-180 °C. Along the hanging wall-footwall contact, microstructures within a ~2 cm thick calcite and shale shear zone suggest that calcite, not shale, controlled the rheology of the shear zone rocks. While shale deformed brittley, plasticity-induced fracturing in calcite resulted in ultrafine-grained (<1.0 μm) fault rocks that deformed by grain boundary sliding (GBS) accommodated primarily by diffusion creep, suggesting low flow stresses. Optical and electron microscopy of samples from a transect across the footwall shale into the shear zone, shows the evolution of rheology within the shear zone. Sedimentary laminations 1 cm below the shear zone are cut by minor faults, stylolites, and fault-parallel and perpendicular calcite veins. At vein intersections, calcite grain size is reduced (to ~0.3 μm), and microstructures include inter-and-intragranular fractures, four-grain junctions, and interpenetrating boundaries. Porosity rises to 6 percent from <1 percent in coarse (25 μm) areas of calcite veins. In coarse-grained calcite, trails of voids follow twin boundaries, and voids occur at twin-twin and twin-grain boundary intersections. At the shear zone-footwall contact, a 350 μm thick calcite band contains coarseand ultrafine-grained layers. Ultrafine-grained (~0.34 μm) layers contain microstructures similar to those at vein intersections in the footwall and display no lattice-preferred orientation (LPO). Coarse-grained layers cross-cut grain-boundary alignments in the ultrafine-grained layers; coarse grains display twins and a strong LPO. Within the shear zone, ultrafine-grained calcite-aggregate clasts and shale clasts (5-350 μm) lie within an ultrafine-grained calcite (<0.31 μm) and shale matrix. Ultrafinegrained calcite (<0.31 μm) forms an interconnected network around the matrix shale. Calcite vein microstructures suggest veins continued to form during deformation. Fractures at twin-twin and twin-grain boundary intersections suggest grain size reduction by plasticity-induced fracturing, resulting in <1 μm grains. Interpenetrating boundaries, four-grain junctions, and no LPO indicate the ultrafine-grained calcite deformed by viscous grain boundary sliding. The evolution of the ultrafine-grain shear zone rocks by a combination of plastic and brittle processes and the deformation of the interconnected network of ultrafine-grained calcite by viscous GBS enabled a large displacement along a narrow fault zone.

Rainforests are of great importance to global carbon cycling, but the importance of deforestation and change in land use is poorly understood due to a lack of studies quantifying the difference in carbon fluxes between original rainforest and agricultural land. Furthermore, the aquatic outgassing of neotropical systems have been proven to have greater impact on global carbon cycling than previously anticipated (Richey et al 2002).In this study we investigated the aquatic concentration and daily diffusive gas flux of CO2 and CH4 from 4 pristine sites and 4 impacted sites, respectively, in 4 streams running along a gradient of anthropological impaction in the Atlantic Rainforest, Brazil. Statistically significant differences between pristine and impacted sites were found in all streams for both CO2 and CH4. On average, the impacted sites were found to be emitting almost three times as much C into the atmosphere as the pristine sites, mainly owing to CO2 emissions (14172±5226 mg C m-2 d-1). Exploring an area of the neotropical carbon cycle that is not yet fully understood, the study draws attention to the significant difference in aquatic outgassing from rivers observed at different impaction levels and highlights the need for further field studies.

Maintaining the intrinsic excitability of neurons is crucial for stable brain activity. This can be achieved by the homeostatic regulation of membrane ion channel conductances, although it is not well understood how these processes influence broader aspects of neuron and network function. One of the many mechanisms which contribute towards this task is the modulation of potassium channel conductances by activity-dependent nitric oxide signalling. Here, we first investigate this mechanism in a conductance-based neuron model. By fitting the model to experimental data we find that nitric oxide signalling improves synaptic transmission fidelity at high firing rates, but that there is an increase in the metabolic cost of action potentials associated with this improvement. Although the improvement in function had been observed previously in experiment, the metabolic constraint was unknown. This additional constraint provides a plausible explanation for the selective activation of nitric oxide signalling only at high firing rates. In addition to mediating homeostatic control of intrinsic excitability, nitric oxide can diffuse freely across cell membranes, providing a unique mechanism for neurons to communicate within a network, independent of synaptic connectivity. We next conduct a theoretical investigation of the distinguishing roles of diffusive homeostasis mediated by nitric oxide in comparison with canonical non-diffusive homeostasis in cortical networks. We find that both forms of homeostasis robustly maintain stable activity. However, the resulting networks differ, with diffusive homeostasis maintaining substantial heterogeneity in activity levels of individual neurons, a feature disrupted in networks with non-diffusive homeostasis. This results in networks capable of representing input heterogeneity, and linearly responding over a broader range of inputs than those undergoing non-diffusive homeostasis. We further show that diffusive homeostasis interferes less than non-diffusive homeostasis in the synaptic weight dynamics of networks undergoing Hebbian plasticity. Overall, these results suggest a novel homeostatic mechanism for maintaining stable network activity while simultaneously minimising metabolic cost and conserving network functionality.

La tomographie de fluorescence est une méthode d'imagerie préclinique et clinique permettant de localiser des traceurs fluorescents préalablement injectés ou naturellement présents dans un organisme vivant. Ce travail de thèse à consisté, dans un premier temps, à définir l'architecture et les conditions d'utilisation optimales d'un tomographe de fluorescence continu appliqué à l'observation de tissus de faible épaisseur. On s'est en particulier attaché à traiter le problème de l'observation de tissus hétérogènes et d'organes fortement absorbants. Dans un deuxième temps, pour observer des tissus de plus grande épaisseur, nous avons montré l'apport de la mesure du temps de vol moyen des photons pour améliorer la localisation d'inclusions fluorescentes. Les performances de deux types de systèmes capables de réaliser ce type de mesure ont été comparées et nous avons proposé un protocole permettant d'en optimiser les principaux paramètres. Notre procédé a été appliqué à la problématique du guidage de biopsies prostatiques. Il pourra aussi être utilisé pour visualiser d'autres pathologies moyennant une simple adaptation. / Fluorescence tomography is a preclinical and clinical imaging method which aims to localize fluorescent probes injected into a living organism. In this thesis work, we defined the optimal design and parameters of a continuous wave fluorescent tomograph applied to thin tissue observation. We handled the problem of observing heterogeneous and highly absorbing organs. Secondly, we showed the advantage of time of flight measurement for inclusion detection through thicker tissues. The detection performances of two types of system capable of these measurements were compared and we proposed a protocol to optimize their main parameters. This process was applied to the problematic of prostatic biopsy guiding. It would also be used to detect and localize other pathologies by means of a simple adaptation.

Acousto-optic (AO) sensing and imaging (AOI) is a dual-wave modality that combines ultrasound with diffusive light to measure and/or image the optical properties of optically diffusive media, including biological tissues such as breast and brain. The light passing through a focused ultrasound beam undergoes a phase modulation at the ultrasound frequency that is detected using an adaptive interferometer scheme employing a GaAs photorefractive crystal (PRC). The PRC-based AO system operating at 1064 nm is described, along with the underlying theory, validating experiments, characterization, and optimization of this sensing and imaging apparatus. The spatial resolution of AO sensing, which is determined by spatial dimensions of the ultrasound beam or pulse, can be sub-millimeter for megahertz-frequency sound waves.A modified approach for quantifying the optical properties of diffuse media with AO sensing employs the ratio of AO signals generated at two different ultrasound focal pressures. The resulting “pressure contrast signal” (PCS), once calibrated for a particular set of pressure pulses, yields a direct measure of the spatially averaged optical transport attenuation coefficient within the interaction volume between light and sound. This is a significant improvement over current AO sensing methods since it produces a quantitative measure of the optical properties of optically diffuse media without a priori knowledge of the background illumination. It can also be used to generate images based on spatial variations in both optical scattering and absorption. Finally, the AO sensing system is modified to monitor the irreversible optical changes associated with the tissue heating from high intensity focused ultrasound (HIFU) therapy, providing a powerful method for noninvasively sensing the onset and growth of thermal lesions in soft tissues. A single HIFU transducer is used to simultaneously generate tissue damage and pump the AO interaction. Experimental results performed in excised chicken breast demonstrate that AO sensing can identify the onset and growth of lesion formation in real time and, when used as feedback to guide exposure parameters, results in more predictable lesion formation. / Bernard M. Gordon Center for Subsurface and Imaging Systems (CenSSIS) via the NSF ERC award number EEC-9986821.

No description available.

<p> Matrix diffusion from planar fractures was studied both mathematically and through physical model experiments. A conceptual model was developed based on previous work by Parker (1994) and Crank (1956). Mathematical models were developed to simulate diffusion from 2D and 3D instantaneous disk sources and a 3 D continuous disk source. The models were based on analytical solutions previously developed by Carslaw and Jaeger (1959). Analytical solution is not available for the total mass diffused into the porous matrix for a 3D continuous disk source, and it was therefore calculated through the summation of the iso-concentration lines, which were assumed to be a semi-spherical shape.</p> <p> The mathematical simulations indicated that the 2D scenario produces significantly different results from the 3D scenario, the time for mass disappearance is significantly larger for continuous sources than for instantaneous sources, the normalized concentration generally decreased over time for instantaneous sources while it increased over time for continuous sources, diffusion rates decrease significantly over time and space, and the normalized mass loss from the source zone never reaches 1 for continuous sources due to the semi-infinite integral. The simulations also showed that disappearance times increase exponentially with increasing source radii and matrix porosity, and decrease with increasing aqueous-phase NAPL solubilities.</p> <p> The observations from the physical model experiments were very close to the simulated data at z = 0, validating the 3D mathematical models for this elevation. A plot of the observed vs simulated data did not reveal any trends, indicating that the majority of the differences can be attributed to experimental error. The experimental concentrations were below the method detection limit at depths of 3 and 6 cm however, indicating that either the experiments should have been conducted over a longer time period or a more sensitive analytical method should have been employed, to enable model validation at these depths.</p> / Thesis / Master of Applied Science (MASc)

In an attempt to reach a better understanding of the properties and critical behavior of non-equilibrium systems, we investigate the steady state properties of three simple models, variations of the prototype, the driven Ising lattice gas. Our first system studied is the bilayer model, a stack of two driven Ising lattice gases allowed to interact. We study this model using a very simple analytic approximation, the high temperature expansion. Building on existing simulation data and field theory results, our goal is to test how faithfully the series expansion can reproduce the Monte Carlo phase diagram. We find that the agreement between our calculations and the already reported simulations results is remarkably good. Next, we investigate the critical behavior of a two-dimensional Ising lattice gas driven into a non-equilibrium steady state, subject to a local modification of the dynamics, namely, having anisotropic attempt frequencies for exchanges along different spatial directions. We employ both Monte Carlo simulation techniques and a high temperature expansion approximation and find the phase diagram of the system, perform a finite-size scaling study in order to determine the universality class of the model and compare our simulation results with the phase diagram obtained using the high temperature expansion. We conclude that the bias in the jump rates does not affect the universal critical properties of the system: the modified model is in the same universality class as the driven Ising lattice gas. Our last objective concerns a different inroad into the study of non-equilibrium steady states. Instead of investigating a non-equilibrium steady state via indirect observables, such as correlation functions and order parameters, we seek to compute the steady state probability distribution directly. This is feasible only for systems with a small number of degrees of freedom. We chose to study a one-dimensional version of the so-called two-temperature kinetic Ising model. We solve the master equation exactly for a 1x6 system, and compare the full configurational probability distribution with its equilibrium counterpart. / Ph. D.