Abstract: TSG101 is a tumor susceptibility gene exhibits multiple biological function, including the regulation of cell progression, intracellular protein sorting and membrane trafficking, and transcription activity of nuclear recptor such as estrogen recptor. TSG101 contains an UBC domain which is homologous to that in ubiquitin conjugating E2 enzyme. However, it lacks an essential cysteine residue, which is essential for catalytic activity. Cellular protein ubiquitination serves as a signal for protein degradation or sorting into multivesicular body. UBC domain of TSG101 was proved to contain amino acid residues that are important for its interaction with ubquitin (residues V43, N46, D46 and F88) and PTAP sequence found in the late domain of HIV gag protein (residues Y63, M95, V141). SUMO is an ubquitin-like modifier which can modify cellular protein harbors £ZKXE amino acid sequence, thereby change its subcellular localization and biological activities. TSG101 protein contains K98, K243, K264 and K269 residues that localize in potential SUMO modification site. Our preliminary data indicated that TSG101 colocalize with SUMO in nucleus. It is interesting to know whether TSG101 is sumoylated, and its functional significance. In this thesis, a series of site-directed mutageneic mutant HA and GFP-tagged expression plasmids which contain mutation of the above mentioned functional related amino acid residues were constructed for future TSG101 functional studies.

Information science and technology fast development not only impact industry external competition environment, but also has the significant influence to the enterprise interior management. Along with network technology maturity, more and more enterprises Internet technical application in enterprise, thus constructs the construction enterprise exclusively intranet system, to unimpeded internal information, and provides each kind of convenient service and the function, then reduces the cost, to strengthened enterprise physique, to ensure the competitive advantage. On this occasion of the information explodes, no matter the society or individual, the lifelong study is the inevitable tendency, but constructs the construction using the enterprise interior network e-Learning to provide the multiplex study pipeline, may cause the knowledge management and study obtains more convenient and effectiveness. Steps while the whole world to the information, the science and technology and the government advocated the lifelong study, the enterprise promote the industry to encourage education, personally to take advanced courses become common practice, it reveal e-Learning potential to become the epoch-making education learning. However, from the view point of business management, lead-in e-Learning system incurs great expense. This research use the¡uUnified Theory of Acceptance and Use of Technology / UTAUT¡vas a foundation, from the large-scale traditional industry as a viewpoint, revises its scales, and use the way of lamination random sampling to carry on the questionnaire survey to the case study company, and obtains 180 effectively questionnaires. The main conclusion includes: To the case study company's staffs [1] Influence e-Learning system to accept a factor to be allowed to induce is "Performance Expectancy ", ¡§Effort Expectancy ", " Social Influence ¡§and " Facilitating Conditions " four items, different staff have different factor value degree. [2] The influence system use wish factor only has ¡§Performance Expectancy ", and " Facilitating Conditions " and so on two items; At the same time directly the behavior has the influence " Performance Expectancy " to "Actual use": The other two factors influence (Effort Expectancy and Social Influence) certainly does not reveal. [3] " Performance Influence" for the wish of uses and the actual behavior influence far surpass other factors.

This study discussed the relationships among employee personality, self-directed learning, and work performance. Samples are from people who serve in businesses, answer questionnaires with four main aspects, including Internal-External Scale, Self-Directed Learning Readiness Scale, Performance, and background data. The analysis results showed the following findings: 1. The differences of self-directed learning due to different personal background. a. Sex, age, education, department, level, work experience do not show significant differences on self-directed learning. b. The middle and high level manager are higher self-directed learning. c. People who had training in the past year are higher self-directed learning. d. People who had training or advanced study out of their own needs before are higher self-directed learning. 2. People with internal locus of control are higher self-directed learning. 3. People with internal locus of control have better performance. 4. People who are efficient and autonomous learner have better performance. 5. Employee¡¦s self-directed learning is influenced by locus of control, and it further influences performance. 6. Employee¡¦s self-directed learning is not influenced by his/her management level, and it won¡¦t influence performance further. 7. Employee¡¦s self-directed learning is influenced by his/her attending training in the last year, and it further influences performance. 8. Employee¡¦s self-directed learning is not influenced by his/her experience having training or advanced study out of his/her own needs before, and it won¡¦t influence performance further. According the findings, this research suggests: 1. Business can use personality measurements and Self-Directed Learning measurements as tools of selection. 2. Business should give employees more autonomy and resources assistance in the human development programs. 3. Business should give employees developmental opportunities in equality. 4. Employees should have the attitude being responsible to their own learning.

The Study of the Relationship among Organizational Climate Awareness, Self-directed Learning, and Job Involvement ¡V Taking One International Express Corporation as Example Abstract Organizational climate has an influence on the awareness of organization personnel, and its not only affect their mind, but also behavior. In the changing world, the competition between people has been expanded from one country to the whole world. Learning is necessary for people to keep their competition advantage, and the result of learning will affect the corporation operations, and also bring up the adult learning atmosphere. However, the study is focus on how does the corporation use this learning atmosphere to reinforce employees¡¦ job involvement, and use the organizational climate awareness affect employees¡¦ job involvement. The four types of organizational climate are leadership, organization structure, people relationship and responsibility which is this study trying to discuss the influence on self-directed learning and job involvement, also understand the self-directed learning mediating effect the relationship between organizational climate awareness and job involvement. After statistic analyzing, the results are summarize as following: 1. The different individual variables to organizational climate awareness, self-directed learning and job involvement come out a partly remarkable discrepancy. 2. Organizational climate awareness was positively significant to job involvement. 3. Self-directed learning was affected job involvement partly. 4. Organizational climate awareness was affected employee self-directed learning partly. 5. Self-directed learning was found to have a significant mediating effect between organizational climate and job involvement.

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Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (p. 69-73).

Thesis (M.S. in Applied Physics)--Naval Postgraduate School, June 2004. / Thesis advisor(s): William B. Colson. Includes bibliographical references (p. 49). Also available online.

This research explored the causal relationships among strategies, corporate structure, and performance of the largest U.S. non-financial firms using Directed Acyclic Graphs (DAGs). Corporate strategies and structure have been analyzed as major variables to influence corporate performance in management and organizational studies. However, their causal relationships in terms of which variables are leaders and followers, as well as the choices of variables to configure them, are controversial. Finding of causal relationships among strategic variables, structural variables, and corporate performance is beneficial to researchers as well as corporate mangers. It provides guidance to researchers how to build a model in order to measure influences from one variable to the other, lowering the risk of drawing spurious conclusions. It also provides managers a prospect of how certain important variables would change by making a certain strategic decision. Literatures from agency theory, transactional cost economics, and traditional strategic management perspective are used to suggest variables essential to analyze corporate performance. This study includes size and multi-organizational ownership hierarchy as variables to configure corporate structure. The variables to configure corporate strategies are unrelated and related diversification, ownership by institutional investors, debt, investment in R&D, and investment in advertisement. The study finds that most of the variables classified as corporate strategy and corporate structure variables are either direct or indirect causes of corporate accounting performance. Generally, results supports the relational model: corporate structure® corporate strategy® corporate performance. Ownership hierarchy structure, unrelated diversification, advertising expenses, and R&D intensity have direct causal influences on corporate accounting performance. Size and related diversification affected corporate accounting performance indirectly, both through ownership hierarchy structure. Theoretical causal relationships from agency theory are less supported than those from transaction cost economics and traditional strategic management perspective. Further my study suggests that, in general, good corporate performance in 1990s was mainly achieved by internal expansion through investment in R&D and advertisement, rather than external expansion of firms through unrelated diversification, related diversification, and expansion of ownership hierarchy.

The current thesis explored the intentional forgetting of different types of facial expression (Angry, Neutral, Happy) within the item-method directed forgetting paradigm (Experiments 1-4). Also, as a manipulation check, Experiment 5 obtained the subjective ratings of valence and arousal for the different types of facial expression used in the previous four Experiments. In summary, a significant directed forgetting effect occurred for Neutral facial expressions; however, a significant directed forgetting effect did not consistently occur for emotional facial expressions (e.g., there was no directed forgetting effect for Angry facial expressions in Experiments 2 and 3, or Happy facial expressions in Experiment 3). These findings are discussed in terms of encoding time as well as valence and arousal, and how these two factors modulate the effect of emotional facial expression on the ability to intentionally forget.

Directed metalation reactions are an interesting area of synthetic chemistry. They provide a powerful regiospecific method for functionalizing complex aromatic rings. Presently, directed ortho metalations are well understood. However, there has been very little progress in the remote metalation of naphthalene derivatives. Presently the peri lithiation of naphthalamides is a reaction that has not been solved to a level that allows for it to be deployed in a vast number of synthetic schemes. The processes by which peri functionalized naphthalamides are present obtained required several steps and give poor yields. Previously attempts directed toward the peri lithiation in the Schwan lab have met with little success. Herein experiments were preformed to understand why the pervious attempts failed and other experiments were performed in an effort to acheive the peri lithiation of a specific naphthalamide. The mechanism of previous chemical observations was understood by trapping experiments and clearly demonstrated how problematic acidic sites can interfere with intended directed metalation. The acidic site was sterically hindered by employing strategic protecting groups. However, the steric demand of the protecting groups considered was not sufficient to eliminate the problematic acidic site. This led to the consideration of an alternative strategy for the peri lithiation by removing the acidic center of the specific substrate. This however, resulted in remote addition of lithiating reagents to the naphthalamide and clearly showed that several naphthalamides are not a suitable directed metalation group for the peri lithiation.