Disrupted in Schizophrenia 1 (DISC1) is a well supported risk factor for schizophrenia, bipolar disorder and major recurrent depression. DISC1 is a multifunctional multicompartmentalised scaffold protein with essential roles in neuronal proliferation, differentiation, migration and integration. DISC1 also modulates pathways of vital importance for neuronal signalling and plasticity. One of the major hypotheses for the cause of psychiatric illness is N-methyl-D-aspartate (NMDA) receptor hypofunction. It was observed that NMDA receptor antagonists can induce symptoms of schizophrenia in unaffected individuals, and exacerbate symptoms in patients with schizophrenia. Recent work in our laboratory showed that DISC1 complexes with NMDA receptors within the cell body and at synapse of neurons. Here I studied whether DISC1, or DISC1 missense variants, affect the trafficking of NMDA receptors. This was done by quantifying surface NMDA receptor expression in the presence of DISC1 or variant DISC1. I found that one common variant, 607F, causes a significant reduction in surface expressed NMDA receptors. I went on to show that DISC1 reduces the number of internalised receptors associating with early RAB5-containing endosomes. This indicates that DISC1 may be involved in the trafficking and recycling of NMDA receptors, a process that may be affected by the missense DISC1 variant 607F. Further to this I studied the effects on NMDA receptor trafficking of DISC1 pathway partners Nuclear Distribution Element 1 (NDE1) and Trafficking-protein kinesin binding 1 (TRAK1), both regulators of neuronal intracellular trafficking. Phosphorylation of NDE1 at T131 has been shown to be modulated by DISC1. Using phospho-mimic and phospho-dead NDE1 expression constructs I observed a significant reduction in the surface-expressed NMDA receptors in cells expressing the phospho-mimic form of NDE1. NDE1 may therefore be involved in the trafficking of NMDA receptors, and this role may be modulated by phosphorylation of NDE1. Finally, TRAK1 was shown to associate robustly with the GluN2B subunit, and to decrease the surface expression of NMDA receptors, most likely by sequestering them. The TRAK1-induced GluN2B sequestration may be an artefact, but the association of the trafficking molecule TRAK1 with this subunit may point towards a role in NMDA receptor trafficking. These proteins have been shown to associate with each other and may form a complex in order to traffic NMDA receptors. Disruption of this complex by defective DISC1 expression may affect NMDA receptor trafficking. In the brain this could conceivably contribute to NMDA receptor hypofunction and the development of psychiatric illness.

A traffic network can be disrupted by work zones and incidents. Calculating diversion rate is a core issue for estimating demand changes, which is needed to select a suitable work zone configuration and work schedule. An urban network can provide multiple alternative routes, so traffic assignment is the best tool to analyze diversion rates on network level and the local level. Compared with the results from static traffic assignment, dynamic traffic assignment predicts a higher network diversion rate in the morning peak period and off-peak period, a lower local diversion rate in the morning peak period. Additionally, travelers may benefit from knowing real-time traffic condition to avoid the traffic incident areas. Deploying variable message signs (VMSs) is one possible solution. One key issue is optimizing locations of VMSs. A planning model is created to solve the problem. The objective is minimize total system travel time. The link transmission model is used to evaluate the performance of the network, and bounded rational behavior is used to represent drivers' response to VMSs. A self-adapting genetic algorithm (GA) is formulated to solve the problem. This model selects the best locations to provide VMSs, typically places are that allow travelers to switch to alternative routes. Results show that adding more VMSs beyond a certain threshold level does not further reduce travel time. / text

In a large Scottish family a high incidence of schizophrenia, bipolar disorder and major depressive disorder co-segregates with a balanced autosomal translocation (t(1;11)(q42.1;q14.3). The translocation disrupts Disrupted-in-Schizophrenia-1 (DISC1) and DISC2 on chromosome 1, and DISC1FP1 (Disrupted-in-Schizophrenia-Fusion-Partner-1), also known as Boymaw, on chromosome 11. DISC1 is a leading candidate gene for major mental illness and is involved in neurodevelopment and cellular signalling, whilst DISC2 and DISC1FP1 are apparently non-coding RNA genes that undergo alternative splicing and that are expressed in the brain. This thesis aimed to investigate putative mechanisms of pathogenesis that may result from the t(1;11), with the hope that pathogenic mechanisms identified in the t(1;11) pedigree might shed light upon mechanisms conferring risk for psychiatric illness in the wider population. Previous work had identified DISC1/DISC1FP1 chimeric transcripts in t(1;11)-family derived lymphoblastoid cell lines. The detected transcripts include CP60 and CP69 which encode DISC1 aa1-597 plus an additional 60 or 69 amino acids from DISC1FP1, respectively. In this thesis a novel DISC1/DISC1FP1 transcript, CP1, was identified in t(1;11) lymphoblastoid cell lines. The CP1 transcript encodes DISC1 aa1-597 plus one glycine. A truncated form of DISC1 comprising aa1-597 was previously suggested to be a putative product of the translocation and, as such, has been the focus of multiple studies. The identification of the CP1 species is of interest as it differs from DISC1 aa1-597, by only a glycine. As glycines are simple uncharged aa’s, it is likely that these two DISC species share similar properties. In vitro exogenous expression of the three DISC1/DISC1FP1 protein species in both COS-7 and primary neuron cultures revealed contrasting cellular phenotypes. CP1 showed a diffuse cellular localisation pattern with cells containing readily visible tubular mitochondria. This is indistinguishable from the staining pattern of DISC1 aa1-597, highlighting the high degree of similarity between these species. CP60 and CP69, however, appeared to be clustered in the perinuclear region of the cell. Initial staining attempts with MitoTracker Red to visualise mitochondria in CP60 and CP69 expressing cells resulted in fewer than 30% of cells being stained. In those that did stain, the mitochondria appeared clustered. The absence of MitoTracker Red staining in mitochondria may be due to the loss of the mitochondrial membrane potential, Δψm. The adoption of a co-staining protocol with antibodies for mitochondrial proteins enabled the visualisation of mitochondrial structure in all of the cells exogenously expressing CP60 and CP69. All of these mitochondria possessed a clustered morphology, with which CP60 and CP69 expression was substantially co-localised. To see if MitoTracker staining was perturbed, in t(1;11) lymphoblastoid cell lines, as may occur if the DISC1/DISC1FP1 chimeras are expressed endogenously, the fluorescence of MitoTracker Red staining was investigated by FACS. Pooled analysis of experimental replicates revealed a negative result, with MitoTracker Red staining in t(1;11) lymphoblastoid cell lines not differing from controls. These findings indicate a need for further research using the mitochondrial membrane potential, Δψm as a metric as this would enable variations in mitochondrial mass to be accounted for. Prior to my arrival, an expression microarray had been carried out on lymphoblastoid cell line cDNA to assess gene expression differences resulting from the t(1;11). In order to identify putative pathogenic mechanisms, I carried out functional enrichment analysis of the expression array data using multiple analysis programs. Several programs detected dysregulation of the cell cycle and enrichment of altered expression of genes involved in the immune response and inflammation in t(1;11) carriers. The use of a rare variant investigative paradigm in this thesis furthers understanding of the putative pathogenic mechanisms that might act to increase risk for psychiatric illness in t(1;11) carriers. Moreover, it may aid the biological understanding of the aetiology of psychiatric illness in the general population. As such, improved understanding of the mechanisms of risk in the t(1;11) pedigree may eventually lead to the development of better treatments. In the intervening time since some of the research for thesis was published, two studies have emerged that may serve to highlight potential mechanisms of pathogenic action mediated by CP60 and CP69 expression. It has recently been observed that WT-DISC1 couples to the adaptor protein TRAK1 and the mitochondrial membrane anchor Miro1, which are part of the mitochondrial transport complex (Ogawa et al, 2014; Norkett et al, 2016). Furthermore, the exogenous expression of CP60 impairs bidirectional mitochondrial trafficking (Norkett et al, 2016). This suggests that CP60 expression may impair interactions with TRAK1 and Miro1. Given the sequence homology between CP60 and CP69, mitochondrial transport deficits also likely arise with CP69 expression. It is therefore possible that the exogenously expressed CP60 and CP69 proteins could be docked on stationary mitochondria, which may contribute to the clustered expression patterns observed.

The Disrupted-In-Schizophrenia 1 (DISC1) gene is one of the most established risk genes for psychiatric illness currently being studied, having originally been identified as being directly disrupted by a balanced chromosomal translocation that cosegregates with schizophrenia and other major mental illness a large Scottish family. The DISC1 protein is believed to act as a molecular scaffold within the cell, binding to a large number of other proteins. Three of these protein interactors, Phosphodiesterase 4B (PDE4B), Nuclear Distribution Factor E (Aspergillus nidulans)-homologue 1 (NDE1) and NDE-Like 1 (NDEL1) all have evidence implicating them as schizophrenia-related proteins in their own right. NDE1 and NDEL1 are highly similar proteins which are known to play cellular roles including microtubule function and mitosis. Their orthologues have also been shown to be important in neurodevelopment within the mouse brain. To date, most work in the literature has investigated NDEL1, with few focusing on NDE1. In the thesis, I first seek to establish a basic biology for NDE1 by the identification of splice variants expressed in the brain, establishing cellular localisation patterns within the cell and investigating NDE1 multimerisation. The relationship between NDE1 and NDEL1 is also investigated, with the two being found to form complexes together and to have partially over-lapping expression patterns within the cell. That NDE1 and DISC1 directly interact is confirmed. The relationship between NDE1 and PDE4B is then investigated, with the two proteins found to complex within the cell. Additionally, it is shown that NDE1 can be phosphorylated by protein kinase A (PKA). This kinase is cAMP dependant, and is thus indirectly regulated by the cAMP-degrading action of PDE4B protein. Attempts to map and analyse the effect of this phosphorylation on NDE1 are made.

Deficits in a number of cognitive domains have been associated with core symptoms of schizophrenia, including working memory, attention, motor skills, reaction time, episodic memory and executive function. Bipolar Disorder is also associated with cognitive impairment; however the level of impairment appears to be less severe than that seen in schizophrenia. A translocation (t(1;11)) containing the Disrupted-in-Schizophrenia 1 (DISC1) gene has been found to be highly associated with schizophrenia, bipolar disorder and major depressive disorder. As such, this gene has been the focus of much research and to date DISC1 has been found to be associated with brain development, brain structure and the glutamate system - all key factors in current models of schizophrenia and affective disorders. The aim of this PhD is to identify cognitive domains that are differentially impaired or unimpaired in a large Scottish family, some of whom carry this rare DISC1 variant, a balanced translocation (t (1;11) (q 42; q14.3)), that segregates with schizophrenia and affective disorders, as well as psychiatric patients with schizophrenia and bipolar disorder and healthy control subjects. All participants have undergone standardised cognitive assessments to measure premorbid I.Q. (NART), current I.Q. (WASI) verbal memory, working memory, verbal fluency, processing speed, motor skills, executive function (BACS) and selected CANTAB tasks to assess simple and five-choice reaction time. Polygenic risk profile scores and self-report questionnaire data have also been investigated. Results indicate an impact of the DISC1 t(1;11) translocation on general intelligence and attention and processing speed. Significant differences were also identified between DISC1 t(1;11) carriers and non-carriers on self-report questionnaire data. Mean scores for polygenic risk for bipolar disorder were significantly different between DISC1 t(1;11) carriers and non-carriers and polygenic risk for schizophrenia was significantly associated with symptom severity, as measured by the Positive and Negative Symptom Scale (PANSS). Within the patient groups, a measure of processing speed (the token motor task) was found to be significantly different between those with schizophrenia and bipolar disorder and there was also a trend for attention and processing speed. As expected, I.Q. was significantly different between patients and control participants. Clinical ratings were significantly associated with neuropsychological and self-report measures. Polygenic risk for major depressive disorder was found to be significantly associated with impaired general intelligence (current IQ) and slowed reaction time in patients who were not currently depressed, suggesting there may be genetic risk markers in this population which impact on cognition. This is a novel finding and further suggests the possibility of a biological component related to the genetics of depression. In conclusion, and in line with the literature, psychosis has a negative impact on cognition with reduced performance across several neuropsychological tasks between patient groups, with schizophrenia patients performing worse than patients with bipolar disorder and both patient groups performing worse than healthy control participants. Cognition is markedly more impaired in DISC1 t(1;11) translocation carriers and especially in those with psychosis. The DISC1 t(1;11) translocation and psychosis may therefore confer a “double hit” on cognition - in addition to psychosis itself - which is known to impair cognitive function, significantly increasing the level of cognitive impairment and increasing the risk for psychosis in general.

Bakgrund Sömnstörningar och fragmenterad sömn förekommer i stor utsträckning bland patienter som vårdas på intensivvårdsavdelningar. Otillräcklig sömn kan leda till längre återhämtningstid och försämrad sömnkvalitet kan kvarstå i flera månader efter utskrivning och till följd av det avsevärt försämra patienters livskvalitet. Syfte Syftet med denna litteraturöversikt var att undersöka vilka omvårdnadsåtgärder som kan främja en god nattsömn för intensivvårdspatienter.  Metod Litteraturöversikt med systematisk ansats baserad på kvantitativa studier. En systematisk litteratursökning gjordes i databaserna PubMed och Cinahl vilket resulterade i 16 artiklar. Artiklarna kvalitetsgranskades sedan och resultatet sammanställdes genom en narrativ analys.  Resultat Totalt inkluderades 16 randomiserade kontrollerade studier som undersökte olika interventioner för att främja sömn. Omvårdnadsåtgärder som sovmask samt öronproppar + sovmask framstod som de mest effektiva interventionerna för att främja god sömnkvalitet. Även aromaterapi och meditation med hjälp av VR ledde till förbättrad sömn. Slutsats För att optimera patienters möjlighet till återhämtning är det viktigt att patienterna får möjlighet till god sömn under vårdtiden. Flertalet omvårdnadsåtgärder som tas upp i litteraturöversikten skulle kunna implementeras på intensivvårdsavdelningar för att förbättra patienters sömnkvalitet.

Topic: Motion Composition for children with Autistic Spectrum Disorder Objectives: The aim of this thesis has been to find out the degree of mastery for individual performance of Motion Composition performed by children with Autistic Spectrum Disorder. Methods: Observation method was supplemented with video-recording that was conducted monthly. This was used to determine the degree of self-reliance. Also, feedback from other individuals has been used. Results: Outcomes of this survey indicate that children with Autistic Spectrum Disorder (ASD) are able to individually perform Motion Composition. Some children need more time to perform perfectly, but they are able to memorize components of Motion Composition, arrange them in the correct order and after some rehearsal they are able to perform Motion Composition correctly. The level of performance for individual components is raised proportionally when children can see visual execution of each element and also hear its description. Evaluated results of a second scientific question, which was related to the level of performance difficulty of individual components of Motion Composition for children with ASD, have brought unanticipated results. We anticipated that isometric exercises would be the most difficult components of Motion Composition. According...

Doctor of Philosophy / This study considers the adequacy of explanatory accounts of recruitment to New Religious Movements [NRMs], defined by their doctrinal innovation or importation from another culture, and formed around a charismatic leader. It considers the coercive persuasion paradigm [brainwashing] which assumes no predisposing features of those who become involved in NRMs and a sociological account by Snow Zurcher and Ekland-Olsen (1980) which seeks to redress overly exclusive attention to psychological variables by emphasising the importance of structural variables such as the existence of 'discretionary time' and having a friend involved in the NRM. It is suggested that a psychological focus in explaining movement involvement need not entail a de-emphasis of the relevance of current life circumstances, such as social bonds, and life stress, nor a failure to acknowledge the importance of the group's ideology in lending definition to a person’s felt difficulties. A new model of personal change is proposed, termed the Rites de Passage model, which entails the disruption, transition and reincorporation of a socially sustained sense of identity and suggests conversion can be viewed as an example of re-socialisation. The historical lineage of the model is traced from Van Gennep's (1908) anthropological work to studies of brainwashing in the work of Schein (1957) and Lifton (1961). Since the emphasis is on the profile of a seeker, specific focus is placed on the early phases of this process where disruption occurs in existing coping techniques and social supports as a result of disruptive life events, and consideration is given to other relevant precursors of movement involvement. Lofland and Stark's (1965) model forms the conceptual framework from which literature regarding differences in life stress, social bonds, prior behavioural involvement in NRMs, and prior cognitive spiritual orientation can be addressed. The work of Galanter (1980, 1989), Barker (1981, 1984), Heirich (1977) and Snow and Phillips (1980) provides substantial evidence for the existence of pre-existing differences between affiliates (who make contact with such movements) and nonaffiliates (who do not). In this thesis two facets of differential involvement are addressed: i) why does one individual rather than another become involved ii) with a given genre of movement rather than another? The Rites de Passage model proposed here, which is a modified version of Lofland and Stark's (1965) account of cult conversion, is tested placing NRMs in a comparative context with a secular self-help agency: a therapy group. People with disrupted social identities might seek movement involvement, but what distinguishes whether they seek out a secular or spiritual movement, and if spiritual – what determines the appeal of eastern or western spiritual groups? To explore these questions, four groups of affiliates to three different eastern NRMs are compared to a therapy group, (Richardson and Kilbourne, 1984), two control groups (a student sample, and a sample from the general population) and a western NRM. There are 160 subjects overall, who completed a battery of questionnaires at point of first contact with the movement, to distinguish the precursors for movement involvement from the sequelae. Exceptions to this prospective data collection were the western NRM and the inclusion of a graduate rebirthing group. The latter was deliberately included to facilitate pre-involvement and post-involvement comparisons. The former's adept status was due to the leader's reluctance to burden new members with a three hour test battery. Measures were taken regarding life events and their psychological impact using Henderson, Byrne and Ducan-Jones (1981) recent life events inventory and impact scales using a twelve month time frame. A modified version of the Interview Schedule for Social Interaction (by Henderson et al, 1981) was used to assess the availability and adequacy of acquaintance-level and intimate bonds in the recent past. Mental health was assessed using Galanter's (1980) General Wellbeing Scale and Tellegen's Multidimensional Personality Questionnaire (1982). Recollections of early family relations were assessed using Likert scales derived from the detailed comparative study by Ullman (1982) which suppported the psychoanalytic hypothesis regarding troubled early parental relations, suggesting that current life difficulties re-evoked early life problems. Since disruption is seen as a necessary but not sufficient condition for movement involvement (Greil, 1977) the therapy and eastern groups were not expected to differ from each on the disruption and loneliness measures, and they did not differ. They were expected to have experienced more disruption in greater isolation than the control groups and those already belonging to a spiritual group – namely the western NRM. The therapy and eastern NRM groups did differ from the others in these respects. The value-added form of the model merely specifies that a substrate of stress and disruption suffered in relative isolation and loneliness will increase the probability that some for of social agency will be sought. Disruption experienced in relative loneliness is the first component of differential recruitment to movement involvement, or ‘differential openness’ as it is termed here. So the brainwashing model does not hold as there are differences between those drawn to movements compared to control groups. Do personality differences contribute to which movement genre appeals? A strikingly different personality profile emerged of those drawn to eastern NRMs. Differences were predicted and found between the eastern groups on the one hand and therapy group, control groups and western group on the other, when personality variables were considered. Relevant features of the profile included: a lack of traditionalism, a challenging attitude to conventional authority (assessed by Ray's (1971) balanced F scale) and absorption - a tendency to experience perceptual phenomena indicative of an absorptive or mystical tendency (Tellegen's MPQ was used to assess this personality feature). The eastern groups have a personality profile of being: unconventional, somewhat impulsive and highly absorptive in perceptual style. This profile distinguished them from all other groups. When the additional feature of the model was considered the profile of a potential seeker was more strongly delineated: the consonance between an individual’s intensity and orientation of spiritual beliefs and the orientation of movement ideology was highly influential. This was assessed by a spiritual orientation scale [the SOS] developed by the author across three pilot studies using Coombs Unfolding Technique (Coombs, 1964) to produce a metric ordinal scale which assesses general spiritual beliefs (which underlie any spiritual worldview), eastern and western spiritual beliefs. A major finding of the study was that a markedly distinctive feature of those drawn to NRMs is a spiritual orientation consonant with that of the movement approached. The SOS revealed a strongly demarcated pre-existing eastern spiritual orientation in those drawn to make contact with Eastern NRMs, which set them apart significantly from all other groups. The Western NRM, (already members of their group) had a western spiritual orientation, to the exclusion of an eastern orientation, while the eastern groups were more eclectic. Both eastern and western NRMs were spiritually more intense on the general spiritual items of the SOS, suggesting these items are central to any spiritual worldview. All of the major predictions of the Rites de Passage model were supported. The model provides a welcome link between a sociological and psychological focus on movement involvement. The systematic differences between affiliates and non-affiliates of NRMs at point of first contact, suggest (contra contemporary brainwashing models, though not the sophisticated models of Schein and Lifton) that recruitment is unlikely to be completely due to NRM design: the results suggest participants are likely to be interested and consenting. In summary, it is shown that those drawn to New Religious Movements of an eastern kind are indeed non-traditional, have a high incidence of recent life events and suffer a sense of community isolation, and loneliness which are considered as factors which might lead a person to modify an unfulfilling lifestyle. A portrait of a seeker is lightly (sketched against a background of this dissatisfaction) which includes personality variables like an impulsive, present-oriented pleasure/pain regulatory style, being high on absorption -a mystical perceptual style, and having both an intensity and a congruence of spiritual orientation with that of the ideology of the movement approached. These are considered potential influences on the genre of movement contacted, and are suggested as explanatory of the second facet of differential openness to movement involvement. Disruption sets a person seeking; personality shapes to which appeals s/he is open. The relative privilege of the Western NRM in terms of reduced stress, availability of community and intimate social support suggests that involvement does provide a relief effect, though caution must be exercised in interpreting this difference as these groups differ in membership status and spiritual orientation. The distress and neediness of those contacting movements for the first time is apparent, which suggests that movement contact might be a response to felt dissatisfaction interpreted within a spiritual worldview. An eastern spiritual worldview is a highly significant distinguishing feature of affiliates, and is the final phase of the Rites de Passage Model. Speculative theoretical consideration is offered of the data's implications for a psychoanalytic consideration of movement involvement, in the light of Cushman (1986), Deutsch (1983), Halperin (1983) Doi (1971) and Kohut's (1977). Theory and research is adumbrated concerning differential openness to charismatic appeal.

Doctor of Philosophy / This study considers the adequacy of explanatory accounts of recruitment to New Religious Movements [NRMs], defined by their doctrinal innovation or importation from another culture, and formed around a charismatic leader. It considers the coercive persuasion paradigm [brainwashing] which assumes no predisposing features of those who become involved in NRMs and a sociological account by Snow Zurcher and Ekland-Olsen (1980) which seeks to redress overly exclusive attention to psychological variables by emphasising the importance of structural variables such as the existence of 'discretionary time' and having a friend involved in the NRM. It is suggested that a psychological focus in explaining movement involvement need not entail a de-emphasis of the relevance of current life circumstances, such as social bonds, and life stress, nor a failure to acknowledge the importance of the group's ideology in lending definition to a person’s felt difficulties. A new model of personal change is proposed, termed the Rites de Passage model, which entails the disruption, transition and reincorporation of a socially sustained sense of identity and suggests conversion can be viewed as an example of re-socialisation. The historical lineage of the model is traced from Van Gennep's (1908) anthropological work to studies of brainwashing in the work of Schein (1957) and Lifton (1961). Since the emphasis is on the profile of a seeker, specific focus is placed on the early phases of this process where disruption occurs in existing coping techniques and social supports as a result of disruptive life events, and consideration is given to other relevant precursors of movement involvement. Lofland and Stark's (1965) model forms the conceptual framework from which literature regarding differences in life stress, social bonds, prior behavioural involvement in NRMs, and prior cognitive spiritual orientation can be addressed. The work of Galanter (1980, 1989), Barker (1981, 1984), Heirich (1977) and Snow and Phillips (1980) provides substantial evidence for the existence of pre-existing differences between affiliates (who make contact with such movements) and nonaffiliates (who do not). In this thesis two facets of differential involvement are addressed: i) why does one individual rather than another become involved ii) with a given genre of movement rather than another? The Rites de Passage model proposed here, which is a modified version of Lofland and Stark's (1965) account of cult conversion, is tested placing NRMs in a comparative context with a secular self-help agency: a therapy group. People with disrupted social identities might seek movement involvement, but what distinguishes whether they seek out a secular or spiritual movement, and if spiritual – what determines the appeal of eastern or western spiritual groups? To explore these questions, four groups of affiliates to three different eastern NRMs are compared to a therapy group, (Richardson and Kilbourne, 1984), two control groups (a student sample, and a sample from the general population) and a western NRM. There are 160 subjects overall, who completed a battery of questionnaires at point of first contact with the movement, to distinguish the precursors for movement involvement from the sequelae. Exceptions to this prospective data collection were the western NRM and the inclusion of a graduate rebirthing group. The latter was deliberately included to facilitate pre-involvement and post-involvement comparisons. The former's adept status was due to the leader's reluctance to burden new members with a three hour test battery. Measures were taken regarding life events and their psychological impact using Henderson, Byrne and Ducan-Jones (1981) recent life events inventory and impact scales using a twelve month time frame. A modified version of the Interview Schedule for Social Interaction (by Henderson et al, 1981) was used to assess the availability and adequacy of acquaintance-level and intimate bonds in the recent past. Mental health was assessed using Galanter's (1980) General Wellbeing Scale and Tellegen's Multidimensional Personality Questionnaire (1982). Recollections of early family relations were assessed using Likert scales derived from the detailed comparative study by Ullman (1982) which suppported the psychoanalytic hypothesis regarding troubled early parental relations, suggesting that current life difficulties re-evoked early life problems. Since disruption is seen as a necessary but not sufficient condition for movement involvement (Greil, 1977) the therapy and eastern groups were not expected to differ from each on the disruption and loneliness measures, and they did not differ. They were expected to have experienced more disruption in greater isolation than the control groups and those already belonging to a spiritual group – namely the western NRM. The therapy and eastern NRM groups did differ from the others in these respects. The value-added form of the model merely specifies that a substrate of stress and disruption suffered in relative isolation and loneliness will increase the probability that some for of social agency will be sought. Disruption experienced in relative loneliness is the first component of differential recruitment to movement involvement, or ‘differential openness’ as it is termed here. So the brainwashing model does not hold as there are differences between those drawn to movements compared to control groups. Do personality differences contribute to which movement genre appeals? A strikingly different personality profile emerged of those drawn to eastern NRMs. Differences were predicted and found between the eastern groups on the one hand and therapy group, control groups and western group on the other, when personality variables were considered. Relevant features of the profile included: a lack of traditionalism, a challenging attitude to conventional authority (assessed by Ray's (1971) balanced F scale) and absorption - a tendency to experience perceptual phenomena indicative of an absorptive or mystical tendency (Tellegen's MPQ was used to assess this personality feature). The eastern groups have a personality profile of being: unconventional, somewhat impulsive and highly absorptive in perceptual style. This profile distinguished them from all other groups. When the additional feature of the model was considered the profile of a potential seeker was more strongly delineated: the consonance between an individual’s intensity and orientation of spiritual beliefs and the orientation of movement ideology was highly influential. This was assessed by a spiritual orientation scale [the SOS] developed by the author across three pilot studies using Coombs Unfolding Technique (Coombs, 1964) to produce a metric ordinal scale which assesses general spiritual beliefs (which underlie any spiritual worldview), eastern and western spiritual beliefs. A major finding of the study was that a markedly distinctive feature of those drawn to NRMs is a spiritual orientation consonant with that of the movement approached. The SOS revealed a strongly demarcated pre-existing eastern spiritual orientation in those drawn to make contact with Eastern NRMs, which set them apart significantly from all other groups. The Western NRM, (already members of their group) had a western spiritual orientation, to the exclusion of an eastern orientation, while the eastern groups were more eclectic. Both eastern and western NRMs were spiritually more intense on the general spiritual items of the SOS, suggesting these items are central to any spiritual worldview. All of the major predictions of the Rites de Passage model were supported. The model provides a welcome link between a sociological and psychological focus on movement involvement. The systematic differences between affiliates and non-affiliates of NRMs at point of first contact, suggest (contra contemporary brainwashing models, though not the sophisticated models of Schein and Lifton) that recruitment is unlikely to be completely due to NRM design: the results suggest participants are likely to be interested and consenting. In summary, it is shown that those drawn to New Religious Movements of an eastern kind are indeed non-traditional, have a high incidence of recent life events and suffer a sense of community isolation, and loneliness which are considered as factors which might lead a person to modify an unfulfilling lifestyle. A portrait of a seeker is lightly (sketched against a background of this dissatisfaction) which includes personality variables like an impulsive, present-oriented pleasure/pain regulatory style, being high on absorption -a mystical perceptual style, and having both an intensity and a congruence of spiritual orientation with that of the ideology of the movement approached. These are considered potential influences on the genre of movement contacted, and are suggested as explanatory of the second facet of differential openness to movement involvement. Disruption sets a person seeking; personality shapes to which appeals s/he is open. The relative privilege of the Western NRM in terms of reduced stress, availability of community and intimate social support suggests that involvement does provide a relief effect, though caution must be exercised in interpreting this difference as these groups differ in membership status and spiritual orientation. The distress and neediness of those contacting movements for the first time is apparent, which suggests that movement contact might be a response to felt dissatisfaction interpreted within a spiritual worldview. An eastern spiritual worldview is a highly significant distinguishing feature of affiliates, and is the final phase of the Rites de Passage Model. Speculative theoretical consideration is offered of the data's implications for a psychoanalytic consideration of movement involvement, in the light of Cushman (1986), Deutsch (1983), Halperin (1983) Doi (1971) and Kohut's (1977). Theory and research is adumbrated concerning differential openness to charismatic appeal.

This qualitative phenomenological study was conducted in order to better understand the multiple multicultural educational experiences TCKs have as a result of their unwanted/unpredictable international moves. The transcendental approach included semistructured face-to-face interviews based on a questionnaire with 12 TCK students at an international school in Korea. Students were asked to share their emotional, academic, and social experiences. The three research questions underpinning the questionnaire were: What are the emotional experiences of TCK high school students at a Korean international school who had multiple school disruptions? What are the academic experiences of TCK high school students at a Korean international school who had multiple school disruptions? What are the social experiences of TCK high school students at a Korean international school who had multiple school disruptions? The participants had attended at least three different schools K-12 and had attended schools in at least two different countries. Results revealed that although TCKs are fearful of moving to a new school in a new country they are excited about the new experiences they will have as they transition to a new place and new school. Academically, the International Baccalaureate Programme is the curriculum TCKs prefer and find the most suitable to their international experiences. The most unique experiences TCKs face moving from one school to another in a different country revolve around relationships. The most difficult part about moving is leaving friends and family behind. While TCKs moving to a new school in a new country face complex challenges forming close relationships, they are open-minded and are able to relate to people from a variety of different backgrounds and nationalities.