This diploma thesis focuses on a DLP implementation within a specific organization. The thesis contains current situation analysis and provides decision for choice of the most suitable DLP based on the analysis findings. Eventually describes a real implementation of the chosen DLP system within the organization.

Building upon previous research on Digital Light Projection (DLP) 3D printing for microfluidics, in this thesis I performed the detailed design and fabrication of a novel DLP 3D printer to increase resolution and device footprint flexibility. This new printer has a pixel resolution twice that of our group’s previous printers (3.8 μm vs 7.6 μm). I demonstrated a new state of the art for minimum channel width, reducing the minimum width to 15 μm wide (and 30 μm tall). This is an improvement over the previous smallest width of 20 μm. This printer also has the capacity to perform multiple spatially distinct exposures per printed layer and stitch them into one interconnected device. Image stitching enables printing devices with identical build areas to previous printers, and with smaller pixel pitch. I pursued validation of this stitching capacity by fabricating channel devices with features crossing the stitched image boundary, with the goal of printing channels that would flow fluid consistently and without leaking. To accomplish this, I began by characterizing the print parameters for successfully printing single microfluidics channels across the stitched image boundary, and then I explored the sensitivity of my method to multiple crossings of the image boundary by printing a stacked serpentine channel that crossed the stitched image boundary 392 times. This demonstrated that an arbitrary number of stitched boundary crossings are feasible and thus a high degree of complex device component integration across these boundaries is also possible. These developments will be useful in future research and design of 3D printed microfluidic devices.

Le développement des senseurs protéiques et des outils optogénétiques au cours des dernières années a donné une place particulière à la microscopie pour l’étude des processus moléculaires in-vivo. L’équipe « Nano-optique et physiologie intégrée » développe des montages optiques originaux pour exploiter ces nouveaux outils chez le petit animal vivant en collaboration avec des neurobiologistes. Nous nous intéressons en particulier à l’organisation cellulaire et à l’activité des réseaux neuronaux impliqués dans la mémorisation associative olfactive de la drosophile. En amont, mon travail de thèse a été de mettre en place un microscope grand champ, basé sur le principe de la microscopie à HiLo, permettant l’acquisition rapide de sections optiques et la reconstruction tridimensionnelle de réseaux neuronaux. Après avoir prouvé la pertinence de l’approche choisie lorsqu’elle est associée aux outils génétiques permettant un marquage sélectif des neurones, le cœur de mon travail fut le développement d’un montage original permettant d’atteindre les objectifs de résolution spatiale et de vitesse. Son originalité se situe dans l’utilisation de la technologie des matrices de micro-miroirs (DLP) pour structurer l’illumination. Ce système de micro-miroirs pilotables peut moduler le faisceau d’une source LED haute puissance à haute cadence. Dans une seconde partie, j’ai caractérisé ce microscope et réalisé de premières expériences in-vivo avec les développements spécifiques nécessaires à ces expériences. En particulier, en utilisant un rapporteur protéique calcique fluorescent, GCamP3, j’ai montré que l’on pouvait suivre, dans des régions ciblées du cerveau, la réponse à des stimulations physiologiques à cadence vidéo. / In the last decades, optogenetic and protein reporter development have given a special place to optical microscopy for in-vivo investigation of biological molecular processes. Our team, “Nano-optics and integrated physiology”, develops optical set-ups to take advantage of these tools on small living animals, in collaboration with neurobiologists. We are particularly interested both in the cellular organization and neural activity involved in the olfactory memory formation in drosophila. Upstream to these investigations, my PhD research aimed at developing a new implementation for wide-field microscopy based on the HiLo concept. The new design took advantage of the micro-mirror array technology (DLP) to structure the illumination. This system can modulate the beam made by a high power LED illumination with high acquisition rates. I characterized this microscope and realized preliminary in-vivo experiments with specific developments made for physiological experiments under the microscope. Thus, I demonstrated both high spatial resolution imaging and a tenfold increase of speed with respect to confocal microscopy. I reached acquisition rates compatible with 3D monitoring of specific neural networks.

The growing interest that multimedia processing has experimented during the last decade is motivating processor designers to reconsider which execution paradigms are the most appropriate for general-purpose processors. On the other hand, as the size of transistors decreases, power dissipation has become a relevant limitation to increases in the frequency of operation. Thus, the efficient exploitation of the different sources of parallelism is a key point to investigate in order to sustain the performance improvement rate of processors and face the growing requirements of future multimedia applications. We belief that a promising option arises from the combination of the Very Long Instruction Word (VLIW) and the vector processing paradigms together with other ways of exploiting coarser grain parallelism, such as Chip MultiProcessing (CMP). As part of this thesis, we analyze the problem of memory disambiguation in multimedia applications, as it represents a serious restriction for exploiting Instruction Level Parallelism (ILP) in VLIW architectures. We state that the real handicap for memory disambiguation in multimedia is the extensive use of pointers and indirect references usually found in those codes, together with the limited static information available to the compiler on certain occasions. Based on the observation that the input and output multimedia streams are commonly disjointed memory regions, we propose and implement a memory disambiguation technique that dynamically analyzes the region domain of every load and store before entering a loop, evaluates whether or not the full loop is disambiguated and executes the corresponding loop version. This mechanism does not require any additional hardware or instructions and has negligible effects over compilation time and code size. The performance achieved is comparable to that of advanced interprocedural pointer analysis techniques, with considerably less software complexity. We also demonstrate that both techniques can be combined to improve performance.In order to deal with the inherent Data Level Parallelism (DLP) of multimedia kernels without disrupting the existing core designs, major processor manufacturers have chosen to include MMX-like µSIMD extensions. By analyzing the scalability of the DLP and non-DLP regions of code separately in VLIW processors with µSIMD extensions, we observe that the performance of the overall application is dominated by the performance of the non-DLP regions, which in fact exhibit only modest amounts of ILP. As a result, the performance achieved by very wide issue configurations does not compensate for the related cost. To exploit the DLP of the vector regions in a more efficient way, we propose enhancing the µSIMD -VLIW core with conventional vector processing capabilities. The combination of conventional and sub-word level vector processing results in a 2-dimensional extension that combines the best of each one, including a reduction in the number of operations, lower fetch bandwidth requirements, simplicity of the control unit, power efficiency, scalability, and support for multimedia specific features such as saturation or reduction. This enhancement has a minimal impact on the VLIW core and reaches more parallelism than wider issue µSIMD implementations at a lower cost. Similar proposals have been successfully evaluated for superscalar cores. In this thesis, we demonstrate that 2-dimensional Vector-µSIMD extensions are also effective with static scheduling, allowing for high-performance cost-effective implementations.

Context. Data leakage prevention (DLP), a system designed to prevent leakage and loss of secret sensitive data and at the same time not affect employees workflow. The aim is to have a system covering every possible leakage point that exist. Even if these are covered, there are ways of hiding information such as obfuscating a zip archive within an image file, detecting this hidden information and preventing it from leaking is a difficult task. Companies pay a great deal for these solutions and yet, as we uncover, the information is not safe. Objectives. In this thesis we evaluate four different existing types of DLP systems out on the market today, disclosing their weaknesses and found ways of improving their security. Methods. The four DLP systems tested in this study cover agentless, agent based, hybrids and regular expression DLP tools. The test cases simulate potential leakage points via every day used file transfer applications and media such as USB, Skype, email, etc. Results. We present a hypothetical solution in order to amend these weaknesses and to improve the efficiency of DLP systems today. In addition to these evaluations and experiments, a complementing proof of concept solution has been developed that can be integrated with other DLP solutions. Conclusions. We conclude that the exisiting DLP systems are still in need of improvement, none of the tested DLP solutions fully covered the possible leakage points that could exist in the corporate world. There is a need for continued evaluation of DLP systems, aspects and leakage points not covered in this thesis as well as a follow up on our suggested solution.

This diploma thesis deals with a new concept of maintenance and operation of production technologies, where spare parts are manufactured using rapid prototyping methods. The options are compared with currently used methods. Furthermore, methodological recommendations are written on how to proceed with the application. The aim of the work is to evaluate the concept and confirm the theoretical assumptions on a case study.

This diploma thesis deals with the issue of fast high-quality prototype production of printed circuit boards for the needs of the mechatronics lab. Within the scope of this thesis a prototype machine was proposed and created together with control software, which by the help of DLP projector and two stage axis allows to produce printed circuit board in relatively short period of time.

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A programmable illuminating system (PIS) uses a commercial multimedia projector together with a suitable optical relay system in order to illuminate specimens under microscope with transmitted light. The theoretical part of the diploma thesis describes some methods used in the optical transmission microscopy. All of these methods employ physical masks placed in the condenser front focal plane. In the case of the traditional methods the masks are used to enhance contrast (e.g. dark-field illumination) and resolution (oblique illumination). One of the methods (a condenser with rotating aperture) provides the information about the three-dimensionality of the specimen. The next part of the thesis contains the theory and the basic classification of the illuminating systems used in the optical transmission microscopy. An optical and mechanical design of the optical relay system used for PIS is introduced. The experimental part shows the results made with two different PIS arrangements. It was shown on two different specimens that the PIS provides the same illumination as the classical methods. It was also proved that the PIS can simulate the rotating aperture in the condenser front focal plane and thus to give the information about the three-dimensionality of the specimen. Some new static and dynamic illuminating methods were introduced.