Thesis (doctoral)--Universität Bern.

Thesis (doctoral)--Washington College of Law. / Typescript. Reproduction of original from Harvard Law School Library.

Thesis (doctoral), Rijks-Universiteit te Groningen, 1948. / "Literatuurlijst": p. 261-264.

Domain swapping is a mechanism of protein oligomerization whereby subunits exchange identical structural elements with each other. This type of interaction is important in a number of biologically important processes, including the regulation of enzyme activity, the modulation of molecular recognition and in the development of some protein deposition diseases. Domain swapping can be promoted by exposure to chemical denaturants, high temperatures, or redox reagents, although these factors can deviate greatly from conditions encountered in vivo. Moreover, in some cases these conditions can alter the monomer-oligomer equilibrium or even promote the formation of alternate domain-swapped oligomers. In contrast, we have found that it is possible for detergent micelles to be used as a catalyst in the domain-swapping interaction involving the N-terminal cytoplasmic domain of E. coli GlpG rhomboid protease. Our results show that hexadecylphosphocholine micelles can catalyze domain swapping of NGlpG by lowering the Gibbs free energy of the kinetic barrier by 12 kcal/mol, while preserving the equilibrium populations of monomer and dimer. Micelle charge and size were found to be important for this catalysis, which involved the formation of a partially unfolded micelle-bound intermediate containing significant secondary structure. Overall, the results from this work reveal how detergent micelles can affect the energy landscape of domain swapping for NGlpG, and provide insight into the potential ability of local irregularities in lipid membrane environments to play a role in domain swapping in vivo.

archives@tulane.edu / 1 / Md Fakar Uddin

Model Driven software development has been considered to be a further software construction technology following object-oriented software development methods and with the potential to bring new breakthroughs in the research of software development. With deepening research, a growing number of Model Driven software development methods have been proposed. The model is now widely used in all aspects of software development. One key element determining progress in Model Driven software development research is how to better express and describe the models required for various software components. From a study of current Model Driven development technologies and methods, Domain-Specific Modelling is suggested in the thesis as a Model Driven method to better realise the potential of Model-Driven Software Development. Domain-specific modelling methods can be successfully applied to actual software development projects, which need a flexible and easy to extend, meta-modelling language to provide support. There is a particular requirement for modelling languages based on domain-specific modelling methods in Meta-modelling as most general modelling languages are not suitable. The thesis focuses on implementation of domain-specific modelling methods. The "domain" is stressed as a keystone of software design and development and this is what most differentiates the approach from general software development process and methods. Concerning the design of meta-modelling languages, the meta-modelling language based on XML is defined including its abstract syntax, concrete syntax and semantics. It can support description and construction of the domain meta-model and the domain application model. It can effectively realise visual descriptions, domain objects descriptions, relationships descriptions and rules relationships of domain model. In the area of supporting tools, a meta-meta model is given. The meta-meta model provides a group of general basic component meta-model elements together with the relationships between elements for the construction of the domain meta-model. It can support multi-view, multi-level description of the domain model. Developers or domain experts can complete the design and construction of the domain-specific meta-model and the domain application model in the integrated modelling environment. The thesis has laid the foundation necessary for research in descriptive languages through further study in key technologies of meta-modelling languages based on Model Driven development.

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