No description available.

No description available.

No description available.

Many barriers can limit restoration success. In the first chapter of this thesis, the barrier of strong seed dormancy is addressed. While dormancy benefits the species' long-term survival, it can present a challenge within a restoration scenario where rapid establishment is required. Soaking seeds in gibberellic acid (GA3) can overcome dormancy. An easier and potentially more effective method to apply this hormone is to coat seeds with a GA3-impregnated polymer, which provides a slow release of the hormone. Seed dormancy can also be mitigated by creating a favorable microsite with increased soil moisture. We compared the emergence and establishment of penstemon seeds that were coated with GA3 to uncoated seeds planted in shallow drill rows versus deep, U-shaped furrows. These treatments were evaluated in fall and spring plantings at three field sites in the Great Basin Region of the United States. Overall, coating with GA3 improved the emergence and establishment of Palmer's penstemon (Penstemon palmeri; p < 0.01) and thickleaf penstemon (P. pachyphyllus; p < 0.001) but did not improve the emergence or establishment of firecracker penstemon (P. eatonii; p = 1). Between planting seasons, fewer seedlings emerged or established from spring than from fall planting (p < 0.001). Emergence and establishment were higher for all species in deep furrows than in shallow drill rows (p < 0.001). These results indicate that GA3 seed coating and deep, U-shaped furrows may improve the restoration success of some native forbs. Land managers could use these techniques to restore native forbs in dry, disturbed areas. The second chapter of this thesis addresses another barrier to successful restoration, specifically pathogenesis from soil and seed-borne fungus. Survival and growth of native seeds and seedlings can be limited by soil and seed-borne pathogens. Fungicides can combat fungal pathogens, but in some studies, fungicide treatments were ineffective at improving seedling emergence. These studies cite dry conditions leading to low fungal presence as the cause of the ineffectiveness of fungicide treatments for some years and sites. This study tested if fungicide treatment effectiveness is indeed related to the amount of fungus in the soil. We analyzed the emergence and biomass of uncoated, blank-coated, and fungicide-coated bluebunch wheatgrass (Pseudoroegneria spicata) across five soil fungal levels. For both percent emergence and total biomass, uncoated seed performed best in autoclaved soil and declined with increasing level of fungus, but the level of fungus did not impact fungicide-coated seed. When grown in autoclaved, untreated, or low fungal soils, percent emergence and total biomass from fungicide-coated seeds was not different from uncoated seeds. However, in medium and high fungal soils, the percent emergence and total biomass from fungicide-coated seeds were more than two times greater than uncoated seeds (p < 0.05). These results indicate fungicide seed coatings can be effective at increasing restoration success for bluebunch wheatgrass, but the effectiveness of this treatment depends on the microbial environment of the planting site.

Bud dormancy is an essential characteristic of deciduous woody perennials, including apple, to cope with the low temperatures during winter. The release from dormancy and subsequent budburst in apple can only occur after fulfillment of chilling and heat requirements. In the Mid-Atlantic region, dormancy release and bud break of apple often coincide with late-spring freezes that cause severe damages to flowers, and small fruitlets. Therefore, the present study aimed to better understand mechanisms underlying bud dormancy in apple, with an ultimate goal of exploring chemical and/or genetic approaches for bloom-time modulation to avoid spring frost. Using two apple cultivars, 'Cripps Pink' and 'Honeycrisp,' representing early- and late-blooming cultivars, respectively, the present study specifically investigated the accumulation kinetics of plant hormones, carbohydrates, and reactive oxygen species (ROS) throughout the dormancy-regrowth cycle. Our results indicated that both cultivars required 1000 chilling hours for endodormancy release, but 'Honeycrisp' required 1000 growing degree hours (GDHs) more than 'Cripps Pink' for ecodormancy release and budburst. Among plant hormones, abscisic acid (ABA) showed remarkably elevated levels in the dormant buds of both cultivars during endodormancy, but its levels were significantly higher in 'Honeycrisp'. The decline of the ABA level at bud burst was combined with increased levels of cytokinin (CK). The ABA accumulation pattern during dormancy paralleled with an upregulation and downregulation of ABA biosynthetic and catabolic genes, respectively. On the other hand, the levels of hydrogen peroxide (H2O2) and superoxide (O2.-) were significantly higher in 'Cripps Pink' than 'Honeycrisp', particularly by the time of endodormancy and ecodormancy release, respectively. Our findings also showed a gradual decline in starch levels with the dormancy progression and increased levels of total soluble sugars (TSS) that were generally higher in the early-blooming cultivars. Transcriptomic profiling and module-trait relationship identified two modules that contrast between two cultivars mainly during eco-dormancy. Gene ontology (GO) analysis indicated that these DEGs were mostly involved in pathways related to hormones and signaling and co-expressed with H2O2 whereas, during ecodormancy pathways related to glutathione metabolism, auxin biosynthesis, carbohydrate metabolism and reproductive development were co-expressed with O2.-. Together, our results suggest that the contrasting bloom dates between 'Cripps Pink' and 'Honeycrisp' can be explained, at least partially, by the differential accumulation levels of ABA, ROS, antioxidants, and their associated genes in the buds of these cultivars throughout the dormancy cycle. / Doctor of Philosophy / Spring frosts represent a significant threat to apple production in many fruit-producing states of the United States including Virginia. The risk of frost damage is rising due to global climate change, and there is a high demand for effective measures to reduce frost damage. Exogenous applications of plant growth regulators (PGRs) to delay bloom has been suggested as an effective frost avoidance strategy, but with limited success. Therefore, the present study aimed to investigate molecular and biochemical pathways regulating bud dormancy and bloom time in apple, which can ultimate lead to novel approaches for bloom delay and frost mitigation. To this end, the accumulation patterns of major plant hormones (e.g. abscisic acid, ABA, cytokinin, CK and jasmonic acid, JA), reactive oxygen species (ROS) and carbohydrates (e.g. starch, sucrose, glucose and fructose) were thoroughly monitored throughout the dormancy-regrowth cycle in two apple cultivars, 'Cripps Pink' and 'Honeycrisp,' representing early- and late-blooming cultivars, respectively. Both these cultivars had similar chilling requirements (1000 chilling hours) but differed in their heat requirements; with 'Honeycrisp' requiring 1000 growing degree hours more than 'Cripps Pink'. Among plant hormones, ABA increased with the progression of dormancy and decreased with dormancy release in both cultivars. However, ABA levels were significantly higher in 'Honeycrisp' compared to 'Cripps Pink'. On the contrary, during dormancy release, the growth-promoting hormone, CK, increased earlier in 'Cripps Pink'. The levels of ROS, e.g., hydrogen peroxide (H2O2), and superoxide (O2.-), were also higher in 'Cripps Pink' than 'Honeycrisp', particularly by the time of endodormancy and ecodormancy release, respectively. Our data showed that starch levels generally declined during dormancy, whereas soluble sugars increased. However, there was no significant alternations in the carbohydrate accumulation profiles between the two cultivars that could account for the differences in their bloom dates. These results were verified further at the transcriptomic level. Using the RNA-sequencing technology, identified two modules that contrast between two cultivars mainly during eco-dormancy. Gene ontology (GO) analysis indicated that these genes were mostly involved in pathways related to hormones and signaling and co-expressed with H2O2 whereas during ecodormancy pathways related to glutathione metabolism, auxin biosynthesis, carbohydrate metabolism and reproductive development were co-expressed with O2.-. Overall, our results suggest that ABA, cytokinin, H2O2, and O2.- may, at least partially, explain the differences in the bloom time between the two apple cultivars. Further analysis of these molecules and their associated genes in other apple cultivars with contrasting bloom dates is necessary for better understanding of bloom time regulation in apple and developing strategies against frost damage.

Organisms are often adapted to their local environment, but the role of early life stages in adaptive differentiation among populations remains poorly known. The aim of my thesis was to investigate the contribution of early life stages to the magnitude and genetic basis of local adaptation, and to identify the underlying adaptive traits. For this, I used two natural populations of the annual plant Arabidopsis thaliana from Italy and Sweden, and a Recombinant Inbred Line (RIL) population derived from a cross between these populations. By combining greenhouse and field experiments, Quantitative Trait Loci (QTL) mapping, and path analysis, I examined (1) the genetic basis of seed dormancy, (2) the contribution of differential seedling establishment to local adaptation, (3) among-year variation in selection during seedling establishment, (4) direct and indirect effects of seed dormancy and timing of germination on fitness, and (5) the adaptive value of the seed bank. I found that both the level and the genetic basis of seed dormancy were affected by the maternal environment. One major-effect QTL was identified in all maternal environments, which overlaps with the dormancy gene DELAY OF GERMINATION 1 (DOG1). Selection through seedling establishment success contributed strongly to local adaptation and genetic tradeoffs, and varied among years. Variation in seedling establishment and overall fitness among RILs could be explained by genetically based differences in seed dormancy and timing of germination. Seed dormancy affected fitness throughout the life cycle, by affecting the proportion of germinated seeds, and indirectly via effects on timing of germination, plant size and flowering time. My results suggest that a considerable portion of A. thaliana seeds enter the seed bank. I found genetic differences in dormancy cycling behaviour between the two populations, which could contribute to local adaptation. The value of a seed bank should be higher at the Swedish study site than at the Italian study site due to lower rate of seed mortality in the soil. Overall, the results of this thesis demonstrate that early life stages contribute strongly to both the magnitude and the genetics of local adaptation.

The release of genetically modified, herbicide tolerant canola (<I>Brassica napus</i> L.) genotypes in western Canada has increased interest in the persistence of volunteer canola. <i>B. napus</i> seed may be induced into secondary dormancy in the laboratory, however, little is known of the seedbank ecology and the role of secondary dormancy as a persistence mechanism in this species in the region. The objectives of this research were i) to determine seedbank additions at the time of harvest, ii) determine the role of secondary seed dormancy in seedbank persistence under different management systems, iii) determine the relative importance of factors contributing to secondary dormancy potential and iv) evaluate the role of abscisic acid (ABA) during secondary dormancy induction in <I>B. napus</i>. On farms, average seedbank additions during harvest were approximately 20 times the normal seeding rate of canola. High secondary seed dormancy potential prolonged seedbank persistence in fields, irrespective of tillage system. <I>B. napus</i> exhibited seedling recruitment of a typical summer annual weed where seedling recruitment was only observed in the spring. Seasonal seedling recruitment was the result of two fates: seed death in the shallow seedbank, irrespective of dormancy potential, and increased ungerminability in buried seeds which was related to secondary seed dormancy potential. Among the factors that contribute to secondary seed dormancy potential, genotype was of greatest significance. Seed size was of lesser importance, while the contributions of pre-harvest factors including seed maturity, year, and location were negligible in comparison. Differences in ABA synthesis and the response to ABA application were related to secondary seed dormancy potential and correlated well previous reports linking ABA to seed dormancy. Conclusions that emerge from this research are i) that on some farms, seedbank additions may be lowered by more diligent harvest practices, ii) seedbank persistence of <I>B. napus</i> may be reduced by growing low dormancy genotypes and avoiding seed burial for one year after seedbank establishment and iii) ABA + ABA-glucose ester (ABA-GE) and the ability of seeds to respond to ABA application after seed dormancy induction may potentially be used to identify seed dormancy potential in this species.

The release of genetically modified, herbicide tolerant canola (<I>Brassica napus</i> L.) genotypes in western Canada has increased interest in the persistence of volunteer canola. <i>B. napus</i> seed may be induced into secondary dormancy in the laboratory, however, little is known of the seedbank ecology and the role of secondary dormancy as a persistence mechanism in this species in the region. The objectives of this research were i) to determine seedbank additions at the time of harvest, ii) determine the role of secondary seed dormancy in seedbank persistence under different management systems, iii) determine the relative importance of factors contributing to secondary dormancy potential and iv) evaluate the role of abscisic acid (ABA) during secondary dormancy induction in <I>B. napus</i>. On farms, average seedbank additions during harvest were approximately 20 times the normal seeding rate of canola. High secondary seed dormancy potential prolonged seedbank persistence in fields, irrespective of tillage system. <I>B. napus</i> exhibited seedling recruitment of a typical summer annual weed where seedling recruitment was only observed in the spring. Seasonal seedling recruitment was the result of two fates: seed death in the shallow seedbank, irrespective of dormancy potential, and increased ungerminability in buried seeds which was related to secondary seed dormancy potential. Among the factors that contribute to secondary seed dormancy potential, genotype was of greatest significance. Seed size was of lesser importance, while the contributions of pre-harvest factors including seed maturity, year, and location were negligible in comparison. Differences in ABA synthesis and the response to ABA application were related to secondary seed dormancy potential and correlated well previous reports linking ABA to seed dormancy. Conclusions that emerge from this research are i) that on some farms, seedbank additions may be lowered by more diligent harvest practices, ii) seedbank persistence of <I>B. napus</i> may be reduced by growing low dormancy genotypes and avoiding seed burial for one year after seedbank establishment and iii) ABA + ABA-glucose ester (ABA-GE) and the ability of seeds to respond to ABA application after seed dormancy induction may potentially be used to identify seed dormancy potential in this species.

Penstemon gibbensii and Penstemon haydenii are two rare, perennial forbs inhabiting remote areas of the western United States. P. gibbensii is listed as a sensitive species by the Bureau of Land Management (BLM) in Colorado, Utah, and Wyoming (Heidel, 2009). P. haydenii was designated as Endangered by the U.S. Fish and Wildlife Service in 1987 (Heidel, 2012). This thesis research was geared toward helping land managers in their efforts to protect and rehabilitate these species by providing understanding on different aspects of their seed ecology. My first study was a laboratory experiment performed on P. gibbensii and P. haydenii seed germination response to moist chilling and dry after-ripening. Wild harvested seeds were subjected to moist chilling at 2-4 °C for 0, 4, 8, 12, and 16 weeks and held in dry storage for approximately 2 years to determine effective methods for breaking primary dormancy. P. gibbensii seed germination increased consistently with increased length of chilling up to 16 weeks and exhibited habitat-correlated variation in this response. P. haydenii seed germination increased from 1 to 100% germination with 4 weeks of chilling. P. haydenii germination was greatest (96%) when incubated under a cool, diurnally-fluctuating temperature regime (10-20 °C) and responded positively to dry storage, increasing germination from 0 to 15%. My second study was an in situ field study designed to characterize the active seed bank of P. haydenii. We set up a transect line across a P. haydenii population and measured the number of seeds entering the seed bank, lost to predation post-dispersal, and persisting in the seed bank. P. haydenii does not appear to form an ecologically significant seed bank. Approximately 140 seeds/ 10m2 could potentially enter the seed bank but only 1 seed in the upper 10cm of sand persisted. Heavy post-dispersal insect predation resulted in a decrease in viability of nearly 30% in exposed P. haydenii seeds after just 12 hours. My third study explored the effects of burial by sand on P. haydenii. Wild-harvested seeds were planted in pots at 1, 2, 4, 6, 8, and 10cm deep in sand and incubated at 10-20 °C. Seed germination and mortality and seedling emergence were measured. The response of dormant seeds to post-burial incubation was determined. Burial depth decreased seedling emergence and seed germination. Shallow burial appears to induce secondary dormancy for seeds that don't germinate quickly, whereas deep burial appears to impose enforced dormancy in burial.

No description available.