Diplomová práce se zabývá problematikou strategického měření výkonnosti podniků. Konkrétně je zacílena na měření výkonnosti potravinářské firmy působící na trhu výroby a prodeje surovin pro pekaře, cukráře a gastronomii. Řeší především důležitost sledování výkonnosti podniku, ukazuje možné moderní koncepty měření výkonnosti, zabývá se metrikami a odhaluje podstatu multidimenzionálního přístupu. Za pomoci získaných teoretických znalostí je následně vytvořen model metrik potravinářské firmy. Pro ověření možností sledovat vybrané metriky vytvořeného modelu je použit nástroj Microsoft Analysis Services, který je jedním z typických představitelů systémů Business Intelligence. Diplomová práce má dva hlavní výstupy. Prvním je model metrik potravinářské firmy ve formě strategické mapy, druhým jsou grafické reporty sledující vybrané metriky v nástroji MS Excel.

This thesis deals with the aggregation behaviour of palmitoylhyaluronan in two degrees of substitution, namely 10 % and 16 %. Using a fluorescence spectroscopy method with pyrene as a fluorescence probe, we found a critical micellar concentration. The results were confirmed by measuring the dynamic light scattering, which also showed an increase in the size of aggre-gates with an increasing concentration. System stability is reduced by increased ionic strength as evidenced by zeta potential measurement. Experiments found that 16 % substitution sample can form a gel. The gel is very stiff and has excellent properties, which was confirmed by rhe-ology. We also managed to incorporate pyrene in the gel, which was demonstrated by the presence of highly solvated domains that could be polymer micelles. Based on these results, 16% substitution sample was subjected the MTT assay to cytotoxicity. The results confirmed that the examined sample was not toxic.

The diploma thesis deals with nonlinear dynamical systems with emphasis on typical phenomena like bifurcation or chaotic behavior. The basic theoretical knowledge is applied to analysis of selected (chaotic) models, namely, Lorenz, Rössler and Chen system. The practical part of the work is then focused on a numerical simulation to confirm the correctness of the theoretical results. In particular, an algorithm for calculating the largest Lyapunov exponent is created (under the MATLAB environment). It represents the main tool for indicating chaos in a system.

The master thesis deals with the possibility of using dynamic damper in hydrodynamics. Specifically, the assessment of the impact of dynamic damper on the dynamic stability of the fluid system when the system exhibited unstable behaviour prior to damper installation due to self-excited vibration. In the thesis is presented the algorithm for the calculation of pressure and flow pulsations based on the transfer matrix method, the transition matrices for different damper designs variants are derived. Using the algorithm, the effect of a damper placed in a stable and unstable fluid system is solved. The output of this work is software for solution of pressure and flow pulsations in the system with serially arranged hydrodynamic elements created in the MATLAB program.

For the supersonic flow mode, which occurs in the internal flow behind the aperture separating two spaces with a large pressure drop, the critical flow is a characteristic phenomenon. In the case of critical flow behind the aperture, a supersonic flow area with reduced pressure ending with a shock wave with a step change in state variables is created. When diagnosing velocities, which are obtained from the values of static and total pressure, due to the changes caused by the shock wave passage, correction of the diagnostic quantities obtained by measuring by means of mathematical relations taking into account the physical phenomena is necessary.

This thesis is based on the series of scholarly article dedicated to the issue ofpumping in the differential scanning chamber of an environmental scanning microscope. The thesis is based on Danilatos’s study where the pumping of the differential pumped chamber is solved by means of the Monte Carlo statistical method. The thesis performs analysis of gas flow at low pressures through orifice separating the spaces with a large pressure drop Dr. Danilate. The analyses will be used for the design of the experimental chamber which will serve for the experimental evaluation of the flow results in the chamber using the continuum mechanics.

The aim of this thesis was to prepare hyaluronic acid micro- and nanoparticles based on electrostatic interactions with oppositely charged molecules. Following parameters were monitored: correlation function behavior, the particle size and zeta potential value. At the beginning, it was necessary to study the behavior of hyaluronan in solution by dynamic light scattering measurement. Micro- and nanoparticles were prepared by mixing different volume ratios of negatively charged hyaluronan and positively charged polyarginine or cetyltrimethylammonium bromide. Micro- and nanoparticles were prepared in aqueous solution as well as in 0,15 M sodium chloride solution (physiological solution). In the case of the hyaluronan solution a polydisperse character of hyaluronan was detected. It was found that the dissolution of hyaluronan in the physiological solution gives us the smaller particle size in opposite to particle size obtained from the same concentrations of hyaluronan dissolved in water. Furthermore, it was found that systems composed of hyaluronan and polyarginine create particle size of about 100 nm. Whereas systems consisting of cetyltrimethylaminoum bromide and hyaluronan form larger particles, in units of hundreds of nanometers, the particle size in physiological solution were smaller than the same systems dissolved in aqueous solution.

This thesis is focused on investigation of phospholipid-hyaluronan system. First, appropriate method for preparation of bulk solution of phospholipid/lipid and suitable fluorescence probe were chosen. Sonification was selected as a method for preparation of bulk solution and pyrene was chosen as a fluorescence probe. From the group of phospholipids lecithin was selected. Next to phospholipid, lipid with no phosphate group (DPTAP) was utilized for comparison, alternatively a mixture of lipid (DPTAP) and phospholipid (DPPC). Instead of hyaluronan another polyelectrolytes (sodium polystyrene sulfonate, sodium alginate) were used too. Measurements were performed in water environment and in phosphate buffer saline (PBS). All investigation was accomplished by fluorescence spectroscopy and dynamic light scattering.

The effect of hydrophobically modified hyaluronan on surfactants aggregation has been studied in this master’s thesis. The value of critical micelle concentration of anionic surfactant SDBS (sodium dodecylbenzensulfonate), cationic surfactant CTAB (cetyltrimethylamonnium bromide) and nonionic surfactant Triton X-100 (octylphenol ethoxylate) was determined by fluorescence spectroscopy using pyrene probe. Aggregation behavior of surfactants was performed with addition of hydrophobically modified hyaluronan of two molecular weights (17 kDa, 206 kDa) in aqueous solution. The greatest influence of hydrophobized hyaluronan on aggregation behavior was observed in system with cationic surfactant CTAB. Stability of system containing cationic surfactant and hydrophobically modified hyaluronan was established through zeta potential. Last part of thesis deals with size determination using dynamic light scattering.

The thesis deals with modelling and control of wheeled mobile robots. The thesis introduces the mathematical models of diferentially driven mobile robot, robot with Ackerman steering and a skid steered robot. The thesis describes open-loop and feedback control. Feedback control is divided into point-to-point control, path following and trajectory tracking.