The objective of this research is developing a new method of design for cold-formed steel framed shear wall sheathed by ¾" thick USG structural panel concrete subfloor using a predictive analytical model and comparing the results obtained from the model with those achieved from real testing to verify the analytical model and predicted lateral load-carrying capacity resulted from that. Moreover, investigating the impact of various screw spacings on shear wall design parameter such as ultimate strength, yield strength, elastic stiffness, ductility ratio and amount of energy dissipation is another purpose of this research.

Memoria para optar al título de Ingeniero Civil Mecánico / En el desarrollo de las industrias de manufactura se busca acelerar los procesos, en este punto la manufactura aditiva ha ayudado al progreso de la industria gracias a la creación de prototipos rápidos, con precisión, y disminución de costos, entre otros. Existen distintas formas de manufactura aditiva y aplicaciones con distintos materiales, plásticos, metales y cerámicos. Dentro de la manufactura aditiva se encuentran los procesos de proyección térmica usada típicamente con metales, donde se proyectan partículas fundidas, semifundidas o en frío. Este último es el caso de los sistemas de Cold Spray que son utilizados hoy en día para la manufactura de revestimientos, reparación de piezas mecánicas, relleno de orificios, relleno de piezas de fundición defectuosas, entre otros tipos de aplicaciones. Lo mencionado anteriormente tiene aplicaciones en el área de energía, aeroespacial, militar, automotriz, entre otros. En este proceso de manufactura se proyectan partículas metálicas, por intermedio de un gas que es calentado y acelerado mediante una tobera de Laval a velocidades supersónicas, las partículas logran su adhesión por la deformación plástica sufrida en el impacto. La presente Memoria consiste el diseño, construcción y pruebas de un sistema de Cold Spray de baja presión. Este proyecto se divide en varias etapas: Definir criterios de dise- ño, Diseño preliminar, Memoria de cálculo, Selección de componentes, Diseño y validación mediante elementos finitos, Construcción y ensamblaje, Pruebas. Luego de fabricado el sistema se realizan una serie de pruebas en cada uno de sus subsis- temas (control gas, control temperatura, movimiento y gabinete protección). Estas pruebas sirven para observar desperfectos, analizar sus posibles causas y opciones de solución. Una vez terminada la construcción y pruebas, se plantean mejoras a futuro, donde se destaca la construcción de una nueva tobera y un alimentador de partículas; así como también, la aislación de las partes mecánicas (husillos y ejes lineales), ya que éstas pueden sufrir daños o desperfectos por suciedad o polvos proyectados. Finalmente con el sistema de Cold Spray se busca realizar posteriores estudios en el ámbito de la manufactura aditiva y así conocer distintos tipos de aplicaciones según sus propiedades mecánicas.

Web crippling is a form of localized buckling that occurs at points of transverse concentrated loading or supports of thin-walled structural members. The theoretical computation of web crippling strength is quite complex as it involves a large number of factors such as initial imperfections, local yielding at load application and instability of web. The existing design provision in North American specification for cold-formed steel C-sections (AISI S100, 2007) to calculate the web-crippling strength is based on the experimental investigation. The objective of this research is to extend the direct strength method to the web crippling strength of cold-formed steel C-sections. ABAQUS is used as a main tool to apply finite element analysis and is used to do the elastic buckling analysis. The work was carried out on C-sections under interior two flange (ITF) loading, end two flange (ETF) loading cases. Total of 128 (58 ITF, 70 ETF) sections were analyzed. Sections with various heights (3.5 in.to 6 in.) and various lengths (21 in. to 36 in.) were considered. Data is collected from the tests conducted in laboratory and the data from the previous researches is used, to extend the direct strength method to cold formed steel sections. Proposing a new design for both the loading cases and calculation of the resistance factors under (AISI S100, 2007) standards is done.

The cold-formed steel framed shear walls with steel sheets and wood-based sheathing are both code approved lateral force resisting system in light-framed construction. In the United States, the current design approach for cold-formed steel shear walls is capacity-based and developed from full-scale tests. The available design provisions provide nominal shear strength for only limited wall configurations. This research focused on the development of analytical models of cold-formed steel framed shear walls with steel sheet and wood-based sheathing to predict the nominal shear strength of the walls at their ultimate capacity level. Effective strip model was developed to predict the nominal shear strength of cold-formed steel framed steel sheet shear walls. The proposed design approach is based on a tension field action of the sheathing, shear capacity of sheathing-to-framing fastener connections, fastener spacing, wall aspect ratio, and material properties. A total of 142 full scale test data was used to verify the proposed design method and the supporting design equations. The proposed design approach shows consistent agreement with the test results and the AISI published nominal strength values. Simplified nominal strength model was developed to predict the nominal shear strength of cold-formed steel framed wood-based panel shear walls. The nominal shear strength is determined based on the shear capacity of individual sheathing-to-framing connections, wall height, and locations of sheathing-to-framing fasteners. The proposed design approach shows a good agreement with 179 full scale shear wall test data. This analytical method requires some efforts in testing of sheathing-to-framing connections to determine their ultimate shear capacity. However, if appropriate sheathing-to-framing connection capacities are provided, the proposed design method provides designers with an analytical tool to determine the nominal strength of the shear walls without conducting full-scale tests.

A prototype rigid wall relocatable shelter was designed and constructed using cold-formed steel (CFS) construction techniques including shear walls with corrugated sheathings. The design of the shelter was to be mechanically sound with adequate thermal performance and resistance to corrosion. Modeling of structural shear walls was performed using ABAQUS and verified with experimental results. At the project's conclusion, a completed full-scale prototype shelter was constructed.

Recrystallisation annealing, a repeated heat treatment between different stages of cold rolling of martensitic chromium steel strip, is successful when neither high rolling forces nor wear of the working rolls occur during the subsequent cold rolling. Mechanical properties as tensile strength, yield, elongation or hardness have been, by tradition, the criteria that described the quality of the annealing process. In recent years, the development of the measurement equipment in the rolling mills and of the instruments for material investigations has accentuated more and more the role played by the microstructural properties in the evaluation of the heat treatment. Two microstructural characteristics of the degree of annealing are, firstly and most important, the recrystallisation degree, and, secondly, the secondary carbide density. The sample manufacturing and heat treatment, modelling and microstructure investigations by light optical- (LOM) and scanning electron microscopy (SEM) described in this article were carried out at Sandvik Materials Technology’s R&D Department and Bell Furnace Line in Sandviken, Sweden, while microstructure investigations and evaluation by scanning electron microscopy with field emission gun (FEG-SEM) and electron back scatter diffraction (EBSD) were done at the Corrosion and Metals Research Institute (KIMAB) in Stockholm, Sweden. The first part of this work shows that, in contrast to the traditional methods LOM and SEM, that use chemical etching for the preparation of the samples, EBSD can successfully characterise recrystallised structures in annealed martensitic chromium steels. Unlike conventional microscopy with LOM and SEM, EBSD is able to reveal the grain geometry, as well as to separate and identify the different phases in this kind of steels (ferrite, M23-, M6-carbides). Important parameters such as grain size, particle size and recrystallised fraction can be measured with high accuracy. This information can be used to understand, evaluate, control and even predict the recrystallisation annealing of martensitic chromium steel. The second part of this work presents how the results from microstructure description by EBSD can be directly used in relatively simple empirical models for determination of recrystallisation degree as function of the annealing parameters and the deformation history. EBSD was applied to evaluate the degree of recrystallisation in a series of annealing tests, with the purpose to model recrystallisation temperature in two types of martensitic chromium steel strip, a traditional one and one alloyed with molybdenum, cold rolled with different amounts of reduction and annealed with different temperatures, soaking times and heating rates. The empirical quadratic models were built with Umetrics’ software for experimental design, MODDEÒ 8.0 and they defined the recrystallisation degree (limits for LAGB and HAGB were set to 1.5° and 7.5° for the first grade and 2.5° and 10° for second one) and the secondary carbides density as functions of annealing temperature, soaking time and cold reduction (the factor heating rate was removed as nonsignificant). To be observed that these empirical models were fit much better for the recrystallisation degree than for the secondary carbides density. The modelling work described above, together with the implementation of online physical temperature models in the bell annealers may lead to an increased productivity in the production plant by shortening the annealing cycle and minimising scrap and thus to an economical gain of ca 1,5 MSEK per year at Sandvik Materials Technology.

The purpose of this study is to determine the effect of short-time cold pack cryotherapy in thermoreceptors, therefore, proprioception via deep sensation receptors and sense of balance on ankle joint in healthy adults. In the subject, I refer to other studies using the word proprioception when measuring the cold effect in the balance of the ankle joint. Mainly, cold therapy is affecting to the thermoreceptors but there might be input also to change neuromuscular transmission. The aim of the experimental process is to identify if the cold application on the ankle joint for 20 minutes is decreasing dynamic postural stability and the sense of balance in laboratory environment. Short term cryotherapy effect is measured within 12 participants divided into two groups: control and study group. Both groups went through the first measurement, 20 minutes rest with or without cold application and second measurement directly after the rest period. The dynamic posturography machine SMART Balance Master System (NeuroCom) was used to evaluate somatosensory outcome in the experiment. In the systematic review the previous studies are investigated within same settings as on experimental process, short time cold application on ankle joint measuring the effect on ankle proprioception and joint position sense. The...

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Salmonella is a food-borne pathogen that leads to substantial illness worldwide. The clinical syndromes associated with Salmonella infection are enteric (typhoid) fever and gastroenteritis, in healthy humans. Typhoid fever is caused by host-adapted S. Typhi and S. Paratyphi. Gastroenteritis is caused by serovars usually referred to as non typhoidal Salmonellae (NTS). In recent years, an increasing number of outbreaks due to NTS, despite increased efforts in food safety, were reported because of persistence of Salmonella in the food chain. Thus I hypothesized that Salmonella is able to withstand stresses in the environment and treatments used during food processing for its elimination and thereby able to develop resistance against subsequent stress encounters. The effect of cold, peroxide, and acid was tested on survival of S. Typhimurium and the survival was persistent under cold stress (5°C) for up to 240 h. Pre-adaptation to cold stress (5°C, 5 h) also increased survival of S. Typhimurium during subsequent exposure to acid stress (pH 4.0, 90 min) by repressing hydroxyl radical formation. Cold stress (5°C, 48 h) to S. Typhimurium significantly (p < 0.05) increased its adhesion and invasion in intestinal iv epithelial cells. This phenotype was attributed to a pair of protein-protein interactorsacting as receptors on microbial (STM2699) and host cell surface (SPTAN1). Cold stress significantly (q < 0.05) induced STM2699 in S. Typhimurium and SPTAN1 was significantly (q < 0.05) induced in pithelial cells upon infection with cold-stressed S. Typhimurium. Cold stress to S. Typhimurium also significantly (q < 0.05) induced genes related to virulence such as type 3 secretion system apparatus and effectors genes, prophage genes, and plasmid genes and they remain induced upon infection of epithelial cells with additional induction of spv genes on the plasmid. Infection of epithelial cells with cold-stressed S. Typhimurium significantly (p < 0.05) increased activation of caspase 9 and 3/7. Cold-stressed S. Typhimurium switched metabolism from aerobic respiration to fermentation and it persisted during infection of epithelial cells. As a result, short chain fatty acids formate and acetate, which act as diffusible signal for invasion, were detected in significantly (q < 0.05) high amounts in extracellular media of cells infected with cold-stressed S. Typhimurium supporting the phenotype of high adhesion and invasion of cold-stressed S. Typhimurium in epithelial cells.

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