Tissue optical clearing aims to increase the penetration depth of near-collimated light in biological tissue to enhance optical diagnostic, therapeutic, and cosmetic procedures. Previous studies have shown the effects of chemical optical clearing on tissue optical properties. Drawbacks associated with chemical clearing include the introduction of potentially toxic exogenous chemicals into the tissue, poor site targeting, as well as slow transport of the chemicals through tissue. Thus, alternative clearing methods have been investigated. Mechanical compression is one such alternative tissue optical clearing technique. The mechanisms of action of mechanical compression may be similar to those of chemical clearing, though they have yet to be investigated systematically. This research describes the design and execution of a number of procedures useful for the quantification of the tissue optical clearing effects of localized mechanical compression. The first experimental chapter presents the effects of compression on image resolution and contrast of a target imaged through ex vivo biological tissue. It was found that mechanical optical clearing allowed recovery of smaller targets at higher contrast sensitivity when compared to chemical clearing. Also, thickness-independent tissue clearing effects were observed. In the second experimental chapter, dynamic changes in tissue optical properties, namely scattering and absorption coefficients (?s' and ?a, respectively) were monitored during a controlled compression protocol using different indentation geometries. A reduction in ?s' and ?a was evident for all indentation geometries, with greater changes occurring with smaller surface area. Results indicate that localized mechanical compression may be harnessed as a minimally-invasive tissue optical clearing technique. / Master of Science

The effect of cyclic moisture infiltration on connections in light-frame wood buildings has received limited research attention. Specifically, the connections between wood-based sheathing materials (OSB, plywood) and solid wood studs are of interest. A comprehensive understanding of connection performance will enhance structure and material design, thereby improving the overall integrity and robustness of light-frame structures. The focus of this research project was to evaluate the strength and stiffness of wood-frame connections exposed to cyclic humidity conditioning. Nailed sheathing/stud connection samples were tested for lateral resistance following various periods of moisture exposure. Elastic stiffness, 5% offset yield load, maximum yield load, and failure yield were computed and analyzed using the data collected. The parameters were compared among connection specimens receiving either 0, 1, 5, 10, 15, 25, or 40 periods of cyclic moisture conditioning. In addition, the bearing resistances of the materials were investigated for application to the general dowel equations for calculating lateral connection values, the current basis for design of single dowel-type fastener connections between wood-based members. An x-ray density profilometer was used to observe the de-densification processes within the composite sheathing materials throughout the moisture conditioning regime. Results indicated moderate to extreme changes in the performance of cycled connections involving lower density sheathing materials. Higher density sheathing materials performed favorably at each cycle test period. Comparisons to the yield model were similar to the control results, but usually differed as cycling increased. Analysis of connection performance following cyclic moisture loading is a vital component in developing a holistic model for service-life prediction of nailed connections in light-frame residential construction. / Master of Science

African Americans (AA) are twice as likely as White Americans (WA) to experience diabetes-related foot amputation due to foot ulcers. Foot ulcers are often caused by high plantar pressure, and several factors can impact plantar loading. Thus, there is a need to determine if race is a significant predictor of plantar loading. Additionally, with the current state of racial health disparities there is a need to determine racial differences in research engagement and mistrust between AA and WA. Data was collected from 107 participants, aged 18-30, in this Institutional Review Board approved study. An EMED pressure-measurement system (Novel Electronics, St. Paul, MN, USA) was used to collect plantar loading data. Additional measurements collected from each participant included arch height index (AHI), standing height, gait speed, and weight. Participants also completed two surveys focused on research engagement and research mistrust. A multiple linear regression was used to test if race and other factors significantly predicted plantar loading. Non-parametric tests were used to test if there were significant differences in research engagement and mistrust between AA and WA. The analysis determined that race was a significant predictor for plantar loading, along with age, AHI, gait speed, sex, and body mass index (BMI). Additionally, it was found that research engagement practices and feelings of research mistrust differed significantly between AA and WA young adults. These findings could improve our understanding as to why AA are more likely to have diabetic foot ulcers than WA, and why AA are less likely to participate in research than WA. / M.S. / African Americans (AA) are twice as likely than White Americans (WA) to experience diabetes-related foot amputation due to foot ulcers. Foot ulcers are often caused by high plantar pressure, and several factors can alter plantar loading. Thus, there is a need to determine if race is a significant predictor of plantar loading. Additionally, with the current state of racial health disparities, there is a need to determine racial differences in research engagement and mistrust between AA and WA. Data was collected from 107 participants, aged 18-30. A pressure-measurement system was used to collect plantar loading data in seven regions of the foot during self-selected speed walking. The measurements collected from each participant, included arch height, standing height, gait speed, and weight. Participants were also asked to complete two surveys focused on research engagement and research mistrust. We used this data to evaluate if race and other factors predicted plantar loading and to compare survey responses between AA and WA. It was found that race, age, arch height, gait speed, sex, and BMI were considered significant predictor variables for plantar loading measures. Additionally, research engagement practices and feelings of research mistrust differed significantly between this younger sample of AA and WA. These findings help to improve our understanding of why AA are more likely to have diabetic foot ulcers than WA, and why AA are less likely to participate in research than WA, even as young adults.

Turfgrass management activities on golf courses have been identified as a possible source of Chesapeake Bay nutrient pollution. Total Maximum Daily Load goals are in place to reduce nutrient amounts entering the Bay. Dissertation investigations include (1) the role of golf course turfgrass management in nutrient deposition or attenuation in local streams, (2) estimations of total nitrogen (N) discharging to the watershed from stream outlet points as a function of land use and watershed area, and (3) other factors potentially affecting water quality on golf courses, including soil characteristics and use of best management practices (BMPs). Total N, nitrate-N, ammonium-N, phosphate-phosphorus (P), streamwater temperature, specific conductance (SpC), pH and dissolved oxygen (DO) were sampled at 12-14 golf course stream sites in the James River and Roanoke River watersheds during baseflow conditions. Discharge was determined at outflow locations. Unit-area loads (UALs) were calculated from monitoring data. These UALs were then compared to UALs from Chesapeake Bay Watershed Model land use acreages and simulated loads for corresponding watershed segments. Virginia golf course superintendents were also surveyed to determine BMP use. No consistent impairment trends were detected for streamwater temperature, SpC, pH, or DO at any of the sites. Outflow NO3-N was below the 10 mg L-1 EPA drinking water standard. However, some sites may be at increased risk for benthic impairment with total N concentrations >2 mg L-1, as suggested by VADEQ. Significant increases in nitrate-N at OUT locations were measured at four sites, whereas decreases were measured at two sites. Ammonium-N significantly decreased at two sites. Golf course N UALs calculated from baseflow monitoring were lower than or similar to UALs estimated for forested areas in the associated watershed segment at seven out of the 12 sites. Golf course UALs ranged from 1.3-87 kg N ha-1 yr-1. Twenty-one of 32 surveyed BMPs had an adoption rate ≥50% among survey respondents. In most cases, presence of golf courses generally does not appear to significantly degrade baseflow water quality of streams in this study. Management level appears to be an influencing factor on water quality and concerns may be heightened in urban areas. / Ph. D.

Reinforced Concrete (RC) structures are common in earthquake-prone areas. During an earthquake, the steel reinforcement is subjected to cyclic strain histories which lead to inelastic response. In the case of rare, strong earthquakes, inelastic buckling and even rupture due to low-cycle fatigue can also occur. The understanding and characterization of the performance of RC structures under earthquake hazards requires the accurate simulation of the inelastic hysteretic behavior of steel reinforcement by means of appropriate constitutive models. Several uniaxial material models have been developed for reinforcing steel. Existing material models sacrifice efficiency for accuracy or vice versa. Conceptually simple and numerically efficient models do not accurately capture the hysteretic response and ignore rupture or buckling. On the other hand, more refined material models are characterized by iterative stress update procedures which can significantly increase the computational cost of an analysis. Additionally, experience suggests that refined models attempting for the effect of inelastic buckling tend to lead to numerical convergence problems in the stress update procedure. The goal of the present study is the formulation and implementation of an accurate and computationally efficient constitutive model for steel reinforcement under cyclic loading. A previously developed model, capable of capturing the inelastic hysteretic response of reinforcing steel in the absence of buckling and rupture, is used as a starting point in this study. The model is enhanced by replacing its original, iterative stress update procedure with an equally accurate, non-iterative one. Additionally, the model is enhanced to capture the effects of inelastic buckling and of rupture. The accuracy of the model and the efficiency of the non-iterative stress update algorithm are demonstrated by means of validation analyses. / Master of Science

The 2010-2011 Canterbury, New Zealand, Earthquake Sequence (CES) resulted in 185 fatalities and approximately $NZ40 billion in damage, much of which was due to liquefaction and related phenomena. As a result, an extensive soil improvement field testing program was initiated and Rammed Aggregate Piers� (RAP) were shown to be a feasible method to mitigate the risk from liquefaction during future events. To better design and more fully assess the efficacy of reinforcement techniques against liquefaction, pre- and post-treatment in-situ test data are compiled, to include results from cone penetration tests (CPT), direct-push crosshole tests, and vibroseis (T-Rex) shaking tests. The data are used to evaluate the capabilities of numerical tools to predict the liquefaction response of unimproved and improved sites. A finite difference (FD) numerical model is developed in a FLAC platform and a coupled analysis using the Finn model with Byrne (1991) formulation is conducted. The FD model calibrated for top-down shakings similar to the vibroseis tests succeeded in qualitatively reproducing the general observed behavior without quantitatively matching the in-situ values for shear strains and excess pore pressure ratios. The introduction of the RAP elements to the FD model reduced the shear strain, but slightly overestimated that reduction. Considering more advanced constitutive models that better simulate the complexity of the soil behavior under dynamic loading would likely increase the accuracy of the predicted response. / MS / During earthquakes, a significant loss of strength in soil can occur. This phenomenon, known as liquefaction, can have a devastating impact on the area affected. The 2010-2011 Canterbury, New Zealand, Earthquake Sequence (CES) resulted in 185 fatalities and approximately $NZ40 billion in damage, much of which was due to liquefaction and related phenomena. Consequently, the New Zealand Earthquake Commission implemented a field testing program in order to investigate the efficiency of ground improvement techniques in reducing soil liquefaction potential. One of the tested techniques was Rammed Aggregate Piers™ (RAP) and was shown to be a feasible method in mitigating the risk from liquefaction during future events. The focus of this study is to develop a numerical model capable of predicting the liquefaction response of unimproved and RAP-improved sites. Pre- and post-treatment test data are therefore compiled and used to calibrate the model. The numerical model calibrated for shakings similar to the on-site tests succeeded in qualitatively, but not quantitatively, reproducing the behavior observed in the field. The introduction of the RAP elements to the model revealed an improvement against liquefaction hazard; however, the improvement was overestimated compared to the field results. Considering more advanced numerical features that better simulate the complexity of the soil behavior under dynamic loading would likely increase the accuracy of the predicted response.

The racking performance of light-frame shear walls subjected to monotonic and cyclic loading is the focus of this thesis. The sheathing materials investigated are oriented strandboard (OSB), hardboard, fiberboard, and gypsum wallboard. The objectives of this study were to (1) obtain and compare performance characteristics of each sheathing material; (2) compare the effects of monotonic loading versus the cyclic loading response; (3) investigate the contribution of gypsum in walls with dissimilar sheathing materials on opposite sides of the wall; and (4) study the effects of using overturning anchors. The monotonic tests, which incorporated the use of hold-downs, were performed according to ASTM E564. Half of the cyclic tests were performed with hold-downs, and half were performed without hold-downs. The cyclic tests were performed according to the recently adopted cyclic testing procedure ASTM E2126. A total of forty-five walls were tested with various configurations. The size of the walls was 1.2 x 2.4m (4 x 8ft). Two tests were performed with each sheathing material subjected to each type of loading: monotonic, cyclic with hold-downs, and cyclic without hold-downs. Two tests were then performed with OSB, hardboard, or fiberboard on one side of the wall and gypsum on the other side of the wall to study the effects of using dissimilar sheathing materials on the shear walls. The OSB and hardboard exhibited similar performance, and were the strongest of the four sheathing materials. Fiberboard performed better than gypsum, but worse than OSB and hardboard. In general, the performance indicators decreased when the walls were subjected to cyclic loading. The contribution of gypsum to walls with hold-downs was significant, but was not linearly additive. The use of hold-downs had a large effect on the performance of the walls. All shear wall performance indicators decreased when hold-downs were not included, with an average reduction of 66% in the peak load. / Master of Science

In this investigation, thirty-six shear walls measuring 8 feet (2.4 m) in width and possessing heights of 8, 9 and 10 feet (2.4, 2.7 and 3.0 m) were subjected to the reversed, cyclic loading schedule of the standard CUREE protocol in order to determine the performance capabilities of shear walls greater than 8 feet (2.4 m) in height sheathed with long panels. Of the thirty-six walls, a total of twelve walls measuring 9 and 10 feet (2.7 and 3.0 m) in height were sheathed with 4 x 8 feet (1.2 x 2.4 m) panels which required additional blocking members between the studs of the frame. Values obtained from the tests performed on these walls provided a direct comparison to those obtained from the walls of equal height, but sheathed with a long panel capable of spanning the entire height of the wall. The capabilities of long panels were investigated when used as the sheathing elements of shear walls with and without a mechanical hold-down device attached to the base of the end stud. An advantage of the long panel was investigated in which it was extended past the bottom plate and down onto the band joist to determine if significant resistance to the uplift present in walls without mechanical hold-down devices could be provided. Also, the effects of orienting the fibers of a 4 x 9 feet (1.2 x 2.7 m) panel in the alternate direction were examined. Average values of the parameters produced by walls sheathed with long panels either matched or exceeded those of its counterpart sheathed with 4 x 8 feet (1.2 x 2.4 m) panels in all configurations except the 10 feet (3.0 m) tall wall without hold-down devices. In fact, 4 x 9 feet (1.2 x 2.7 m) panels increased the performance of 9 feet (2.7 m) tall walls equipped with hold-down restraint significantly. Extending the long panels past the bottom plate and down onto the band joist improved the performance of both 8 and 9 feet (2.4 and 2.7 m) tall prescriptive shear walls significantly. Walls sheathed with panels made of fibers oriented in the alternate direction performed identically to those sheathed with panels of typical fiber orientation until the point of peak load. Once peak load was reached, walls sheathed with panels of alternate oriented fibers failed in a more sudden and brittle manner. / Master of Science

This thesis presents the results of testing to determine the significance of the group action factor at the 5% offset yield and capacity of single-shear bolted wood connections loaded parallel to grain. The single and multiple-bolt connections tested represent common connection geometries used in wood construction in the United States. The results of both monotonic and cyclic loading of connections are presented. Monotonic test data was used to determine an appropriately scaled CUREE Displacement Controlled Quasi-Static Cyclic Protocol. Overall, one hundred and eighty connections were tested using this cyclic protocol based on data obtained from thirty-three monotonic tests. Tested assemblies had geometric variables that include number of bolts per row, number of rows, bolt diameter, and side member material. In addition, the main and side member material and thickness were designed to produce three of the four major connection yield modes as defined by the 1997 National Design Specification for Wood Construction (AF&PA, 1997). Results from this research address the need for adequate spacing of bolts in a row to control the brittle connection behavior that directly affected the group action factor at capacity. / Master of Science

The majority of the physical properties of tissue depend directly on the interstitial or intracellular concentration of water within the epidermal and dermal layers. The relationship between skin constituent concentrations, such as water and protein, and the mechanical and optical properties of human skin is important to understand its complex nature. Localized mechanical loading has been proven to alter optical properties of tissue, but the mechanisms by which it is accomplished have not been studied in depth. In this thesis, skin's complex nature is investigated experimentally and computationally to give us better insight on how localized mechanical loading changes tissues water content and its optical properties. Load-based compression and subsequent increased optical power transmission through tissue is accomplished to explore a relationship between localized mechanical loading and tissue optical and mechanical properties. Using Optical Coherence Tomography (OCT), modification of optical properties, such as refractive index, are observed to deduce water concentration changes in tissue due to mechanical compression. A computational finite element model is developed to correlate applied mechanical force to tissue strain and water transport. Comprehensive understanding of the underlying physical principles governing the optical property changes within skin due to water concentration variation will enable future development of applications in the engineered tissue optics field. / Master of Science