Typescript (photocopy). / Digitized by Kansas Correctional Industries

Photocopy of typescript. / Digitized by Kansas Correctional Industries

At cable winding today one uses one portal, one caterpillar, one spooling boom and one tower and the same kind of set of equipment for unwinding. This kind of solution is both expensive and unpractical when one of the components of unwinding/winding is stationary for a long time without being used. To save unnecessary expenditure and maintenance it would have been practical to only use one set of caterpillar, tower, spooling boom and portal that later on can be rotated around the turntable when exporting the cable. The project that this work includes is to bring a concept that excludes two sets and thus provides the system with a rotatable spooling system. To get as much information about the product and of the manufacturing as possible several interviews has been done of the forging shop Ronneby Svets & Smide and also their fitters. At the concept generation several proposals was developed and to go further with only one of them a QFD and a SWOT analysis was made to get the best concept. Verification of solid mechanics on the construction with FEM-analysis has been implemented on beams, tubes and tube constructions. The project comprises constructing of details and drawings for structure of the concept and also commitment of assembly, maintenance, ergonomics and shipping. At the assembly has bolted joints been used on most attachments for fast assembling on site instead of welds. For commitment of maintenance it is thought that one should easily access for control and tighten afterwards of example the slewing ring. At the ergonomics the idea is put for example to accessing the workspace or maintenance areas in form of a ladder or a staircase. The shipping has played a major role for the construction parts and has then been divided for easiest and most practical way to be transported smoothly.

The aim of this study was to investigate the effect of frequent operational changes on the repairs and maintenance of public lighting in the Energy and Electricity Division of the City of Tshwane Metropolitan Municipality as a result of management decisions. The results of the study showed contrasting opinions and perceptions amongst all participants on the repairs and maintenance activities of public lighting. Both employees working in the maintenance operations and public lighting sections agreed that their sections were performing the repairs and maintenance of public lighting satisfactory. Customers and community leaders on the other hand agreed that the maintenance operations sections are performing the repairs and maintenance of public lighting satisfactory as compared to public lighting section. All employees were in favour and support of decisions taken respectively since these decisions were beneficial to their respective sections, but however they were concerned on the frequency of decisions taken by top management and changes implemented within their respective sections as these decisions and changes created confusion and conflict amongst employees. All sections involved in the repairs and maintenance of public lighting were faced with numerous similar challenges that created deficiencies and ineffectiveness on the maintenance activities of public lighting and that contributed to enormous complaints from customers and community leaders on the service rendered by the division.

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BACKGROUND:Obesity is an urgent public health problem, yet only a few clinical trials have systematically tested the efficacy of long-term weight-loss maintenance interventions. This randomized clinical trial tested the efficacy of a novel mind and body technique for weight-loss maintenance.METHODS:Participants were obese adults who had completed a six-month behavioral weight-loss program prior to randomization. Those who successfully lost weight were randomized into either an experimental weight-loss maintenance intervention, Tapas Acupressure Technique (TAT(R)), or a control intervention comprised of social-support group meetings (SS) led by professional facilitators. TAT combines self-applied light pressure to specific acupressure points accompanied by a prescribed sequence of mental steps. Participants in both maintenance conditions attended eight group sessions over six months of active weight loss maintenance intervention, followed by an additional 6 months of no intervention. The main outcome measure was change in weight from the beginning of the weight loss maintenance intervention to 12 months later. Secondary outcomes were change in depression, stress, insomnia, and quality of life. We used analysis of covariance as the primary analysis method. Missing values were replaced using multiple imputation.RESULTS:Among 285 randomized participants, 79% were female, mean age was 56 (standard deviation (sd) = 11), mean BMI at randomization was 34 (sd = 5), and mean initial weight loss was 9.8 kg (sd = 5). In the primary outcome model, there was no significant difference in weight regain between the two arms (1.72 kg (se 0.85) weight regain for TAT and 2.96 kg (se 0.96) weight regain for SS, p < 0.097) Tests of between- arm differences for secondary outcomes were also not significant. A secondary analysis showed a significant interaction between treatment and initial weight loss (p < .036), with exploratory post hoc tests showing that greater initial weight loss was associated with more weight regain for SS but less weight regain for TAT.CONCLUSIONS:The primary analysis showed no significant difference in weight regain between TAT and SS, while secondary and post hoc analyses indicate direction for future research.TRIAL REGISTRATION:ClinicalTrials.gov: NCT00526565

The adrenal cortex is classically divided into three morphologically and biochemically distinct zones, covered by a thin, cellular capsule. The adult adrenal cortex is a dynamic tissue in which distinct regions of cell proliferation, movement and death have been identified. Several models for stem cell maintenance of the adult adrenal cortex have been proposed, but adrenocortical stem cells have not yet been identified. Adrenal cortices of 21OH/LacZ transgenic mice show similar mosaic patterns of β-galactosidase staining to X- inactivation mosaics and LacZ ↔ wildtype chimeras. 21OH/LacZ mice provide a tool for lineage analysis, which may help to i) identify clones of cells produced by stem cells in the adult, ii) determine when stem cells begin to function and iii) evaluate different models of how stem cells maintain the adrenal cortex. Analysis of 21OH/LacZ transgenic adrenal cortices showed that the randomly orientated clusters of fetal patches change progressively during the perinatal period to adult radial stripes. Correlation of changes in mosaic patterns and the locations of cell proliferation suggests that the stripes arise by edge-biased growth during the perinatal growth period. Although stem cells may not be involved in the initial formation of stripes, it seems likely that stem cells later maintain the stripes by producing clones of cells that move centripetally to displace the earlier fetal patterns and later replace aging cells. Various combinations of BrdU labelling and chase periods demonstrated that most cell division occurred in the outer 40% of the adrenal cortex, confirmed that cells moved towards the medulla and identified a population of label-retaining cells near the capsule, which could include stem cells. (Stem cells have been recognised as BrdU label-retaining cells in other tissues because they divide less frequently than their daughter cells so dilute the incorporated BrdU more slowly.) Stripe patterns in adult 21OH/LacZ transgenic adrenal cortices were examined to try to distinguish between various models proposed for stem cell maintenance of the adrenal cortex. The observed continuous radial stripe pattern favours the general hypothesis that a single population of stem cells in the periphery maintains the entire adrenal cortex, although other explanations are possible. Quantitative analysis of adult stripe patterns did not show the reduction in stripe number that might be predicted if an age-related decline in adrenocortical stem cell function occurs, as may happen in some other tissues.

The adrenal gland is an important endocrine organ, protecting the body against acute and chronic stress. The adrenal cortex consists of three morphologically and functionally distinct zones: the outer zona glomerulosa (zG), the zona fasciculata (zF), and the innermost zona reticularis (zR). In rodents, zG cells produce mineralocorticoids (mainly aldosterone), while zF cells secrete glucocorticoids (mainly corticosterone). The functions of zG and zF are defined by the mutually exclusive expression of Cyp11b2 and Cyp11b1 that encode the enzymes aldosterone synthase and 11β-hydroxylase, which catalyze the terminal reactions in the production of aldosterone and corticosterone, respectively. This thesis aims to investigate the maintenance of the definitive mouse adrenal cortex. This involves studies to identify the location of adrenal stem/progenitor cells, and the mechanisms by which differentiated adrenocortical cells are replenished in the adult mice. BrdU pulse-chase studies provided valuable information about cell division and cell fate under physiological or pathophysiological stimulations. The distribution of adrenocortical cells with nuclei stained positively for BrdU and/or Ki67 was identified. Ki67 labelling marked actively dividing cells and showed that adrenocortical cells originate at or around the zG/zF interface. BrdU labelling indicated that, following cell division, cells are displaced inwards and outwards. Acute angiotensin II treatment was shown to have no significant effects on the cell proliferation or turnover in any of the adrenocortical zones. The pathophysiological effects of long-term ACTH treatment were analyzed in a mouse model of congenital adrenal hyperplasia caused by a null mutation of Cyp11b1. Cell hypertrophy was evident in all regions of the adrenal cortex due to the impaired negative-feedback of the HPA axis. Adrenocortical cell proliferation was also increased particularly in the outer zona fasciculata at the border between zG and zF where adrenocortical stem/progenitor cells might be located. The intervening steps between cell proliferation and the final differentiation into steroidogenic zG and zF cells have yet to be discovered. A visual method of monitoring levels of Cyp11b2 and Cyp11b1would offer a convenient approach to track the stages of adult stem cell differentiation that lead to normal adrenal maintenance in vivo and in vitro. In the present study an AS-mCherry-11B-EGFP BAC construct was successfully engineered, in which Cyp11b2 and Cyp11b1 were substituted by mCherry and EGFP, respectively. This BAC construct was characterized in mouse adrenocortical Y1 cells. It was determined that EGFP faithfully recapitulated the expression of Cyp11b1. Forskolin or cAMP treatment induced a rapid cell rounding effect and caused the increased expression of EGFP transgene and endogenous Cyp11b1. An attempt was made to establish a transgenic mouse model, in which zG and zF cells would be marked with mCherry and EGFP respectively, allowing the differentiation of an adrenocortical stem cell to be traced. Following microinjection of the BAC into mouse zygotes, twoAS-mCherry-11B-EGFP transgenic founder mice were identified. Unfortunately, neither of them was able to transmit the transgene through germline, suggesting the mosaicism of transgene integration. Indeed, mosaicism of the transgenic adrenals was demonstrated by RT-PCR and immunostaining, which also revealed that the exogenous EGFP expression faithfully recapitulated the endogenous Cyp11b1 in adrenals. Although it is assumed that expression of Cyp11b2 and Cyp11b1 are mutually exclusive, zG and zF cells may have the plasticity to allow the transition from one cell type into another. The AS-mCherry-11B-EGFP BAC construct is a useful tool for studying in vitro ES cell differentiation towards the adrenocortical lineage. Transgenic AS-mCherry-11B-EGFP ES cells were successfully differentiated into mesenchymal stem cells, as identified by the expression of molecular markers for the mesenchymal lineage. It has been reported that steroidogenic factor (Sf1) can promote the differentiation of MSCs into steroidogenic cells, and Shh plays an important role in Sf1 expression and the consequent adrenal development. However, Shh treatment failed to achieve transformation of mesenchymal cells into adrenocortical cells. It is thought there might be a requirement for additional factors to combine with Shh in promoting the transdifferentiation of ESC-derived mesenchymal cells. Future studies will focus on the genetic control of Cyp11b1 and Cyp11b2 in transgenic AS-mCherry-11B-EGFP ES cells. In conclusion, the location and fate of the adrenocortical progenitor cells were demonstrated by the BrdU pulse-chase studies in different mouse models. An AS-mCherry-11B-EGFP BAC construct was generated, and used to study the mutual and differential controls of Cyp11b1 and Cyp11b2 expression in adrenocortical cells in vitro and in transgenic mice in vivo.

Unlike Sweden, Denmark is a major industry when it comes to christmas tree cultivation. More than ten million christmas trees are processed in Denmark annually. The primary purpose of this report is to investigate the technology, treatment and maintenance of christmas trees in Sweden and Denmark, and to find differences in the management between Swedish and Danish christmas tree cultivators. Since an opinion poll has been made, the investigation method mainly used for this report is quantitative. But a qualitative investigation method has also been used to some extent as a few unstructured interviews has occurred while visiting a couple of the cultivators.   63 % of the christmas tree cultivators consider themselves knowledgeable about christmas tree cultivation and 68 % wants to learn more. There is both similarities and differences along those cultivators that has been visited. Obvious similarities has mostly been found when visiting farms engaged in developing christmas trees to a bigger extent. Denmark conducts their maintenance methods with the aim to produce a large amount of high quality Christmas trees. This tendency does also occur in Sweden at, for example, Gunnars Granar located in Degeberga. Gunnar manages his cultivation almost identically to Denmark's management methods. However, the big christmas tree cultivators are still mainly located in Denmark.

published_or_final_version / Industrial Engineering / Master / Master of Science in Engineering