Thesis (M.S.)--University of Michigan, 1961.

Intravenous application of high dose of capsaicin to the rat stimulates C-fiber neurons that innervate the airways to release tachykinins that produce acute inflammation in the mucosal tissue. Large amount of extravasated plasma is retained underneath the tracheal epithelium to form edema. Substance P (SP) is the most important inflammation-producing peptide of tachykinin family. The present study was to investigate time-dependent formation and remission of edema induced by SP (3 µg/ml/kg) by the use of digital morphometric analysis of montages of tracheal cross sections. Furthermore, 3-dimensional reconstruction of serial tracheal sections was carried out to analyze the relative distribution of subepithelial edematous loci. Two edema indexes were designated for evaluation of the status of edema. Edema length ratio was the ratio of the total length of edematous loci to the circumference of a tracheal section. Edema area ratio was the ratio of the total area of edematous loci to the area of tracheal epithelium and associated edema. The degree of edematous status in the mucosa exhibited a time-dependent change. Five min after application of SP, edema length ratio and edema area ratio in the trachea were 35.80¡Ó1.42% and 16.28¡Ó2.51%, that were 7.6 and 7.9 times, respectively, the values of vehicle control group. At 1 h after SP, edema length ratio and edema area ratio declined to 16.40¡Ó2.46% and 8.00¡Ó1.60%, 2.2 and 2.8 times the values of control, but still significantly different (P < 0.05). At 24 or 72 h after SP, the values of edema were not significantly different (P > 0.05) from the control values. Three- dimensional reconstruction study showed that, in the trachea of rats 5 min after receiving SP, there were many subepithelial edematous loci, evenly distributed along the inner circumference of trachea. They were interconnected. The number of edematous loci decreased drastically by 1 h after SP. Loci of edema were rarely found 24 or 72 h after SP. The close association of edema to the tracheal epithelium suggests that the mucosal surface may be the site for elimination of edema fluid.

Thesis--Ph. D., University of Hong Kong. / Typewritten.

published_or_final_version / Nursing Studies / Master / Master of Nursing

published_or_final_version / Physiology / Doctoral / Doctor of Philosophy

Thesis (M.S.)--Indiana University, 2006. / Includes bibliographical references. Also available online (PDF file) by a subscription to the set or by purchasing the individual file.

Thesis (Honors)--Ohio State University, 2004. / Title from first page of PDF file. Document formatted into pages; contains 31 p.; also includes graphics. Includes bibliographical references (p. 22-23). Available online via Ohio State University's Knowledge Bank.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1983. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1984. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographies.

WANDERLEY, Carlos Wagner de Souza. Veneno da serpente Bothrops jararacussu induz uma resposta inflamatória local dependente de protanóides e da migração de neutrófilos. 2014. 93 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2014. / Submitted by denise santos (denise.santos@ufc.br) on 2014-08-27T13:12:18Z No. of bitstreams: 1 2014_dis_cwswanderley.pdf: 1416460 bytes, checksum: 8eb00a82635e311b2d474e81e1c9e47e (MD5) / Approved for entry into archive by denise santos(denise.santos@ufc.br) on 2014-08-27T13:30:45Z (GMT) No. of bitstreams: 1 2014_dis_cwswanderley.pdf: 1416460 bytes, checksum: 8eb00a82635e311b2d474e81e1c9e47e (MD5) / Made available in DSpace on 2014-08-27T13:30:45Z (GMT). No. of bitstreams: 1 2014_dis_cwswanderley.pdf: 1416460 bytes, checksum: 8eb00a82635e311b2d474e81e1c9e47e (MD5) Previous issue date: 2014 / Local tissue reactions provoked by Bothrops venoms are characterized by edema, hemorrhage, pain, and inflammation; however, the mechanisms of tissue damage vary depending upon the species of snake. This mechanistic variability reduces the efficacy of antivenom treatment. Considering the diversity of intraspecific pathophysiological responses, we investigated the mechanisms and mediators involved in the local inflammatory response induced by the Bothrops jararacussu venom (VBjsu). Female Swiss mice were injected with either saline or VBjsu (0.125-8 µg/paw). In a subset of VBjsu-treated mice, loratadine (an H1 receptor antagonist), compound 48/80 (for mast cell depletion), capsaicin (for C-fiber desensitization), infliximab (an anti-TNF-α antibody), indomethacin (a non-specific COX inhibitor), celecoxib (a selective COX-2 inhibitor) or fucoidan (a P- and L-selectins modulator) were administered before VBjsu treatment. Paw edema was measured by plethysmography. In addition, paw tissues were collected for the measurement of myeloperoxidase (MPO) activity, TNF-α and IL-1 levels, and COX-2 immunoexpression. The direct chemotactic effect of VBjsu was also studied using a Boyden chamber assay, and the in vitro calcium dynamic in neutrophils was investigated using confocal microscopy. VBjsu caused concentration- and time-dependent edematogenic responses and increased the local production of TNF-α and IL-1β as well as COX-2 expression vs. saline group (P<0.05). Both edema and neutrophil migration were prevented by pretreatment with cyclooxygenase inhibitors (indomethacin or celecoxib) or by the P- and L-selectins modulator, fucoidan vs. VBjsu (P<0.05). Furthermore, VBjsu induced a direct in vitro neutrophil chemotaxis by increasing intracellular calcium vs. RPMI (P<0.05). Therefore, VBjsu induces an early onset edema dependent upon prostanoid production and neutrophil migration. / Os efeitos locais provocados por venenos botrópicos são caracterizados por edema, hemorragia e dor local. No entanto, os mecanismos fisiopatológicos relacionados a esses venenos podem variar de acordo com a espécie. Esta característica espécie-específica pode prejudicar a eficácia da soroterapia. Desta forma, considerando a diversidade da resposta fisiopatológica dos venenos, investigamos os mecanismos e mediadores envolvidos na resposta inflamatória local induzida pelo veneno da Bothrops jararacussu (VBjsu). Camundongos Swiss fêmeas foram submetidos à injeção intraplantar de solução salina ou VBjsu (0,125-8 µg/pata). Para o estudo dos mecanismos e mediadores envolvidos, diferentes sub-grupos de animais foram submetidas ao pré-tratamento com loratadina (um antagonista do receptor H1), composto 48/80 (para induzir a depleção dos mastócitos), capsaicina (para promover a dessensibilização das fibras C), infliximabe (um anticorpo anti-TNF-α), indometacina (um inibidor não-específico da COX), celecoxibe (um inibidor seletivo da COX-2) ou fucoidina (um modulador de P- e L-selectinas) administrados antes do VBjsu. O edema da pata foi medido por pletismografia. Além disso, os tecidos da pata foram recolhidos para determinação da atividade da mieloperoxidase (MPO), dosagem dos níveis de TNF-α, IL-1 e da imunoexpressão da COX-2. O efeito quimiotático direto do VBjsu também foi estudado por meio do ensaio da câmara de Boyden, e o estado de ativação dos neutrófilos humanos foi avaliado através da dinâmica intracelular de cálcio em neutrófilos realizado in vitro com auxilio da microscopia confocal. O VBjsu causou uma resposta edematogênica concentração e tempo dependentes com aumento da produção local de TNF-α, IL-1β e da imunoexpressão da COX-2 nos tecidos quando comparado ao grupo salina (P<0,05). Ambos, edema e migração de neutrófilos foram impedidos pelos inibidores da COX (indometacina ou celecoxibe), ou pelo modulador de P - e L-selectinas (fucoidina) vs. VBjsu (P<0,05). O exame histopatológico revelou que o VBjsu induz uma precoce migração de neutrófilos para o local da lesão vs. o grupo salina (P<0,05). Além disso, o VBjsu induz a quimiotaxia e ativa neutrófilos in vitro aumentando diretamente a dinâmica de cálcio intracelular quando comparado ao RPMI (P<0,05). Portanto, o VBjsu induz uma resposta edematogênica precoce dependente da produção de prostanoides e da migração de neutrófilos.