RNA editing in trypanosomes is the post-transcriptional insertion or deletion of uridylates at specific sites in mitochondrial mRNAs. This process is catalyzed by a multienzyme, multisubunit complex through a series of enzymatic cycles directed by small, trans-acting RNA molecules. Despite impressive progress in our understanding of the mechanism of RNA editing and the composition of the editing complex, fundamental questions regarding RNP assembly and the regulation of catalysis remain. This dissertation presents studies of RNA-protein interactions between RNA editing complexes and substrate RNAs and the determination of substrate secondary structural determinants that govern them. Our results suggest that substrate association, cleavage and full-round editing by RNA editing complexes in vitro obey hierarchical determinants that increase in complexity as editing progresses and we propose a model for substrate recognition by RNA editing complexes. In addition, this dissertation also presents the characterization of a novel mitochondrial RNA helicase, named REH2 and its macromolecular interactions. Our data suggest that REH2 is intimately involved in interactions with macromolecular complexes that integrate diverse processes mediating mitochondrial gene expression. These results have implications for the mechanism of substrate RNA recognition by RNA editing complexes as well as for the integration of RNA editing to other facets of mitochondrial RNA metabolism.

Thesis (Ph. D.)--University of Washington, 2002. / Vita. Includes bibliographical references (p. 101-108).

Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 164-175).

My thesis film for the Master of Fine Arts is a twelve-minute experimental film entitled You and me are stitched. It follows three friends, Rosine, Sandy and Travis. It is a film that thinks around their curious triangle. This report is an account of the evolution of its filmmaking concept and process, from the initial idea through the finished film. The finished film bears little similarity to the original intent, so I pay particular attention to discussing conceptual breakthrough and post-production discoveries in this report. / text

Studying the diverse, experimental, and unconventional work—particularly poetry—of Canadian writer bpNichol requires a better understanding of the material characteristics he used to give his writing unique significance. Nichol’s poems call for a plurality of new analytical methods, since traditional editorial and critical approaches often overlook the importance of materiality. This thesis presents three different approaches to critically considering Nichol’s work and highlighting its material aspects: comparing Nichol’s poetry to Dada aesthetics, examining multiple versions of individual poems using genetic criticism, and looking at the changes and similarities between print and digital material characteristics from the even wider perspective of media archaeology. Additionally, the benefits of a digital edition are argued in relation to all three editorial approaches, as digital presentation augments the focus on the materiality that is so integral to reading Nichol.

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The promotion of Shakespeare to the centre of the English literary canon was largely facilitated by ten major eighteenth-century editions of his plays: by Nicholas Rowe (1709), Alexander Pope (1723-25), Lewis Theobald (1733), Thomas Hanmer (1744), William Warburton (1747), Samuel Johnson (1765), George Steevens (1766), Edward Capell (1767-68), Johnson and Steevens (1773) and Edmond Malone (1790). The popularity of Newtonian science in eighteenth-century England helps to explain the mentality that impelled this energetic enterprise. In their Prefaces, the editors describe Shakespeare as a Newton-like genius who understood the underlying principles of human nature and expressed them through his characters. Shakespeare, however, unlike Newton, was not a systematic thinker, and the editors are critical of his language and of his tendency to cater to the low tastes of the Elizabethan theatre. They view him as a genius who understood fundamental truths about human nature and, at the same time, metaphorically, as nature itself---a site of heterogeneity and confusion where the editor must find hidden knowledge. They figure themselves as, scientists charged with the task of altering, restoring and annotating Shakespeare's writings. In the editions leading to and including that of Johnson, the editors' focus is on the universality of Shakespeare's discoveries. The early editors promote a transcendental image of Shakespeare as a timeless genius who rose above the relatively barbaric age in which he lived. The two editors following Johnson, however, place an increasing emphasis on Shakespeare's Englishness. While the idea of Shakespeare as a universal genius persists, Steevens and Capell also view him as a specifically English figure whose writings are to a large extent a product of his society. This nationalist emphasis goes hand in hand with an increasingly historical approach to the annotation and textual restoration of Shakespeare. The development of editing as a professional scientific vocation culminates with Malone, who augmented the editorial apparatus with thoroughly researched accounts of Shakespeare's life and theatre. The persistent emphasis on knowledge in the editors' work helps to account for the rise of Shakespeare's canonicity in relation to the Newtonian truth-seeking project of the eighteenth century.

Twenty-two United Methodist Church (UMC) editors Q-sorted fifty-four news stories in this research study. The concourse was constructed using six news values mainly used by editors and reporters in the commercial news media: conflict, impact, magnitude, prominence, novelty, and proximity. The stories were sorted along an eleven point bi-polar continuum from "most important" to "least important"The study revealed that two kinds of editor perceptions emerged in the UMC: the denominational editors who selected news stories based on the proximity news element, and the ecumenical editor, who selected news stories based on the news elements of magnitude, impact, and novelty.Despite the use of these news values, the study also showed that the environmental factors such as organizational policies of the UMC and the bishops, influenced story selection for publication. / Department of Journalism

This creative project consists of a short fiction film which is finished on DVD and features a full length video commentary. The video commentary is an invention of my own which utilizes an often overlooked element of DVD technology, the multi-angle ability, to effectively merge two of the most popular features of modern motion picture DVD releases: the director's commentary and the production documentary. The disk actually contains two tracks of video, each of identical length. One is the film; the other is a documentary on the making of the film, with certain sections that correspond in real time with events in the film. The user has the ability to switch between tracks at anytime for comparison. This to my knowledge has never been done before, and because of the enormous popularity of the DVD and these features, may find widespread application. This project, while only an exploration of its potential, shows that the idea has feasibility and legitimacy. / Department of Telecommunications

Despite the importance of the subject to the discipline of Middle English studies, little research has been published on the history of the editing of Middle English texts. This thesis supplies a small, but essential portion of that history by examining the editorial practices that were used to produce the editions of Middle English texts published by the Early English Text Society in the nineteenth century. Then the dominant publisher in its field, EETS identified and printed almost the entire Middle English canon during a crucial time in the development of English studies the period in which it moved from being an almost exclusively amateur pursuit to one accepted and practiced by professional academics in the universities. To provide a context for my examination of EETS editions, I first investigate the financial and material conditions under which EETS' publications were produced and examine the ideas which guided EETS' editorial policy in the light of contemporary theories about the editing of Middle English texts. I then examine nine editions in detail, analysing the various methods by which the text is established and formal manuscript detail is represented in print. The analysis contained in these nine studies is based on the evidence I compiled by comparing sample extracts of the printed text and associated paratext of each edition with the manuscript evidence originally available to the editor. I then use the information gathered about these individual editions as part of an assessment of the editorial practices that define the nature of EETS' nineteenth-century editorial output as a whole. I find that a conservative editorial approach that valorises the evidence of individual manuscripts characterises the majority of EETS' publications, but that the Society also produced a great variety of editions that diverge from this approach, including several of the earliest applications of recension to Middle English texts published in England.