The objectives of this thesis were to investigate some physiological, cellular and molecular changes from genetic selection, flock age and egg storage on embryonic metabolism, cell viability and gene expression in poultry species. Embryonic heat production (EHP), the parameter used to evaluate metabolic status of two turkey genetic strains (Nicholas and Hybrid) and four flock ages (30 wk, 34 wk, 55 wk, 60 wk) were determined. The results showed that embryos of older flocks had higher EHP than all other flock ages. The higher metabolism can cause overheating in embryos from older flocks during the later part of incubation. The EHP values can assist incubator manufacturers and hatchery managers to manage incubation temperatures to increase embryo survival. The EHP was also determined in two Ross 308 broiler breeder egg storage durations (4 d and 14 d) to assess the impact of long-term egg storage on embryonic metabolism. The embryos from 4 d stored eggs had higher body weight and EHP than embryos from 14 d stored eggs. To investigate what could be the fundamental reasons for the metabolic differences, blastoderms from eggs in these egg storage treatments were isolated, dissociated into single cells, stained with fluorescent dyes and subjected to flow cytometry analysis to differentiate between viable (live), apoptotic and necrotic cell populations. Quantitative real time PCR analysis was then used to compare the expression of selected apoptotic genes (Bak, Bax, Bok, Bcl-2, Bcl-xL) in blastoderms and embryos from 6 d incubated eggs. While percentage of viable cells decreased significantly, an unregulated amount of apoptosis increased with egg storage duration (p < 0.0001). Expression of pro-apoptotic genes (Bak, Bax, and Bok) were up regulated as storage duration increased at the blastodermal level but all genes were down regulated after 6 d of incubation. This suggests that an increment in egg storage duration can cause activation of apoptotic cell death mechanisms at the blastodermal level, which can be one of the molecular mechanisms that led to the reduced daily embryonic growth and metabolism observed in 14 d stored eggs. The thesis suggests there are molecular and cellular implications of egg storage. / Animal Science

Many bioactive peptides have been reported from various food proteins through the conventional activity-guided-purification approach; however, the rationale behind the selection of conditions for the production of the bioactive peptides has not been extensively explored. The purposes of the study were to provide the rationale behind the selection of conditions, and to develop an innovative strategy to explore the most potent peptides within egg proteins through an integrated QSAR and bioinformatics approach. Thermolysin-pepsin hydrolysate of ovotransferrin was predicted as the best condition for production of ACE-inhibitory peptides. Three predicted peptides, IRW, LKP and IQW, were successfully released from ovotransferrin. Simulated gastrointestinal incubation showed IQW was stable while IRW and LKP can be degraded into dipeptides (IR and KP respectively). Peptides produced from the study will have the potential to be developed as functional foods and nutraceuticals for the prevention of hypertension, a disease affecting ~ 31% of the adult population. / Food Science and Technology

Previous work has suggested that triclabendazole, a member of the benzimidazole group of compounds, possessed efficacy against Schistosoma mansoni. In view of recent indications in praziquantel treatment failures and loss of sensitivity, it is imperative that new anti-schistosomals are developed as contingent treatment options, while resistance alleles, if any, remain at low frequencies. While recent studies have indicated that triclabendazole monotherapy exert weak anti-schistosomal effects, the combinatorial application of triclabendazole with praziquantel has not been explored. To assess this hypothesis, triclabendazole and its metabolites were initially assessed against the many life-stages of Schistosoma mansoni in vitro. Combinatorial drug and isobologram analyses against adult Schistosoma mansoni was also performed, and subsequently applied against other parasitic models (Giardia duodenalis and Haemonchus contortus) to assess the specificity of such effects. Subsequently, the drug combinations were assessed against Schistosoma mansoni in vivo. To further assess the suitability of combinatorial drug applications, an economic model was developed to project the cost-efficacy of praziquantel-triclabendazole drug combinations in a global focus. It was concluded that triclabendazole and its metabolites possessed good efficacy against immature schistosomula, albeit weak efficacy against adult Schistosoma mansoni. Upon combination with praziquantel, however, a strong synergistic effect against adult worms were observed in vitro. Praziquantel and triclabendazole were also shown to possess unique and independent ovicidal modes of action that can be of clinical significance. More importantly, in vivo drug trials concluded that the combinations exerted additive effects against Schistosoma mansoni harbored in mice. Economic modeling and cost-effectiveness analyses further demonstrated the feasibility of this drug combination, and may represent a new line of treatment against mansonial schistosomiasis

Dysarthria is one of the most common signs of speech impairment in the cerebral palsy (CP) population. Facilitating strategies for speech enhancement in this population often include training on speech breathing. Treatment efficacy studies with cross-system measures in this population are needed for improved understanding and management of the interrelationship between respiratory, phonatory, and articulatory systems. The purpose of this study was to investigate the effect of breath group control on the coordination of articulatory and phonatory muscles and the acoustic measures related to speech and voice quality. A simultaneous acoustic, electroglottographic (EGG), and marker-based facial tracking recording system was employed to monitor the speech production behaviors of four adults with CP and 16 neurologically healthy controls. Subjects were instructed to perform three tasks, each containing speech targets with a voiceless plosive (/p/, /t/, or /k/) preceding a vowel (/i/, /a/, /u/, or /ɔ/). Task 1 consisted of a short reading passage embedded with target vowels without cueing from breath group markers. Task 2 included reading a series of monosyllabic and 3-syllable or 5-syllable non-speech words with the speech targets. Task 3 included reading the same short passage from Task 1 with cueing from breath group markers separating the passage into phrases with no more than five syllables per phrase. Measures from the acoustic, EGG and facial tracking recordings of the first and last syllable of all syllable trains produced in the non-speech task and the target vowels in the passage reading task were examined. Acoustic measures included voice onset time (VOT), vowel duration, fundamental frequency (F0), percent jitter (%jitter), percent shimmer (%shimmer), signal-to-noise ratio (SNR), and frequencies of Formants one and two (F1 and F2). EGG measures included speed quotient (SQ) and open quotient (OQ). Facial tracking measures consisted of maximum jaw displacement. Individual and averaged data were submitted to a series of two-way Analysis of Variances (ANOVAs) or two-way Repeated Measures ANOVAs to determine the effects of the relative position of an utterance in the breath group and the place of articulation of the consonants involved. In addition, mean vowel spaces derived from all three tasks were examined. Results revealed significant changes of VOT, F1, F2, SNR and SQ as a function of position. Significant changes of VOT, vowel duration, F2, F0, %jitter, %shimmer, and maximum jaw displacement as a function of place of articulation were also evident. In particular, breath group control was found to result in expansion of vowel space, especially for individuals with CP. These findings suggest that proper phrasing enhances articulatory and phonatory stability, providing empirical evidences in support of its usage in treating individuals with CP.

Most theoretical models predict an optimal offspring size that maximises parental fitness. Variation in the quality of the offspring environment can result in multiple offspring size optima and therefore variation of offspring provisioning can evolve. Variation in offspring provisioning is common and found across a variety of taxa. It can be defined as between populations, explained by optimality models, or between and within individuals, neither so easily explained by optimality models. My research focused on the evolution of variable offspring provisioning by testing theoretical models relating to variation in offspring provisioning between and within individuals. Using comparative methods, I found a positive relationship between intraclutch variation in offspring provisioning and variation in the quality of the offspring environment in a suite of pond breeding frogs. This positive relationship provided evidence that patterns of variable offspring provisioning are related to the offspring environment. This study also identified a species (Crinia georgiana) with high variation in offspring provisioning on which to focus further investigations. High variation in offspring provisioning occured between and within individuals of this species independent of female phenotype and a trade-off in offspring size and number existed. In laboratory studies, increased yolk per offspring led to increased fitness per offspring. Parental fitness calculations revealed that in high quality conditions production of small more numerous offspring resulted in higher parental fitness, but in lower quality conditions the production of large offspring resulted in the highest parental fitness. This was confirmed in field experiments under natural conditions using molecular markers to trace offspring to clutches of known provisioning, allowing me to measure exact parental fitness. The strategy of high variation in offspring size within clutches can be of benefit when the future of the offspring environment is not known to the parents: as a form of bet-hedging. Further study of the offspring environment revealed that conditions such as density dependent fitness loss, spatial variation in habitat quality, and non-random offspring dispersal, can combine to create the conditions predicted by theoretical models to maintain a strategy of variable offspring provisioning in the population. My research provides a comprehensive empirical test of the theory of variable offspring provisioning

Diss. (sammanfattning) Uppsala : Univ., 2002. / Härtill 6 uppsatser.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2003.

Thesis (Ph. D. in Biological Sciences)--Vanderbilt University, May 2009. / Title from title screen. Includes bibliographical references.

Dissertation (Ph.D.)--Auburn University, / Abstract. Vita. Includes bibliographic references (p.116-129).

Thesis (M. Dev. Stud. (Development Studies))--University of the Free State, 2004. / Includes bibliographical references (p. 68).