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Stabilisation and activation of horseradish peroxidaseEddeb, Fadel January 1995 (has links)
No description available.
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Enzyme-immunoassay of alpha-foetoproteinHalliday, M. I. January 1980 (has links)
No description available.
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The preparation and application of monoclonal antibodies specific for phosphorylated isoforms of myelin basic proteinYon, Suzanne Michele January 1995 (has links)
No description available.
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Quantitation of Red-Cell-Bound IgG in Normal and Pathologic States by an Enzyme Immunoassay (EIA) TechniqueKATO, KANEFUSA, YAMADA, HIDEO, HIRANO, AKIHITO 01 1900 (has links)
No description available.
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In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quailShimada, Kiyoshi, Ha, Yonju, Tsukada, Akira, Saito, Noboru, Maekawa, Shinobu, Nishizuka, Makoto, Imagawa, Masayoshi January 2005 (has links)
No description available.
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Perfil analítico das progestinas fecais nas fases de puberdade e ciclicidade ovariana em Onça Pintada (Panthera onca); gestação e lactação em Gato Mourisco (Puma yagouaroundi) / Analytical profile of progestins during puberty and ovarian cyclicity in jaguar (Panthera onca); gestation and lactation in jaguarondi (Puma yagouaroundi)Guisso, Debora Cattaruzzi Rodini 20 August 2008 (has links)
O presente estudo teve como objetivo utilizar a técnica de enzimaimunoensaio (EIE) com o Ac monoclonal CL425 na dosagem de metabólitos de progestinas fecais para caracterizar o perfil no início da atividade ovariana e durante o ciclo estral em onça pintada (Panthera onca) e durante a gestação e lactação em gato mourisco (Puma yagouaroundi). Foram estudadas três fêmeas de onça pintada em fases distintas (pré-puberes n=2 e adulta n=1) e três fêmeas de gato mourisco em duas fases distintas (gestantes n=2 e lactantes n=3). O protocolo empregado no EIE foi validado para a mensuração de progestinas em fezes de onça pintada e gato mourisco (r=0,98, p=0,0078; r=0,97, p=0,0130, respectivamente). Observou-se que os animais pré-puberes apresentaram início da produção de progesterona nos meses de setembro e novembro. As elevações das progestinas fecais na fêmea adulta de onça pintada não se sustentaram, indicando que não ocorreu ovulação espontânea nessa espécie. Houve diferença significativa entre as concentrações médias de progestinas fecais (p<0,001) dos animais pré-púberes e adultos no grupo das fêmeas de onça pintada. No grupo das fêmeas de gato mourisco, não foi possível diferenciar as concentrações de progestinas fecais durante a gestação e lactação.Obtivemos correlação entre as concentrações de progestinas fecais medida pelos métodos de radioimunoensaio (RIE) e enzimaimunoensaio (r=0,98, p<0,0001). / The present study had as objective to use the technique of enzyme immunoassay (EIA) with monoclonal antibody CL425 in the dosage of faecal progestin to characterize the profile during ovarian activity beginning and estral cycle in jaguar (Panthera onca) and gestation and lactation in jaguarondi (Puma yagouaroundi). Three female jaguars were studied in distinct phases (pre-pubertal n=2 and adult n=1) and three female jaguarondi were studied during two distinct phases (gestation n=2 and lactation n=3). The EIA used was validated for faecal progestin measurement in jaguar and jaguarondi (r=0,98, p=0,0078; r=0,97, p=0,0130, respectively). The beginning of progesterone production for pre-pubertal animals was in September and November. Elevations of progestin in the adult jaguar were not supported, showing that there was not spontaneous ovulation for this specie. There was significant difference between medium progestin concentrations (p<0,001) of pre-pubertal and adult jaguars. It was not possible to identify different progestin concentrations during gestation and lactation in female jaguarondi. The faecal progestin profiles measured by radioimmunoassay (RIA) and enzyme immunoassay (EIA) corresponded well and were positively correlated (r=0,98, p<0,0001).
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Perfil analítico das progestinas fecais nas fases de puberdade e ciclicidade ovariana em Onça Pintada (Panthera onca); gestação e lactação em Gato Mourisco (Puma yagouaroundi) / Analytical profile of progestins during puberty and ovarian cyclicity in jaguar (Panthera onca); gestation and lactation in jaguarondi (Puma yagouaroundi)Debora Cattaruzzi Rodini Guisso 20 August 2008 (has links)
O presente estudo teve como objetivo utilizar a técnica de enzimaimunoensaio (EIE) com o Ac monoclonal CL425 na dosagem de metabólitos de progestinas fecais para caracterizar o perfil no início da atividade ovariana e durante o ciclo estral em onça pintada (Panthera onca) e durante a gestação e lactação em gato mourisco (Puma yagouaroundi). Foram estudadas três fêmeas de onça pintada em fases distintas (pré-puberes n=2 e adulta n=1) e três fêmeas de gato mourisco em duas fases distintas (gestantes n=2 e lactantes n=3). O protocolo empregado no EIE foi validado para a mensuração de progestinas em fezes de onça pintada e gato mourisco (r=0,98, p=0,0078; r=0,97, p=0,0130, respectivamente). Observou-se que os animais pré-puberes apresentaram início da produção de progesterona nos meses de setembro e novembro. As elevações das progestinas fecais na fêmea adulta de onça pintada não se sustentaram, indicando que não ocorreu ovulação espontânea nessa espécie. Houve diferença significativa entre as concentrações médias de progestinas fecais (p<0,001) dos animais pré-púberes e adultos no grupo das fêmeas de onça pintada. No grupo das fêmeas de gato mourisco, não foi possível diferenciar as concentrações de progestinas fecais durante a gestação e lactação.Obtivemos correlação entre as concentrações de progestinas fecais medida pelos métodos de radioimunoensaio (RIE) e enzimaimunoensaio (r=0,98, p<0,0001). / The present study had as objective to use the technique of enzyme immunoassay (EIA) with monoclonal antibody CL425 in the dosage of faecal progestin to characterize the profile during ovarian activity beginning and estral cycle in jaguar (Panthera onca) and gestation and lactation in jaguarondi (Puma yagouaroundi). Three female jaguars were studied in distinct phases (pre-pubertal n=2 and adult n=1) and three female jaguarondi were studied during two distinct phases (gestation n=2 and lactation n=3). The EIA used was validated for faecal progestin measurement in jaguar and jaguarondi (r=0,98, p=0,0078; r=0,97, p=0,0130, respectively). The beginning of progesterone production for pre-pubertal animals was in September and November. Elevations of progestin in the adult jaguar were not supported, showing that there was not spontaneous ovulation for this specie. There was significant difference between medium progestin concentrations (p<0,001) of pre-pubertal and adult jaguars. It was not possible to identify different progestin concentrations during gestation and lactation in female jaguarondi. The faecal progestin profiles measured by radioimmunoassay (RIA) and enzyme immunoassay (EIA) corresponded well and were positively correlated (r=0,98, p<0,0001).
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Evaluation and Determination of the Sensitivity and Specificity of a Treponema Pallidum Dried Blood Spot Method for Serologic Diagnosis of SyphilisTurgeon, David K. 20 December 2012 (has links)
EVALUATION AND DETERMINATION OF THE SENSITIVITY AND
SPECIFICITY OF A Treponema pallidum DRIED BLOOD SPOT (DBS) METHOD FOR
SEROLOGIC DIAGNOSIS OF SYPHILIS
Background:
Syphilis is a sexually transmitted infection (STI) caused by Treponema pallidum subspecies pallidum. Syphilis is known as the "great imitator" due to the similarity of clinical signs and symptoms to other infectious diseases. The primary diagnosis of syphilis relies on clinical findings, including the examination of treponemal lesions, and/or serologic tests. Serologic tests are divided into nontreponemal and treponemal tests. Nontreponemal tests are useful for screening, while treponemal tests are used as confirmatory tests.
Methods:
A total of 200 serum and DBS specimens collected from patients at the Los Angeles Municipal Sexually Transmitted Disease Clinics were tested by the DBS and enzyme immunoassay (EIA) methods. These samples were sent to the Syphilis Diagnostics Laboratory, Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia for testing. Samples were blindly evaluated by the TREP-SPOTTM DBS and the TREP- SURETM EIA methods for the detection of anti-treponemal IgG- and IgM-class antibodies.
Results:
The sensitivity of the DBS method was 83% (95% CI, 73.89 - 89.50) and specificity was 100% (95% CI, 95.39 - 100)). The positive predictive value and negative predictive values were 100% (95% CI, 94.48 - 100) and 85% (95% CI, 77.43 - 91.0), respectively. The efficiency of the DBS method was 91.5%. The kappa value for the agreement between the DBS method and EIA assay was 0.83 (95% CI, 0.754 - 0.906). The correlation coefficient (r2) between the anti-treponemal antibody assay results obtained from DBS and serum samples was 0.94.
Conclusion:
DBS is an optimal choice to be used as a screening tool for the detection of anti-treponemal antibodies for the diagnosis of syphilis. The detection of anti-treponemal antibodies (TREP-SPOTTM DBS EIA) compared favorably to the results of serum-base assay (TREP-SURETM EIA), with an overall concordance of 91.5%. Dried blood spots are technically easier to obtain and are suitable blood samples for primary health care centers.
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Effects of forest fragmentation on brown spider monkeys (Ateles hybridus) and red howler monkeys (Alouatta seniculus)Rimbach, Rebecca 04 September 2013 (has links)
No description available.
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Avaliacao do mecanismo de captacao e endocitose de crotoxina submetida a acao da radiacao gama, por macrofagos peritoneais de comundongosCARDI, BRUNO A. 09 October 2014 (has links)
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