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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Studies of Disulfide Bridge Formation in Human Carbonic Anhydrase Between Engineered Cysteines in Non Ideal Conformations Under Equilibrium and Kinetic Conditions / Studier av disulfidbryggebildning i humant karboanhydras mellan genom mutagenes införda cysteiner i icke-ideala konformationer vid jämvikts och kinetiska förhållandenMorssing Vilén, Eric January 2007 (has links)
<p>Stabilization of proteins is of great interest for the biotechnological society, industrial as well as research areas. Proteins with high stability are more suitable as reagents, easier to handle, store, transport and use in industrial processes. One way to stabilize a protein is to introduce a disulfide bridge into the structure by protein engineering. In this report the formation of a disulfide bridge between engineered cysteines in non ideal conformations in human carbonic anhydrase has been investigated. The disulfide bridge is not formed when the protein is in its native state. It is shown that when the protein is exposed to mild concentrations of urea in the presence of DTTox the disulfide bridge is formed. Also upon refolding in vitro, in a non oxidative environment, disulfide bridges are formed. This observation is worth to notice, since the disulfide bridge does not form to any appreciable extent when the protein is expressed and folded in vivo in Escherichia coli.</p>
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Studies of Disulfide Bridge Formation in Human Carbonic Anhydrase Between Engineered Cysteines in Non Ideal Conformations Under Equilibrium and Kinetic Conditions / Studier av disulfidbryggebildning i humant karboanhydras mellan genom mutagenes införda cysteiner i icke-ideala konformationer vid jämvikts och kinetiska förhållandenMorssing Vilén, Eric January 2007 (has links)
Stabilization of proteins is of great interest for the biotechnological society, industrial as well as research areas. Proteins with high stability are more suitable as reagents, easier to handle, store, transport and use in industrial processes. One way to stabilize a protein is to introduce a disulfide bridge into the structure by protein engineering. In this report the formation of a disulfide bridge between engineered cysteines in non ideal conformations in human carbonic anhydrase has been investigated. The disulfide bridge is not formed when the protein is in its native state. It is shown that when the protein is exposed to mild concentrations of urea in the presence of DTTox the disulfide bridge is formed. Also upon refolding in vitro, in a non oxidative environment, disulfide bridges are formed. This observation is worth to notice, since the disulfide bridge does not form to any appreciable extent when the protein is expressed and folded in vivo in Escherichia coli.
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Frequency changes and equilibria in experimental populations of Drosophila melanogaster with three lethal carrying fourth chromosomesSödergren, Agneta January 1979 (has links)
Populations where three different lethals are segregating as alleles have been analysed for the conditions of equilibrium and for the trends during elimination of one allele. Early and late selection as well as sexdependent and sexindependent selection has been taken into consideration.Cage populations of Drosophila melanogaster with different fourth chromosome lethals have been followed and compared to the theoretical model. When two marker chromosomes (ciDpol and spaCat) and one out of four recessive lethal chromosomes l(4)5, 1(4)8, 1(4)10 or l(4)14 were used, the same marker chromosome (ciDpol) became extinct in all populations. Early and late selective values which were obtained directly from the populations were compared to estimates of fitness components obtained in specially designed experiments of viability, developmental rate, mating ability and fecundity. When two out of the four recessive lethals and the marker chromosome, spaCat , were combined in new populations, all populations attained equilibrium withôut extinction. A correlation was found between the time of death of the lethal homozygotes and the equilibrium genotype frequencies. Overall selective values at equilibrium were estimated. / digitalisering@umu
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Market Integration Analysis and Time-series Econometrics: Conceptual Insights from Markov-switching Models / Marktintegrationsanalyse und Zeitreihenökonometrie: Begriffseinblicke aus den Markov-Switching ModellenAbunyuwah, Isaac 31 January 2008 (has links)
No description available.
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Stable isotope investigations on speleothems from different cave systems in Germany. / Stabile Isotopen-Untersuchungen an Speläothemen aus verschiedenen Höhlensystemen in Deutschland.Nordhoff, Peter 13 June 2005 (has links)
No description available.
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