This study aims to seek knowledge about what preschool teachers think about gender issues, and if it affects their work. The study has been performed in a multicultural district in Stockholm. A working party of three teachers, one male and two females, has been interviewed and observed. The empirical material from the interviews and observations has then been analyzed based on theories about gender, femaleness, maleness and cultural behaviour. The result proves that one of the three teachers considers that there are general typical female and male behaviour. One of the three teachers considers that there are differences in the children’s behaviour based on their gender. My observations prove that all the three teachers have some apprehensions about what is to be typical male or female behaviour. One of the three teachers thinks that the differences between her male and female colleagues are a result originating in culture and not gender. To sum up, my result suggests that the three teachers’ respective cultures, affects their idea of maleness and femaleness.