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The role of Perlecan in human cartilage developmentChuang, Christine Yu-Nung, Graduate School of Biomedical Engineering, Faculty of Engineering, UNSW January 2009 (has links)
Cartilage development relies on the coordinated presentation of biological signals to direct chondrocyte morphology and function. This is largely controlled by perlecan, a heparan sulfate proteoglycan (HSPG). Understanding the role of perlecan and its pendant glycosaminoglycan chains (GAG) in cartilage development is essential for advances in tissue engineered cartilage replacement strategies. Perlecan was immunolocalised to the pericellular matrix of prehypertrophic and hypertrophic chondrocytes in human fetal feet. Human fetal chondrocytes were isolated and cultured in 3-dimensional (3D) scaffolds for a period of 4 weeks. Their chondrogenic phenotype, based on extracellular matrix (ECM) components, was assessed and compared to 2D cultures. Chondrocyte perlecan was immunopurified from human fetal chondrocytes grown in vitro and fetal cartilage tissue and characterised using a combination of antibody-based techniques (ELISA, Western blotting) and gel electrophoresis. The biological function of chondrocyte perlecan was determined by its ability to form ternary complexes with fibroblast growth factors (FGF) and their receptors (FGFR) using an antibody-based technique as well as a cell proliferation assay using cells expressing FGFR isotypes. Perelcan was restricted to the prehypertrophic and hypertrophic zones of cartilage. This zonal organisation of chondrocytes and chondrogenic properties, determined by their morphology and PG deposition, was recapitulated in the 3D constructs while 2D cultures displayed dedifferentiated chondrocytes. Exogenous FGF2 promoted chondrocyte proliferation, while FGF18 stimulated the synthesis of perlecan, reflecting chondrocyte hypertrophy. Chondrocyte perlecan (630kDa) contained HS, chondroitin sulfate (CS) and keratan sulfate (KS) chains. Chondrocyte perlecan formed HS dependent ternary complexes with FGF2-FGFR1c and FGF18-FGFR3c, while FGF18-FGFR3c binding to perlecan protein core was also observed. Binding of FGF18-FGFR3c to chondrocyte perlecan HS was more promiscuous than FGF2-FGFR1c. Furthermore, chondrocyte perlecan HS mediated biological activity with FGF18 via FGFR3c, which was modulated by mammalian heparanase, while no biological activity was elicited by FGF2-FGFR1c. The findings underline how perlecan and its GAGs interact with FGF and FGFR in a spatio-temporal manner to promote signalling, effecting chondrocyte behaviour and morphology in cartilage development. This insight can be utilised in tissue engineering to improve the development of biologically functional cartilage replacements.
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The characterization of chicken and Drosophila Menin /Gianfelice, Gabriella Assunta. January 2006 (has links)
Thesis (M.Sc.)--York University, 2006. Graduate Programme in Biology. / Typescript. Includes bibliographical references (leaves 124-138). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:MR19747
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Perfusionskammersysteme zur Entwicklung organotypischer Kokulturen boviner Fibrozyten und Keratinozyten aus der KlaueHoffmann, Dirk. January 2006 (has links)
Freie Universiẗat, Diss., 2006--Berlin. / Dateiformat: zip, Dateien im PDF-Format.
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MT1-MMP in craniofacial development and FGF signalingChan, Kui-ming. January 2007 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2008. / Includes bibliographical references (leaf 153-171) Also available in print.
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Regulation of surfactant production by fetal type II pneumocytes and characterization of fibroblast-pneumocyte factor /Maker, Garth Lucas. January 2007 (has links)
Thesis (Ph.D)--Murdoch University, 2007. / Thesis submitted to the Faculty of Sustainability, Environmental and Life Sciences. Includes bibliographical references (leaves [134]-158).
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The role of sprouty-2 in the malignant transformation of human fibroblasts by HRAS oncogeneLito, Piro. January 2006 (has links)
Thesis (Ph. D.)--Michigan State University. Dept. of Biochemistry and Molecular Biology, 2006. / Title from PDF t.p. (viewed on Nov. 17, 2008) Includes bibliographical references. Also issued in print.
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The role of FGF signaling in retinal developmentHartge, Abbie A., January 2008 (has links) (PDF)
Thesis (M.S.)--University of Tennessee Health Science Center, 2008. / Title from title page screen (viewed on January 29, 2009). Research advisor: Dr. Michael A. Dyer, Ph.D. Document formatted into pages (vi, 47 p. : ill.). Vita. Abstract. Includes bibliographical references (p. 41-44).
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Einfluß von Calcitriol auf die Proliferation und Differenzierung muriner Keratinozyten in organotypischer Kultur und auf die Proliferation und Kontraktilität von Fibroblasten in Kollagengelen /Greiling, Doris. January 1994 (has links) (PDF)
Freie Univ., Diss.--Berlin, 1995.
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Fgf2-stimulated proliferation is lower in muscle precursor cells from old ratsJump, Seth, January 2009 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2009. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. "May 2009" Includes bibliographical references.
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FGF2 is weakly mitogenic for intimal smooth muscle cells : role of FGF receptor expression, cytoplasmic signaling and cell cycle regulation /Olson, Nels Eric. January 2000 (has links)
Thesis (Ph. D.)--University of Washington, 2000. / Vita. Includes bibliographical references (leaves 82-96).
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