Thesis (doctoral)--Technische Hochschule, Aachen, 2003.

Techn. Universiẗat, Diss., 2005--München.

Thesis (Ph. D.)--Ohio State University, 2006. / Available online via OhioLINK's ETD Center; full text release delayed at author's request until 2007 Mar 21

Thesis (Ph. D.)--Ohio State University, 2005. / Available online via OhioLINK's ETD Center; full text release delayed at author's request until 2006 May 17

Thesis (Ph.D.)--Kent State University, 2006. / Title from PDF t.p. (viewed Sept. 20, 2006). Advisor: Gary Meszaros. Keywords: cardiac fibroblasts; myofibroblasts; extracellular matrix; collagen VI; post-myocardial infarction remodeling. Includes bibliographical references (p. 135-152).

Thesis (Ph.D.)--York University, 2004. Graduate Programme in Biology. / Typescript. Includes bibliographical references. Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pNQ99209

Made available in DSpace on 2014-06-11T19:25:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-06-30Bitstream added on 2014-06-13T19:32:30Z : No. of bitstreams: 1 guerra_dm_me_botib.pdf: 2345678 bytes, checksum: 3a29cbd28a1d454f240afad94975996e (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A compreensão dos mecanismos moleculares controladores do desenvolvimento, função e regressão do CL bovino é necessária para o aprimoramento da manipulação hormonal ovariana. Fortes evidências sugerem o envolvimento de fatores de crescimento fibroblástico (FGFs) na regulação do crescimento e regressão do CL. “Splicing” alternativo de 4 genes formam sete subtipos de FGFRs com afinidade variável por diferentes FGFs. Os membros da subfamília do FGF8 (FGF8, 17 e 18) ativam eficientemente o FGFR3C e 4 e podem atuar em cooperação nos tecidos que expressão estes receptores. O objetivo deste trabalho foi determinar o padrão de expressão dos FGFRs e dos membros da subfamília do FGF8 no CL bovino (CL). Os CLs foram obtidos de ovários de abatedouro e classificados em 4 estádios de desenvolvimento (estádio/1= corpo hemorrágico, estádio/2= CL em desenvolvimento, estádio/3= CL maduro/início da luteólise funcional e estádio/4= luteólise estrutural). O RNAm foi mensurado por PCR semiquantitativo e a proteína localizada por imunohistoquímica. A expressão do RNAm codificante das isoformas ‘B’ e ‘C’ de FGFR1 e FGFR2 foi detectada no CL bovino por PCR associado à eletroforese e foi acompanhada pela localização da proteína nas pequenas e grandes células luteínicas. A expressão do RNAm do FGFR1C e 2C não variou durante o desenvolvimento luteínico, distintamente a expressão do FGFR1B aumentou no estádio 3. Embora os FGFRs 3B, 3C e 4 tenham sido detectados de forma inconsistente por PCR associado à eletroforese, o RNAm do FGFR3C e FGFR4 foram detectados por PCR em tempo real em todos os estádios do desenvolvimento luteínico. O RNAm do FGF18 foi detectado por PCR em tempo real em todos os estádios do desenvolvimento luteínico e sua abundancia do RNAm do FGF18 foi maior no estádio 3 comparado com os estádios 1, 2 e 4. Em contraste, os RNAm do FGF8 e 17... / The molecular mechanisms controlling the development, function and regression of the bovine corpus luteum are necessary for the improvement of reproductive biotechnologies. Strong evidence suggests the involvement of fibroblast growth factors (FGFs) in the regulation of growth, and regression of the corpus luteum (CL). Alternative splicing of 4 genes give rise to seven subtypes of FGFRs with varying affinity for different FGFs. FGF8 subfamily members (FGF8, 17 and 18) efficiently activate FGFR3C and FGFR4 and may act in cooperation in tissues expressing these receptors. The objective of the present study was to determine the pattern of expression of FGF8 subfamily members and FGFRs in the bovine CL. Bovine CLs were obtained from abattoir ovaries and classed into four stages of development (stage 1= corpus hemorragicum, stage 2= developing CL, stage 3= mature/early functional luteolysis CL, and stage 4= structural luteolysis). Expression of mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) followed by gel analysis (FGFR1-4) and real time RT-PCR (FGF8 subfamily members, FGFR3C and FGFR4) and proteins were localized by immunohistochemistry. Expression of mRNA encoding ‘B’ and ‘C’ spliced forms of FGFR1 and FGFR2 was readily detected in the bovine CL and was accompanied by isoform non-specific protein localization. FGFR1C and FGFR2C mRNA expression did not vary throughout CL lifespan, whereas FGFR1B was upregulated in the mature CL (stage III). FGFR3B, FGFR3C and FGFR4 expression was inconsistent in the bovine CL as assessed by PCR associated with gel analysis. FGF18, FGFR3C and FGFR4 mRNA was detected by real time PCR in all four developmental stages, and FGF18 mRNA abundance was higher in stage 3 (2.89  0.05; mean ± SEM) compared with stages 1 (0.3  0.27), 2 (0.56  1.27) and 4 (0.99  0.32). The m RNA expression ... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:28:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-03-01Bitstream added on 2014-06-13T18:07:24Z : No. of bitstreams: 1 silverio_kg_me_arafo.pdf: 1644294 bytes, checksum: 5d943591d5c51a3846c75163db9a2ebb (MD5) / O objetivo do presente estudo foi avaliar in vitro o efeito do condicionamento radicular com fator de crescimento de fibroblastos básico (b-FGF) sobre a morfologia e densidade de fibroblastos. Para tal, blocos de dentina com 4 mm2 de área de superfície foram obtidos de raízes de dentes humanos extraídos devido severo envolvimento periodontal, sendo instrumentados manualmente e autoclavados. Noventa amostras foram selecionadas aleatoriamente e distribuídas em 3 grupos segundo o tratamento de superfície prévio ao condicionamento com o b-FGF: sem tratamento - controle; 50 mg/mL de cloridrato de tetraciclina e EDTA a 24%. As 30 amostras de cada um destes 3 grupos foram distribuídas em 3 subgrupos quanto à dose de b-FGF: 0 æg/mL - controle; 50 æg/mL e 125 æg/mL. Após os tratamentos, as amostras foram incubadas a 37º C e 98% de umidade com 2mL de meio Eagle, sendo 1mL com fibroblastos de linhagem contínua (células McCoy) na concentração de 1 x 105 células/mL e 1mL meio sem células, por 24 h. Após as 24 h, as amostras foram submetidas a preparo de rotina para MEV e então, fotomicrografadas nos aumentos de 500X (densidade celular) e 1000X (morfologia celular). Em seguida, as fotomicrografias foram avaliadas por 3 examinadores treinados, calibrados, independentes e cegos, os quais verificaram morfologia e densidade celular segundo os escores propostos por Gamal et al. (1998) e Jenkins et al. (1988), respectivamente. A aplicação da Análise de Regressão pela Técnica da Árvore demonstrou haver diferenças estatisticamente significantes para a densidade celular (p<0,0001) entre os grupos EDTA, tetraciclina e controle, sendo que também houve diferenças entre as doses de 0/50 æg e 125 æg de b-FGF nas amostras condicionadas com EDTA (p<0,0001) e entre as doses de 0 e 50/125 æg de b-FGF nas amostras condicionadas com tetraciclina... . / The aim of this study was to evaluate in vitro the effect of the root surface conditioning with basic fibroblast growth factor (b-FGF) about morphology and density of fibroblasts. Dentin slices of with 4 mm2 of surface area were obtained from roots of teeth extracted due to severe periodontal involvment. These were scaled and sterilized. Ninety samples were randomly distributed into 3 groups according to treatment before application of b-FGF: non-treated - control; 50mg/mL of tetracycline HCl and EDTA 24%. The thirty samples of each group were distributed into 3 subgroups according to the concentration of b-FGF: 0 æg/mL - control; 50 æg/mL and 125 æg/mL. After treatments, the samples were incubated at 37ºC and 98% humidity with 1mL of Eagle Medium with 1 x 105 cells/mL of fibroblast from continuos lineage (McCoy Cells) plus 1mL this solution without cells during 24 hours. The samples were submitted to routine preparation for SEM and photographed at 500x (density celular) and 1000x (morphology celular). Three independent and blind examiners evaluated the fibroblast`s morphology and density, according to Gamam et al. (1998) and Jenkins et al. (1998), respectively. Classification and Regression Trees test results indicated significant differences on the density (p<0,0001) among EDTA, tetracycline and control groups with also differences between concentrations of 0/50æg and 125æg of b-FGF at the samples conditioning with EDTA (p<0,0001) and between concentrations of 0 and 50/125 æg of b-FGF at the samples conditioning with tetracycline(p<0,0001). The results of this test to morphology indicated significant differences between treatment or non-treatment with b-FGF, and that concentration of 125 æg demonstrated to be more favorable than the concentration of 50 æg. In conclusion, the treatment of root surfaces with b-FGF influenced the density... (Complete abstract, click electronic address below).

Atualmente é crescente o número de pacientes utilizando drogas que visam a alteração da remodelação óssea. Doenças como osteoporose e tumores ósseos têm possibilidade de tratamento com a utilização dos antirreabsortivos. Entretanto tais medicamentos apresentam, entre outros, um efeito colateral muito nocivo: a osteonecrose dos maxilares (ONM), que consiste em uma lesão rara, mas grave, da mandíbula ou maxila caracterizada por necrose óssea exposta. O denosumab é uma droga antirreabsortiva que possui um mecanismo de ação diferente do encontrado nos bisfosfonatos (BFs), medicação amplamente usada e anterior ao denosumab, entretanto já mostra efeitos colaterais similares aos BFs em relação à ONM e para ambos os medicamentos a fisiopatogenia da doença ainda não está esclarecida pela literatura Este trabalho teve como objetivo avaliar o efeito de diferentes concentrações do denosumab sobre a viabilidade, proliferação e migração de fibroblastos em cultura. Foram utilizados fibroblastos de mucosa bucal humana linhagem FMM1. Após serem submetidos aos testes de citotoxicidade com concentrações do denosumab variando de 10- 3?g a 10 - 7?g os fibroblastos não apresentaram quaisquer alterações quanto aos quesitos avaliados. Foi possível concluir que o denosumab não é citotóxico para fibroblastos em cultura. ecrose dos maxilares Fibroblastos / The number of patients using drugs that target the manipulation of bone remodeling is currently increasing. Bone volume diseases such as osteoporosis and tumors have the possibility of treatment with the use of antiresorptive medications. However, these drugs, among others, may present a very harmful side effect: osteonecrosis of the jaw (ONJ), which consists of a rare but severe injury characterized by exposed bone necrosis. The denosumab is an antiresorptive drug that presents a different mechanism of action found in bisphosphonates (BPs) and shows similar side effects to BPs regarding ONJ. BPs are a class of medication widely used and prior to denosumab. In both drugs the pathophysiology of the disease it is still not clear. This study aimed to evaluate the effect of denosumab in different concentrations on the viability, proliferation and migration of fibroblasts in culture. Were used human oral mucosa fibroblasts FMM1. After being subjected to denosumab concentrations ranging from 10-3?g to 10-7?g fibroblasts did not show any changes to the variables evaluated. It was possible to concluded that denosumab is not cytotoxic to fibroblasts in culture.

Cells have the ability to sense the rigidity of the extracellular matrix which directly affects the control of cellular functions in development, wound healing and malignant transformation. Polydimethylsiloxane elastomers are useful model biomaterials for mechanotransduction studies because they possess several advantages including ease of fabrication, tunable elasticity and modifiable surface chemistry. In this work, we are investigating the influence of matrix stiffness on adhesion strength and the mechanosensory structures that regulate these processes. In addition, the effect of surface modifications to this elastic substrate system on other physical properties such as local stiffness and topography will be analyzed. Based on previous research, we hypothesized that cell adhesion dependent processes will be regulated by matrix stiffness, but that surface chemistry influences on protein adsorption could provide overriding regulatory signals. The results of this research will provide insight into the interconnected processes of mechanosensing and cell adhesion strengthening, and reveal criteria for designing instructive biomaterials with specific mechanical and chemical properties.