La gestion des eaux pluviales urbaines connait une évolution majeure depuis les années 1960 : d'une stratégie basée sur l'évacuation rapide et totale des eaux pluviales de la ville, on passe progressivement à une stratégie de contrôle à la source (CS). Ces 10 dernières années ont vu une généralisation du CS, ainsi qu'en témoigne l'augmentation du nombre de réglementations de rejet. Ces dernières imposent, dans les nouvelles parcelles urbanisées, la réalisation d'ouvrages ayant pour fonction de réguler et/ou de retenir les eaux pluviales : les techniques alternatives (TA). Une systématisation de ces réglementations ne va pas manquer de générer dans l'avenir de nombreux bassins versants urbains fortement équipés en TA, et dont le comportement hydrologique sera déterminé par les réglementations appliquées. Il apparaît que le choix d'une réglementation par une collectivité est un choix complexe et, d'une collectivité à l'autre, des logiques différentes sont appliquées, dont la cohérence globale peut être discutée. Cette thèse questionne et analyse les politiques de CS, et en particulier les réglementations de rejet, dans une perspective de compréhension de leurs effets hydrologiques dans le long terme. Elle comprend deux analyses complémentaires : nous étudions d'abord l'évolution des politiques de CS dans six collectivités en France, afin de déterminer les logiques appliquées dans le choix des réglementations. Nous présentons ensuite une analyse hydrologique des conséquences de ces réglementations sur le comportement de bassins versant urbains "modèles". Cette analyse, centrée sur la modélisation hydrologique des bassins, permet de questionner les politiques de CS actuelles, mais aussi de discuter des outils que l'hydrologie urbaine peut fournir pour permettre la mise en place de politiques de CS cohérentes, en phase avec le fonctionnement hydrologique des bassins versants / Urban stormwater management is facing a major evolution since 1960: from a strategy based on the complete and fast conveyance of stormwater outside of the urban area, to a strategy based on source control (SC). Recently, SC is becoming a common practice, in connection with the increasing diffusion of flow regulations. These regulations prescribe, for all new urban development, to build facilities (Best Management Practices, BMP) to reduce the flow-rate or the volume of stormwater entering the sewer system. A wide application of flow regulations will determine the future hydrological behaviour of many urban catchments. The choice of a regulation is a complex task for local authorities, involving several logics, whose global coherence can be discussed. This thesis analyses SC policies and regulations and discusses them in terms of their long-term hydrological effects. Two complementary approaches are developed. The first is based on the analysis of the evolution of SC policies in six French authorities, and it aims to define and discuss the logics applied. The second consists in the hydrological modelling of the consequences of SC regulations on the behaviour of two experimental catchments. This hydrological analysis allows (i) to evaluate the pertinence of actual SC policies and (ii) to discuss which tools urban hydrology can provide to support the development of coherent SC policies, according to the hydrological functioning of urban catchments

Functional hyperaemia is an important mechanism by which increased neuronal activity is matched by a rapid and regional increase in blood supply. This mechanism is facilitated by a process known as “neurovascular coupling” – the orchestrated communication system involving the cells that comprise the neurovascular unit (neurons, astrocytes and the smooth muscle and endothelial cells lining arterioles). Blood flow regulation and neurovascular coupling are altered in several pathological states including hypertension, diabetes, Alzheimer’s disease, cortical spreading depression and stroke. By adapting and extending other models found in the literature, we create, for the first time, a mathematical model of the entire neurovascular unit that is capable of simulating two separate neurovascular coupling mechanisms: a potassium- and EET-based and a NO-based mechanism. These models successfully account for several observations seen in experiment. The potassium/EET-based mechanism can achieve arteriolar dilations similar in magnitude (3%) to those observed during a 60-second neuronal activation (modelled as a release of potassium and glutamate into the synaptic cleft). This model also successfully emulates the paradoxical experimental finding that vasoconstriction follows vasodilation when the astrocytic calcium concentration (or perivascular potassium concentration) is increased further. We suggest that the interaction of the changing smooth muscle cell membrane potential and the changing potassium-dependent resting potential of the inwardly rectifying potassium channel are responsible for this effect. Furthermore, our simulations demonstrate that the arteriolar behaviour is profoundly affected by depolarization of the astrocytic cell membrane, and by changes in the rate of perivascular potassium clearance or the volume ratio between the perivascular space and astrocyte. In the modelled NO-based neurovascular coupling mechanism, NO exerts its vasodilatory effects via neuronal and endothelial cell sources. With both sources included, the model achieves a 1% dilation due to a 60-second neuronal activation. When the endothelial contribution to NO production is omitted, the arteriole is more constricted at baseline. Without the endothelial NO contribution, the arteriolar change in diameter during neuronal activity is greater (6%). We hypothesize that NO has a dual purpose in neurovascular coupling: 1) it dixxxvi rectly mediates neurovascular coupling through release by neuronal sources, and 2) it indirectly modulates the size of the neurovascular coupling response by determining the baseline tone. Our physiological models of neurovascular coupling have allowed us to replicate, and explain, some of the phenomena seen in both neurovascular coupling-oriented and clinicallyoriented experimental research. This project highlights the fact that physiological modelling can be used as a tool to understand biological processes in a way that physical experiment cannot always do, and most importantly, can help to elucidate the cellular processes that induce or accompany our most debilitating diseases.

Cette recherche évalue le degré de régulation hydrologique induite par le barrage-réservoir Paso de las Piedras sur la Sauce Grande et quantifie les effets de cette régulation sur la hydrologie, la morphologie et la température de l'eau de la rivière en aval. Le travail de thèse consiste en une évaluation exhaustive et systématique des impacts du barrage-réservoir sur le système fluvial basée sur la compréhension des processus naturels qui se produisent en amont. En plus de fournir des informations sur les impacts hydrologiques, morphologiques et thermiques de la construction du barrage Paso de las Piedras et de l'exploitation de son réservoir, cette étude génère des séries de données climatiques et hydrologiques qui fournissent une base significative sur laquelle fonder des recherches futures. Par ailleurs, cette étude met en place un cadre méthodologique appliqué à l'analyse hydrogéomorphologique des bassins versants non jaugés qui a un grand potentiel d'application dans d'autres bassins de la région similaires ainsi que dans d'autres régions semi-arides du monde. / Despite the regional importance of the Sauce Grande River as main source for water supply and the large capacity of the Paso de las Piedras Reservoir, both the hydrology of the river basin and the effects of the impoundment on the river environment remain poorly evaluated. This study provides the very first assessment of the degree of flow regulation induced by the Paso de las Piedras Dam on the middle section of the Sauce Grande River, and quantifies its impacts on the hydrology, morphology and patterns of water temperature of the river downstream from the impoundment. In addition to providing new information on the response of regulated rivers to upstream impoundment and on the effects of impoundment on the Sauce Grande River specifically, this study generates spatial, climatic and hydrologic data and implements a methodological framework to hydrological assessment of ungauged basins.

Background Abnormal renal hemodynamic responses to salt-loading are thought to contribute to salt-sensitive (SS) hypertension. However, this is based largely on studies in anesthetized animals, and little data are available in conscious SS and salt-resistant rats. Methods and Results We assessed arterial blood pressure, renal function, and renal blood flow during administration of a 0.4% NaCl and a high-salt (4.0% NaCl) diet in conscious, chronically instrumented 10- to 14-week-old Dahl SS and consomic SS rats in which chromosome 1 from the salt-resistant Brown-Norway strain was introgressed into the genome of the SS strain (SS.BN1). Three weeks of high salt intake significantly increased blood pressure (20%) and exacerbated renal injury in SS rats. In contrast, the increase in blood pressure (5%) was similarly attenuated in Brown-Norway and SS.BN1 rats, and both strains were completely protected against renal injury. In SS.BN1 rats, 1 week of high salt intake was associated with a significant decrease in renal vascular resistance (-8%) and increase in renal blood flow (15%). In contrast, renal vascular resistance failed to decrease, and renal blood flow remained unchanged in SS rats during high salt intake. Finally, urinary sodium excretion and glomerular filtration rate were similar between SS and SS.BN1 rats during 0.4% NaCl and high salt intake. Conclusions Our data support the concept that renal vasodysfunction contributes to blood pressure salt sensitivity in Dahl SS rats, and that genes on rat chromosome 1 play a major role in modulating renal hemodynamic responses to salt loading and salt-induced hypertension.