Thesis (M.S.)--Rhodes University, 2003. / Title from PDF t.p. (viewed on June 9, 2006). Includes bibliographical references (p. 110-122).

Thesis (MSc)--University of Stellenbosch, 2005. / ENGLISH ABSTRACT: The Mountain Fynbos biome, a division of the Cape Floristic Region (CFR), is home to round-leafed Buchu [Agathosma betulina (Berg.) Pillans], one of South Africa’s best-known endangered herbal medicinal plants. Agathosma betulina is renowned as a traditional additive to brandy or tea, which is used for the treatment of a myriad of ailments. In its natural habitat, A. betulina thrives on mountain slopes in acid and highly leached gravelly soils, with a low base saturation and low concentrations of organic matter. To adapt to such adverse conditions, these plants have formed mutualistic symbioses with arbuscular mycorrhizal (AM) fungi. In this study, the effect of indigenous AM taxa on the physiology of A. betulina is investigated. In addition, the AM taxa responsible for these physiological responses in the plant were identified using morphological and molecular techniques. Agathosma betulina was grown under glasshouse conditions in its native rhizosphere soil containing a mixed population of AM fungi. Control plants, grown in the absence of AM fungi, were included in the experimentation. In a time-course study, relative growth rate (RGR), phosphorus (P)-uptake, P utilization cost, and carbon (C)-economy of the AM symbiosis were calculated. The data showed that the initial stages of growth were characterized by a progressive increase in AM colonization. This resulted in an enhanced P-uptake in relation to non-AM plants once the symbiosis was established. Consequently, the lower P utilization cost in AM plants indicated that these plants were more efficient in acquiring P than non-AM plants. When colonization levels peaked, AM plants had consistently higher growth respiration. This indicated that the symbiosis was resulting in a C-cost to the host plant, characterized by a lower RGR in AM plants compared to non-AM plants. Arbuscular mycorrhizal colonization decreased with increasing plant age that coincided with a decline in P-uptake and growth respiration, along with increases in RGR to a level equal to non-AM plants. Consequently, the AM benefit was only observed during the initial stages of growth. In order to identify the AM fungi in planta, morphological and molecular techniques were employed, which indicated colonization by AM fungi belonging to the genera Acaulospora and Glomus. Phylogenetic analyses of a dataset containing aligned 5.8S ribosomal RNA gene sequences from all families within the Glomeromycota, including sequences obtained during the study, supported the above mentioned identification. / AFRIKAANSE OPSOMMING: Die Fynbos bergbioom, ‘n onderafdeling van die Kaapse Floristiese Streek, huisves rondeblaar Boegoe [Agathosma betulina (Berg.) Pillans], een van Suid Afrika se bekendste bedreigde medisinale plante. Agathosma betulina is bekend vir sy gebruik as tinktuur vir die behandeling van verskeie kwale. Die plant kom voor in bergagtige streke, in suur en mineraal-arm grond, met ‘n lae organiese inhoud. Gevolglik, om aan te pas by hierdie ongunstige kondisies, vorm die plante simbiotiese assosiasies met blaasagtige, struikvormige mikorrisa (BSM). In die huidige studie is die effek van hierdie BSM op die fisiologie van A. betulina ondersoek. Die identiteit van die BSM is ook gevolglik met morfologiese en molekulêre identifikasie tegnieke bepaal. Agathosma betulina plante is onder glashuis kondisies in hul natuurlike grond gekweek, wat ‘n natuurlike populasie van BSM bevat het. Kontroles is ook in die eksperiment ingesluit en hierdie stel plante is met geen BSM geïnokuleer nie. Gevolglik is die relatiewe groeitempo, fosfor opname, fosfor verbuikerskoste asook die koolstof ekonomie van die plante bereken. Die data het getoon dat die eerste groeifase gekarakteriseer is deur toenames in BSM kolonisasie vlakke. Dit het tot ‘n hoër fosfor opname in BSM geïnokuleerde plante gelei. Die laer fosfor verbuikerskoste gedurende hierdie fase het aangedui dat die plante wat geïnokuleer is met BSM oor beter meganismes beskik het om fosfor uit die grond te bekom. Toe BSM kolonisasie vlakke gepiek het, was groei respirasie hoër in BSM geïnokuleerde plante as in die kontroles. Dit het aangedui dat die BSM kolonisasie van plante tot hoër koolstof kostes vir hierdie plante gelei het, wat weerspieël is in die laer groeitempo van die BSM geïnokuleerde plante. Die BSM kolonisasie vlakke het gedaal met toenemende ouderdom van hul gasheer plante, wat gekarakteriseer is deur ‘n laer opname van fosfor en laer groei respirasie, tesame met ‘n toename in relatiewe groeitempo tot vlakke soortgelyk aan die van die kontrole plante. Die BSM voordele vir die plant is dus net gedurende die eerste groeifase waargeneem. Die BSM wat verantwoordelik is vir hierdie fisiologiese veranderinge is gevolglik geïdentifiseer met behulp van morfologiese en molekulêre tegnieke en dit is gevind dat BSM wat behoort tot die genera Acaulospora en Glomus binne hierdie plante voorkom. Filogenetiese analise gegrond op opgelynde 5.8S ribosomale RNA geen volgordes afkomstig van al die families binne Glomeromycota asook volgordes gevind in die studie, het die bogenoemde identifikasie gestaaf.

Thesis submitted in fulfilment of the requirements for the degree Master of Technology: Horticulture in the Faculty of Applied Sciences at the Cape Peninsula University of Technology, 2013 / Cape Flats Sand Fynbos (CFSF) is one of the most poorly conserved vegetation types in the Cape Floral Kingdom, and a large proportion of unconserved land is degraded, primarily as a result of transformation by agricultural, urban developments and invasion by alien plants. Fynbos restoration is one of the most important management interventions, both within the current conservation areas and in any future land remnants acquired for conservation. Many extant remnants are fragmented and isolated, and if successful restoration protocols are found, it may be possible to improve the conservation targets for this critically endangered vegetation type. On old fields, where indigenous soil seed banks have been lost due to alien plant invasion and anthropogenic action, it is essential to reintroduce the longer-lived fynbos components that contribute to vegetation structure, in order to facilitate the progress of the ecosystem on a more natural trajectory. This research is built on an earlier study of optimal ground-preparation treatments for restoring Sand Fynbos to old fields. This former study indicated that fossorial mammals (molerats and gerbils) may occur in dense colonies on old fields and present an obstacle to successful seedling establishment. The project aims to provide protocols for the establishment of indigenous seedlings from harvested seed onto old fields, in order to restore Sand Fynbos vegetation. Different pre-sowing treatments and sowing techniques were tested on large field plots to determine the most efficient protocol. The objectives of the research were: a) to investigate optimal pre-sowing treatments of indigenous seed for restoring degraded Sand Fynbos vegetation in old field sites; b) to investigate optimal sowing techniques on large field plots for restoring degraded Sand Fynbos vegetation in old field sites with depleted indigenous seed banks; c) to provide guidelines and disseminate information on optimal sowing protocols, and their costs, for restoring Sand Fynbos vegetation in degraded areas and old fields. A further component of the research was to calculate the costs of all treatments on a per hectare basis in order to assess the cost-effectiveness of the different options. Several different seed treatments may potentially increase the germination rate and promote fynbos restoration. These are scarifying, smoke, smoke water, chemical, light and temperature pre-sowing treatments. In order to keep the number of treatments (including their interactions) to a manageable level, only soaking in smoke water extract and seed coat scarification with course sand and grit were tested. A seed sample of each species was x-rayed at the Millennium Seed Bank in the United Kingdom, to test for viability in the seed samples, 52 % of the seed collected were empty, a typical indication of wild harvested seed. All species were germinated at 10/25 °C and 16/8 hours light/dark respectively. Scarification had a larger overall germination success, smoke water had very little effect on CFSF species, it is rather that germination is related to temperatures during a fire that result in seed coat splitting. It was recommended that further investigation using more species across the Sand Fynbos vegetation be conducted on pre-germination effects of heat and scarification. The study site had been cleared of woody invasive alien vegetation and additional site preparation included the application of a systemic herbicide to kill undesirable herbaceous weeds, prescribed fire to clear the site of woody debris and destroy weed seeds, and the local control of fossorial mammals (gerbils and molerats) by placing raptor perches and owl nesting boxes around the site. This research found that the use of herbicide shortly after the prescribed fire and once again prior to sowing was successful in controlling herbaceous weeds and the indigenous grass Cynodon dactylon. The challenge to using prescribed burning on old fields was low fuel loads, which resulted in a cool patchy. It is suggested that cutting and spreading of alien plant biomass is tested as a solution, however, the material must be evenly spread across the site and not stacked into piles which can cause excessively hot fires and scorching of the soil. In order to better understand soil conditions across the site, soil samples were collected prior to sowing, to analyse for soil macronutrients, organic matter and pH. It was established that all the excess nutrients added to the site from agriculture and pasturage over the years had leached from the soil. However, the organic matter content of the soil was extremely low and research needs to be done on the organic carbon content of the soils, how these relate to soil micro biota (which species are present and their relationship with CFSF species) and how best to enrich the site with humic matter for restoration and establishment of Cape Flats Sand Fynbos. The field trial was set up in the Blaauwberg Nature Reserve, a random split-plot block design, was replicated five times and used to investigate the selected seedbed preparation and sowing techniques, namely: broadcast sowing onto unprepared seedbed, broadcast with plank embedding of seed onto disked seedbed, broadcast onto disked seedbed and hydro-seed with disked seedbed. Results from the research found that the most successful methods for sowing seeds were the hydro-seeding and broadcast with plank embed. These methods may have provided better contact between the soil and seeds and better protection from predation and wind. Economically the broadcast and embed was better as machinery was more efficient and effective than manual labour. This study recommended that these two methods be combined with the additional planting of rare and threatened species in clumps to determine the benefits and interactions of each technique over the long term.

Thesis (MSc (Microbiology))--Stellenbosch University, 2008. / The genus Fusarium contains members that are phytopathogens of a number of agricultural commodities causing severe diseases such as wilts and rots. Fusarium species also secrete mycotoxins that have devastating effects on humans and animals. The ability of Fusarium species to change their genetic makeup in response to their immediate environment allows these fungi to exist in diverse habitats. Due to the ubiquitous nature of Fusarium, it forms part of the fungal communities in both agricultural and native soils. Fynbos is the major vegetation type of the Cape Floristic Region (CFR), which is a region that is renowned for its high plant species diversity and endemism. In this study, the occurrence and distribution of Fusarium species in indigenous fynbos soils and associated plant debris is investigated. In addition, the phylogenetic relationships between Fusarium species occurring in this particular habitat are evaluated. Fusarium isolates were recovered from soils and associated plant debris, and identified based on morphological characteristics. The morphological identification of isolates was confirmed using Polymerase Chain Reaction (PCR) based restriction fragment length polymorphism (RFLP) analyses of the translation elongation factor 1 alpha (TEF-1α) and internal transcribed spacer (ITS) regions. Furthermore, phylogenetic relationships between Fusarium species were based on the TEF-1α, ITS and β-tubulin gene regions. One-hundred-and-twenty-two (122) Fusarium strains were isolated from the fynbos soils in the Cape Peninsula area (Western Cape). Based on both morphological and molecular identification, the most prevalent Fusarium species in the fynbos soils were F. oxysporum Schlecht. emend. Snyd. and Hans., F. solani (Martius) Appel and Wollenw. emend. Snyd. and Hans., F. equiseti (Corda) Sacc. and an undescribed Fusarium species. Fusarium oxysporum was the dominant species in fynbos soils and strains of this species displayed significant genetic variability. Some strains of both F. oxysporum and F. solani showed close phylogenetic affinities to formae speciales (strains pathogenic to specific plant hosts) in the phylogenetic analyses. However, no diseased plants were observed in and within the vicinity of our sampling sites. In the third chapter, the undescribed Fusarium strains are described as Fusarium peninsulae prov. nom. Morphologically these strains are characterized by falcate macroconidia produced from brown sporodochia. The macroconidia are pedicellate, falcate to curved with hooked apical cells. Also, this fungus produces apedicellate mesoconidia on polyphialides in the aerial mycelium and forms microconidia sparsely. Chlamydospores are formed abundantly on aerial mycelium and submerged hyphae. All these morphological characteristics closely relate this fungus to F. camptoceras species complex in Fusarium section Arthrosporiella. However, phylogenetic analysis based on the ITS sequences differentiate these strains from F. camptoceras and other related species in section Arthrosporiella. Considering the fact that both as phytopathogens and saprophytic fungi, Fusarium species secrete a variety of cell wall degrading enzymes such as cellulases and xylanases. These enzymes allow the fungi to degrade the plant cell wall components to obtain nutrients. In Fusarium, notably endoxylanases play a role in phytopathogenesis of these fungi. Endoxylanase enzymes from F. oxysporum f. sp. lycopersici, F. verticillioides and F. graminearum have been characterized. In this final chapter, the use of the endoxylanase encoding gene, as a molecular marker in phylogenetic analysis was evaluated using F. graminearum (Fg) clade species as model. Degenerated primers were designed and the endoxylanase region amplified by PCR, cloned and sequenced. PAUPgenerated neighbour-joining analysis of the endoxylanase (XYL) region enabled all species to be distinguished and was as informative as the analysis generated with UTPammonia ligase (URA), phosphate permase (PHO), reductase (RED) and trichothecene 3- О-acetyltransferase (TRI101). Furthermore, the results of the phylogenetic analysis of XYL showed better species resolution in comparison to the analysis of the structural genes (TEF-1α and histone H3). Overall, the results demonstrated that phylogenetic analysis of XYL combined with other functional genes (URA, PHO, RED and TRI101) clearly distinguished between the Fg clade species far better than the analysis of structural genes (TEF-1α and histone H3).

Thesis (PhD)--Stellenbosch University, 2012. / Please refer to full text for abstract.

A research report submitted to the Faculty of Science, University of the Witwatersrand, in partial fulfillment of the MSc by Coursework Degree (in Resource Conservation Biology) June 2017. South Africa. / Understanding why species occur where they do and, predicting where species might migrate to under different global change scenarios is an important aspect of biodiversity conservation. Regions that harbour high levels of species diversity and endemism arising from sharp local climatic and ecological gradients are highly susceptible to changing conditions. Kumara plicatilis is a tree aloe endemic to the Boland mountain ranges in the species-rich fynbos region in the Western Cape Province of South Africa. The species is currently listed as Least Concern as far as habitat degradation, population decline, invasive species and direct-use threats are concerned although impacts of anthropogenic climate change on this habitat specialist remain undocumented. This study used species distribution models to successfully classify the environmental niche of the species as well as delineate spatial patterns of probable occurrence and abundance based on this niche. In addition, models based on the IPCCs 2014 ‘best-case’ and ‘worst-case’ climate change scenarios provide projections of changes in the spatial occurrence patterns of Kumara plicatilis expected under conditions of shifting climates. Niche-based statistical analyses were further used to draw temporal comparisons between current and future projected ranges to ascertain the degree and properties of shared niche space now and in the future. Results indicate that suitable habitat conditions for the species distribution is irregularly spread around the central and southwestern fynbos region constrained by several climatic and biophysical variables including winter rainfall and temperature conditions as well as vegetation type. The species is expected to experience limited to severe declines in the area of suitable habitat available under mild and harsh climate change conditions, respectively. The patterns arising from these models are in line with the environmental niche measurements which show large degrees of overlap between current and future niche space of the species. These outcomes suggest that Kumara plicatilis displays traits of environmental niche conservatism where unsuitable climate and biophysical conditions can limit its geographic range and local extinction of populations can occur due to global change. Whilst the results of this study offer a useful and initial insight into the possible impacts of shifting climates on this species, outcomes from modeling should be interpreted with caution to reach the best management decisions and conservation action for this endemic species. / MT 2017

Increasing urbanisation is one of the biggest pressures to vegetation in the City of Cape Town. The growth of the city dramatically reduced the area under indigenous Fynbos vegetation, which remains in isolated fragments. These are subject to a number of threats including atmospheric deposition, atypical fire cycles and invasion by exotic plant and animal species. Especially the Port Jackson willow (Acacia saligna) extensively suppresses the indigenous Fynbos vegetation with its rapid growth. The main objective of this study was to investigate indicators for a quick and early prediction of the health of the remaining Fynbos fragments in the City of Cape Town with help of remote sensing. First, the productivity of the vegetation in response to rainfall was determined. For this purpose, the Enhanced Vegetation Index (EVI), derived from Terra MODIS data with a spatial resolution of 250m, and precipitation data of 19 rainfall stations for the period from 2000 till 2008 were used. Within the scope of a flexible regression between the EVI data and the precipitation data, different lags of the vegetation response to rainfall were analysed. Furthermore, residual trends (RESTREND) were calculated, which result from the difference between observed EVI and the one predicted by precipitation. Negative trends may suggest a degradation of the habitats. In addition, the so-called Rain-use Efficiency (RUE) was tested in this context. It is defined as the ratio between net primary production (NPP) – represented by the annual sum of EVI – and the annual rainfall sum. These indicators were analysed for their suitability to determine the health of the indigenous Fynbos vegetation. Furthermore, the degree of dispersal of invasive species especially the Acacia saligna was investigated. With the specific characteristics of the tested indicators and the spectral signature of Acacia saligna, i.e. its unique reflectance over the course of the year, the dispersal was estimated. Since the growth of invasive species dramatically reduces the biodiversity of the fragments, their presence is an important factor for the condition of ecosystem health. This work focused on 11 test sites with an average size of 200ha, distributed over the whole area of the City of Cape Town. Five of these fragments are under conservation and the others shall be protected in the near future, too, which makes them of special interest. In January 2010, fieldwork was undertaken in order to investigate the state and composition of the local vegetation. The results show promising indicators for the assessment of ecosystem health. The coefficients of determination of the EVI-rainfall regression for Fynbos are minor, because the reaction of this vegetation type to rainfall is considerably lower than the one of the invasive species. Thus, a good distinction between indigenous and alien vegetation is possible on the basis of this regression. On the other hand, the RESTREND method, for which the regression forms the basis, is only of limited use, since the significance of these trends is not given for Fynbos vegetation. Furthermore, the RUE has considerable potential for the assessment of ecosystem health in the study area. The Port Jackson willow has an explicitly higher EVI than the Fynbos vegetation and thus its RUE is more efficient for a similar amount of rainfall. However, it has to be used with caution, because local and temporal variability cannot be extinguished in the study area over the rather short MODIS time series. These results display that the interpretation of the indicators has to be conducted differently from the literature, because the element of invasive species was not considered in most of the previous papers. An increase in productivity is not necessarily equivalent with an improvement in health of the fragment, but can indicate a dispersal of Acacia saligna. This shows the general problem of the term ‘degradation’ which in most publications so far is only measured by productivity and other factors like invasive species are disregarded. On the basis of the EVI-rainfall regression and statistical measures of the EVI, the distribution of invasive species could be delineated. Generally, a strong invasion of the Port Jackson willow was discovered on the test sites. The results display that a reasoned and sustainable management of the fragments is essential in order to prevent the suppression of the indigenous Fynbos vegetation by Acacia saligna. For this purpose, remote sensing can give an indication which areas changed so that specific field surveys can be undertaken and subsequent management measures can be determined. / Zunehmende Urbanisierung stellt eine der größten Bedrohungen für die Vegetation im Großraum Kapstadt dar. Durch das schnelle Wachstum der Stadt bleibt immer weniger der ursprünglichen Vegetation in isolierten Fragmenten zurück. Diese sind in ihrer Funktion als Lebensraum für Flora und Fauna unter Anderem durch Luftverschmutzung, untypische Feuerzyklen und das Eindringen fremder Arten gefährdet. Besonders die Weidenblatt-Akazie (Acacia Saligna) verdrängt die einheimische Fynbos-Vegetation großflächig durch ihr schnelles Wachstum. Hauptziel dieser Arbeit war es, mit Hilfe der Fernerkundung Indikatoren zu finden, um eine schnelle und frühzeitige Aussage über die Gesundheit der verbliebenen natürlichen Vegetationsfragmente im Großraum Kapstadt zu ermöglichen. Zunächst wurde die Produktivität der Vegetation und deren Reaktion auf Niederschlag analysiert. Zu diesem Zweck wurden der Enhanced Vegetation Index (EVI) aus Terra-MODIS-Daten mit einer räumlichen Auflösung von 250m und Niederschlagsdaten von 19 Wetterstationen aus dem Zeitraum 2000 bis 2008 verwendet. Im Rahmen einer flexiblen Regression zwischen EVI und Niederschlagsdaten wurden verschiedene Verzögerungen der Reaktion der Vegetation auf den Niederschlag getestet. Des Weiteren wurden residuale Trends (RESTREND) berechnet, die sich aus der Differenz zwischen beobachtetem EVI und dem aus dem Niederschlag vorhergesagten EVI ergeben. Zusätzlich wurde die sogenannte Rain-use Efficiency (RUE) getestet. Diese ist definiert durch das Verhältnis zwischen Nettoprimärproduktion, repräsentiert durch die Jahressumme des EVI, und der Jahressumme des Niederschlags. Die angewandten Indikatoren wurden darauf untersucht, ob sie eine Aussage über die Gesundheit der einheimischen Fynbos-Vegetation ermöglichen. Des Weiteren wurde der Verbreitungsgrad invasiver Arten, besonders der der Weidenblatt-Akazie bestimmt. Auf Basis der spezifischen Charakteristika der getesteten Indikatoren und der spektralen Signatur von Acacia saligna, also ihrer besonderen Reflexion über den Jahresverlauf, wurde die Verbreitung ermittelt. Da das ungehinderte Wachstum invasiver Arten die Biodiversität der Fragmente stark verringert, ist ihre Anwesenheit ein wichtiger Faktor für die Gesundheit von Ökosystemen. Diese Arbeit konzentrierte sich auf 11 Testflächen mit einer durchschnittlichen Größe von 200ha, die über die gesamte Fläche des Großraums Kapstadt verteilt sind. Fünf dieser Fragmente stehen bereits unter Schutz, während die anderen in absehbarer Zeit folgen sollen; dies macht sie von besonderem Interesse. Im Januar 2010 wurden Geländearbeiten durchgeführt um den Zustand und die Zusammensetzung der Vegetation vor Ort festzustellen. Die Ergebnisse weisen aussichtsreiche Indikatoren zur Abschätzung der Ökosystemgesundheit auf. Die Werte des Bestimmtheitsmaßes der EVI-Niederschlags-Regression sind niedrig für Fynbos, da die Reaktion dieses Vegetationstyps auf Niederschlag wesentlich geringer ist als die der invasiven Arten. Daher ist auf Basis dieser Regression eine gute Unterscheidung zwischen einheimischer und invasiver Vegetation möglich. Auf der anderen Seite ist die RESTREND-Methode, für die diese Regression die Grundlage bildet, nur begrenzt von Nutzen, da die Signifikanzen dieser Trends für Fynbos-Vegetation nicht gegeben sind. Des Weiteren weist die RUE Potential für die Abschätzung von Ökosystemgesundheit im Testgebiet auf. Die Weidenblatt-Akazie hat einen wesentlichen höheren EVI als die Fynbos-Vegetation und daher ist deren RUE bei vergleichbarer Niederschlagsmenge effizienter. Dennoch muss diese mit Vorsicht angewandt werden, da die hohe lokale und temporale Variabilität der RUE im Testgebiet über die relativ kurze MODIS-Zeitserie nicht eliminiert werden kann. Die Ergebnisse verdeutlichen zudem, dass die Interpretation der Indikatoren anders als in der Literatur durchgeführt werden muss, da das Element der invasiven Vegetation in den meisten der vorangegangenen Arbeiten nicht berücksichtigt wurde. Ein Anstieg der Produktivität ist hier nicht gleichzusetzen mit einer Verbesserung der Gesundheit eines Fragments, sondern deutet viel mehr auf eine Verbreitung der Weidenblatt-Akazie hin. Dies verdeutlicht das generelle Problem des Begriffs ‚Degradation‘, welche in den meisten Veröffentlichungen nur über die Produktivität der Vegetation bestimmt wird während andere Faktoren wie zum Beispiel invasive Arten unberücksichtigt bleiben. Auf Basis der EVI-Niederschlags-Regression und der statistischen Messgrößen des EVI konnte die Verbreitung der invasiven Arten abgegrenzt werden. Generell wurde ein starker Befall der Testflächen durch die Weidenblatt-Akazie festgestellt. Die Ergebnisse machen deutlich, dass ein durchdachtes und nachhaltiges Management der Fragmente notwendig ist um die Verdrängung der einheimischen Fynbos-Vegetation durch Acacia saligna zu verhindern. Die Fernerkundung kann zu diesem Zweck Hinweise liefern, welche Flächen sich verändert haben um anschließend gezielte Begehungen vorzunehmen und Maßnahmen einzuleiten.

Thesis (MSc)--University of Stellenbosch, 2008. / Bibliography. Also available via the Internet.

Thesis (DTech. degree in Crop Sciences) -- Tshwane University of Technology, 2011. / The genus Psoralea (tribe Psoraleae, family Leguminosae) is indigenous to the Cape Fynbos of South Africa and consists of 50 species that occupy different habitats, ranging from well-drained upland soils to creeks and permanent wetlands. However, little is known about their symbiosis, associated microsymbionts and or adaptation to the nutrient-poor, sandy, acidic soils of the Cape Fynbos. This study is the first to report the presence of calcium oxalate crystals in the outer cortex of P. pinnata and the occurrence of alkali and rare earth elements such as Sr, Rb, Zr and Y in tissue components of N2-fixing nodules (with unknown roles/functions).

The big importance of insects and their ecological services to the human kind is a big issue in today’s society. Many people question the use of pesticides and are blaming chemicals and conventional farming for the loss of beneficial insects. With this in mind people instead turn to the organic farming. The aim of this study was to try and find out if organic farming benefits the diversity of insects. The study was performed on a farm in South Africa which practice both conventional and organic farming and also has a natural Fynbos field on its property. The insect diversity was compared in these three different fields. The collecting of insects was performed by using two different methods, using pan traps and pitfall traps. This study shows that there is a significant difference in the amount of species among the different fields, with a significant higher insect diversity in the Fynbos compared to the conventional farming area, and the diversity of insects in the organic farming is somewhere between the natural Fynbos and the conventional farming. In conclusion this study stress that we need more understanding about what critical factors in the organic farming that supports higher diversity and the importance of surrounding farms that also may play a role to increase the biological diversity to cultivated fields.