Background: In healthcare, it is common that personnel handle medical gases. Medical gases can be hazardous if they are handled the wrong way. The healthcare personnel needs knowledge within gas handling to eliminate the risks of mix-ups, inaccurate transportation, inaccurate change of regulators and further on. Therefore, it is essential to educate healthcare personnel in how to handle medical gases in a safe manner. Region Uppsala has been responsible for educations on-site. To make the education more accessible it is desirable to make an interactive online course, with personnel responsible for medical gases as the target group. Aim: To design a digital interactive education, with personnel responsible for medical gases as the target group. Method: Revise material from earlier education plans within gas handling, designing an interactive online course using Articulate 360 storyline and evaluate the pedagogical layout and the information in the first version of the online course. Result: It was found that an interactive online course, with personnel responsible for medical gases within Region Uppsala as the target group should contain general information about gas, medical gases with associated bottles, information about gas cylinder, regulators and their handling and security and management processes. Focus during the making of the online course was to include interactive parts and to follow the principles “Meaningful”, “Engaging” and “Accessible”. The result from the evaluation made on the first version of the education generated about ten points of improvement. Two examples of changes applied to the final version of the education was a change of color from black to white on the icons at the bottom of the layout and addition of a section about emergency shut-off valves. Conclusion: An interactive online course was produced with the purpose to educate personnel responsible for medical gases within Region Uppsala. The created online course is more accessible than the previous education available on-site and with some complementary practical elements, the online course can permanently replace the previous on-site education.