Darbo tikslas buvo įvertinti biokatalizatorių įtaką akrilamido formavimuisi ruginės duonos gaminiuose bei RS 190 modifikuotų mielių įtaką kvietinių miltų 550 C tipo technologinių savybių pagerinimui, pritaikant juos pusruginės duonos gamybai. Nustatyta, kad L. delbruecki intensyviau mažina terpės pH (3,35) nei P. acidilactici (4,32), tačiau, žaliavos apcukrinimui naudojant gliukoamilolitinius fermentus, fermentacijos procesą galima suintensyvinti. Žaliavos apcukrinimas fermentais įtakoja didesnį P. acidilactici proteolitinių fermentų aktyvumą rauguose. L. delbruecki fermentuoti kepiniai gaunami geresnės kokybės nei fermentuoti P. acidilactici. P. acidilactici fermentuotų kepinių kokybę galima pagerinti naudojant optimalų gliukoamilazės kiekį (0,05 ml). 0,030 ml gliukoamilazės panaudojimas žaliavos apcukrinimui, lėtina kepinių žiedėjimą (minkštimo struktūra pakito: su 0,03 ml – 2,15, su 0,05 ml – 2,54 karto). Akrilamido formavimuisi turi įtakos ne tik fermentacijai naudotos PRB, bet ir kepinio dydis (500 g kepiniuose vidutinis akrilamido kiekis 61,55 µ/kg, 1000 g - 40,48 µ/kg). Tirtų kvietinių miltų fizikinės struktūrinės savybės yra tinkamos mielinių kepinių gamybai, o modifikuotų mielių priedas yra tinkama priemonė kvietinių kepinių kokybės rodikliams pagerinti. 550 C tipo kvietinius miltus su modifikuotų mielių priedu panaudojus pusruginių kepinių gamybai galima pagerinti kepinių akytumą ir padidinti drėgnį, tačiau, kitos savybės nepakinta, arba gaunamos prastesnės. Šis... [toliau žr. visą tekstą] / The goal of the thesis was to assess the impact of biocatalysts on the formation of acrylamide in rye bread and to determine the impact of genetically modified yeast RS 190 on improvement of technological properties of wheat flour 550 C by applying them for semi-rye bread making. It has been established that L. delbruecki decreases pH (3,35) medium more intensively than P. acidilactici (4,32), however by using glucoamylolytic enzymes for scald making fermentation process may be intensified. The use of enzymes for scald production results in a better activity of P. acidilactici proteolytic enzymes in leaven. L. delbruecki fermented bread are received of a better quality than fermented P. acidilactici. The quality of P. acidilactici fermented bread may be improved by using an optimum amount of glucoamylase (0,05 ml). Using of 0,030 ml glucoamylase for the scald producion slows staling process of bread (structure of the crumb changed as follows: with 0,03 ml – 2,15, with 0,05 ml – 2,54 times). The formation of acrylamide is influenced not only by lactic acid bacteria (LAB) used for fermentation, but also, by the size of bread (in 500 g pastry goods – midle acrylamide content61,55 µ/kg, in 1000 g - 40,48 µ/kg) as well. Physical structural properties of investigated wheat flour are suitable for making bread, whereas genetically modified yeast supplement is a suitable means for improvement of quality indicators of wheat bread. By using 550 C type wheat flour with... [to full text]

This thesis looks at the divergent policy decisions on the commercial release of two genetically modified (GM) crops in India. Bt cotton was introduced in India in 2002, and has spread widely across the country, though not without controversy. In 2010, the first GM food crop – Bt Brinjal (eggplant) – was put forward for approval. In contrast to the Bt cotton decision, and following heated debate and a series of public consultations across the country, an indefinite moratorium was placed on the crop. In this thesis, I unpack the various factors that shaped both decisions and the politics that accompanied them. To facilitate this analysis, I use a conceptual framework that combines four key forces that are often the focus of food governance analysis, but which are not always considered together. I argue that the divergence in outcomes can be explained by a confluence of shifts in key elements of the policy process. These dimensions of influence can be understood in four related categories: corporate actors, institutional mechanisms, science and science networks, and discursive elements. Changes that took place in each of these dimensions in the period between the two decisions align to explain divergent outcomes that no individual influence could. The conceptual framework I develop in this thesis presents a useful structure to analyse the often-complex and multi-causal processes and outcomes related to food and environmental issues. The results of this research have implications for the future direction of agricultural GMO policy in India, as well as in other countries in the global South.

Tomato, which is one of the most important component of human diet, has been genetically modified to develop some properties like delayed ripening and insect resistance. In order to give a choice to the consumer, it is necessary to detect and label GM foods. This study was carried out to detect genetically modified tomato samples purchased from different food markets of Turkey. PCR method was used to detect genetically modified insect resistant tomatoes. The DNAs of collected samples were isolated according to CTAB DNA extraction protocol and the amplification capacity of isolated samples were checked with patatin specific control PCR. Screening tests of tomatoes were done by targeting 35S promoter, Nos terminator and NptII kanamycin resistance gene with four primer sets. It was aimed to detect Cry1A and Cry1Ac genes with three PCR systems, in order to identify insect resistant samples. From twenty-eight samples, twenty-two gave positive amplification signal in NptII specific PCR system and results were confirmed with sequence analysis. Additionally, we observed seventeen and ten DNA fragments with Cry1A-F/Cry1A-R and Cry1Ac-F/Cry1Ac-R primer sets respectively, it is necessary to confirm these results with DNA sequencing.

This thesis looks at the divergent policy decisions on the commercial release of two genetically modified (GM) crops in India. Bt cotton was introduced in India in 2002, and has spread widely across the country, though not without controversy. In 2010, the first GM food crop – Bt Brinjal (eggplant) – was put forward for approval. In contrast to the Bt cotton decision, and following heated debate and a series of public consultations across the country, an indefinite moratorium was placed on the crop. In this thesis, I unpack the various factors that shaped both decisions and the politics that accompanied them. To facilitate this analysis, I use a conceptual framework that combines four key forces that are often the focus of food governance analysis, but which are not always considered together. I argue that the divergence in outcomes can be explained by a confluence of shifts in key elements of the policy process. These dimensions of influence can be understood in four related categories: corporate actors, institutional mechanisms, science and science networks, and discursive elements. Changes that took place in each of these dimensions in the period between the two decisions align to explain divergent outcomes that no individual influence could. The conceptual framework I develop in this thesis presents a useful structure to analyse the often-complex and multi-causal processes and outcomes related to food and environmental issues. The results of this research have implications for the future direction of agricultural GMO policy in India, as well as in other countries in the global South.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv. / Härtill 6 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst., 2003. / Härtill 5 uppsatser.

Thesis (M.Sc.) - Carleton University, 2006. / Includes bibliographical references (p. 137-151). Also available in electronic format on the Internet.

Thesis (Ph. D.)--University of Nevada, Reno, 1998. / Includes bibliographical references. Online version available on the World Wide Web.

Thesis (Ph.D.) -- University of Texas Southwestern Medical Center at Dallas, 2005. / Not embargoed. Vita. References located at the end of each chapter.

Thesis (Ph. D.)--Ohio State University, 2006. / Title from first page of PDF file. Includes bibliographical references (p. 115-119).