Le défi de la protection des espèces menacées en Afrique est de concilier de façon durable les objectifs de conservation de la nature, et la nécessité de développement économique des populations humaines. Au Niger, survivent aujourd'hui les dernières girafes d'Afrique de l'Ouest (Giraffa camelopardalis peralta). Ces girafes sont unique à plusieurs titres : (i) elles constituent les seules représentantes de la sous espèce peralta (ii), elles vivent dans une région fortement anthropisée (iii) elles vivent dans une zone non protégée, (iv) et elles ne cohabitent avec aucun prédateur. En 1996, la population était au bord de l'extinction avec seulement 50 individus. Malgré des signes de restauration observés ces dernières années, la sous-espèce est inscrite depuis 2008 dans la liste rouge des espèces menacées de l'UICN (Union Internationale pour la Conservation de la Nature) avec le statut « en danger ». Quels sont les facteurs qui pourraient limiter le taux de croissance maximum récemment observé dans cette population? Est-ce que les conjonctures favorables à l'augmentation de la population sont durables? Aborder les questions de conservation des populations met en avant le besoin de connaitre les paramètres démographiques de la population concernée, et les paramètres environnementaux qui conditionnent sa distribution spatiale. Nous avons réalisé une analyse de l'historique des comptages, puis, nous avons déterminé les paramètres démographiques de la population grâce à une approche par Capture Marquage Recapture. Une analyse spatiale à plusieurs échelles nous a permis de caractériser la distribution des girafes aux niveaux de la population et du troupeau (à partir d'observations directes), et de mesurer la sélection de l'habitat au niveau individuel (à partir d'un suivi par colliers GPS) / One of the main challenges for endangered species protection in Africa is to find a sustainable way of integrating objectives of nature conservation with the economic development needs of the local human population. Last West African giraffe population, Giraffa camelopardalis peralta, lives in Niger. These giraffe are unique for several reasons: (i) they represent the only population of peralta sub-species, and (ii) they live in an area densely populated by humans, (iii) which is unprotected and (iv) without predators. In 1996, this giraffe population was almost extinct, with only 50 individuals remaining. Despite signs of population recovery, the sub-species has been classified as “endangered” according to the IUCN Red List assessment criteria. What are the limiting factors for the maximum annual growth rate that has been recorded over the last years in the population? Are favorable conjunctures to this population increasing sustainable? Assessing population conservation requires knowledge of demographic parameters and understanding of the environmental factors driving its spatial distribution. Census data from 1996 to 2009 were analyzed and then demographic parameters through a capture-markrecapture method were determined. A multi-scale spatial analysis allowed me to determine giraffe distribution at both population and herd level (through observations), and to measure habitat selection at the individual level (through GPS satellite collars). Census results, almost exhaustive from 2005 to 2008 highlited an annual growth rate of 12%. This is the maximum growth rate for a giraffe population, and fits with the theoretical maximum growth rate for the species. At the population and herd level, giraffe distribution patterns are driven by food availability. These food resources are seasonally distributed and impacted by human activities. Habitat selection shows that during dry season, giraffe avoid village proximity, where disturbance is high. However, at night giraffe move closer to villages where food resource quantity and quality are higher (tree density, granaries). The use of bean field crop suggests that some cultivated crops gain in attraction and even become favourable to giraffe. This might explain the increase of human-giraffe conflicts. My results clearly show the importance of taking human activities and perception into account, when assessing wildlife conservation strategies

Da die afrikanischen Spitzmaulnashörner in ihrer Heimat vom Aussterben bedroht sind, besitzt ihre Nachzucht in Zoologischen Gärten große Bedeutung. Zwar sind die Bestände der Giraffen in der Wildnis noch nicht besonders gefährdet, die Verlustrate dieser empfindlichen Tierart in menschlicher Obhut ist jedoch zu hoch. Die Gestagen-konzentrationen im Kot wurden bestimmt, um detailliertere Kenntnisse der Fortpflan-zungsphysiologie dieser beiden Spezies, die für eine erfolgreiche Reproduktion notwendig sind, zu erlangen. Die nichtinvasive Methode erwies sich als geeignet zur Überwachung der Fortpflanzung sowohl bei den Giraffen als auch den Spitzmaulnas-hörnern. Die Gestagenbestimmung im Kot erfolgte in 3 Schritten: Einwiegen des Kotes, Extraktion der Gestagene mit Hilfe von Methanol und ihre Bestimmung mittels Radioimmunoassay. Im methodischen Teil der Arbeit ergaben sich innerhalb einer Tierart (Giraffen, Spitzmaulnashorn und zusätzlich Damagazellen) nur geringfügige Schwankungen der Trockenmasse des Kotes von maximal 5 %, so dass sich auch bei unterschiedlichem Wassergehalt des Kotes ohne vorherige Trocknung der Proben gut vergleichbare Gestagenwerte in verschiedenen Kotproben der gleichen Tierart ermitteln lassen. Nach 24- bzw. 48-stündiger Lagerung bei Raumtemperatur waren im Kot von Giraffen und Nashörnern die Gestagenwerte im Vergleich zum sofortigen Einfrieren der Proben signifikant erhöht. Bei Nashörnern und Gazellen wurden nach längerer Lagerzeit (1 und 3 Monate) bei – 20 °C keine signifikanten Veränderungen der niedrigen Gestagen-konzentrationen im Kot festgestellt. Im Gegensatz dazu zeigte sich bei Kotproben von Giraffen mit hohen Ausgangskonzentrationen eine signifikante Erniedrigung (durchschnittlich 45 %). Im Vergleich zu einmaligem führte mehrmaliges Auftauen der Proben zum signifikanten Absinken der Gestagenkonzentrationen im Kot von Spitzmaulnashörnern und Gazellen. Im Ergebnis dieser Voruntersuchungen wurde für das weitere Vorgehen eine standardisierte Behandlung der Kotproben bis zur Bestimmung ihrer Gestagenkonzentrationen eingehalten. Bei 13 Giraffen und 8 östlichen Spitzmaulnashörnern aus 7 deutschen Zoos (insgesamt 2618 Kotproben) erfolgte zwischen 1997 und 2002 eine Zyklus- und/ oder Graviditäts-diagnostik mittels Gestagenbestimmungen im Kot. Dabei zeigten die Konzentrationen an Progesteronmetaboliten im Kot von 6 adulten, ingraviden Giraffen zyklische Schwankungen mit einer Zykluslänge von ca. 14 Tagen. Die Follikelphase dauerte im Mittel 6,9 Tage mit Gestagengehalten von durchschnittlich 259 ± 49 ng/g Kot und die Lutealphase hatte eine Länge von im Mittel 7,6 Tagen bei Konzentrationen an Progesteronmetaboliten von durchschnittlich 1163 ± 223 ng/g Kot. Brunstsymptome und/ oder Paarungen fielen immer mit dem Ende der Lutealphase zusammen. Am Beginn von 8 Graviditäten kam es bei den Giraffen zum Anstieg der Hormonkonzen-trationen auf Werte, die auch während der Lutealphase erreicht werden. Danach blieb die Gestagenausscheidung mit dem Kot zwischen der 58. und 1. Woche a. p. auf hohem Niveau. Eine Rückkehr auf Basalwerte, die während der Follikelphase auftreten, erfolgte erst 3 Tage p. p. Nach der Geburt konnten bei einigen Tieren postpartale Östren mit einer kurzfristigen Erhöhung der Hormonausscheidung im Kot festgestellt werden. Bei 7 adulten, ingraviden Spitzmaulnashörnern konnte mit der angewandten Methode kein Sexualzyklus ermittelt werden. Diese Tiere zeigten nur geringe Schwankungen der Gestagenausscheidung auf niedrigem Niveau (im Mittel 74 ± 18 ng/g Kot). Im Rahmen von 4 Graviditäten kam es bei den Spitzmaulnashörnern zunächst zu einer langsamen Erhöhung der Ausscheidung von Progesteronmetaboliten mit dem Kot, gefolgt von einem starken Anstieg ab der 56. Woche a. p. auf maximale Konzentrationen von ca. 674 ng/g Kot zwischen der 40. und 36. Woche a. p. Im weiteren Verlauf der Gravidität schwankten die Gestagengehalte zwischen 450-600 ng/g Kot. Eine Rückkehr auf das Niveau der Gestagenausscheidung von ingraviden Tieren war erst 3 Tage p. p. zu verzeichnen. Durch Festlegung eines Schwellenwertes von 200 ng/g Kot konnte eine Graviditätsdiagnose bei den Spitzmaulnashörnern ab etwa 52 Wochen vor der Geburt erfolgen. Eine Vorhersage des Geburtszeitpunktes war durch die Bestimmung der Gestagene im Kot weder bei Giraffen noch bei Spitzmaulnashörnern möglich. / Since the African black rhinoceros is threatened to become extinct in its homeland, its offspring in zoological gardens possesses great importance. The existence of the giraffe is not yet particularly endangered in the wild, the loss of this sensible species in captivity is however very high. Gestagen concentrations in the faeces were determined in order to get more knowledge on the reproduction physiology of these two species, which is necessary for a successful reproduction. These non-invasive method was shown to be suitable for monitoring of the reproduction both in giraffes and black rhinoceroses. The gestagens in the faeces were analyzed in 3 steps: weighing of faeces specimens, gestagen extraction with methanol and their determination by means of radioimmunoassay. In the methodical part of the study the dry mass of the faeces showed only small variations up to 5 % within one species (Baringo giraffe, black rhinoceros and also dama gazelle). Thus, it was possible to estimate comparable gestagen levels from several faecal samples within one species without drying, in spite of their different amounts of water. After storage at room temperature for about 24 and/ or 48 hours gestagen concentrations in the faeces of giraffes and rhinoceroses were significantly increased in comparison to samples frozen immediately. After prolonged storage time (1 and 3 months) at – 20 °C no significant changes of low gestagen concentrations were stated in the faeces of rhinoceroses and gazelles. In opposite to this, in the faeces of giraffes with high initial gestagen concentrations a significant decrease (average 45 %) was evident. Repeated thawings of the samples led to a significant dropping of the gestagen levels in the faeces of rhinoceroses and gazelles compared to single thawing. As a result of these preceding investigations a standardized treatment of the faeces samples prior to determination of their gestagen concentrations was observed. Control of reproduction cycle and pregnancy respectively by means of faecal gestagen monitoring was carried out in a total of 2618 faecal samples of 13 giraffes and 8 eastern black rhinoceroses, collected in 7 German zoos from 1997 to 2002. Concentrations of progesterone metabolites in the faeces of 6 adult, nonpregnant giraffes showed cyclic fluctuations with a cycle length of approximately 14 days. The follicular phase took 6.9 days on an average with a mean gestagen concentration of 259 ± 49 ng/g faeces and the luteal phase had a length of 7.6 days on an average with a mean concentration of 1163 ± 223 ng/g faeces. Oestrus behaviour and/ or mating was observed always at the end of the luteal phase. A rise of hormone concentrations to a level, which is characteristic for the luteal phase, was evident at the beginning of 8 pregnancies in giraffes. Afterwards the excretion of faecal gestagens remained on a high level between week 58th and 1st a. p. Basal values, which are characteristic for the follicular phase, were detected 3 days p. p. After parturition some animals showed oestrus behaviour with a short increase of hormone excretion by the faeces. In 7 adult, nonpregnant black rhinoceroses no reproduction cycle could be ascertained by determination of gestagens in the faeces. Only small fluctuations of the gestagen excretion on a low level (on an average 74 ± 18 ng/g faeces) were evident in these animals. Within 4 pregnancies of black rhinoceroses a slow increase of the excretion of faecal progesterone metabolites could be detected, followed by a massive rise from week 56th a. p. to maximum concentrations of approximately 674 ng/g faeces between week 40th and 36th a. p. In the ongoing pregnancy the gestagen concentrations varied between 450-600 ng/g faeces. A return to the level of the gestagen excretion of nonpregnant animals was noticed 3 days p. p. Diagnosis of pregnancy of black rhinoceroses was possible approximately 52 weeks prior to parturition by defining a threshold value of 200 ng/g faeces. Prediction of the day of delivery by means of gestagen determination in the faeces was neither possible in giraffes nor in black rhinoceroses.

One of the principles regarding our knowledge about life on earth is that no organism can be fully understood without taking into account its surrounding environment. This study examines the extent to which ecosystem-focused field studies may be associated with students' academic performance and potential to contribute to the advancement of science and conservation. Pilot studies conducted in Panama and California established methods used in this project from 1993-2003. Two hundred and sixty-seven students conducted field studies in either Kenya or Costa Rica. Students worked in cognitive apprenticeships, in which research staff trained each student to conduct field research. At the conclusion of their fieldwork, the research staff assessed the students written field reports. The students' reports were then sorted according to the extent to which their studies were ecosystem-focused. Data analysis through nonparametric, Kruskal-Wallis statistical tests revealed no significant difference in academic performance on field study reports, in regards to whether the studies were narrow in scope (species-specific) or broad (ecosystem-focused). Marginal significance was revealed between ecosystem-focused studies and the potential for students' fieldwork to contribute to the advancement of science and conservation. Also addressed in the data were injuries, disease, and potential hazards, which were influenced by prudent and decisive leadership. Successful field studies require consideration of the content, context, and design of the intended field projects. Many of the solutions to environmental problems on the planet will come from the working hands of teachers and students that conduct explorations in the field. / Life Sciences in Education / D. Phil. (Mathematics, Science and Technology Education)

Plus de 300 planètes extrasolaires ont été découvertes à ce jour. La variété et la diversité des caractéristiques qu'elles présentent sont extrêmement vastes. Et dans cette multitude, une population se distingue, les Jupiter-chauds. Ces planètes ne ressemblent, en effet, en rien à celles que l'on côtoie dans le Système Solaire. Elles ont une masse d'une à plusieurs fois celle de Jupiter et sont très proches de leur étoile parent, orbitant en seulement quelques jours. L'étude de cette population nous apporte beaucoup d'éléments quant à leur processus de formation et d'évolution. Certaines d'entre elles ont en effet la particularité de transiter devant leur étoile parent. Mes travaux de thèse m'ont mené à détecter et caractériser en densité 14 planètes extrasolaires, grâce aux programmes de recherche CoRoT et SuperWASP combinés au spectrographe SOPHIE (OHP). Parmi ces planètes figurent celles ayant la plus courte période orbitale et, autour d'une autre étoile, la plus importante masse jamais détectées en transit. Un programme novateur, que j'ai initié, nous a également permis d'explorer les capacités de détection d'un instrument de type multi-fibre (FLAMES/GIRAFFE et UVES). Nous avons montré que cet instrument peut être performant en terme de précision de mesure en vitesse radiale, et qu'il permet un très bon écrémage des cibles. L'instrument multi-fibre est utilisable également dans le cadre d'un suivi de candidats issus de programmes de recherche par photométrie, tels que la mission CoRoT , mais il nécessite dans ce cas, pour être eficace, d'un champ de vue significatif (de plusieurs degrés carrés par exemple). L'étude du transit spectroscopique de 3 systèmes planétaires m'a également permis d'apporter de fortes contraintes quant à leur processus de formation et d'évolution, et de mettre en évidence pour la première fois l'existence d'un système exotique : X0-3.

One of the principles regarding our knowledge about life on earth is that no organism can be fully understood without taking into account its surrounding environment. This study examines the extent to which ecosystem-focused field studies may be associated with students' academic performance and potential to contribute to the advancement of science and conservation. Pilot studies conducted in Panama and California established methods used in this project from 1993-2003. Two hundred and sixty-seven students conducted field studies in either Kenya or Costa Rica. Students worked in cognitive apprenticeships, in which research staff trained each student to conduct field research. At the conclusion of their fieldwork, the research staff assessed the students written field reports. The students' reports were then sorted according to the extent to which their studies were ecosystem-focused. Data analysis through nonparametric, Kruskal-Wallis statistical tests revealed no significant difference in academic performance on field study reports, in regards to whether the studies were narrow in scope (species-specific) or broad (ecosystem-focused). Marginal significance was revealed between ecosystem-focused studies and the potential for students' fieldwork to contribute to the advancement of science and conservation. Also addressed in the data were injuries, disease, and potential hazards, which were influenced by prudent and decisive leadership. Successful field studies require consideration of the content, context, and design of the intended field projects. Many of the solutions to environmental problems on the planet will come from the working hands of teachers and students that conduct explorations in the field. / Life Sciences in Education / D. Phil. (Mathematics, Science and Technology Education)

Habitat loss and fragmentation is a crisis affecting wildlife worldwide. In Tanzania, East Africa, a dramatic and recent (<80 years) expansion in human settlement and agriculture threatens to reduce gene flow among protected areas for many species of large mammals. Wildlife linkages can mitigate population isolation, but linkage designs lacking empirical justification may be controversial and ineffective. Connectivity conservation requires an understanding of how biogeographic factors shaped gene flow prior to habitat loss or fragmentation, however the history of interaction among populations is rarely known. The goal of my study was to provide context for connectivity conservation in central and southern Tanzania by identifying landscape features that have shaped gene flow for three ungulate species with different dispersal capabilities. I investigated historical patterns of connectivity for Maasai giraffe (Giraffa camelopardalis tippelskirchi), impala (Aepyceros melampus), and eland (Tragelaphus oryx) by estimating genetic structure among four to eight protected areas per species. Genetic structure changes very slowly among large populations and thus is likely to reflect historical processes instead of recent anthropogenic influences. I collected noninvasive DNA samples and generated microsatellite genotypes at 8 to 15 loci per species, then estimated genetic diversity metrics (allelic richness, AR, and expected heterozygosity, H[subscript E]) for each population (defined by reserve). I also calculated genetic distance (F[subscript ST] and Nei's unbiased genetic distance, D[subscript hat]) and an estimate of gene flow (Nm) between all population pairs for each species. To elucidate the possible causes of genetic structure between these populations, I tested for isolation by distance and isolation by resistance based on a suite of biogeographic factors hypothesized to affect gene flow. To do this, I created GIS-based resistance surfaces that assigned different costs of movement to landscape features. I created one or more resistance surfaces for each hypothesis of landscape effect. I used circuit theory to estimate the cumulative resistance between each pair of reserves for each weighting scheme, and then performed Mantel tests to calculate the correlation between these resistances and the observed population pairwise genetic distances (D[subscript hat]). I chose the optimal resistance model for each species as the model that was most highly correlated with observed genetic patterns. To verify that the correlation of resistance models with genetic distance was not an artefact of geographic distance, I performed partial Mantel tests to calculate correlation while controlling for the effect of geographic distance. Finally, I compared historical gene flow patterns to the distribution of contemporary human activity to predict areas that are at risk of a loss of connectivity. Indices of genetic diversity were moderate for all three species and comparable to previously reported values for other savannah ungulates. Diversity (both H[subscript E] and A[subscript R]) was highest in eland and lowest in giraffe for these populations, and was not consistently correlated with reserve size as has been reported for other species in East Africa. Although patterns in genetic distance were broadly similar across all three species there were also striking differences in connectivity, highlighting the importance of cross-species comparisons in connectivity conservation. At this scale, resistance models based on slope strongly predicted population structure for all three species; distance to water was also highly correlated with genetic distance in eland. For all three species, the greatest genetic distances occurred between populations separated by the Eastern Arc Mountains, suggesting that the topography of this area has long acted as a barrier to gene flow, but this effect is present in varying degrees for each species. I observed high levels of historical gene flow between centrally located populations (Ruaha National Park and Rungwa Game Reserve) and those in the southwest (Katavi National Park and Rukwa Game Reserve). Although human settlement in this area has been low relative to other areas, the connection between the Katavi/Rukwa and Ruaha ecosystems may be threatened by increased human activity and warrants conservation. High levels of historical gene flow were also seen between reserves in the northeast (Tarangire National Park, Swagaswaga Game Reserve) and the central and southwest populations. These connections appear highly threatened due to current land use practices, and may have already suffered a loss of gene flow. Field surveys in the lands surrounding the northeastern reserves are needed to quantify current levels of connectivity and determine whether corridors could be established to maintain or restore gene flow with other reserves. / Graduation date: 2012