Cleaved fragments of the extracellular matrix protein perlecan have been shown to promote neuroprotection and repair after ischemic stroke. The cysteine proteases cathepsin B and L as well as the metalloprotease bone morphogenic protein 1 (BMP-1) are capable of releasing the biologically active C-terminal laminin-like globular domain (LG3) of perlecan. Exercise, a known method of reducing stroke risk and severity, has been shown to increase the expression of some proteases associated with perlecan processing. Using a transient distal middle cerebral artery occlusion (MCAo) model for focal ischemic stroke we show that while 7 days of running only slightly decreased infarct volume, BMP1 and perlecan (HSPG2) RNA expression in skeletal muscle was significantly increased in 3-month-old male wild type C57/BL6 mice. Moreover, elevated levels of BMP1 RNA were still detectable after 3 days of detraining, suggesting a prolonged effect of exercise on BMP1 expression. Levels of LG3 in the blood were below the limit of detection in the current study, however it is likely that a more sensitive method would enable analysis of serum. These preliminary findings suggest that LG3 could be a molecular mediator of neuroprotection afforded by exercise though further studies are required.

Cette thèse utilise le relevé de donné du Panoramic Survey Telescope and Rapid Response System 1 Survey pour trouver de nouveaux satellites du Groupe Local: les galaxies naines et les amas globulaires. Le relevé est important pour résoudre les tensions entre les observations et les modèles. Premièrement, un algorithme de détection est développé, découvrant cinq nouveaux satellites. Bien que cinq découvertes soient faites, le nombre de découvertes est inférieur à ce qu’on s’attendrait, en présumant une distribution isotrope de galaxies naines. Ce résultat mène au deuxième objectif de la these: quantifier les limites de détections du relevé PS1. Les cartes d’efficacité de détection du ciel complet peuvent être utilisées pour quantifier la distribution (an)isotrope des galaxies satellites de la Voie Lactée. En outre, ces informations peuvent mener a redériver la fonction de luminosité des satellites. / This thesis uses the Panoramic Survey Telescope and Rapid Response System 1 Survey to find new Local Group satellites such as dwarf galaxies and globular clusters. This survey is instrumental in helping resolve tensions that have become apparent between observation and theories. In a first phase, a search algorithm is developed, discovering five new satellites. Though yielding five discoveries, this number is lower than one would expect, assuming isotropy of the dwarf galaxies. This leads to the second aim of this thesis, namely quantifying the detection limits of the PS1 Survey. The detection efficiency maps over the entire PS1 sky can be used as a stepping–stone towards the quantification of the (an)isotropy of the Milky Way satellites’ distribution. Using this information, the luminosity function of these satellites can be re–derived.

L’étude des amas globulaires (AGs) nous offre une opportunité d’appréhender l’histoire de leurs galaxies hôtes et ainsi l’histoire de l’univers. Dans cette thèse, je me suis intéressé aux propriétés des AGs dans différents environnements. Tout d’abord, je me suis concentré sur les AGs de l’amas de la Vierge, un amas de galaxies très dense situé à environ 16,5 Mpc. J’ai utilisé les données observées par le relevé NGVS (Next Generation Virgo Survey) pour définir un échantillon qui contient 1846AGs. J’ai ensuite comparé les couleurs de ces amas avec celles d’autres AGs originaires de la Voie Lactée et j’ai remarqué des différences de couleurs encore jamais observées, dont la nature exacte est encore énigmatique. Pour comprendre ces différences, j’ai ensuite comparé les AGs observés avec des AGs synthétiques basés sur 10 modèles de synthèse de populations stellaires. J’ai aussi étudié les âges et les métallicités ressortant de la confrontation directe des couleurs des AGs à ces modèles. En conclusion, en l’état actuel, les modèles ne rendent pas compte de la diversité identifiée dans ma thèse. Finalement, j’ai effectué une brève étude des propriétés spatiales des AGs autour de M87 pour repérer des marques d’accrétion. / Through the study of the globular cluster (GC) properties, it is possible to unravel the history of their host galaxies and by extension the history of the universe. During this thesis, I was interested in the GC properties in different environments. First, I looked at the GCs in the Virgo cluster, a dense galaxy cluster located at 16.5 Mpc. I used data from the survey NGVS (Next Generation Virgo Survey) to define a sample of 1846 GCs. Then, I compared the colors of these GCs with those of Milky Way GCs and I noted color differences never yet observed, which are still enigmatic. In order to understand these differences, I compared the observed GCs with synthetic GCs obtained with 10 stellar population synthesis models. I also studied the age and metallicity predictions of those models. In the end, in their current status, the models do not account for the diversity highlighted in my thesis. Finally, I assessed the spatial properties of the GCs around M87 in order to find any signatures of a recent accretion.

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No description available.

<p>In this thesis, we present theoretical and statistical techniques broadly related to systems of dynamically-interacting particles. We apply these techniques to observations of dense star clusters in order to study gravitational interactions between stars. These include both long- and short-range interactions, as well as encounters leading to direct collisions and mergers. The latter have long been suspected to be an important formation channel for several curious types of stars whose origins are unknown. The former drive the structural evolution of star clusters and, by leading to their eventual dissolution and the subsequent dispersal of their stars throughout the Milky Way Galaxy, have played an important role in shaping its history. Within the last few decades, theoretical work has painted a comprehensive picture for the evolution of star clusters. And yet, we are still lacking direct observational confirmation that many of the processes thought to be driving this evolution are actually occuring. The results presented in this thesis have connected several of these processes to real observations of star clusters, in many cases for the first time. This has allowed us to directly link the observed properties of several stellar populations to the physical processes responsible for their origins.</p> <p>We present a new method of quantifying the frequency of encounters involving single, binary and triple stars using an adaptation of the classical mean free path approximation. With this technique, we have shown that dynamical encounters involving triple stars occur commonly in star clusters, and that they are likely to be an important dynamical channel for stellar mergers to occur. This is a new result that has important implications for the origins of several peculiar types of stars (and binary stars), in particular blue stragglers. We further present several new statistical techniques that are broadly applicable to systems of dynamically-interacting particles composed of several different types of populations. These are applied to observations of star clusters in order to obtain quantitative constraints for the degree to which dynamical interactions affect the relative sizes and spatial distributions of their different stellar populations. To this end, we perform an extensive analysis of a large sample of colour-magnitude diagrams taken from the ACS Survey for Globular Clusters. The results of this analysis can be summarized as follows: (1) We have compiled a homogeneous catalogue of stellar populations, including main-sequence, main-sequence turn-off, red giant branch, horizontal branch and blue straggler stars. (2) With this catalogue, we have quantified the effects of the cluster dynamics in determining the relative sizes and spatial distributions of these stellar populations. (3) These results are particularly interesting for blue stragglers since they provide compelling evidence that they are descended from binary stars. (4) Our analysis of the main-sequence populations is consistent with a remarkably universal initial stellar mass function in old massive star clusters in the Milky Way. This is a new result with important implications for our understanding of star formation in the early Universe and, more generally, the history of our Galaxy. Finally, we describe how the techniques presented in this thesis are ideally suited for application to a number of other outstanding puzzles of modern astrophysics, including chemical reactions in the interstellar medium and mergers between galaxies in galaxy clusters and groups.</p> / Doctor of Philosophy (PhD)

Mergers are known to be essential in the formation of large scale structures and to have a significant role in the history of galaxy formation and evolution. Besides a morphological transformation, mergers induce important bursts of star formation. These starburst are characterised by high Star Formation Efficiencies (SFEs) and Specific Star Formation Rates, i.e., high Star Formation Rates (SFR) per unit of gas mass and high SFR per unit of stellar mass, respectively, compared to spiral galaxies. At all redshifts, starburst galaxies are outliers of the sequence of star-forming galaxies defined by spiral galaxies. We have investigated the origin of the starburst-mode of star formation, in three local interacting systems: Arp 245, Arp 105 and NGC7252. We combined high-resolution JVLA observations of the 21-cm line, tracing the Hi diffuse gas, with UV GALEX observations, tracing the young star-forming regions. We probe the local physical conditions of the Inter- Stellar Medium (ISM) for independent star-forming regions and explore the atomic-to-dense gas transformation in different environments. The SFR/H i ratio is found to be much higher in central regions, compared to outer regions, showing a higher dense gas fraction (or lower Hi gas fraction) in these regions. In the outer regions of the systems, i.e., the tidal tails, where the gas phase is mostly atomic, we find SFR/H i ratios higher than in standard Hi-dominated environments, i.e., outer discs of spiral galaxies and dwarf galaxies. Thus, our analysis reveals that the outer regions of mergers are characterised by high SFEs, compared to the standard mode of star formation. The observation of high dense gas fractions in interacting systems is consistent with the predictions of numerical simulations; it results from the increase of the gas turbulence during a merger. The merger is likely to affect the star-forming properties of the system at all spatial scales, from large scales, with a globally enhanced turbulence, to small scales, with possible modifications of the initial mass function. From a high-resolution numerical simulation of the major merger of two spiral galaxies, we analyse the effects of the galaxy interaction on the star forming properties of the ISM at the scale of star clusters. The increase of the gas turbulence is likely able to explain the formation of Super Star Clusters in the system. Our investigation of the SFR–H i relation in galaxy mergers will be complemented by highresolution Hi data for additional systems, and pushed to yet smaller spatial scales.

Cette thèse présente le travail réalisé par l'auteur consacréà l'étude de l'influence des vents magnétosphériques des pulsars sur leur environnement. Le problème du vent magnétosphérique des pulsars est étudié dans le contexte des pulsars classiques, qui forment des nébuleuses de pulsar. L'observation de ces nébuleuses fournit des informations importantes sur leurs conditions physiques et dynamiques. Le vent magnétosphérique des pulsars milliseconde est également étudié. La contribution des pulsars millisecondeà l'émission gamma des amas globulaires est étudiée par des simulations numériques.Dans la première partie, les résultats des observations infrarouge du vestige de supernova G21.5-0.9 sont présentées. Les données utilisées comprennent des observations du Very Large Telescope de l'ESO, du télescope Canada-France-Hawaï (CFHT) et du télescope spatial Spitzer. La détection de la nébuleuse compacte autour du pulsar PSR J1833-1034, avec l'instrument CFHT/AOB-KIR (bande K') et la caméra IRAC/Spitzer (toutes les bandes), est présentée. La valeur moyenne de la fraction de polarisation linéaire de l'émission détectée est estiméeà $P_{rm L}^{avg} simeq 0.47$. Une oscillation du vecteur champ électrique dans la nébuleuse compacte peut être observée. Le spectre infrarouge de la nébuleuse compacte est bien décrit par une loi de puissance d'indice $alpha_{rm IR} = 0.7 pm 0.3$, et suggère un aplatissement spectral entre les domaines infrarouge et X. La détection de la raie d'émission [Fe II] à 1.64 $mu$m est présentée. La spectroscopieà moyenne résolution permet d'estimer l'extinction par le milieu interstellaire de l'émission infrarouge de l'objet, ainsi que la vitesse d'expansion de la matière émettant la raie du [Fe II], ce qui conduità une estimation de la distanceà G21.5-0.9 de $3.9 pm 1.2$ kpc.La deuxième partie présente une étude de l'activité magnétosphérique des pulsars milliseconde dans le contexte de l'émission gamma des amas globulaires. Une base de données des caractéristiques d'émission des pulsars milliseconde et des spectres d'éjection des électrons est créée sur la base du modèle pair starved polar cap de la magnétosphère des pulsars. Le concept de facteur de biais est introduit et étudié. Des spectres synthétiques d'amas globulaires sont simulés dans la gamme d'énergie allant du GeV au TeV. Ils consistent en une composante d'émission magnétosphérique des pulsars milliseconde résidant dans l'amas, et une composante Compton inverse résultant de la diffusion des photons ambiants (le champ stellaire des photons provenant des étoiles de l'amas, et le fond diffus cosmologique) par les leptons relativistes diffusant dans l'amas. Enfin, les spectres synthétiques des amas globulaires sont comparés et contrastés avec les observationsà haute et très haute énergie des amas globulaires sélectionnés: Terzan 5 et 47 Tucanae. / This PhD thesis presents the results of the studies on the influence of magnetospheric pulsar winds on the surroundings of these objects. The problem of the magnetospheric pulsar wind is studied in the context of classical pulsars, which power prominent pulsar wind nebulae. Observations of these nebulae yield important information on their physical and dynamical conditions. The magnetospheric winds of millisecond pulsars are also investigated. The contribution of millisecond pulsars to the gamma-ray emission of globular clusters is studied through numerical simulations. In the first part of the thesis, the results of infrared observations of the supernova remnant G21.5-0.9 are presented. The observational material includes data obtained with the ESO Very Large Telescope, the Canada-France-Hawaii Telescope and the Spitzer Space telescope. The detection of the compact nebula around the pulsar PSR J1833-1034, through imaging with both the CFHT/AOB-KIR instrument (K' band) and the IRAC/Spitzer camera (all bands), is reported. The average value of the linear polarisation fraction $P_{rm L}^{avg} simeq 0.47$ of the detected emission is estimated. A swing of the electric field vector across the compact nebula is observed. The infrared spectrum of the compact nebula is best described as a power law of index $alpha_{rm IR} = 0.7 pm 0.3$, and suggests its flattening between the infrared and X-ray bands. The detection of [Fe II] 1.64 $mu$m line emitting material is reported. Through medium resolution spectroscopy the infrared interstellar extinction to the object is estimated, and also the expansion velocity of the iron-line emitting material is determined, which in turn leads to estimating the distance of $d = 3.9 pm 1.2$ kpc to G21.5-0.9. The second part presents a study of the magnetospheric activity of millisecond pulsars in the context of the gamma-ray emission of globular clusters. Based on the pair starved polar cap model of the pulsar magnetosphere the database of the millisecond pulsar emission characteristics and the electron ejection spectra is created. The modelled electron ejection spectra are single-peaked for mildly inclined and fast rotating pulsars, while double-peaked for slowly rotating pulsars. The concept of the bias factor is introduced and studied. Synthetic spectra of globular clusters, stretching from MeV up to TeV energies, are simulated. They consist of the magnetospheric contribution from the millisecond pulsar population residing in the cluster, and an inverse Compton scattering (ICS) component resulting from up-scattering of the ambient photon fields (cosmic microwave background and optical photons from stellar population in the cluster) on the relativistic electrons diffusing through the cluster. The spectral characteristics of the ICS component depends on the composition of the ambient photon fields and also on the magnitude of the cluster magnetic field $B_{rm GC}$. For low $B_{rm GC} sim 1 mu$G the spectra are double-peaked. For the high $B_{rm GC} gtrsim 10 mu$G the ICS spectra are single-peaked. The level of ICS emission increases with the increase of $B_{rm GC}$, but it saturates for $B_{rm GC} sim 10 ~mu$G. Finally, the simulated synthetic spectra of globular clusters are confronted with the existing gamma-ray data for selected clusters: Terzan 5 and 47 Tucanae.

Os aglomerados globulares (GCs) são laboratórios essenciais no estudo da formação e evolução quimiodinâmica da Via Láctea, pois formaram-se durante os episódios iniciais de formação estelar das galáxias. Na última década, evidências observacionais fotométricas e espectroscópicas têm desafiado o paradigma clássico da formação dos GCs em um episódio único de formação estelar, dando origem ao debate sobre as múltiplas populações estelares. Com o objetivo de explorar esse fenômeno, o programa do Telescópio Espacial Hubble GO-13297 (UV Legacy Survey of Galactic Globular Clusters, PI G. Piotto) obteve fotometria para 57 GCs com os filtros UV/azul F275W, F336W e F438W (WFC3/UVIS), sensíveis às variações nas abundâncias de CNO e, portanto, capazes de distinguir múltiplas populações ao longo dos diagramas cor-magnitude. Combinados com fotometria anterior nos filtros do óptico F606W e F814W (programa GO-10775, PI A. Sarajedini), eles formam uma base de dados fotométricos sem precedentes para GCs. Este trabalho expõe a análise de sete desses aglomerados, sendo seis do Bojo Galáctico (NGC 6304, NGC 6624, NGC 6637, NGC 6652, NGC 6717 e NGC 6723) e um localizado no Halo interno para referência (NGC 6362). A amostra é representativa dos dois picos na distribuição de metalicidades dos GCs do Bojo, em [Fe/H] ~ -0.5 e -1.0. Adotou-se métodos homogêneos para a separação das múltiplas populações nos diferentes estágios evolutivos e para o ajuste de isócronas, com o intuito de analisar comparativamente os dois grupos de metalicidade e verificar se ocorre alguma diferença de idades detectável entre as múltiplas populações. Algoritmos de classificação com aprendizado de máquina, e métodos utilizando estatística Bayesiana (máxima verossimilhança e MCMC) foram implementados e uma ferramenta em Python, nomeada SIRIUS, foi desenvolvida pelo grupo. Os resultados apontam para uma tendência de idades maiores (~ 13 Gyr) para os GCs mais pobres em metais com ramo horizontal azul, comparado com 12.0-12.5 Gyr para os outros GCs. Os resultados para as múltiplas populações indicam idades ligeiramente mais altas para a primeira geração em geral, mas com diferenças menores que 400 Myr comparado com a segunda geração. O resultado de 13.14 +0.25/-0.43 Gyr para o aglomerado NGC 6717 surpreende pois este é o aglomerado menos massivo da amostra, com maior fração de estrelas da primeira geração e com um ramo horizontal azul estendido: aspectos que o colocam como um dos objetos mais velhos da Galáxia. Esses resultados são bastante relevantes, com impactos diretos nos cenários de formação das partes centrais da Galáxia. / The globular clusters (GCs) are fundamental laboratories to study the formation and chemodynamical evolution of the Milky Way, as they formed during the initial episodes of star formation in galaxies. In the last decade, photometric and spectroscopic observational results have challenged the classical paradigm of GCs formation in a single star formation burst, giving rise to the debate about multiple stellar populations. With the purpose of better explore this phenomenon, the Hubble Space Telescope GO-13297 program (UV Legacy Survey of Galactic Globular Clusters, PI: G. Piotto) obtained photometry for 57 GCs with the UV/blue filters F275W, F336W and F438W (WFC3/UVIS), sensitive to variations in CNO abundances, and consequently able to distinguish multiple populations along the color-magnitude diagrams. Combined with previous photometry in the optical filters F606W and F814W (GO-10775 program, PI A. Sarajedini), they provide an unprecedented photometric database for GCs. This work presents the analysis of seven of these clusters, six of them from the Galactic Bulge (NGC 6304, NGC 6624, NGC 6637, NGC 6652, NGC 6717 and NGC 6723) and one located in the inner Halo for reference purposes (NGC 6362). This sample is representative of the two peaks in the metallicity distribution of Bulge GCs, with [Fe/H] ~ -0.5 and -1.0. We adopted homogeneous methods to the separation of multiple populations in the different evolutionary stages and to the isochrone fitting, with the purpose of analyze comparatively the two metallicity groups, and check whether there occurs any detectable age difference between the multiple populations. Classification algorithms with machine learning, and methods using Bayesian statistics (maximum likelihood and MCMC) were implemented and a Python tool, named SIRIUS, was developed by the group. The results point to a trend of larger ages (~ 13 Gyr) for the more metal-poor clusters with a blue horizontal branch, compared with 12.0-12.5 Gyr for the other GCs. The results for the multiple populations indicate slightly larger ages for the first generation in general, but with differences lower than 400 Myr compared with the second generation. The result of 13.14 +0.25/-0.43 Gyr for the cluster NGC 6717 is surprising because this is the least massive cluster of the sample, with the highest fraction of first generation stars and with a blue horizontal branch: features that place it as one of the oldest objects in the Galaxy. These results are quite relevant, with direct impacts on the formation scenarios of the innermost regions of the Galaxy.

<p>In this thesis, the structure and stability of globular proteins adsorbed onto nanometer-sized hydrophilic silica particles were investigated using differential scanning calorimetry (DSC), hydrogen/deuterium exchange (HDX), and mass spectrometry (MS). The adsorption process itself was characterized with fluorescence and absorption spectroscopy and surface plasmon resonance (SPR). The combination of these methods offered a unique insight into adsorption-induced changes within proteins related to their adsorption characteristics. DSC contributed with thermodynamic information on the overall structural stability within the protein population. HDX in combination with MS contributed information on the structure and stability of adsorbed proteins with focus on changes within the secondary structure elements. In order to increase the structural resolution in this part of the investigation, proteolysis was performed prior to the MS analyzing step. Knowledge on the protein adsorption process was utilized in a practical approach called ligand fishing. In this approach, SPR was used to monitor the chip-based affinity purification of a protein with MS used for protein identification.</p><p>Adsorption isotherms revealed that electrostatic interactions play an important role in the adsorption of proteins to hydrophilic surfaces. DSC investigation revealed that the thermal stability of proteins reduces with increasing electrostatic attraction between the protein and the surface and that this effect diminishes at higher surface coverage. The mass-increase due to exchange between protein hydrogen atoms and deuterium atoms in solution was investigated as a function of time. This gave insight into adsorption-induced changes in the structural stability of proteins. By combining DSC and HDX-MS, it was possible to differentiate between adsorption-induced changes in the secondary and tertiary structure. Additionally, if limited proteolysis was performed, the investigations gave insight into the orientation and protein segment specific changes in the stability of proteins adsorbed to silica surfaces. The adsorption of proteins to silica particles also provided the basis for a new experimental design that allows handling of minute amounts of proteins in a ligand fishing application, as used in the field of functional proteomics.</p>