Yes / We report a simple temperature-responsive bioconjugate system comprising superfolder green fluorescent protein (sfGFP) decorated with poly[(oligo ethylene glycol) methyl ether methacrylate] (PEGMA) polymers. We used amber suppression to site-specifically incorporate the non-canonical azide-functional amino acid p-azidophenylalanine (pAzF) into sfGFP at different positions. The azide moiety on modified sfGFP was then coupled using copper-catalyzed “click” chemistry with the alkyne terminus of a PEGMA synthesized by reversible addition–fragmentation chain transfer (RAFT) polymerization. The protein in the resulting bioconjugate was found to remain functionally active (i.e., fluorescent) after conjugation. Turbidity measurements revealed that the point of attachment of the polymer onto the protein scaffold has an impact on the thermoresponsive behavior of the resultant bioconjugate. Furthermore, small-angle X-ray scattering analysis showed the wrapping of the polymer around the protein in a temperature-dependent fashion. Our work demonstrates that standard genetic manipulation combined with an expanded genetic code provides an easy way to construct functional hybrid biomaterials where the location of the conjugation site on the protein plays an important role in determining material properties. We anticipate that our approach could be generalized for the synthesis of complex functional materials with precisely defined domain orientation, connectivity, and composition. / Engineering and Physical Sciences Research Council (EPSRC), University of Warwick, National Science Foundation (U.S.) (NSF), United States. Defense Advanced Research Projects Agency (DARPA), Seventh Framework Programme (European Commission) (FP7), European Research Council (ERC)

This project began as a critique of the current notions of "green" architecture. It grew into the creation of a system for integrating nature with structure, the organic with the constructed. A grand entry for the Highline Park on the lower Westside of Manhattan is used as a means for generating a domain which plants, specifically hanging ivy, could thrive. Simple elements such as columns, cables, stairs and ramps, can become a means for creating immersive living volumes, fostering instances where nature can conquer construction. / Master of Architecture

Due to our love of the waterfront, almost half of the United States population now lives in coastal areas, including shores of estuaries. Unfortunately, this increasing concentration of people upsets the balance of ecosystems. My thesis project, Designing for the Waterfront - An Estuarine Research Reserve on Jones Point is about setting an example, setting a precedent for building on the waterfront. I have attempted to design an environmentally responsive and sensitive research center that will not only monitor and study the Potomac estuary, but will also serve as an example of how one should build on the estuary. / Master of Architecture

Throughout history, utopian ideals have existed promoting nature as a necessary affect for better aesthetic and psychological being. Yet, as human populations climb so do stresses upon the natural environment - therefore, bringing "the city in harmony with nature" becomes more challenging. Fortunately, hope exists through the use of greenroof technology. Greenroofs are a green space created by continuous layers of drainage, protection, growing medium, and plants either onto or integral to a roofing system. This paper explores extensive greenroofs, characterized by low-maintenance and shallow growing medium. Greenroof benefits (ecological, economical, aesthetic, psychological) are classified as: Market and Human. Further exploration of human-driven benefits result in the definitions of active and passive sensation (the division of sensation): Active sensation is the immediate, present, unimagined engagement of a specific sense. Passive sensation is the imagined perception (sensing) of an object or element. As defined, Active Sensations are real and, therefore, have limits/defects/boundaries; yet, Passive Sensations are imagined, and therefore, limitless. As alluded by William James, "The philosophy which is so important in each of us is not a technical matter; it is our more or less dumb sense of what life honestly and deeply means. It is only partly got from books; it is our individual way of just seeing and feeling the total push and pressure of the cosmos." The remainder of the document explores human-driven greenroof design; emphasizing design as a form of inquiry. / Master of Landscape Architecture

The purpose of the present study is to compare the growth-promoting properties of four varieties of the green cowpea: the blackeye, the red and white crowder, the brown crowder and the cream cowpea.

Over the last 20 years, there has occurred an increase in the number, scope, and impact of nanomaterials projects. By leveraging the Surface Plasmon Resonance of metallic nanoparticles for labelling, sensing, and treatment, researchers have demonstrated the versatile utility of these nanomaterials in medicine. The literature provides evidence of use of simple, well-known chemistry for nanomaterials synthesis when the focus is new applications of nanomaterials. A case in point, is the synthesis of metallic nanoparticles, whereby HAuCl4, CuCl2, Cu(acac)2, and AgNO3 are typically employed as nanoparticle precursors. Unfortunately, the use of these precursors limits the number of applications available to these materials - particularly for AuNPs in medicine, where the byproducts of nanoparticle synthesis (most often surface-adsorbed reductants, toxic stabilizers, and growth directors) cause nanoparticles to fail clinical trials. Despite the several thousand publications detailing the advancements in nanoparticle therapeutics, as of 2017, there were only 50 FDA-approved nanoparticle formulations. Less than 10 were based on metallic nanoparticles. This is a problem because many of these nanoparticle therapeutics demonstrate potent cell killing ability and labeling of cells. A solution to this problem may be the use of weakly coordinated, monovalent metal complexes, which require only one electron to reduce them to their metallic state. Further, by designing nanoparticle syntheses around these monovalent complexes, we can employ weaker, environmentally friendly stabilizers. This strategy also forgoes the use of exogenous reducing agents, because the monovalent complexes can be reduced and stabilized by one reagent. Herein we investigate the use of Au(Me2S)Cl, [Cu(MeCN)4]BF4, and AgBF4 with green stabilizers to synthesize a variety of nanomaterials. We find that a range of sizes of spherical particles, as well as a range of sizes of gold triangular prisms can be synthesized by using techniques that follow this strategy.

This doctoral thesis is focused on applying visible light photocatalysis and deep eutectic solvents in organic transformations and combining the two to develop sustainable procedures. Chapter 1 deals with using visible-light photocatalysis to synthesize olefins via decarboxylative alkenylation from readily available carboxylic acids and vinyl sulfones. The developed protocol employs a metal-free, eco-friendly photocatalyst using a vitamin B2-derived species, achieving alkenes in a highly diastereoselective manner. Further transformations of the obtained compound were carried out while also analysing the reaction mechanism through an array of theoretical calculations and experimental procedures. Chapter 2 consists of the elaboration of a sustainable methodology for the aerobic photooxidation of xanthenes, thioxanthene and 9,10-dihydroacridines, employing a recyclable eutectic mixture as solvent and riboflavin tetraacetate as photocatalyst. This methodology's sustainability was further demonstrated by calculating a series of green metrics. Chapter 3 deals with synthesizing and characterizing novel chiral deep eutectic solvents and their application in asymmetric organocatalysis as combined solvent/organocatalyst. Thus, chiral ammonium salts were prepared and combined with other compounds, forming eutectic mixtures characterized by different procedures and later employed in the enantioselective addition of cyclohexanone to trans-β-nitrostyrene.

Currently, there are environmental issues the world is facing which include global climate change, ozone depletion, pollution, high consumption of resources and increasing amounts of solid waste. Tourism is one of the largest industries and it is an important economic tool. However, it also has many negative effects on the environment. Hotels as one of the tourism industry’s component parts have a significant impact on the environment. From an environmental perspective, hotels consume a large amount of resources such as water and electricity as they make efforts to provide physical comfort to guests. In addition, hotels generate large quantities of waste, which is one of their most visible effects on the environment. Hotels also make a significant contribution to greenhouse gases which are a cause of concern due to the ozone layer depletion. Many hotel customers have become aware of the environmental damages and wasting of environmental resources caused by hotels and they now look for hotels that follow eco-friendly practices. This has brought increasing pressure on hotels to implement more green initiatives. There is limited documented information on green management among hotels in South Africa and in Zimbabwe. While the number and range of impacts hotels have on the environment suggests an urgent need to address them, the environmental management initiatives in these hotels are scantly addressing these green issues and this study is the first to address these issues. The goal of the study was therefore to develop a green management framework for hotels in South Africa and Zimbabwe so that these developing countries may contribute significantly in mitigation of the negative environmental effects. To achieve this goal, two literature chapters addressed the issue of green management and how it can be implemented by hotels. A qualitative research was then carried out employing the case study strategy to collect data from hotel managers in South Africa and Zimbabwe (four in Zimbabwe and four in South Africa). Data was collected using personal interviews involving a direct meeting of the interviewer and the interviewee. Personal interviews were carried out in Zimbabwe. In South Africa, data was collected using telephone interviews. In both Zimbabwe and South Africa, data was recorded on a digital voice recorder. Data was later transcribed and then analysed using the content analysis method. Some of the main findings of the study are that, while some perceptions of managers about the green management concept are consistent with a number of aspects emphasised in literature, some hotel managers do not fully understand or grasp what the concept of green management entails. This indicates that a gap exists between managers’ knowledge and what green management really means, at least according to literature. The study has also established that in most hotels, there is unavailability of green management policies, suggesting that there is a lack of knowledge about the importance of a green management policy amongst hotel managers in these two developing countries. The key contributions of this study are that, it provides insights on the current debate on green management in hotels as revealed through green management initiatives implemented in hotels in South Africa and Zimbabwe. Secondly, the study makes a contribution by doing a supply side analysis and identifying the views of hotel managers with regard to green management. This study also contributed by proposing a green management framework for hotels – an aspect that is currently lacking in the existing literature especially from a developing country point of view. The green management framework proposed has guidelines for implementation. The hoteliers in the two countries will thus benefit from the framework and will be in a position to implement green initiatives to mitigate the negative impacts hotels have on the environment. / PhD (Tourism Management), North-West University, Potchefstroom Campus, 2015

Green marketing is now recognised amongst academics as a reputable area of study and conventional marketing has taken a step back as green marketing comes into prominence in the fight against unsustainability. A number of organisations are using green marketing as a tool to differentiate their market offerings from those of their competitors in an effort to gain a strong position in today’s markets. These organisations are seeking to exploit consumers‟ growing environmental concerns and increasing green purchase intentions in order to acquire market share in the newly developed green consumer markets. The Generation Y cohort, born between 1986 and 2005, are the most technologically astute generation to date. When segmenting the Generation Y cohort, the black Africans hold the majority share, comprising 84 percent of the Generation Y cohort and approximately 32 percent of the entire South African population. Owing to its sheer size, the black Generation Y cohort presents as an attractive and lucrative market segment, especially those who hold a tertiary education. Individuals who pursue a higher education are linked to higher future earning potential. The primary objective of this study was to propose and empirically test a model of the antecedents of black Generation Y students‟ green purchase behaviour within the South African context. The proposed model suggests that environmental knowledge, subjective norms, and perceived behaviour control have a direct positive influence on environmental attitude, which, in turn, has a direct positive influence on green purchase intentions. Moreover, the model infers that green purchase intensions have a direct positive influence on environmental purchase behaviour, while accounting for the mediating effects of perceived price (price) and perceived quality (quality). The sampling frame for the study comprised the 25 public registered HEIs situated in South Africa. From this initial list of 25 registered institutions, a judgement sample of four institutions in the Gauteng province was chosen, of which two included country-based universities and two city-based universities. Of the four universities, two were traditional universities, one a university of technology and one comprehensive university. Lecturers at each of the four campuses were contacted and asked if they would act as gatekeepers to the student participants. A convenience sample of 500 students across these four campuses was taken in 2014. Of the questionnaires completed, 332 were usable. The statistical analysis of the collected data included exploratory factor analysis, descriptive statistical analysis, correlation analysis, structural equation modelling and independent sample t-tests. The findings of this study indicate that South African black Generation Y students are knowledgeable about the environment, consider the opinions of their peers regarding the environment, perceive their actions as having a positive effect on the environment and display strong pro-environmental attitudes towards the environment. Moreover, they display positive intentions to purchase green products and aim to behave in a pro-environmental manner. The influence of green purchase intentions on green purchase behaviour is partially mediated by the perceived price and quality of green products. This may explain the noticeable gap between environmental awareness and lack of actual green product purchases. Environmental knowledge and perceived behaviour control had a significant direct effect on black Generation Y students‟ environmental attitude, which, in turn, has a significant direct influence on black Generation Y students‟ green purchase intentions. Similarly, subjective norms and environmental knowledge had a significant direct effect on green purchase intentions. This study contributes to developing the green consumer profile of the black Generation Y consumer in South Africa. Furthermore, the study will aid in identifying the green consumer behaviour patterns amongst the South African youth. This study offers a conceptual model that illustrates the antecedents of black Generation Y students‟ green purchasing behaviour. The findings of this study will be helpful to national and international marketers seeking to profile and target the lucrative green market segment in South Africa. / PhD (Marketing Management)--North-West University, Vaal Triangle Campus, 2015

Currently, there are environmental issues the world is facing which include global climate change, ozone depletion, pollution, high consumption of resources and increasing amounts of solid waste. Tourism is one of the largest industries and it is an important economic tool. However, it also has many negative effects on the environment. Hotels as one of the tourism industry’s component parts have a significant impact on the environment. From an environmental perspective, hotels consume a large amount of resources such as water and electricity as they make efforts to provide physical comfort to guests. In addition, hotels generate large quantities of waste, which is one of their most visible effects on the environment. Hotels also make a significant contribution to greenhouse gases which are a cause of concern due to the ozone layer depletion. Many hotel customers have become aware of the environmental damages and wasting of environmental resources caused by hotels and they now look for hotels that follow eco-friendly practices. This has brought increasing pressure on hotels to implement more green initiatives. There is limited documented information on green management among hotels in South Africa and in Zimbabwe. While the number and range of impacts hotels have on the environment suggests an urgent need to address them, the environmental management initiatives in these hotels are scantly addressing these green issues and this study is the first to address these issues. The goal of the study was therefore to develop a green management framework for hotels in South Africa and Zimbabwe so that these developing countries may contribute significantly in mitigation of the negative environmental effects. To achieve this goal, two literature chapters addressed the issue of green management and how it can be implemented by hotels. A qualitative research was then carried out employing the case study strategy to collect data from hotel managers in South Africa and Zimbabwe (four in Zimbabwe and four in South Africa). Data was collected using personal interviews involving a direct meeting of the interviewer and the interviewee. Personal interviews were carried out in Zimbabwe. In South Africa, data was collected using telephone interviews. In both Zimbabwe and South Africa, data was recorded on a digital voice recorder. Data was later transcribed and then analysed using the content analysis method. Some of the main findings of the study are that, while some perceptions of managers about the green management concept are consistent with a number of aspects emphasised in literature, some hotel managers do not fully understand or grasp what the concept of green management entails. This indicates that a gap exists between managers’ knowledge and what green management really means, at least according to literature. The study has also established that in most hotels, there is unavailability of green management policies, suggesting that there is a lack of knowledge about the importance of a green management policy amongst hotel managers in these two developing countries. The key contributions of this study are that, it provides insights on the current debate on green management in hotels as revealed through green management initiatives implemented in hotels in South Africa and Zimbabwe. Secondly, the study makes a contribution by doing a supply side analysis and identifying the views of hotel managers with regard to green management. This study also contributed by proposing a green management framework for hotels – an aspect that is currently lacking in the existing literature especially from a developing country point of view. The green management framework proposed has guidelines for implementation. The hoteliers in the two countries will thus benefit from the framework and will be in a position to implement green initiatives to mitigate the negative impacts hotels have on the environment. / PhD (Tourism Management), North-West University, Potchefstroom Campus, 2015