Hemp is a plant containing many active substances and it is widely used in industry. The most common applications are the food, pharmaceutical, textile and cosmetics industries. This diploma thesis dealt with the characterization of hemp fractions, which are created during hemp processing and their potential applications. The theoretical part was focused on the description of the plant, its chemical composition and the research of the active substances in hemp. Also, its industrial use, the technique of hemp processing and the current applications of individual fractions were summarized. In the experimental part, hemp fractions were analysed in terms of nutrient content. Hemp extracts were prepared using three solvents - hexane, 70% ethanol and distilled water, which were compared based on the extraction yield of polyphenols, flavonoids, antioxidants, chlorophylls and cannabinoids. Antimicrobial effects were analysed in hexane and ethanol extracts. By-products of hemp processing were used in some screening application in biotechnology, cosmetics and nutrition. The results show that the extract with 70% ethanol had the highest content of active substances. From the hemp fractions, hemp trichomes and Fedora flowers had the highest content of active substances. Cannabis by-products have the potential to become valuable substrates for yeast cultivation. Face scrub containing hemp flour as an abrasive component had favourable results and was acceptable to consumers, as was the prepared hemp flour raw bar, which was evaluated to be comparable to commercially available hemp protein raw bars.

Limited information exists regarding the processing parameters and extent of reinforcing potential natural fibers have in polymer matrices. The five natural fiber mats studied were low shive flax, mid shive flax, high shive flax, hemp and kenaf. The parameters characterized were fiber size, wax content, surface contact angle, and shive content. The compressive force and unsaturated permeability was measured for each mat, and composites were constructed and tested using selected mats in a soy-based polyurethane (PU) matrix. All mats exhibited a viscoelastic behavior under compression, and an increase in shive content correlated with an increase in relaxation. The presence of shive and larger fiber size increased the permeability. Higher wax content and contact angle lowered the permeability. The mechanical properties for all composites performed better than the neat PU, showing there was matrix to fiber adhesion and load transfer. Hemp outperformed the other fibers studied in all mechanical tests.

Industrial hemp is legally defined in the United States by the Agriculture Improvement Act of 2018 (2018 Farm Bill) as Cannabis containing <0.3% total tetrahydrocannabinol (THC). The 2018 Farm Bill does not, however, specify standard methods for sample preparation or quantification of cannabinoids (including THC) in Cannabis. Extraction efficiency of phytochemicals is well-known to depend on the solvent and extraction method used. In this project, we evaluated the effect of sample preparation extraction methods on the quantitative analysis of five cannabinoids found in industrial hemp with regulatory or commercial significance: cannabidiol (CBD), cannabidiolic acid (CBDA), delta-9-tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), and cannabinol (CBN). Extraction methods evaluated include: QuEChERS, diethyl ether, ethanol, and methanol. Extracts obtained via these methods were subject to quantitative cannabinoid analysis by UPLC/PDA. Standard curves for quantification of each cannabinoid were constructed using authentic standards for quantification. The concentrations of each cannabinoid in the plant material determined via each of the extraction methods were compared using one-way ANOVA followed by Tukey's HSD (significant difference defined as p <0.05). All extraction methods evaluated returned different concentrations of total THC in the plant material. The QuEChERS extraction resulted in the highest calculated concentrations of THC, THCA and CBDA, reporting three to four times greater than obtained via other extractions evaluated. Classification of the starting plant material as hemp or marijuana depended on the extraction method used. These findings clearly and quantitatively demonstrate the need for standardization of extraction methods for hemp analysis and regulation. / Master of Science in Life Sciences / Hemp is a type of Cannabis plant that produces an insignificant amount of the psychoactive cannabinoid tetrahydrocannabinol (THC). Hemp was federally illegal in the United States until the 2018 Farm Bill differentiated hemp from Marijuana, defining hemp as containing less than 0.3% total THC. Standard methods for cannabinoid testing in hemp have not been defined. In this project, four extraction methods with potential use for sample preparation in hemp analysis were evaluated and compared. The extraction methods evaluated included: ethanol, methanol, QuEChERS, and diethyl ether. The concentrations of cannabinoids in each of the plant extracts were then determined using an appropriate analytical method and authentic standards for Cannabidiol (CBD), Cannabidiolic Acid (CBDA), THC, Tetrahydrocannabinolic Acid (THCA), and Cannabinol (CBN). Total concentrations of each cannabinoid in the plant material were then calculated using each extraction method. All extraction methods evaluated resulted in different concentrations of total THC in the plant material, with QuEChERS resulting in the highest calculated concentrations of THC, THCA, and CBDA. The identify of this plant material as hemp or marijuana depended on the extraction method used. This result is not surprising, as extraction efficiency of phytochemicals is well known to depend on the solvent and extraction method used. Nonetheless, our findings clearly demonstrate the need for standardization of extraction methods for hemp analysis and regulation.

Chronic, low-grade inflammation is a contributing factor in diseases that impact the health and well-being of horses. Pharmaceutical treatments reduce inflammation, but their use results in negative digestive and kidney disturbances. Polyunsaturated fatty acids (PUFA) play a role in mitigating the inflammatory response and are therefore explored as a dietary approach to attenuate inflammation. γ-Linolenic acid (GLA) is a unique PUFA that when supplemented in the diet can increase the production of anti-inflammatory eicosanoids; however, it is uncommon in the dietary components normally fed to horses. Interest in industrial hemp (Cannabis sativa L.) as a novel source of PUFA stems from the presence of GLA and the potential to reduce inflammation; although, concerns over cannabinoid contamination limit its acceptance. Six Thoroughbred geldings were used in a crossover study with two 63-d periods to measure PUFA metabolism, inflammatory biomarkers, and cannabinoid accumulation in response to hempseed oil (HSO) fed to sedentary horses compared to controls (CON). Treatment diets were offered for the first 35 d of each period and then all horses resumed a uniform feeding rate from d 36 to 63. Serum and synovial fluid PUFA reflected dietary intake. GLA was greater in serum (0.465 vs. 0.046; P < 0.0001) and synovial fluid (0.270 vs. 0; P < 0.0001) in horses fed HSO compared to CON. This contributed to greater dihomo-γ-linolenic acid (DGLA) conversion in serum (0.287 vs. 0.195; P < 0.0001) and synovial fluid (0.348 vs. 0.262; P < 0.04) but not arachidonic acid (AA). Serum GLA returned to baseline concentrations by two weeks post-supplementation, but no treatment x time effect was observed for synovial fluid. HSO did not affect FA in muscle; it is likely the length or quantity of supplementation was inadequate to see changes in muscle PUFA. HSO increased serum interleukin 1β (IL1β; P = 0.01) but there was no treatment by time interaction (P = 0.62). No other inflammatory biomarkers were influenced by treatment. Stride length was not affected by HSO supplementation but was inversely correlated (P ≤ 0.01) with synovial fluid prostaglandin E2 (PGE2; r = -0.56), and positively correlated with serum tumor necrosis factor α (TNFα; r = 0.58), serum IL6 (r = 0.61), and serum IL1β (r = 0.65). Cannabinoids were measured in the HSO supplement, but no cannabinoids were detected in plasma or synovial fluid of horses fed HSO when tested to a 50-ppb limit of detection. These results demonstrate the suitability of HSO as a novel source of PUFA and, more specifically, as a source of GLA without further increasing AA, however, implications for its effect on inflammation require further evaluation. / Doctor of Philosophy / Inflammation contributes to diseases in the horses that reduce their health and well-being. Anti-inflammatory drugs reduce inflammation but are associated with negative health effects including gastric ulcer formation and kidney damage. Diet can influence the inflammatory response and is therefore targeted to moderate inflammation. Specific dietary targets include polyunsaturated fatty acids (PUFA). Hempseed (Cannabis sativa L.) oil (HSO) contains a unique and uncommon dietary PUFA, γ-Linolenic acid (GLA), which can increase the production of anti-inflammatory biomolecules. The goal of this research was to measure PUFA accumulation, specifically GLA, in horses fed HSO for 35 d and then clearance for 28 d post-supplementation. Additionally, we looked at inflammatory markers to determine the effect on inflammation in sedentary horses. Finally, we measured cannabinoids to evaluate if the low level of cannabinoid contamination found in HSO transfers to horse plasma and synovial fluid. To accomplish these goals, we conducted a feeding trial from May 2022 to September 2022 using six Thoroughbred geldings in a cross over study with two 63 d periods. HSO was supplemented the first 35 d of each period and then removed. Serum and synovial fluid PUFA reflected dietary PUFA. Inflammatory biomarkers had a mixed response that could be influenced by additional, unknown factors. The low-level of cannabinoids in the HSO supplement were not detected in plasma or synovial fluid. HSO shows promise as a novel source of PUFA, specifically GLA, without concerns of cannabinoid contaminants.

Le béton de chanvre est obtenu par le mélange d'un granulat végétal, constitué de bois de chanvre, et d'un liant. Il confère au mur de bonnes propriétés d'isolation thermique et acoustique, ainsi qu'une régulation de l'humidité. Cependant, des problèmes de non-prise, qui semblent fortement liés aux transferts d'eau dans le béton au cours des premières heures, peuvent parfois se produire. La RMN permet de quantifier l'eau dans le chanvre et dans le liant et donc de décrire et comprendre les transferts au cours de la prise. Nous montrons d'abord que, dans le liant constitué d'un mélange de chaux hydraté et de ciment, la chaux accélère la prise du ciment. Cette prise est d'autant plus inhibée que la quantité de chanvre au contact du ciment est importante. Nous montrons également que l'absorption d'eau par le granulat de chanvre s'étale sur trois jours, et qu'elle se produit en deux phases successives correspondant à l'imprégnation de deux zones du chanvre. L'étude des transferts dans le béton pendant la prise révèle une absorption d'eau rapide par le chanvre initialement, puis un transfert vers le liant. L'étude d'un béton de chanvre "modèle" nous permet d'associer ce transfert au retrait chimique du ciment au cours de la prise / Hemp concrete results from the mix of a vegetal aggregate (hemp shives) and a binder. It provides thermal and acoustic insulation to the wall, as well as a good moisture regulation. However, problems sometimes occur during setting, that seem strongly linked to transfers of water in the concrete in the first hours. NMR allows to quantify water in hemp and in the binder and thus to describe and understand the transfers during setting. We first show that, in the binder consisting of a mixture of hydrated lime and cement, lime accelerates cement hydration. This hydration is inhibited as the amount of hemp in contact with the cement increases. We also show that the absorption of water by hemp shives is takes place during three days, and it occurs in two successive phases corresponding to two areas of hemp that imbibe. The study of transfers in the concrete during the setting shows a rapid water absorption by the hemp initially, followed by a transfer to the binder. The study of a "model" hemp concrete allows us to associate this transfer to the chemical shrinkage of cement during hydration

Le béton de chanvre est obtenu par le mélange d'un granulat végétal, constitué de bois de chanvre, et d'un liant. Il confère au mur de bonnes propriétés d'isolation thermique et acoustique, ainsi qu'une régulation de l'humidité. Cependant, des problèmes de non-prise, qui semblent fortement liés aux transferts d'eau dans le béton au cours des premières heures, peuvent parfois se produire. La RMN permet de quantifier l'eau dans le chanvre et dans le liant et donc de décrire et comprendre les transferts au cours de la prise. Nous montrons d'abord que, dans le liant constitué d'un mélange de chaux hydraté et de ciment, la chaux accélère la prise du ciment. Cette prise est d'autant plus inhibée que la quantité de chanvre au contact du ciment est importante. Nous montrons également que l'absorption d'eau par le granulat de chanvre s'étale sur trois jours, et qu'elle se produit en deux phases successives correspondant à l'imprégnation de deux zones du chanvre. L'étude des transferts dans le béton pendant la prise révèle une absorption d'eau rapide par le chanvre initialement, puis un transfert vers le liant. L'étude d'un béton de chanvre "modèle" nous permet d'associer ce transfert au retrait chimique du ciment au cours de la prise / Hemp concrete results from the mix of a vegetal aggregate (hemp shives) and a binder. It provides thermal and acoustic insulation to the wall, as well as a good moisture regulation. However, problems sometimes occur during setting, that seem strongly linked to transfers of water in the concrete in the first hours. NMR allows to quantify water in hemp and in the binder and thus to describe and understand the transfers during setting. We first show that, in the binder consisting of a mixture of hydrated lime and cement, lime accelerates cement hydration. This hydration is inhibited as the amount of hemp in contact with the cement increases. We also show that the absorption of water by hemp shives is takes place during three days, and it occurs in two successive phases corresponding to two areas of hemp that imbibe. The study of transfers in the concrete during the setting shows a rapid water absorption by the hemp initially, followed by a transfer to the binder. The study of a "model" hemp concrete allows us to associate this transfer to the chemical shrinkage of cement during hydration

The commercial use of hemp fiber in the construction industry within the United States is non-existent. This lack of use is because of State and Federal laws forbidding the growth of hemp in the United States, which has led to a lack of research. Not having an established supply chain for hemp and coupled with limited research has put the United States behind other countries in finding viable options for these renewable resources. This is a study of the performance of raw hemp fibers and processed hemp twine in a cement past mixture subjected to tensile loading. Three water/cement ratios (0.66, 0.49, 0.42) were considered. Replacement of cement with fly ash is also part of the program to see if it affects the performance of the system. A detailed description of the method of applying the tensile load to the micro/macro fibers along with the fixture setup is part of this article. The results of this investigation show the hemp twine and fibers will bond to the cement matrix and they can carry higher tensile loads at higher w/c ratios. This study shows that 30 mm embedment length is best for hemp macro fibers and 20 mm embedment for hemp micro fibers. This study also includes a comparative investigation of the performance of hemp fibers to synthetic and steel fibers added to a concrete mix. This investigation examined the compressive strength of the fiber-reinforced concrete mixes, flexural capacity, ductility, flexural toughness and the effects the fibers have on Young’s modulus of elasticity. All fibers were introduced to the same mix design (w/c = 0.49) with replacement of 25% of cement with fly ash. Hemp micro fibers at the same dosing rate a synthetic micro fibers has a slightly higher toughness and equivalent flexural strength. Hemp macro fibers at a higher dosing rate as compared to synthetic fibers will have similar toughness and equivalent flexural strength. Steel fibers performed better than the synthetic and natural fibers at 28-day compressive strength.

Many technological hurdles remain before a viable commercial magnetic fusion energy reactor can be constructed, including the development of plasma-facing components with long lifetimes that can survive the harsh environment inside a reactor. One such component, the divertor, which maintains the purity of the plasma by removing fusion byproducts from the reactor, must be able to accommodate very large incident heat fluxes of at least 10 MW/m^2 during normal operation. Modular helium-cooled tungsten divertors are one of the leading divertor designs for future commercial fusion reactors, and a number of different candidates have been proposed including the modular He-cooled divertor concept with pin array (HEMP), the modular He-cooled divertor concept with multiple-jet-cooling (HEMJ), and the helium-cooled flat plate (HCFP). These three designs typically operate with helium coolant inlet temperatures of 600 °C and inlet pressures of 10 MPa. Performing experiments at these conditions to evaluate the thermal performance of each design is both challenging and expensive. An alternative, more economical approach for evaluating different designs exploits dynamic similarity. Here, geometrically similar mockups of a single divertor module are tested using coolants at lower temperatures and pressures. Dynamically similar experiments were performed on an HEMP-like divertor with helium and argon at inlet temperatures close to room temperature, inlet pressures below 1.4 MPa, and incident heat fluxes up to 2 MW/m^2. The results are used to predict the maximum heat flux that the divertor can accommodate, and the pumping power as a fraction of incident thermal power, for a given maximum tungsten temperature. A new nondimensional parameter, the thermal conductivity ratio, is introduced in the Nusselt number correlations which accounts for variations in the amount of conduction heat transfer through the walls of the divertor module. Numerical simulations of the HCFP divertor are performed to investigate how the thermal conductivity ratio affects predictions for the maximum heat flux obtained in previous studies. Finally, a helium loop is constructed and used to perform dynamically similar experiments on an HEMJ module at inlet temperatures as high as 300 °C, inlet pressures of 10 MPa, and incident heat fluxes as great as 4.9 MW/m^2. The correlations generated from this work can be used in system codes to determine optimal designs and operating conditions for a variety of fusion reactor designs.

<p>Hemp (Cannabis sativa L.) is an annual crop used to produce a wide range of products including foods, beverages, nutritional supplements, fabrics, and textiles. Hemp has long been conflated with marijuana and has not been grown in the United States for decades. Due to recent legislation, the legal restrictions on growing hemp seem likely to be lifted. However, although interest is high, industrial hemp has not been grown in the U.S. for nearly 80 years and research on virtually all aspects of hemp production in the U.S. is in its infancy. We lack fundamental knowledge regarding cultivar performance, interactions with pests, particularly weeds, and nutrient requirements. Research is needed to address this knowledge gap and potential production issues as well as to determine the attitudes, perceptions and concerns of farmers regarding the potential adoption of this “new” crop. Importantly, research should be conducted before the crop becomes widely available so that farmers can make informed decisions and avoid costly mistakes. My dissertation consists of four chapters. In Chapter 1, I examine the literature for weed management in hemp production and identify research gaps. In Chapter 2, I investigate the complex legal framework that surrounds Cannabisand the resulting complications for hemp production. In Chapter 3, I present research conducted to determine the attitudes, perceptions, interests and concerns of organic farmers regarding the reintroduction and potential adoption of hemp was completed through survey research. Finally, in the fourth chapter, I present research conducted to characterize the growth and phenology of industrial hemp cultivars and identify cultivars suitable for growing conditions in the Midwest, and to determine the effect of delayed planting on the phenology and growth of seed and fiber hemp varieties in the Midwest.</p><p>Weed control and weed management in industrial hemp production is a surprisingly understudied field. Few peer-reviewed field studies on hemp exist on any subject and in particular, weed control and weed management is understudied. Specifically, only three studies designed to address a weed management issues exist in the literature dating back to 1900. Most commodity crops have extensive literature discussing weed management, and such an extensive gap in the hemp literature suggests that research needs to be conducted to determine the impacts of weeds on hemp production. Discrepancies among state laws and current federal drug legislation have created a convoluted, confusing, and impractical framework currently surrounds hemp production in the U.S. The building of pesticide regulation and product safety systems that are specific to the many end uses of Cannabis have yet to occur in the U.S. Interactions between producers, state and federal government, and third-party testing laboratories need to be facilitated to build regulation systems along with educational programs to train growers appropriate best management. Organic farmers are generally considered less risk adverse than the general farming population and often considered early adopters of technology. I surveyed organic farmers in seven Midwestern states and found that 98.5% of the respondents were generally open to new technologies, but that demographics variables explained little of the variation for respondents’ level of innovativeness as well as their openness to hemp.The respondents were generally open to hemp production (88.2% agreed with the statement that they were open to trying hemp production on their farm) and found that attributes of hemp production that conferred relative advantage and were compatible with existing systems were important. Delayed planting of hemp generally reduced the onset and duration of female flowering and the time to seed formation but the magnitude of these effects varied among cultivars. Seed, stalk, and total above ground dry weight yields varied across cultivar and planting date which may have been impacted by inconsistent stand densities stemming from heavy rainfall and wet soils. Results from this dissertation suggest that hemp is an understudied crop in the U.S., but that interest in its production among organic farmers exists. Field results support the importance of both planting date and cultivar for hemp phenology discussed in previous literature and so research needs to be conducted to explore best hemp production practices in the U.S.<br></p><p></p>

Glass fibres are by far the most extensively used fibre reinforcement in thermosetting composites because of their excellent cost-performance ratio. However, glass fibres have some disadvantages such as non- renewability and problems with ultimate disposal at the end of a materials lifetime since they cannot be completely thermally incinerated. The possibility of replacing E-glass fibres with hemp fibres as reinforcement in sheet moulding compounds (SMC) is examined in this thesis. The composites are manufactured with existing SMC processing techniques and similar resin formulation as used in the commercial industry. An attempt is made to enhance/optimise the mechanical properties of hemp/polyester composites. For this the fibre-matrix interface is modified via chemical modifications with alkaline and silane treatments. Influence of hemp fibre volume fraction, calcium carbonate (CaCO3)filler content and fibre-matrix interface modification on the mechanical properties of hemp fibre-mat-reinforced sheet moulding compounds (H-SMC) is studied. The results of H-SMC composites are compared to E-glass fibre-reinforced sheet moulding compounds (G-SMC). In order to get a better insight in the importance of these different parameters for the optimisation of composite performance, the experimental results are compared with theoretical predictions made using modified micromechanical models such as Cox-Krenchel and Kelly- Tyson for random short-fibre-reinforced composites. These models are supplemented with parameters of composite porosity to improve the prediction of natural fibre composite tensile properties. The influence of impact damage on the residual exural strength of the H-SMC composites is investigated to improve the understanding of impact response of natural fibre reinforced composites. The result of penetration and absorbed energies during non-penetrating impact of H-SMC composites are investigated and compared to values for G-SMC. A simple mechanistic model has been developed for H-SMC composites and is used to get an insight into the impact behaviour of these composite as well as to provide a guideline to compare the experimental results with theoretically calculated data. The fracture toughness properties in terms of the critical-stress-intensity factor KIc, and critical strain energy release rate, GIc, of H-SMC and G-SMC composites are studied using the compact tension (CT) method. It was shown that fracture toughness of H-SMC composites is significantly lower than that of glass fibre reinforced composites (G- SMC). However, results show that with an optimum combination of fibre volume fraction, (CaCO3) filler and surface treatment of the hemp fibres can result in H-SMC composites that have fracture toughness properties that can be exploited for low to medium range engineering applications. It is recommended that to further improve the fracture toughness properties of these natural fibre reinforced composites more research needs to be devoted to the optimization of the fibre-matrix interface properties and ways of reducing porosity content in these composites. Finally, environmental impact of H-SMC composite with conventional G-SMC composite for automotive and non-automotive applications was compared. The composites were assumed to be made in a traditional SMC manufacturing method. Two different types of performance requirements; i.e. stiffness and strength were investigated for both the non-automotive and automotive parts. Two different disposal scenarios: landfill and incineration of the SMC product at the end of life was considered. The LCA results demonstrate that the environmental impact of H-SMC composites is lower than the reference G-SMC composites. G-SMC composites have a significantly higher environmental impact on climate change, acidification and fossil fuels than H-SMC composites. Where as H-SMC composites have a much higher impact on land use and ecotoxicity than G-SMC composites.