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Resumo:A HMGB1 pode atuar promovendo uma resposta inflamatória. Desta forma, os objetivos deste estudo foram quantificar sua concentração no plasma e no líquido peritoneal de bezerros com um processo crônico de hérnia umbilical, antes e após a herniorrafia, além de avaliar seu níveis durante paracenteses seriadas utilizadas como meio diagnóstico auxiliar para se avaliar a resposta inflamatória na cavidade abdominal. A presença de hérnia elevou os valores de HMGB1 no plasma e líquido e peritoneal. A herniorrafia não foi estímulo suficiente para aumentar as concentrações desta proteína no sangue. No entanto, no líquido peritoneal houve elevação dos níveis de HMGB1 em 7 a 15 dias após a cirurgia, decorrente do processo inflamatório esperado estabelecido na cavidade abdominal no pós-operatório. A realização de paracenteses seriadas foi capaz de elevar a concentração da HMGB1 apenas no líquido peritoneal, demonstrando um processo inflamatório local discreto, cujos valores retornam aos basais após um intervalo maior entre uma punção e outra. Sendo assim, a presença de hérnia umbilical congênita em bezerros causa reação local e sistêmica alterando os valores de HMGB1 tanto no plasma quanto no líquido peritoneal, enquanto a herniorrafia e a paracenteses seriadas provocam alterações nas concentrações de HMGB1 tardiamente apenas no líquido peritoneal / Abstract:HMGB1 protein may act by promoting an inflammatory response. Thus, the goals of this study were to quantify plasma and peritoneal fluid concentrations of HMGB1 in calves with or without congenital umbilical hernia before and after surgery, as well as assess their levels during serial paracentesis used as an auxiliary diagnostic tool for assessing the inflammatory response in the abdominal cavity. The presence of a congenital umbilical hernia caused increased values of HMGB1 in plasma and peritoneal fluid. The herniorraphy was not a stimulus intense enough to increase the concentration of this protein in the peripheral blood. However, in the peritoneal fluid, HMGB1 levels were increasead from 7 to 15 days after surgery, in response to the inflammatory process established in the abdominal cavity postoperatively. Serial paracentesis caused an increase of HMGB1 levels in the peritoneal fluid only, showing a mild inflammatory process, returning to baseline values after a longer interval between punctures. Thus, the presence of congenital umbilical hernias in calves cause local and systemic changes in HMGB1 levels both in plasma and in the peritoneal fluid while herniorraphy and serial paracentesis cause later changes in HMGB1 levels only in the peritoneal fluid / Orientador:Juliana Regina Peiró / Banca:Alexandre Secorun Borges / Banca:Valéria Marçal Felix de Lima / Mestre

Made available in DSpace on 2014-06-11T19:25:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-04Bitstream added on 2014-06-13T18:29:09Z : No. of bitstreams: 1 000737976.pdf: 485647 bytes, checksum: 9639675857b2f1dccc09e4c061e226ab (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / HMGB1 protein may act by promoting an inflammatory response. Thus, the goals of this study were to quantify plasma and peritoneal fluid concentrations of HMGB1 in calves with or without congenital umbilical hernia before and after surgery, as well as assess their levels during serial paracentesis used as an auxiliary diagnostic tool for assessing the inflammatory response in the abdominal cavity. The presence of a congenital umbilical hernia caused increased values of HMGB1 in plasma and peritoneal fluid. The herniorraphy was not a stimulus intense enough to increase the concentration of this protein in the peripheral blood. However, in the peritoneal fluid, HMGB1 levels were increasead from 7 to 15 days after surgery, in response to the inflammatory process established in the abdominal cavity postoperatively. Serial paracentesis caused an increase of HMGB1 levels in the peritoneal fluid only, showing a mild inflammatory process, returning to baseline values after a longer interval between punctures. Thus, the presence of congenital umbilical hernias in calves cause local and systemic changes in HMGB1 levels both in plasma and in the peritoneal fluid while herniorraphy and serial paracentesis cause later changes in HMGB1 levels only in the peritoneal fluid / FAPESP: 12/00334-3 / FAPESP: 12/08114-2

A hérnia perineal é paratopia comum em cães machos, idosos, não castrados, cuja etiologia ainda não está bem esclarecida. O tratamento definitivo é a herniorrafia, embora a incidência de falha cirúrgica e as recidivas com o método de reparação padrão seja elevado. O objetivo do presente trabalho foi o de comparar duas técnicas de reparação cirúrgica da hérnia perineal unilateral. Para tanto utilizou-se 10 cães triados pelo HOVET-FMVZ/USP e encaminhados ao Serviço de Cirurgia de Pequenos Animais da mesma faculdade. Os animais foram divididos em dois grupos com cinco representantes em cada um. A hérnia perineal foi corrigida pela técnica de elevação do músculo obturador interno em ambos os grupos. O segundo grupo teve reforço no diafragma pélvico com enxerto heterotópico e xenólogo de retalho de pericárdio de eqüino puro sangue inglês conservado em glicerina a 98%, após a técnica de elevação do músculo obturador intern. Os animais foram avaliados por até 90 dias de pós-operatório, mediante análise clínica, ultra-sonografia e radiográfica da região perineal. Os resultados obtidos demonstraram que houve reforço no local da herniorrafia ao se fixar o pericárdio de eqüino conservado em glicerina a 98%, diminuindo a ocorrência de deiscência dos pontos da musculatura ou ruptura do diafragma operado. / The perineal hernia is a common disease in old, not sterilized, male dogs, whose etiology isnt clear, yet. The definitive treatment is the herniorrhaphy, but the surgical failure and the recurrence rate with traditional reapposition technique is high. The purpose of this present study was to compare two techniques of surgical reparation of the unilateral perineal hernia. To achieve this, we used 10 dogs from the HOVET-FMVZ/USP that were lead to the Service of Small Animal Surgery at the same college. The animals were disposed in 2 groups with 5 representatives each. The perineal hernia was operated by the elevation of the internal obturator or transposition thechnique in both groups. One of these groups was reinforced in their pelvic diaphragms with graft heterotopy and xenogenic from Thoroughbred horse equine pericardium conserved in glycerine at 98%, after the elevation of the internal obturator or transposition thechnique. The animals were examined within 90 days after operation, through clinical analysis, ultrasonography and radiography of the perineal local. The results showed that the local of herniorrhaphy had been reforced by implant of graft heterotopy and xenogenic from Thoroughbred, reducing the recidive of hernia perineal.

Resumo:As ativinas são citocinas membros da superfamília do fator de crescimento transformador-β (TGFβ) que podem ser sintetizadas por quase todos os tipos celulares no organismo e assim a maioria dos tecidos têm a capacidade de ser fonte de ativina A na circulação. Desta forma, os objetivos desse estudo foram determinar se as concentrações plasmáticas e peritoneais da ativina A sofrem alterações em bezerros portadores ou não de hérnia umbilical (HU) antes e após a correção cirúrgica e em qual compartimento o seu aumento refletiria melhor e mais rapidamente as alterações inflamatórias, seu papel como indicador da inflamação na cavidade abdominal e sua correlação entre concentrações plasmáticas e peritoneais e ainda estabelecer intervalos de referência para a variável em cada grupo. Foram utilizados vinte bezerros holandeses com (n=10) ou sem HU (n=10), bezerros com HU foram submetidos à herniorrafia. Sangue total e líquido peritoneal foram coletados dos 20 animais para a quantificação da ativina A pelo teste ELISA nos dias 0 (antes da cirurgia) 1, 3, 5, 7 e 15. Dados dos dois grupos foram comparados. As concentrações plasmáticas e peritoneais de ativina A no grupo controle não tiveram alterações ao longo do tempo, porém no grupo experimental houve um aumento da concentração plasmática 15 dias após a herniorrafia em relação ao momento basal. Quando comparados os grupos, as concentrações plasmáticas de ativina A do grupo experimental estavam diminuídas no 3º dia e aumentadas no 15º dia após a cirurgia em relação aos animais do grupo controle, enquanto a concentração peritoneal apresentou-se aumentada 5 dias após a herniorrafia no grupo experimental em relação ao grupo controle / Abstract:Activins are members of the transforming growth factor β (TGF-β) superfamily and can be synthesized by almost all cell types in the body and most tissues has the ability to be a source of activin A in circulation. Thus, the objectives of this study were to determine if plasma and peritoneal concentrations of activin A change in calves with or without umbilical hernia (UH) before and after surgery and in which compartment its increase would reflect better and faster inflammatory changes. Its role as an inflammatory biomarker in the abdominal cavity and the correlation between plasma and peritoneal concentrations, and establish reference intervals for the variable in each group. Twenty Holstein calves were used with (n = 10) or without HU (n = 10), calves with HU underwent herniorrhaphy. Whole blood and peritoneal fluid were collected from 20 animals for quantification of activin A by ELISA on days 0 (before surgery) 1, 3, 5, 7 and 15. Data were compared between the two groups. Plasma and peritoneal concentrations of activin A in the control group did not change over time, but in the experimental group there was an increase in the plasma concentration 15 days after herniorrhaphy compared to baseline. When comparing the groups, the plasma concentrations of activin A in the experimental group were decreased on day 3 and increased on the 15th day after surgery compared to the control group animals, while the peritoneal concentration had increased 5 days after herniorrhaphy repair in the experimental group compared to the control group / Orientador:Juliana Regina Peiró / Banca:José Paes de Oliveira Filho / Banca:Alexandre Secorun Borges / Mestre

Made available in DSpace on 2016-09-27T13:40:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-01-21. Added 1 bitstream(s) on 2016-09-27T13:45:13Z : No. of bitstreams: 1 000870622.pdf: 409692 bytes, checksum: e09e8f69dfbc508854b5fc39b85dc96c (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Activins are members of the transforming growth factor β (TGF-β) superfamily and can be synthesized by almost all cell types in the body and most tissues has the ability to be a source of activin A in circulation. Thus, the objectives of this study were to determine if plasma and peritoneal concentrations of activin A change in calves with or without umbilical hernia (UH) before and after surgery and in which compartment its increase would reflect better and faster inflammatory changes. Its role as an inflammatory biomarker in the abdominal cavity and the correlation between plasma and peritoneal concentrations, and establish reference intervals for the variable in each group. Twenty Holstein calves were used with (n = 10) or without HU (n = 10), calves with HU underwent herniorrhaphy. Whole blood and peritoneal fluid were collected from 20 animals for quantification of activin A by ELISA on days 0 (before surgery) 1, 3, 5, 7 and 15. Data were compared between the two groups. Plasma and peritoneal concentrations of activin A in the control group did not change over time, but in the experimental group there was an increase in the plasma concentration 15 days after herniorrhaphy compared to baseline. When comparing the groups, the plasma concentrations of activin A in the experimental group were decreased on day 3 and increased on the 15th day after surgery compared to the control group animals, while the peritoneal concentration had increased 5 days after herniorrhaphy repair in the experimental group compared to the control group

A hérnia perineal é paratopia comum em cães machos, idosos, não castrados, cuja etiologia ainda não está bem esclarecida. O tratamento definitivo é a herniorrafia, embora a incidência de falha cirúrgica e as recidivas com o método de reparação padrão seja elevado. O objetivo do presente trabalho foi o de comparar duas técnicas de reparação cirúrgica da hérnia perineal unilateral. Para tanto utilizou-se 10 cães triados pelo HOVET-FMVZ/USP e encaminhados ao Serviço de Cirurgia de Pequenos Animais da mesma faculdade. Os animais foram divididos em dois grupos com cinco representantes em cada um. A hérnia perineal foi corrigida pela técnica de elevação do músculo obturador interno em ambos os grupos. O segundo grupo teve reforço no diafragma pélvico com enxerto heterotópico e xenólogo de retalho de pericárdio de eqüino puro sangue inglês conservado em glicerina a 98%, após a técnica de elevação do músculo obturador intern. Os animais foram avaliados por até 90 dias de pós-operatório, mediante análise clínica, ultra-sonografia e radiográfica da região perineal. Os resultados obtidos demonstraram que houve reforço no local da herniorrafia ao se fixar o pericárdio de eqüino conservado em glicerina a 98%, diminuindo a ocorrência de deiscência dos pontos da musculatura ou ruptura do diafragma operado. / The perineal hernia is a common disease in old, not sterilized, male dogs, whose etiology isnt clear, yet. The definitive treatment is the herniorrhaphy, but the surgical failure and the recurrence rate with traditional reapposition technique is high. The purpose of this present study was to compare two techniques of surgical reparation of the unilateral perineal hernia. To achieve this, we used 10 dogs from the HOVET-FMVZ/USP that were lead to the Service of Small Animal Surgery at the same college. The animals were disposed in 2 groups with 5 representatives each. The perineal hernia was operated by the elevation of the internal obturator or transposition thechnique in both groups. One of these groups was reinforced in their pelvic diaphragms with graft heterotopy and xenogenic from Thoroughbred horse equine pericardium conserved in glycerine at 98%, after the elevation of the internal obturator or transposition thechnique. The animals were examined within 90 days after operation, through clinical analysis, ultrasonography and radiography of the perineal local. The results showed that the local of herniorrhaphy had been reforced by implant of graft heterotopy and xenogenic from Thoroughbred, reducing the recidive of hernia perineal.