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No description available.

Insulin-like growth factor 1 (IGF-1) acts as a mediator for several hormones in bone. In rat the IGF-1 gene is processed into distinct mRNA molecules which differ in their 5' and 3' ends. Two major classes of mRNA transcript are produced which contain either exon 1 (Class 1) or exon 2 (Class 2). The main aims of this study were to identify the IGF-1 splice variants produced in bone and investigate possible effects of GH on transcript expression. IGF-1 transcripts were analysed in bone using reverse transcription polymerase chain reaction (RT-PCR) using forward specific primers for exon 1 and exon 2 and reverse primers for exon 5 and 6. Analysis of RNA extracted from either whole tibia, growth plate, primary osteoblasts, and clonal cell lines UMR 106 and ROS 17.6 showed distinct patterns of expression. All IGF-1 splice variants were expressed in bone tissue and primary osteoblasts however none of the variants were detected in UMR 106 cell line and only Class 1 Ea was detected in ROS 17.6 cell line. No obvious change in this pattern of expression was observed on GH stimulation of these primary cells and osteosarcoma cell lines. However, GH was found to stimulate osteoblast growth by enhancing proliferation of a subpopulation of cells but GH did not effect alkaline phosphatase production. GH did not increase total IGF-1 mRNA levels in osteoblasts as detected using RNase Protection assay but there was a change in the splicing profile such that Class 2 transcripts were increased by 150% (p=0.05) and Class 1 transcripts decreased by 35% (p=0.02). Furthermore antisense ODNs directed against the common exon 4 ofIGF-1 caused a dramatic increase in osteoblast apoptosis whereas sense and scrambled control ODNs had no effect. Our data show that IGF-1 is an important constitutively expressed factor in osteoblasts and GH may exert its actions by increasing Class 2 transcripts in bone which could have paracrine effects on neighbouring cells.

Call number: LD2668 .T4 1950 N8 / Master of Science

The animal kingdom contains a spectacular diversity in colour signals used to indicate quality. The challenge of understanding this diversity lies in identifying and interpreting constraints on signals that maintain signal honesty. I used an integrative approach to measure the effects of potential signal modulators on whole-animal performance, ornaments, condition and immunocompetence in the lizard P. i. wilhelmi. This approach attempts to remove some of the uncertainty surrounding the validity of existing handicap models. First, I investigated seasonal changes in testosterone, corticosterone and carotenoids and compared these to seasonal changes in endurance, immunocompetence and body condition male P. i. wilhelmi. I also determined which colour patches were predictors of male quality by relating them to morphology, endurance, body condition and immunocompetence. I found some support for the immunocompetence handicap hypothesis so I tested whether testosterone was modulating ornaments and constraining signals through its immunosuppressive properties. I tested the immunocompetencehandicap hypothesis in male P. i. wilhelmi while also conducting parallel studies with free-ranging and captive-housed lizards to assess whether there were differences between the groups that could indicate exogenous factors influencing signalling in their natural environment. While experimentally elevated testosterone did affect endurance and the properties of their colour patches, immunosuppression was only evident in free-ranging lizards. To measure the extent of organisational effects in males I also manipulated testosterone in females and found no evidence of immunosuppression although testosterone did affect some aspects of colouration and endurance. Next, I tested the stress-linked immunocompetence handicap hypothesis by experimentally elevating testosterone, corticosterone, or both at the same time. I found that corticosterone had an isolated effect on one colour patch, and testosterone and corticosterone had opposing effects on endurance that were negated when both were elevated. Different colour patches were affected by either testosterone or corticosterone with little overlap, and the combination of the two had a different action to either hormone elevated in isolation. Finally, I tested the oxidative stress hypothesis of carotenoids as a limiter of signal output due to their requirement in the oxidative stress response. I found that supplemented carotenoids vastly improved endurance, immune response and influenced different aspects of colouration to that of testosterone and corticosterone. My study examined four different hypotheses of constraints on signalling involving testosterone, corticosterone, interactions between testosterone and corticosterone and carotenoids in a consistent manner measuring multiple indices of quality as well as multiple colour signals. This study provides a unique integrative perspective on the roles played by each factor as well as prompting us to re-examine our approach to understanding constraints on signalling.

by Lau Kwai-Ling. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1980. / Bibliography: leaves 75-83.