This diploma thesis deals with the preparation of solid hydrophilic sponges made of acetalized PVAl. The sponges were produced by foaming by gaseous reaction by-product. The experimental work also includes the study of active compound immobilization into the sponge (finely ground lignite as a heavy metal sorbent).

This master‘s thesis concerns with occurrence of heavy metals in the environment, mainly in the matrices of bioindicators. Special attention is given to occurrence of mercury in the environment and its quantification in bioindicators. Mercury and its compounds are today considered as one of the most significant contaminants in the environment. Some sorts of mushrooms cumulate sizable amounts of mercury in their fruiting body and therefore we can use them to consider loading of different areas. Eight areas in Vizovice and its surroundings were chosen for these purposes and during the years 2006 – 2008 harvested 50 samples from 19 sorts of mushrooms from there. The concentrations of Hg were determined in all samples by single-purpose absorption spectrometer AMA 254. Comparison of the mercury content between individual sorts of mushrooms in the same area as well as comparison of loading of areas was performed and also bioaccumulation ability of individual sorts of mushrooms was determined.

A microscopic fungus Pseudogymnoascus destructans (Ascomycota: Pseudeurotiaceae) causes illness known as white-nose syndrome (WNS) causing death of bats during hibernation. The illness occurs in the North America and in Europe. The fungus is characteristic by asymmetrically curved conidia, by slow growth and growth at low temperatures (below 20 řC). The aim of this study is to clarify properties responsible for unique ecelogy of Pseudogymnoascus destructans by comparison with ecological related or unrelated pathogenic or nonpathogenic fungi. This part includes study of tolerance to physiological stresses and recognition of spectrum of utilizating nutrients (compounds of carbon, nitrogen, phosphorus, sulphur and nutrient supplements). Testing to physiological stresses should help to estimate a potentiality of fungus to spread out of caves. The last aim is to develop a selective isolation medium for P. destructans. Influence of several types of physiological stress (e.g. UVA, UVA with UVB, 25 řC, 30 řC, 37 řC and dryness) was investigated with fluorescent stain propidium iodide (PI) by flow cytometry. The spores of Pseudogymnoascus destructans and three fungi from underground spaces were not viable after 3 weeks at 37 řC. Other stresses did not cause a decreasing of viability or some stresses caused...

This diploma thesis deals with the decomposition of fungal necromass in the Arctic tundra (Svalbard archipelago) under the factor of climate change (simulated by an increased temperature inside the Open Top Chamber). The dynamics of fungal necromass decomposition of two selected fungi, which differ in the level of melanin content and in C:N ratio - Laccaria laccata (hyaline, lower C:N ratio) and Phialocephala fortinii (melanized, higher C:N ratio), was compared. The aim of the work was to evaluate the influence of melanization level of fungal necromass and elevated temperature on the dynamics of fungal necromass decomposition and on the community composition of the decomposers (fungi, bacteria). The experiment focused on monitoring the dynamics of fungal necromass decomposition, changes in enzyme activity, changes in melanin content and C:N ratio during decomposition, as well as on the analysis of the microbial community composition on decomposing mycelium. Throughout the whole incubation, the necromass of P. fortinii decomposed more slowly than the necromass of L. laccata. The differences in the dynamics of decomposition were mainly due to the biochemical composition of the fungal necromass (C:N ratio and melanin content). The melanin content increased in both types of mycelium during...

In a three-year field experiment we evaluated the effect of arbuscular mycorrhizal fungi (AMF) on physiological processes and fertility of grapevine (Vitis vinifera L.), there were two variants: inoculated with AMF compared to uninoculated control. The experiment took place at the Faculty of Horticulture, Mendel University in Brno, using Pinot noir and Lemberger planted as a field experiment and in containers (seedlings) and the second part took place in already established vineyard (mature plants). The positive effect of AMF on the must gained from mature plants of both varieties was observed at following parameters: sugar content, assimilable N, Mg and Fe. All the seedlings from both container and field experiment and both varieties demonstrated a positive effect of AMF on the intensity of photosynthesis, stomatal conductance and transpiration level. The expected positive impact of AMF on all investigated parameters was not significatntly demonstrated, which corresponds with the results of current research on this topic.

This dissertation deals with the use of arbuscular mycorrhizal fungi in vegetable production. The completion of knowledge and confirmation of scientific hypothesis in practise is necessary to be done for each vegetable species. For these reasons the dissertation establishes the entire growing process of the leek (Allium porrum L.). The experiment explores the influence of arbuscular mycorrhizal fungi on the physiological, nutritional and yield parameters of the leek crop. The literary review focuses on the mycorrhizal symbiosis as entire complex and shows an arbuscular mycorrhizal symbiosis, its basic function, usage and impact on plants. Several parts of literary review mention the transfer of elements from the soil to the roots of plants by arbuscular mycorrhizal fungi and currently valid nomenclature is also listed. The experimental part is focused on a trial with several species of arbuscular mycorrhizal fungi and their combinations. The morphological evaluation of pre-cultivated leeks and also field grown plants was focused on the comparison of length and weight of the plants. Nutritional assessments are focused primarily on the vitamin C, total antioxidant capacity, content of nitrates, content of phosphorus and nitrogen in plant tissues. In the pursuance to our results, it is possible to say, that certain variants of arbuscular mycorrhizal fungi had positive influence on the height of pre-cultivated leeks, and a positive trend was also confirmed during the evaluation of the content of antioxidants. The amount of heavy metals in plant tissues was reduced by combination of fungi R. intraradices and C. claroideum. The positive influence of inoculation was also observed at the reduction of nitrates in 2014. Overall, we can say that using of arbuscular mycorrhizal fungi in the cultivation of leeks (Allium porrum L.) contribute to a positive effect on plants and yield, especially in the years with extreme weather conditions during growing season.

In the leading part of this work I presented general information on the kingdom of fungi and fungal ecology focused on marofungi (macromycetes). Furthermore, I reviewed the phenomenon of trace elements accumulation in macrofungal fruit-bodies, particularly focused on uranium and its biogeochemistry. In the experimental part, results of my own research are presented. I determined concentrations of uranium, thorium, silver and lead by use of HR- ICP-MS and ENAA. The macrofungal samples were collected 1/ in clean areas with diverse geological bedrock, 2/ in uranium-polluted area in vicinity of Příbram (Central Bohemia, Czech Republic). Furthermore, selected trace elements were determined also in underlying soils by use of the both analytical methods. For investigation of trace elements mobility, the BCR sequential extraction was applied.

Filamentous microfungi play an important role in the environment and they can have either a positive impact on a man, e.g. in a form of medicine or food production, or a negative one, when filamentous microfungi may cause diseases or deteriorate food. Pupils usually start learning about filamentous microfungi in form 6 of a primary school, or they get some tangential information in the first year of an eight-year grammar school. In addition, in most of student's books, microfungi are incorrectly referred to as moulds, which is an unprofessional term. The objective of my thesis is to elaborate two practical tasks for the presence form of study in which pupils find out the difference between unicellular and multicellular fungal organisms, acquaint themselves with the fungal phytoparasite - mildew, take the substrate, make native and coloured mounts which are then observed under the microscope. The other two practical tasks have been elaborated for the distance form of study via exploration tasks, which can be carried out at home conditions. It means creation of a mycological garden and observation what kind of bakery products is more prone to be affected by microfungi. The other elaborated tasks relate to across-the-curriculum use of the filamentous microfungi topic in Maths and Czech, in which some...

This M. Sc. thesis is based on using of mycoparasitic fungi Trichoderma virens, Clonostachys rosea f. catenulata in biological control against phytopathogenic fungi Sclerotinia sclerotiorum, Rhizoctonia solani, Botrytis cinerea and Fusarium solani. The efficacy of mycoparasitic fungi against pathogens was evaluated in dual cultural tests. The strains of T. virens and C. rosea f. catenulata isolated from soils in the Czech Republic were tested in the experiment. Reference strain was GL 21 fungus T. virens reisolated from commercially available bio-preparation SoilGard and strain C. rosea f. catenulata reisolated from Prestop Mix. All the strains were tested for biological and production properties. All strains are able to colonize the substrate and to suppress the growth and development of pathogens. Strain GL 21 of T. virens was used for seed coating of variety Scirocco in combination with products Guar gum and Carboxymethyl cellulose, which served as a carrier for stick on conidia. After 3 days, the effect of fungus T. virens was evaluated on energy of germination, development of roots of grain. The grain health was determined after 7 days. The fungus T. virens has a positive effect on the grains germination and grain health. During the vegetation the influence of seed coating by T. virens was observed on growth and development of spring wheat. The parameters such as number of plants per m2, tiller numbers, plants health, stand height, number of grains in the spike and thousand grain weight (TGW) were evaluated. During the vegetation the fungus T. virens has positive effect on the plant height.

in English β-N-Acetylhexosaminidase (EC 3.2.1.52) is exoglycosidase, which exhibits the unique properties in the filamentous fungi. Enzyme from these organisms are dimeric, inducible and secreted extracelluary. It is expresed as preproprotein, consists of a signal sequence, a large propeptid and a catalytic subunit. Although the enzyme is widely distributed, its structure differs in varies organisms. Bacteria have only monomeric hexosaminidase. Human enzymes are dimeric as well as fungal, but only hexosaminidase from filamentous fungi have the catalytic subunit noncovalently associated with the propeptide. Propeptide is a essential for the enzyme activity. It exists a homologues model of the catalytic subunit of β-N-acetylhexosaminidase from Penicillium oxalicum, but the structure of the propeptide has not yet been solved. The first part of this diploma thesis deals with the optimization of production and purification conditions. The second part deals with structural studies of β-N-acetylhexosaminidases from the filamentous fungi Penicillium oxalicum CCF 3438. These studies were carried out using chemical cross-linking and high resolution mass spectrometry. The combination of these methods revealed region of the noncovalent interaction of the catalytic subunit with the propeptide.