This thesis is focused on evaluation of the compatibility of entomopathogenic fungus Isaria fumosorosea with different species of entomopathogenic fungi and mycoparasitic fungi at different temperatures. The strains of species Beauveria bassiana, Metarhizium anisopliae, Lecanicillium muscarium, Coniothyrium minitans and Clonostachys rosea f. catenulata were selected for experiments base on compatibility. The results showed that combination of I. fumosorosea with species L. muscarium is compatible. The species do not limit to each other in the environment at all temperatures (15, 23 and 25 °C). On the other side, fungus I. fumosorosea in combination with other species have affected their growth and spore production. The efficacy of entomopathogenic fungi against larvae Tenebrio molitor was evaluated. The most effective species against larvae were species I. fumosorosea, B. bassiana and M. anisopliae. On the contrary, the smallest effective was observed after infection larvae by L. muscarium. Mycoparasitic fungus C. rosea f. catenulata was not able to directly infect larvae of T. molitor. This species did not infect healthy larvae. However it is able to infect weakened individuals or is growing as saprotrophs on the cadavers.

Diploma Thesis concerns with usage of entomopathogenic fungi in system of biological control against greenhouse whitefly Trialeurodoes vaporariorum and sweetpotato whitefly Bemisia tabaci. The Aim of diploma thesis was through in vivo and in vitro experiments determinated the basic evolutional and vegetative characteristics of selected entomopathogenic fungi and compare their effectivity in completed system {\clqq}plant - pest - pathogenic``. All experiments and results are demonstrated in the tables and the graphs. The photo-documentation of the method used during founding and interpretation of experiments is the part of the thesis.

The potential synergism between chosen species of entomopathogenic nematodes (Steinernema arenarium, Steinernema feltiae) and entomopathogenic fungi (Beauveria bassiana, Isaria fumosorosea, Lecanicillium lecanii) is investigated in this study. It is theoretically possible to expect increasing of their efficiency at the collective introduction into environment. Creating of uniform laboratory method which will be possible to use at the research of this interaction system is a part of this study.

This graduation theses was intent on study influence conditions submerged cultivation select types enthomopatogenic fungi in liquid nutritive medium with emphasis on optimalization key elements of the process that manner performance uniform biomass mythosporotic fungi {--} blastospores. Experimental part of work was conceived with regard on next sphere problems: 1.Nutritive soil compositionon effect on production blastospores 2.Comparing possibility different kinds and strains enthomopathogenic fungi produce blastospores in submerged cultivation. 3.Conditions submerged cultivation effect on production and yield blastospores 4.Verify possibility production of uniform biomass blastospores in range usable for large-screen application

Beauveria bassiana is used in biological control against the economically significant pests. Detection of morphological and genetic polymorphism between different species and strains isolated in the natural environment is important to study the distribution and effects in the ecosystem. The aim of this study was to determine in which links are "local tribes" with ecological aspects of biological control in protected zone and assess the polymorphism of the sections by using molecular markers. Were analyzed 36 strains collected in the National Park Šumava in the Czech Republic. Polymorphism of these strains was determined according to a sequence of LSU (28S Large Subunit Ribosomal DNA). It is observed that the populations from National Park is closed, "the local tribes" because these methods can be very well characterized and compared with other strains from different parts of the Czech Republic and other countries. Populations from National Park showed no differences in polymorphism. Through this analysis, the sample Bba I101 was aligned. This preparation is re-used for bark beetle occurring in the National Park Šumava in the Czech Republic. This study was supported by grants GACR 521/08/H042, MSM 60076658-06, MGA SP/2d1/41/08.

This M.Sc. thesis is aimed to describe growth and production parameters of nematophagous fungus Arthrobotrys oligospora. There was determinated information about growth and sporulation of fungus on artificial and natural substrates, influence of cultivation condition on production biomass and possibility of formulation alginate pellets.

Many organic compounds are released to the environment and can be harmful to living organisms. These compounds are often persistent and toxic. Some are mutagens, carcinogens, endocrine disruptors or they can cause an increase in bacterial resistance. They tend to accumulate in nature and their transformation is a long-term process. Therefore, various remediation techniques are needed for decontamination. Remediation and bioremediation processes depend on many factors which should be critically evaluated. This dissertation thesis studies the relationship between bioavailability, biodegradation and toxicity of polychlorinated biphenyls, polycyclic aromatic hydrocarbons and fluoroquinolone antibiotics. These compounds of different origin, character and properties were degradated by ligninolytic fungi. Desorption behaviour of pollutants from historically contaminated sites, degradation potential of ligninolytic fungi, ongoing degradation mechanisms, transformation products and their toxicity were studied as important factors for evaluation of mycoremediation and its environmental impact. The results show that determination of bioaccessible fraction by sequential supercritical fluid extraction is very useful for precise prediction of biodegradability of pollutants. The evidence that ecotoxicity and...

Polychlorinated biphenyls (PCBs) are a class of important organic pollutants which undergo very slow degradation and tend to persist for a long time in the environment. PCBs have various negative effects on living organisms, human health and the environment in general. A method for determining PCB levels in aqueous matrices was developed consisting of solid-phase extraction (SPE) and GC/MS determination. The average recoveries of PCB congeners from artificially spiked deionized water were around 85%. The PCB concentration of 3.3±0.1 μg·l-1 (a sum of selected congeners) was detected in contaminated groundwater originated from the area of a former asphalt concrete producing plant. The most abundant PCBs were trichlorinated congeners which together comprised about 80% of the total PCB content. The optimised SPE method was further applied together with accelerated solvent extraction (ASE) to assess the degradation potential of oyster mushroom (Pleurotus ostreatus) which shows high degradation efficiency of various organic pollutants including PCBs. P. ostreatus belongs to ligninolytic (white-rot) fungi, which is a very promising group of microorganisms frequently studied due to their bioremediation potential. P. ostreatus, strain KRYOS was able to remove 41±8% of the initial amount (50 μg) of the...

The topic of the diploma thesis was the study of the influence of commercial mycorrhizal preparation on the rooting of softwood cuttings of two selected species of ornamental shrubs (Forsythia × intermedia 'Maluch' and Weigela florida 'Piccolo'). The main objective was to find out whether the mycorrhizal preparation can make the process of vegetative propagation more efficient and favourably influence the formation of roots. The used preparation contained arbuscular mycorrhizal fungi of the genus Glomus, two different amounts of the preparation were used. The experiment was carried out in the summer months of the year 2016. The experiment was founded in a plastic greenhouse on the ground of the Faculty of Horticulture in Lednice in Moravia (the Czech Republic), two main terms of propagation passed (in June and August) and one supplementary term passed (in August). The total number of adventitious roots, the number of branched roots, the length of shoots and the mortality of cuttings were evaluated. Mycorrhizal colonization of roots was evaluated in the laboratory under the microscope. The results of the experiment did not show that the used mycorrhizal preparation can positively affect the rooting of softwood cuttings, the application of the mycorrhizal inoculum did not improve the efficiency of vegetative propagation and did not provide convincing advantages. Mycorrhizal fungi did not stimulate the growth of cuttings, and in fact they did not influence the number of adventitious roots neither in Forsythia × intermedia 'Maluch', nor in Weigela florida 'Piccolo'. The only significant positive was that the Weigela florida 'Piccolo' had a higher number of branched roots after inoculation. The observation of roots under the microscope showed that the roots of both species were colonized by arbuscular mycorrhizal fungi, but the extent of colonization did not reach high levels. Mycorrhizal inoculation of propagation substrate cannot be recommended as an effective method that would improve rooting. However, it should be added that the experiment only passed for one year and the results were based only on one term of propagation. Thus the influence of mycorrhizal fungi on the rooting of plant cuttings should be the subject of further research.

In this work is discussed about material Lignamon, which has been industrially produced in the former Czechoslovakia since the 1970s. The production of this material, mainly from beechwood, combines plasticization with gaseous ammonia, densification of material by hydraulic press and heat stabilization at 180 ° C. The aim of this work is to determine the effect of these processes on the resulting resistance to wood-decaying fungi. Lignamon has a very high resistance to wood-decaying fungi and is classified as very durable according to EN 350 - 1. For each phase of the Lignamon process, samples were produced to best illustrate the changes that take place during the given phase in the wood. In particular, sample sets treated with ammonia gas, densified samples, heat-treated samples, and samples on which a combination of ammonia gas impregnation and thermal modification were performed. The resistance to wood-decaying fungi have been tested according to EN 113, including a set of Lignamon samples. The results of this test were statistically evaluated and it was found that ammonia treatment has the greatest influence on the durability of Lignamon, as well as the heat treatment and not so significantly densification. Individually, however, none of these modifications cause wood resistance to wood-decaying fungi as their combination in the production process of Lignamon.