The domain of medical imaging analysis has burgeoned in recent years due to the availability and affordability of digital radiographic imaging equipment and associated algorithms and, as such, there has been significant activity in the automation of the medical diagnostic process. One such process, cephalometric analysis, is manually intensive and it can take an experienced orthodontist thirty minutes to analyse one radiology image. This thesis describes an approach, based on genetic programming, neural networks and machine learning, to automate this process. A cephalometric analysis involves locating a number of points in an X-ray and determining the linear and angular relationships between them. If the points can be located accurately enough, the rest of the analysis is straightforward. The investigative steps undertaken were as follows: Firstly, a previously published method, which was claimed to be domain independent, was implemented and tested on a selection of landmarks, ranging from easy to very difficult. These included the menton, upper lip, incisal upper incisor, nose tip and sella landmarks. The method used pixel values, and pixel statistics (mean and standard deviation) of pre-determined regions as inputs to a genetic programming detector. This approach proved unsatisfactory and the second part of the investigation focused on alternative handcrafted features sets and fitness measures. This proved to be much more successful and the third part of the investigation involved using pulse coupled neural networks to replace the handcrafted features with learned ones. The fourth and final stage involved an analysis of the evolved programs to determine whether reasonable algorithms had been evolved and not just random artefacts learnt from the training images. A significant finding from the investigative steps was that the new domain independent approach, using pulse coupled neural networks and genetic programming to evolve programs, was as good as or even better than one using the handcrafted features. The advantage of this finding is that little domain knowledge is required, thus obviating the requirement to manually generate handcrafted features. The investigation revealed that some of the easy landmarks could be found with 100\% accuracy while the accuracy of finding the most difficult ones was around 78\%. An extensive analysis of evolved programs revealed underlying regularities that were captured during the evolutionary process. Even though the evolutionary process took different routes and a diverse range of programs was evolved, many of the programs with an acceptable detection rate implemented algorithms with similar characteristics. The major outcome of this work is that the method described in this thesis could be used as the basis of an automated system. The orthodontist would be required to manually correct a few errors before completing the analysis.

The objectives for the first part of this thesis were to describe changes in oxygen saturation (SpO2) and heart rate (HR) in newly born infants in the delivery room (DR) and to illustrate the changes using centile reference charts. The objective of the second part of the thesis was to investigate whether infants < 29 weeks gestation who receive positive pressure ventilation (PPV), immediately after birth with a T-piece have higher SpO2 measurements at five minutes than infants ventilated with a self inflating bag (SIB). / Study Design. A prospective observational study was used to achieve the first objectives. For the second part of the thesis I coordinated a randomised, controlled trial of two devices used for resuscitation of extremely preterm infants in the DR where the primary outcome measure was SpO2. / Patients and methods. In all studies a Masimo Radical pulse oximeter (PO) was placed on the infant’s right hand/wrist immediately after birth. PO data (oxygen saturation, HR and signal quality) were downloaded every 2 sec and analysed only when the signal had no alarm messages (low IQ signal, low perfusion, sensor off, ambient light). / Results. Observational studies: The dataset to develop the reference range charts included 61,650 data points from 468 infants. Infants had a mean (range) gestational age of 38 (25-42) weeks and birthweight 2970 (625-5135) g. For all 468 infants at one minute the 3rd, 10th, 50th, 90th and 97th centiles were 29%, 39%, 66%, 87% and 92%; at two minutes 34%, 46%, 73%, 91% and 95% and at five minutes 59%, 73%, 89%, 97% and 98%. It took a median of 7.9 (IQR 5.0 to 10) minutes to reach a SpO2 > 90%. SpO2 of preterm infants rose more slowly than that of term infants. At one min the median (IQR) HR was 82 (66 to 138) bpm rising at two min and five min to 151 (112 to 169) bpm and 166 (148 to 176) bpm respectively. In preterm infants, the SpO2 and HR rose more slowly than term infants. / Randomised trial: Forty nine infants were randomly allocated to the T-piece and 50 to the SIB. Ten infants did not receive PPV, 4 (8%) in the T-piece group and 6 (12%) in the SIB group and were not included in the analysis. Forty-one infants received PPV with a T-piece and 39 with a SIB. At 5 minutes after birth there was no significant difference between the mean (SD) SpO2 in the T-piece and SIB groups [50 (31)% vs. 53 (25)%, (p=0.73)]. More T-piece infants received oxygen during DR resuscitation (100% vs. 90%, p=0.04). There was no significant difference between the groups in the use of continuous positive airway pressure (CPAP); endotracheal intubation or administration of surfactant in the DR. Fewer of the T-piece group who left the DR on CPAP were intubated in the first 24 hrs after birth. (7% vs. 23%, p=0.05). / Conclusion. The centile charts developed in this thesis provide a reference range for SpO2 and HR in the first 10 minutes after birth for preterm and term infants. In the randomised trial there was no significant difference in SpO2 at five minutes after birth in extremely preterm infants given PPV with a T-piece or a SIB.

The objectives for the first part of this thesis were to describe changes in oxygen saturation (SpO2) and heart rate (HR) in newly born infants in the delivery room (DR) and to illustrate the changes using centile reference charts. The objective of the second part of the thesis was to investigate whether infants < 29 weeks gestation who receive positive pressure ventilation (PPV), immediately after birth with a T-piece have higher SpO2 measurements at five minutes than infants ventilated with a self inflating bag (SIB). / Study Design. A prospective observational study was used to achieve the first objectives. For the second part of the thesis I coordinated a randomised, controlled trial of two devices used for resuscitation of extremely preterm infants in the DR where the primary outcome measure was SpO2. / Patients and methods. In all studies a Masimo Radical pulse oximeter (PO) was placed on the infant’s right hand/wrist immediately after birth. PO data (oxygen saturation, HR and signal quality) were downloaded every 2 sec and analysed only when the signal had no alarm messages (low IQ signal, low perfusion, sensor off, ambient light). / Results. Observational studies: The dataset to develop the reference range charts included 61,650 data points from 468 infants. Infants had a mean (range) gestational age of 38 (25-42) weeks and birthweight 2970 (625-5135) g. For all 468 infants at one minute the 3rd, 10th, 50th, 90th and 97th centiles were 29%, 39%, 66%, 87% and 92%; at two minutes 34%, 46%, 73%, 91% and 95% and at five minutes 59%, 73%, 89%, 97% and 98%. It took a median of 7.9 (IQR 5.0 to 10) minutes to reach a SpO2 > 90%. SpO2 of preterm infants rose more slowly than that of term infants. At one min the median (IQR) HR was 82 (66 to 138) bpm rising at two min and five min to 151 (112 to 169) bpm and 166 (148 to 176) bpm respectively. In preterm infants, the SpO2 and HR rose more slowly than term infants. / Randomised trial: Forty nine infants were randomly allocated to the T-piece and 50 to the SIB. Ten infants did not receive PPV, 4 (8%) in the T-piece group and 6 (12%) in the SIB group and were not included in the analysis. Forty-one infants received PPV with a T-piece and 39 with a SIB. At 5 minutes after birth there was no significant difference between the mean (SD) SpO2 in the T-piece and SIB groups [50 (31)% vs. 53 (25)%, (p=0.73)]. More T-piece infants received oxygen during DR resuscitation (100% vs. 90%, p=0.04). There was no significant difference between the groups in the use of continuous positive airway pressure (CPAP); endotracheal intubation or administration of surfactant in the DR. Fewer of the T-piece group who left the DR on CPAP were intubated in the first 24 hrs after birth. (7% vs. 23%, p=0.05). / Conclusion. The centile charts developed in this thesis provide a reference range for SpO2 and HR in the first 10 minutes after birth for preterm and term infants. In the randomised trial there was no significant difference in SpO2 at five minutes after birth in extremely preterm infants given PPV with a T-piece or a SIB.

Pulse Width Modulation (PWM) has been used extensively for motor control, DC-AC converters, DC-DC converters and in audio applications. The conventional method of generating a pulse width modulated signal involves generating an accurate sawtooth or triangle wave using analog circuits. In CMOS, being analog circuit intensive puts extra constraints on the fabrication process used for manufacture, thus requiring a higher cost than digital CMOS. Delta-Sigma Modulation on the other hand is analog intensive, but requires only quality capacitor matching, which can be obtained with current digital CMOS processes. This thesis describes a method to generate a digital PWM with pulse dithering using a Delta-Sigma modulator. The Delta-Sigma modulator provides accuracy and allows the circuit to be implemented in a digital CMOS process, while the pulse dithering in the PWM spreads out harmonic noise generated from the PWM fundamental frequency. / Graduation date: 1998

Donald Winston Tufts. / "July 28, 1960." Issued also as a thesis, M.I.T. Dept. of Electrical Engineering, May 25, 1960. / Bibliography: p.48. / Army Signal Corps Contract DA36-039-sc-78108. Dept. of the Army Task 3-99-20-001 and Project 3-99-00-000.

[by] E.R. Kretzmer. / "This report is identical with a thesis of the same title submitted by the author in partial fulfillment of the requirements for the degree of Doctor of Science in Electrical Engineering at the Massachusetts Institute of Technology." / Bibliography: p. 238-239. / Army Signal Corps Contract No. W-36-039 sc-32037. Dept. of the Army Project No. 3-99-10-022.

W.C. Galloway. / "February 4, 1948." / Bibliography: p. 16. / Army Signal Corps Contract No. W-36-039 sc-32037.

E.R. Kretzmer. / "Reprinted from the Proceedings of the Institute of Radio Engineers ; v.35, no.11" / Includes bibliographical references. / Army Signal Corps Contract No. W-36-039 sc-32037.

Thesis (M.S.)--University of Missouri-Columbia, 2006. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (February 26, 2007) Includes bibliographical references.

Nonlinear optical microscopy (NLOM) utilizing femtosecond laser pulses is well suited for imaging living tissues. This work reports on the design and development of an optical fiber based multispectral NLOM developed around a laser generating broadband sub-10-fs pulses. An all-mirror dispersion-compensation setup is used to correct for quadratic and cubic phase distortions induced within the NLOM. Mouse tail tendon was used to characterize sub-10-fs pulses by interferometric autocorrelation. This is an effective method for characterizing dispersion from the optical system, immersion medium, and wet biological sample. The generation of very short autocorrelations demonstrates the ability to compensate for phase distortions within the imaging system and efficient second-harmonic upconversion of the ultrashort pulse spectrum within collagen. Reconstruction of ultrashort pulses at the focal plane of the objective allows the excitation of multiple fluorescent probes simultaneously. Multiple fluorescent probe excitation and spectral discrimination is demonstrated using mixtures of fluorescent dye solutions and an in-vitro angiogenesis model containing human umbilical vein endothelial cells (HUVEC’s) expressing multiple fluorescent proteins. Sub-10-fs pulses can be propagated through polarization-maintaining single mode fiber (PMF) for use in NLOM. We demonstrate delivery of near transform-limited, 1 nJ pulses from a Ti:Al2O3 oscillator via PMF to the NLOM focal plane while maintaining 120 nm of bandwidth. Negative group delay dispersion (GDD) introduced to pre-compensate normal dispersion of the optical fiber and microscope optics ensured linear pulse propagation through the PMF. Nonlinear excitation of multiple fluorophores simultaneously and polarization sensitive NLOM imaging using second harmonic generation in collagen was demonstrated using PMF delivered pulses. Two-photon excited fluorescence spectra and second harmonic images taken with and without the fiber indicates that the fiber based system is capable of generating optical signals that are within a factor of two to three of our traditional NLOM.