Spelling suggestions: "subject:"insitu microscopy"" "subject:"in.situ microscopy""
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Online Image Analysis of Jurkat T Cells using in situ MicroscopyJoensuu, Jenny January 2015 (has links)
Cell cultivation in bioreactors would benefit from developed monitoring systems with online real-time imaging to evaluate cell culture conditions and processes. This opportunity can be provided with the newly developed in situ Microscope also called ISM. The ISM probe is mounted into the wall of a bioreactor and consists of a measurement zone with an illuminating light source to obtain real-time images of moving cells in suspension. The instrument is linked to advanced imaging analysis software which can be specifically adapted for the objects in study. The aim of this project is to analyze the T lymphocyte cell line Jurkat T cells using the ISM equipment and identify specific features of the cells that can be obtained. The results show that the equipment and linked software are suitable for monitoring cell density, cell size distribution and cell surface analysis of the Jurkat cells during cultivation. The ISM could also detect induced changes in cell size caused by osmotic shifts and the course of an infection occurring in the cell suspension using a developed software for online real-time monitoring.
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FABRICATION, PLASTICITY AND THERMAL STABILITY OF NANOTWINNED AL ALLOYSQiang Li (7041092) 12 October 2021 (has links)
<p>Applications of Aluminum (Al) alloys in harsh
environments involving high stress and high temperatures are often hindered
because of their inherently low strength and poor performance at high
temperatures. The strongest commercial Al alloys reported up to date have a
maximum strength less than 700 MPa. Although ultrafine grained Al alloys
prepared by severe plastic deformation have higher strength, they encounter
grain growth at moderate temperatures. </p>
<p>This thesis focuses on adopting transition metal
solutes and non-equilibrium approach to fabricate high-strength, thermally
stable nanotwinned Al alloys. To understand the underlying deformation
mechanisms of nanotwinned Al alloys, <i>in-situ</i>
micromechanical tests, high resolution and analytical transmission microscopy
and atomistic simulations were used. Our studies show that nanotwinned
supersaturated Al-Fe alloys have a maximum hardness and flow stress of ~ 5.5
GPa and 1.6 GPa, respectively. The apparent directionality of the vertical
incoherent twin boundaries renders plastic anisotropy and compression-tension
asymmetry in the nanotwinned Al-Fe alloys, revealed by systematic <i>in-situ</i> tensile and compressive
micromechanical experiments conducted from both in-plane and out-of-plane
directions. Moreover, the nanotwinned Al-Fe alloys experience no apparent
softening when tested at 200 °C. When selectively incorporating with one
additional solute as stabilizer, the ternary nanotwinned Al alloys can preserve
an exceptionally high flow stress, exceeding 2 GPa, prior to precipitous
softening at an annealing temperature of > 400 °C. The thesis offers a
new perspective to the design of future strong, deformable and thermally stable
nanostructured Al alloys. </p>
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In-Situ Environmental TEM Studies of Electro- and Photo-Electrochemical Systems for Water SplittingRonge, Emanuel 18 December 2020 (has links)
No description available.
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