In functional magnetic resonance imaging (fMRI) studies, inter-subject anatomical variability of the human brain has been a major challenge in finding reliable functional/anatomical correspondences. Assessment of brain-behavior relations involves a series of geometrical/statistical operations on brain images to minimize such inter-subject variability, so that group maps of brain activity relative to brain anatomy can be developed. Various methods of image registration, segmentation, and analysis have been proposed for mapping functional activity on to anatomical atlases of the brain. The two most common techniques that have been widely accepted and used by neuroimaging scientists are volume-based (VB) analysis using group registration methods and region-of-interest (ROI)-based methods using automated segmentation algorithms or macro/microanatomical probabilistic atlases for labeling. Nevertheless, the analysis results based on these techniques are significantly affected by the accuracy of the selected segmentation and/or registration methods. Furthermore, conventional fMRI data analysis techniques (VB, and ROI-based methods) mainly rely on the assumption that brain processes are common and universal among individual humans; however, besides anatomical differences, there also exist cognitive and behavioral variability among individuals due to differential engagement of brain networks even when performing an identical cognitive task. In this thesis, I have assessed the impact of anatomy-based alignment techniques (VB, and ROI-based methods) on sensitivity of fMRI data group analysis. I evaluated the effect of the type of inter-subject registration used and related factors on sensitivity of group-level fMRI data analysis. Furthermore, I have also assessed the goodness of fit of probabilistic maps by proposing an evidence-based framework for evaluation of probabilistic maps. As a test model, I have selected the human auditory cortex. Auditory cortex is an interesting yet challenging case with substantial inter-individual functional/anatomical variability. For the sake of ROI-based method of analysis, I have proposed a novel approach for automatic segmentation of Heschl's gyrus, which is the landmark for primary auditory cortex. Finally, in order to assess the impact of inter-subject variability in anatomy on functional organization, I analyze data from an fMRI study, which demonstrates that the degree to which anatomical registration compensates for functional variability depends on the brain region activated. / Thesis (Ph.D, Computing) -- Queen's University, 2010-04-29 07:07:55.77

Dissertation submitted in partial compliance with the requirements for the Masters Degree in Technology: Chiropractic, Durban University of Technology, 2010. / The aim of this study was to determine the inter-examiner reliability of motion palpation to detect joint dysfunction in hindfoot and midfoot joints of asymptomatic feet and feet with chronic ankle instability syndrome. The rationale for this study was that motion palpation is a commonly used assessment tool that is used by the chiropractic profession to detect the need for manipulation of the spine and extremities. Also until the reliability of motion palpation is known, other studies using motion palpation as an assessment tool to detect the need for manipulation in the hindfoot and midfoot are questionable. The study was conducted at Durban University of Technology (DUT). Patients that responded to the adverts were then screened via telephonic interview. The researcher performed a case history, physical examination and a foot and ankle regional examination on each patient. Three masters chiropractic students then independently assessed both the symptomatic and asymptomatic feet of each patient and recorded their results. The data was then statistically analysed using SPSS version 15. It was found that the inter-examiner reliability of motion palpation for detecting restrictions in feet with chronic ankle instability syndrome was fair and for detecting instability, there was moderate reliability. In the asymptomatic group the examiners showed to have poor reliability in detecting restrictions and moderate reliability in detecting instability. Inter-examiner reliability was better in the symptomatic group and in this group examiners had more agreement on detecting instability as opposed to restrictions. This study has showed that inter-examiner reliability ranged from poor to moderate in the symptomatic and asymptomatic group with the reliability ranging from poor to moderate. Therefore, one can conclude that motion palpation can be used as an assessment tool to detect joint dysfunction in hindfoot and midfoot joints. However, further studies are warranted to address other subjective and objective measurements such as tenderness and range of motion together with motion palpation.

Dissertation submitted in partial compliance with the requirements for the Masters Degree in Technology: Chiropractic, Durban University of Technology, 2011. / Background: Myofascial pain is a disorder, characterized by the presence of trigger points (MTrP). It is recognised by unique features which include a tender point in a taut band of muscle, a local twitch response (LTR), a characteristic referred pain pattern, and the reproduction of the patient’s usual pain upon examination. A debate exists as to the precise diagnostic criteria used in identifying trigger points. This has hampered the standardized assessment and treatment of Myofascial Pain Syndrome and has led to contradictory findings being reported by various authors due to the lack of a reliable diagnostic tool. Objectives: The first objective was to determine the inter-examiner reliability of palpation of MTrPs in the trapezius and gluteus medius muscles. The second objective was to determine whether training and standardization in palpation techniques would improve inter-examiner reliability of palpation of MTrPs. Methods: This study was designed as a quantitative pre and post intervention interexaminer reliability study. Three examiners (one qualified Chiropractor, one senior chiropractic intern from the CDC and the researcher) were used to examine sixty patients (thirty symptomatic and thirty asymptomatic) for MTrPs. This study was conducted in two phases. During the myofascial examination of patients examiners were required to determine whether a MTrP was present or absent, differentiate whether the MTrP was active or latent and determine the presence or absence of the five characteristics of MTrP (tender point in a taut band of muscle, a local twitch response (LTR), a pain characteristic referred pain pattern, the reproduction of the patient’s usual pain and a jump sign) however, in phase one the researchers were blinded to the characteristics being investigated. Subsequent to phase one, examiners had to attend two, one hour discussion sessions to reduce individual variation in the application of palpation techniques. Results: Inter-examiner reliability was assessed using Fleiss Kappa statistic, percentage agreement and confidence intervals. The results show that three examiners are able to attain acceptable agreement in the palpation of MTrPs, since the features (described above) were shown to improve considerably in phase two after the training session in which standardization of techniques was emphasized. Conclusion: This study provides preliminary evidence that MTrP palpation is reliable and therefore, useful diagnostic tool in the identification of MTrPs and the diagnosis of Myofascial Pain Syndrome.

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Network relationships play a significant role in the provision of servitized offerings. To date, little empirical research has been conducted to investigate the link between servitization and inter-organisational relationships. The objective of this doctoral thesis is to explore the implications of servitization on a manufacturer’s network. Particular emphasis is placed on the impact on the network structure and relationship attributes. An exploratory in-depth case study was conducted within the truck manufacturing industry using a multi-organisational perspective. An abductive research approach was adopted which was underlined by pragmatism. As part of this approach, 43 interviews were conducted in a total of 11 companies. The findings of the study suggest that managers need to be aware of the different customer needs, related offerings and resultant implications on the network structure and relationships. To this end, the findings show that as the offerings move towards advanced servitized offerings the network becomes more complex in terms of its structure and relationships. The research contributes to the literature by providing a more nuanced description of what actually occurs in a network when a manufacturer provides servitized offerings in conjunction with other product-based offerings. In particular, it identifies the relationship attributes that need to be managed in order to drive the right behaviour for the provision of each of these offerings. Moreover, it is the first known study to uncover triadic as well as tetradic network structures in a servitization context. Equally important, it provides a framework that captures the interplay between the different offerings and the resultant network structure and relationship attributes. In all of these capacities, this research is one of the first known studies to uncover some of the complexities surrounding the way in which inter-organisational relationships are enacted in a servitization context.

In the early 1990s Graham Nuthall and Adrianne Alton-Lee developed a model of the learning and remembering process which has profound implications for teaching and learning at all levels. Using their model they were able to predict what selected primary school students would and would not learn and remember from the teaching of a series of Science and Social Studies units and to do so with an accuracy of between 80 to 85 per cent. The Nuthall model states that for a student to learn and remember a new fact or concept he or she needs three to four learning opportunities with the complete set of information needed to learn the new fact or concept, and a gap of no more than two days between any pair of those two learning opportunities. It had always been Graham Nuthall's intention to test the model he developed with Adrienne Alton-Lee in a series of experiments. Tragically, Professor Graham Nuthall died before this was possible. The ten experiments in this thesis put the Nuthall model to the test.

Pluripotent stem cells (PSCs) can self-renew indefinitely in culture while maintaining their capacity to differentiate into any cell type of an organism, thus offering novel sources for drug screening, in vitro disease modelling, and cell replacement therapies. However, due to their sensitive nature, many PSC lines are still cultured using undefined components such as serum or serum-derived components, on either feeder cells or complex protein mixes such as Matrigel or gelatine. In order to fully realize the potential of these cells we need controlled, completely defined and xeno-free culturing conditions that maintain growth and survival of homogenous, non-differentiated colonies. This thesis focuses on the in vitro maintenance of both mouse and human PSCs, analysing the media and substrate requirements of these cells and linking them to the intracellular signalling pathways involved in the maintenance of pluripotency and self-renewal. Benchmarking of commercially available culture methods for PSCs has been performed, evaluating their capacity to maintain pluripotency and growth of undifferentiated PSCs over several passages and reporting new characteristics, like the tendency of mouse PSCs to grow as floating spheres in 2i medium, a novel media formulation that uses two inhibitors to hinder differentiation capacity and subsequently induce pure, undifferentiated cultures. The major finding in this thesis is the identification of Inter-α-Inhibitor (IαI) as a protein able to activate the previously described signal-transduction pathway Yes/YAP/TEAD in mouse PSCs and to induce transcription of the well-known stem cell transcription factors Nanog and Oct3/4. IαI is a serum protein found in high concentration in human serum that had been traditionally described as an extracellular matrix remodelling protein. For the first time, we describe IαI to have signalling capacity on PSCs. Moreover, IαI is demonstrated to induce attachment, growth and long-term survival of undifferentiated mouse and human PSCs when added to serum-free, chemically defined media. IαI is the first molecule described to date to induce attachment of human PSCs on uncoated, standard tissue-culture treated plastic, just by supplementation as a soluble molecule at the seeding step. Following this discovery, we evaluate a novel culture method using the completely defined, serum-free E8 medium supplemented with IαI (E8:IαI) for long-term propagation of four different human PSC lines and discover that IαI can indeed support long-term culture with maintained pluripotency, differentiation capacity, growth rate and genetic stability. Moreover, in contrast to the control culture method using a commercially available surface coating, IαI supplementation can support single cell passaging of human PSCs, and adapt feeder-dependent cultured human PSCs to E8:IαI with high efficiency. A mouse PSC line is also grown for over 20 passages in IαI with retained pluripotency, differentiation capacity and genetic stability. IαI is inexpensive to produce and derived from human plasma, and could therefore be produced in compliance with Good Manufacturing Practices. Ultimately, our group aims to develop and test large-scale, completely defined, xeno-free culturing methods for PSCs, suitable for pharmacological and medical applications.

No description available.

© Cranfield University 2013. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright owner. / Network relationships play a significant role in the provision of servitized offerings. To date, little empirical research has been conducted to investigate the link between servitization and inter-organisational relationships. The objective of this doctoral thesis is to explore the implications of servitization on a manufacturer’s network. Particular emphasis is placed on the impact on the network structure and relationship attributes. An exploratory in-depth case study was conducted within the truck manufacturing industry using a multi-organisational perspective. An abductive research approach was adopted which was underlined by pragmatism. As part of this approach, 43 interviews were conducted in a total of 11 companies. The findings of the study suggest that managers need to be aware of the different customer needs, related offerings and resultant implications on the network structure and relationships. To this end, the findings show that as the offerings move towards advanced servitized offerings the network becomes more complex in terms of its structure and relationships. The research contributes to the literature by providing a more nuanced description of what actually occurs in a network when a manufacturer provides servitized offerings in conjunction with other product-based offerings. In particular, it identifies the relationship attributes that need to be managed in order to drive the right behaviour for the provision of each of these offerings. Moreover, it is the first known study to uncover triadic as well as tetradic network structures in a servitization context. Equally important, it provides a framework that captures the interplay between the different offerings and the resultant network structure and relationship attributes. In all of these capacities, this research is one of the first known studies to uncover some of the complexities surrounding the way in which inter-organisational relationships are enacted in a servitization context.

La thèse part d’un premier constat : le manque de réflexion sur les questions d’identité, d’altérité et d’intégration dans des sociétés occidentales pourtant de plus en plus hétérogènes. Le traitement médiatique québécois des accommodements raisonnables au milieu des années 2000 a ainsi rappelé la difficulté d'habiter plusieurs univers culturels à la fois. Comme si revendiquer une appartenance, c'était renoncer à toutes les autres. Comme si appartenir à une communauté religieuse, c'était y être enfermé. Notre thèse défend l’idée que les divergences identitaires et culturelles sont inhérentes à toute démocratie. Elles ne lui font donc pas obstacle, sauf quand des situations comme le traitement médiatique québécois des accommodements raisonnables tendent à accentuer ces divergences au point de rendre impossible toute communication entre cadres référentiels. Pour observer ce traitement médiatique, nous nous sommes limitée à une altérité en particulier, celle des minorités arabes et/ou musulmanes. L’observation privilégiée était par ailleurs très succincte #trois articles traductifs, trois articles non-traductifs et trois articles intraductifs tirés du journal Le Devoir#. Nous avons mobilisé une théorie de la traduction inter-référentielle pour mieux saisir les mécanismes discursifs qui ont pour vocation de montrer comment la représentation de l’Autre trahit en même temps la représentation de soi, celle de son cadre de référence ainsi que le contexte social dans lequel s’inscrivent ces représentations respectives. Nous avons également eu recours au concept de traduction citoyenne #Basalamah# qui part du principe qu’un cadre référentiel ne doit jamais être considéré comme autosuffisant. Le traducteur citoyen invite en ce sens le lecteur à une éventuelle remise en question, au repositionnement de soi vis-à-vis de l’Autre ainsi qu’au rapprochement mutuel. Or, que nous montre l’analyse des neuf articles tirés du journal Le Devoir? Elle nous montre d’abord que se situer dans une posture non traductive, c’est oublier sa dette envers l’Autre. Elle nous montre ensuite que se situer dans une posture intraductive, c’est courir derrière de vaines illusions #l’illusion d’être objectif, l’illusion d’être non-engagé ou parfois encore l’illusion de ne pas reproduire de vieilles hiérarchies#. Enfin, elle nous montre que même la posture traductive peut décourager le dialogue quand elle s’engage dans une voie trop moralisante.