The aim of this thesis is to offer an application of the concept of pervasivity on the field of live art. The first chapter introduces the concept as it was defined in the field of game studies, mainly by scholars of Tampere school. To fill this aim it makes use of terminology coming chiefly from social sciences. The second chapter describes the relationship between game and theatre including their common as well as dissimilar features and then adapts the pervasivity concept for the theatre field. The third chapter brings possible applications of the concept by presenting three examples of a piece's confrontation of everydayness: through accentation, fabrication and construction. The conclusion sums up possible pervasive moments in the examined pieces of art.

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5 ABSTRACT The mitogen-activated protein kinase (MAPK) cascade is an essential member of the cell defense system against stressors. The capability and efficiency of the cell reactions to different stress signals depend on signal transduction pathway, where signals from MAPK kinase kinase (MAP3K) are transferred through phosphorylation to downstream MAPK kinase (MAP2K) and finally to MAPK. Apoptosis signal-regulating kinase 1 (ASK1) is a member of a MAP3K family and its activation and inhibition has a significant participation in a regulation of cell response to stress stimuli. The regulation of ASK1 has a strong influence in pathogenesis of several diseases, the excessive activation of human ASK1 or failure in the control of its function are associated with cardiovascular diseases, neurodegenerative disorders, inflammatory diseases, infectious diseases, tumorigenesis, asthma, diabetes and ageing. The activity of ASK1 is regulated by its interaction with several proteins, the attention is focused on two physiological inhibitors, mammalian thioredoxin (TRX) and the 14-3-3 protein. ASK1 in its inactive form is inhibited by bonds formation with TRX and 14-3-3, however the explicit mechanism of this interaction is unclear due to the absence of structural data. This work is a part of an extensive research about...

MAP kinase signaling cascade plays an important role in the cellular response to various stress stimuli from the external environment. This signaling cascade is divided into three levels: MAP kinase kinase kinases (MAP3K) phosphorylate and thus activate MAP kinase kinases (MAP2K) and those subsequently phosphorylate and thus activate MAP kinase (MAPK) pathway, which regulates many cellular functions such as apoptosis, cell differentiation and morphogenesis. One of the important MAP3K is protein kinase ASK1 (Apoptosis signal-regulating kinase 1), which is an important regulator of cellular immune and stress responses. Given that the increased activity of ASK1 is related to the development of serious diseases such as cancer, cardiovascular and neurodegenerative diseases, ASK1 is an interesting target in the pharmacy in the development of new drugs. Human ASK1 consists of 1374 amino acids and is divided into three domains: a central Ser/Thr catalytic domain and two coiled-coil domains, of which the first is located at the N- and the second at the C-terminus of the molecule of this protein kinase. ASK1 is regulated by its binding partners, which include a small cellular redox protein thioredoxin (Trx-1), which binds to the N-terminal part of ASK1. Trx-1 is a potent antioxidant and so it protects cells...

Temperature dependences of nuclear magnetic resonance spectra of isotopes 207 Pb, 137 Ba, and 93 Nb in polycrystalline samples of PbxBa1-x(FeNb)0.5O3, with x = 0 (BFN), 0.5 (PBFN), 1 (PFN) and in Pb(FeNb)0.5O3 single crystal (PFN SC) were acquired. Measured nuclear magnetic resonance spectra are analyzed in this work. The temperature dependence of the spectra show strong broadening with decreasing temperature. Part of the temperature dependences of nuclear magnetic resonance spectra did not comply with expected characteristics. Possible hypotheses for interpretation of such behavior are discussed in the text. Probable arrangement of Fe and Nb cations in PFN and BFN phases is deduced and, based on these differences, the contrast in Néel temperatures of these phases is explained.

Interactive collaborative enviroments are solved by many companies and research teams. This thesis is focused on design and development of own system for gesture recognition based on Microsoft Kinect controller. The system is focused on collaboration of multiple users. Therefore, proposed solution can distinguish interactions of different users. The result allows to create an interactive area from a common wall using a computer, projector and Kinect controller.

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Veronika Nováková Supervisor: PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: Food interactions of antibiotics Diploma thesis deals with clinically significant interactions between antibiotics and food. It defines basic concepts such as antibiotics, food, drug interactions and also drug-food interactions. The main part of the work deals with the characteristics of individual groups of antibiotics, describes their mechanism of action, including the antimicrobial spectrum, the main side effects and deals with the already mentioned interactions with food and beverages. St. John's wort, grapefruit, alcohol, or polyvalent ions in particular play an important role in these interactions, which can have a significant effect on the course of antibiotic therapy. The next part of this work deals with the effect of probiotics on the natural intestinal microflora, which is disrupted by antibiotics. The last part of the thesis deals with the issue of antibiotic resistance and the principles of correct and appropriate use of antibiotics, which have an impact not only on minimizing the emergence and development of bacterial resistance, but also help to prevent interactions, including drug-food...

Abiotic and biotic factors directly or indirectly influence physiology, behaviour, ecology and the distribution of organisms. This thesis provides unique results in terms of the ability of crayfish to cope with a series of these abiotic and biotic factors. Successful overwintering is key factor facilitating establishment and spread of non-indigenous species. We provide experimental evidence of substantial resistance of three out of four warm water crayfish species to the low water temperatures tested in our study. To sum up, red swamp crayfish together with yabby demonstrated high survival in experimental conditions and exhibited feeding activity even during the coldest part of the experiment. Marbled crayfish suffered high mortality, but a single specimen of survived. Redclaw is expected not to pose a risk for the European temperate zone in the near future. Drought is an important factor for freshwater organisms determining their success in a given ecosystem. One of the strategies how to survive drought are an elevated desiccation capacity and burrowing ability. We evaluated the ability of three European native and five non-native crayfish to survive and burrow in a humid sandy-clayey substrate under a simulated one-week drought. Survival of non-native species was substantially higher, being absolute in the red swamp crayfish and yabby. Red swamp crayfish and marbled crayfish constructed bigger and deeper burrows than yabby and spiny-cheek crayfish. Three native species (noble crayfish, narrow-clawed crayfish and stone crayfish) suffered extensive mortalities. The native species and signal crayfish exhibited no ability to burrow under drought conditions. Marbled crayfish are a successful invader, capable of colonization new habitats. It is also established in the wild of Ukraine and pet-traded in Eastern Europe. If released, it might pose a risk to populations of native crayfish that also inhabit saline ecosystems of the Black, Azov and Caspian Sea. We conducted the first study on salinity tolerance in marbled crayfish. In summary, marbled crayfish are probably unable to invade saline ecosystems due to its low survival, reduced growth and inhibited reproduction. Nevertheless, it showed an ability to withstand the highest salinity for an extended period of time, opening a possibility for its spread to other freshwaters via this corridor. Also, a short generation time might lead to local adaptations to saline environments in the future. Yabby demonstrated an ability to withstand simulated low winter temperatures, opening the question of its potential establishment in the region of Central Europe. To successfully do so, yabby would have to deal with present strains (A, B, and E) of Aphanomyces astaci, a causative agent of crayfish plague, a devastating disease to all crayfish not originating from North America. Our comparative study revealed the ability to survive (strain A) or delay the effects (strains B and E) of crayfish plague compared to native noble crayfish. Our result, highlights a high potential for its establishment in temperate Europe. As a prominent invader, it may cause negative impacts on entire ecosystems and contribute to the spread of crayfish plague in Europe. Nowadays, organisms face multiple global drivers influencing the strength of links in food webs. Altogether, we found that predator identity, prey density, and temperature all affect trophic and non-trophic interaction strengths. Effects of prey density were always independent of temperature and predator identity in trophic as well as non-trophic interaction strength. On the other hand, the magnitude of responses in trophic as well as non-trophic interaction strengths varied strongly across predator pairs and temperatures. Based on the current evidence, we could not find strong support for a general mechanism underlying the dependence of interaction strengths on species traits and environmental conditions. Therefore, other studies are need it.

Interaction of drugs used in gynecology and urology and their management Author: Kupka Vojtěch Tutor: PharmDr. Martin Doseděl Ph.D., Department of Social and Clinical Pharmacy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové Introduction: Drug interactions represent a serious health problem and they cause adverse drug effects and toxicity of drugs. Objectives: The goal of the thesis was to create an overview of possible drug interactions with drugs used in gynecological and urological practice. Methodology: From a theroretical introduction was selected registred drugs to 1 st January 2015 by State Institute for Drug Controle. Information about drug interactions were collected and collated from databases Vademecum Infopharm and Micromedex. From database Micromedex, the drug interactions were selected with quality of documentation "excellent" or "good". Pubmed database (www.pubmed.gov) with entering key words "basic drug", "drug interaction" and "interactive drug" was applied to create a current information. To complement the information was also gathered from magazines Practical Pharmacy and Clinical Pharmacology and Pharmacy. In case of not finding relevant information was drawn from the summary information of medicine (SPC). Results: The results were categorized into a research...