No / Greedy and contention-based forwarding schemes were proposed for mobile ad hoc networks (MANETs) to perform data routing hop-by-hop, without prior discovery of the end-to-end route to the destination. Accordingly, the neighboring node that satisfies specific criteria is selected as the next forwarder of the packet. Both schemes require the nodes participating in the selection process to be within the area that confronts the location of the destination. Therefore, the lifetime of links for such schemes is not only dependent on the transmission range, but also on the location parameters (position, speed and direction) of the sending node and the neighboring node as well as the destination. In this paper, we propose a new link lifetime prediction method for greedy and contention-based routing which can also be utilized as a new stability metric. The evaluation of the proposed method is conducted by the use of stability-based greedy routing algorithm, which selects the next hop node having the highest link stability.

The interior of the cell is a densely crowded and complex arena, full of a vast and diverse array of molecules and macromolecules. A fundamental understanding of cellular physiology will depend not only upon a reductionist analysis of the chemistry, structure, and function of individual components and subsystems, but also on a sagacious exegesis of the dynamic and emergent properties that characterize the higher-level system of living cells. Here, we present work on two aspects of the supramolecular organization of the cell: the controlled assembly of the mitotic spindle during cell division and the regulation of cellular metabolism through the formation of multienzyme complexes. During division, the cell undergoes a profound morphological and molecular reorganization that includes the creation of the mitotic spindle, a process that must be highly controlled in order to ensure that accurate segregation of hereditary material. Chapter 2 describes results that implicate the kinase, Zeste-white3/Shaggy (Zw3/Sgg), as having a role in regulating spindle morphology. The congregation of metabolic enzymes into macromolecular complexes is a key feature of cellular physiology. Given the apparent pervasiveness of these assemblies, it seems likely that some of the mechanisms involved in their organization and regulation might be conserved across a range of biosynthetic pathways in diverse organisms. The Winkel laboratory makes use of the flavonoid biosynthetic pathway in Arabidopsis as an experimental model for studying the architecture, dynamics, and functional roles of metabolic complexes. Over the past several years, we have accumulated substantive and compelling evidence indicating that a number of these enzymes directly interact, perhaps as part of a dynamic globular complex involving multiple points of contact between proteins. Chapter 3 describes the functional analysis of a predicted flavonol synthase gene family in Arabidopsis. The first evidence for the interaction of flavonoid enzymes in living cells, using fluorescent lifetime imaging microscopy fluorescent resonance energy transfer analysis (FLIM-FRET), is presented in Chapter 4. / Ph. D.

Wireless ad-hoc networks are easily deployed, untethered to infrastructure, and have virtually an unlimited number of applications. However, this flexibility comes at the cost of finite and often unreplenishable power supplies. Once a node has consumed all of its power, it can no longer receive, transmit, gather information, or otherwise participate in the network. Therefore, reducing the amount of energy necessary for node communication has been an area of intense research. Previous work has investigated the use of directional antennas as a method to reduce inter-node power requirements. However, most proposed methods ignore inter-session interference, propose heuristic solution methods, and ignore the use of directional antennas for signal reception. We develop a flexible mixed-integer linear program (MILP) designed to optimize max-min multicast path lifetime for directional antenna equipped networks in the presence of interference. The MILP is utilized to perform a comparison directional antenna use for signal transmission and reception. Results indicate that directional reception is slightly superior to transmission for the defined max-min lifetime metric, and vastly superior when considering cumulative power use. We further analyze the performance of interference-ignorant link-based heuristics designed for both directional transmission and directional reception as they perform in our more realistic model. Our results show that interference-ignorant methods cannot find feasible solutions unless all nodes are equipped with high gain, high efficiency directional antennas. Even in these cases, directional reception outperforms directional transmission. Because of the superiority of directional reception, we focus our attention on this method. A heterogeneity study is performed, and two heuristic methods for approximating the MILP optima are developed. We find that even under heterogeneous conditions, directional reception can increase network lifetime. Finally, a genetic algorithm (GA) and semi-distributed heuristic method are developed as alternatives to the MILP. Results show that the GA often can find solutions with lifetimes 85% as long as the optimal. Our semi-distributed heuristic, designed to be even more computationally simple than the GA, and to serve as a basis for a distributed protocol, is almost as effective as the GA as approximating optimal solutions. We conclude that directional reception is the superior method of antenna use for extending max-min multicast tree lifetime, that it works well in heterogeneous conditions, and lends itself well to heuristic design. / Ph. D.

This dissertation examines the carrier transport properties, diffusion length, effective carrier lifetime, and resistivity in two wide bandgap materials, GaN and diamond. A combination of two methods was used to obtain these transport properties. The two were optical beam induced current (OBIC) and electron beam induced current (EBIC) time of flight transient measurements. These techniques consist of measuring the current response to the drift and diffusion of generated electron-hole pair carriers created by a short-duration pulse of radiation. Under OBIC, a short duration pulsed optical source, with an electron beam excitation pulse time much less than the transit time of the material, was used to generate excess carriers within the absorption depth of the material. The second method of excitation, EBIC involved the use of a modified SEM with a photoemission source (L-EBIC) and a high speed pulsed thermionic electron source (T-EBIC) to generate an electron beam. This electron beam was used to create a large number of electron-hole pairs at various penetration depths within the materials. Measurements on GaN found the diffusion length was 7.84 µm with the L-EBIC and 7.78 µm with the T-EBIC. After annealing at 900°C for 30 min. the GaN diffusion length increased to 9.89 µm (L-EBIC). The dark resistivity was 1.79 x 10¹⁰Ω-cm, and the carrier lifetimes were 1.7 µs with L-EBIC and 3.36 & 3.9 ns with OBIC. The author believed that the L-EBIC result was a good representation of the carrier lifetime within the material, while the shorter OBIC results were due to the combine high surface and interface recombination processes. The diamond dark resistivity was found to be 6.14 x 10¹¹Ω-cm and the diffusion lengths were 94.1 µm and 97 µm from the L-EBIC and T-EBIC respectively. All measurements were within 10 % spread. The real value of this contribution lies in determining the diffusion lengths in GaN and diamond by the EBIC techniques, measuring the effective surface\interface and thin film carrier lifetime of GaN utilizing a combination of OBIC and L-EBIC techniques, and evaluating the dark resistivity in GaN and diamond materials. These measurements can lead to a better understanding and exploitation of the electrophysical behavior of these materials. / Ph. D.

Product reliability is an important characteristic for all manufacturers, engineers and consumers. Industrial statisticians have been planning experiments for years to improve product quality and reliability. However, rarely do experts in the field of reliability have expertise in design of experiments (DOE) and the implications that experimental protocol have on data analysis. Additionally, statisticians who focus on DOE rarely work with reliability data. As a result, analysis methods for lifetime data for experimental designs that are more complex than a completely randomized design are extremely limited. This dissertation provides two new analysis methods for reliability data from life tests. We focus on data from a sub-sampling experimental design. The new analysis methods are illustrated on a popular reliability data set, which contains sub-sampling. Monte Carlo simulation studies evaluate the capabilities of the new modeling methods. Additionally, Monte Carlo simulation studies highlight the principles of experimental design in a reliability context. The dissertation provides multiple methods for statistical inference for the new analysis methods. Finally, implications for the reliability field are discussed, especially in future applications of the new analysis methods. / Ph. D.

Understanding the lifetime behavior of their products is crucial to the success of any company in the manufacturing and engineering industries. Statistical methods for lifetime data are a key component to achieving this level of understanding. Sometimes a statistical procedure must be updated to be adequate for modeling specific data as is discussed in Chapter 2. However, there are cases in which the methods used in industrial standards are themselves inadequate. This is distressing as more appropriate statistical methods are available but remain unused. The research in Chapter 4 deals with such a situation. The research in Chapter 3 serves as a combination of both scenarios and represents how both statisticians and engineers from the industry can join together to yield beautiful results. After introducing basic concepts and notation in Chapter 1, Chapter 2 focuses on lifetime prediction for a product consisting of multiple components. During the production period, some components may be upgraded or replaced, resulting in a new ``generation" of component. Incorporating this information into a competing risks model can greatly improve the accuracy of lifetime prediction. A generalized competing risks model is proposed and simulation is used to assess its performance. In Chapter 3, optimal and compromise test plans are proposed for constant amplitude fatigue testing. These test plans are based on a nonlinear physical model from the fatigue literature that is able to better capture the nonlinear behavior of fatigue life and account for effects from the testing environment. Sensitivity to the design parameters and modeling assumptions are investigated and suggestions for planning strategies are proposed. Chapter 4 considers the analysis of ADDT data for the purposes of estimating a thermal index. The current industry standards use a two-step procedure involving least squares regression in each step. The methodology preferred in the statistical literature is the maximum likelihood procedure. A comparison of the procedures is performed and two published datasets are used as motivating examples. The maximum likelihood procedure is presented as a more viable alternative to the two-step procedure due to its ability to quantify uncertainty in data inference and modeling flexibility. / Ph. D.

As the world's nuclear reactors approach the end of their originally planned lifetimes and seek license extensions, which would allow them to operate for another 20 years, accurate information regarding neutron radiation exposure is more important than ever. Structural components such as the reactor pressure vessel (RPV) become embrittled by neutron irradiation, reducing their capability to resist crack growth and increasing the risk of catastrophic failure. The current dosimetry approaches used in these high flux environments do not provide real-time information. Instead, radiation dose is calculated using computer simulations, which are checked against dose readings that are only available during refueling once every 1.5-2 years. These dose readings are also very expensive, requiring highly trained technicians to handle radioactive material and operate specialized characterization equipment. This dissertation describes the development of a novel neutron radiation dosimeter based on carbon nanotubes (CNTs) that not only provides accurate real-time dosimetry, but also does so at very low cost, without the need for complex instrumentation, highly trained operators, or handling of radioactive material. Furthermore, since this device is based on radiation damage rather than radioactivation, its readings are time-independent, which is beneficial for nuclear forensics. In addition to development of a novel dosimeter, this work also provides insight into the particularly under-investigated topic of the effects of neutron irradiation of carbon nanotubes. This work details the fabrication and characterization of carbon nanotube based neutron and gamma radiation dosimeters. They consist of a random network of CNTs, sealed under a layer of silicon dioxide, spanning the gap between two electrodes to form a conductive path. They were fabricated using conventional wafer processing techniques, making them intrinsically scalable and ready for mass production. Electrical properties were measured before and after irradiation at several doses, demonstrating a consistent repeatable trend that can be effectively used to measure dose. Changes to the microstructure were investigated using Raman spectroscopy, which confirmed that the changes to electrical properties are due to increasing defect concentration. The results outlined in this dissertation will have significant impacts on both the commercial nuclear industry and on the nanomaterials scientific community. The dosimeter design has been refined to the point where it is nearly ready to be deployed commercially. This device will significantly improve accuracy of RPV lifetime assessment while at the same time reducing costs. The insights into the behavior of CNTs in neutron and gamma radiation environments is of great interest to scientists and engineers studying these nanomaterials. / Ph. D.

This thesis presents an investigation into the characteristics of and driving methods for light emitting diode (LED) lamp system. A comprehensive overview on the lighting development is proposed. The characteristic of the light emitting diode (LED) lamp is described and the requirements of the ballast for the light emitting diode (LED) lamp are presented. Although LED lamps have longer lifetime than fluorescent lamps, the short lifetime limitation of LED driver imposed by electrolytic capacitor has to be resolved. Therefore, an LED driver without electrolytic capacitor in the whole power conversion process is preferred. In this thesis, a single phase, power factor correction converter without electrolytic capacitors for LED lighting applications is proposed, which is a modified SEPIC converter working in discontinuous conduction mode (DCM). Different with a conventional SEPIC converter, the middle capacitor is replaced with a valley-fill circuit. The valley-fill circuit could reduce the voltage stress of output diode and middle capacitor under the same power factor condition, thus achieving higher efficiency. Instead of using an electrolytic capacitor for the filter, a polyester capacitor of better lifetime expectancy is used. An interleaved power factor correction SEPIC with valley fill circuit is proposed to further increase the efficiency and to reduce the input and output filter size and cost. The interleaved converter shows the features such as ripple cancellation, good thermal distribution and scalability. / Master of Science

Environmental fluctuations in operating fuel cells impose significant biaxial stresses in the constrained proton exchange membranes (PEM). The PEM's ability to withstand cyclic environment-induced stresses plays an important role in membrane integrity and consequently, fuel cell durability. In this thesis, pressure loaded blister tests are used to study the mechanical durability of Gore-Select® series 57 over a range of times, temperatures, and loading histories. Ramped pressure tests are used with a linear viscoelastic analog to Hencky's classical solution for a pressurized circular membrane to estimate biaxial burst strength values. Biaxial strength master curves are constructed using traditional time-temperature superposition principle techniques and the associated temperature shift factors show good agreement when compared with shifts obtained from other modes of testing on the material. Investigating a more rigorous blister stress analysis becomes nontrivial due to the substantial deflections and thinning of the membrane. To further improve the analysis, the digital image correlation (DIC) technique is used to measure full-field displacements under ramped and constant pressure loading. The measured displacements are then used to validate the constitutive model and methods of the finite element analysis (FEA). With confidence in the FEA, stress histories of constant pressure tests are used to develop linear damage accumulation and residual strength based lifetime prediction models. Robust models, validated by successfully predicting fatigue failures, suggest the ability to predict failures under any given stress history whether mechanically or environmentally induced - a critical step in the effort to predict fuel cell failures caused by membrane mechanical failure. / Master of Science

This study examined the cumulative, more insidious, impact of repeated drinking on sober-state aggression based on research that has pointed to the negative neural effects of chronic alcohol consumption, especially on frontal lobe functioning. In particular, it examined the relationship between repeated alcohol use and sober-state reactive aggression as it is mediated or moderated by sober-state executive cognitive functioning (ECF), thus expanding upon research that has examined the relationship between acute alcohol intoxication and consequent aggression while under the influence (Giancola, 2000b). It was hypothesized that ECF would mediate the relationship between repeated alcohol use and sober-state reactive aggression in college students in that a history of alcohol use would lower sober-state ECF which in turn would increase sober-state impulsive aggression in individuals. It was further hypothesized with a moderational model that high levels of ECF would offset the more insidious effects of repeated alcohol use on subsequent sober-state aggressive acts. Moreover, those effects would remain after controlling for potential confounds of violence exposure, gender, and intelligence. Eighty college students, aged 18-23 years, from Virginia Tech were recruited to participate in this study. A self-report measure for aggression, neuropsychological tests for ECF, and a lifetime drinking interview schedule were used to assess the relationship between cumulative alcohol use, sober ECF, and sober aggression. A combination of bivariate and hierarchical regression analyses was used to analyze the data. The hypotheses of this study were not supported. Instead, the results supported a positive relationship between prior exposure to violence and later escalation of alcohol use and perpetrated violence. Additionally, these results support the presence of a "binge drinking" pattern within the sample. / Master of Science