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Isolation, identification, and characterization of phosphoribosyl-amino-imidazole-succinocarboxamide synthetase from Neurospora crassaFisher, Charles Ray. Brockman, Herman E. January 1968 (has links)
Thesis (Ph. D.)--Illinois State University, 1968. / Title from title page screen, viewed Aug. 17, 2004. Dissertation Committee: H.E. Brockman (chair), J.L. Frehn, C.W. Hardiman, A.E. Liberta, J.E. Perham, D.D. Pittman. Includes bibliographical references (leaves 61-65). Also available in print.
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A functional analysis of the mammalian E3 ubiquitin ligase WWP1 in a yeast modelSankaran, Saumya M. January 2009 (has links)
Thesis (M.S.)--Brandeis University, 2009. Senior honors thesis--Brandeis University, 2009. / Title from PDF title page (viewed on June 29, 2009). Includes bibliographical references.
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Synthesis and characterization of a DNA ligase : towards two stage replication /Ye, Jingdong. January 2001 (has links)
Thesis (Ph. D.)--University of Chicago, Dept. of Chemistry, 2002. / Includes bibliographical references (p. 157-158). Also available on the Internet.
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Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase with group I intronsChen, Xin. January 2002 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2002. / Vita. Includes bibliographical references. Available also from UMI Company.
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Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase with group I intronsChen, Xin 18 April 2011 (has links)
Not available / text
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S-adenosylmethionine synthetase activity during spore germination in Mucor racemosusFoss, Kathryn January 1990 (has links)
When introduced into nutrient medium under air, the asexual sporangiospores of Mucor racemosus germinated within 7 to 8 hours ending in the emergence of germ tubes. Sadenosylmethionine synthetase (SAM synthetase) activity was found to be present in the dormant spore, and increased at a rapid rate after 3 hours. The inhibition of enzyme activity by cycloleucine early in the germination process completely inhibited germ tube formation and only rounded, swollen spores were seen at the end of 7.5 hours. De novo protein synthesis was found to be necessary for SAM synthetase activity throughout the germination process. De novo RNA synthesis was found to be unnecessary for the increase in enzyme activity during the first 3 hours. This suggests that SAM synthetase is coded for in the stored mRNA of the spore. General protein methylation was found to increase through the first 4.5 hours of germination. Autoradiographs of post-translationally methylated proteins subjected to polyacrylamide gel electrophoresis revealed approximately 8 bands which appear to change significantly in the degree of methylation during the 7.5 hour germination process. None of the bands were detectable until the 4.5hour period, which corresponds to the rapid increase in enzyme activity seen after 3 hours. / Department of Biology
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Characterization of an Orf virus RING-H2 protein, B5L : a mimic of cellular anaphase promoting complex subunit 11Mo, Min, n/a January 2009 (has links)
The anaphase promoting complex (APC/C) is an ubiquitin ligase that is an essential regulator of multiple steps in the cell cycle. The complex consists of at least 12 subunits with a catalytic core formed by a scaffold protein, APC2, and a RING-H2 protein, APC11. The Parapoxvirus, Orf virus (OV), encodes a RING-H2 protein, B5L, with clear sequence similarities to APC11. The disruption of APC/C function leads to pre-mature entry into S phase and a delayed M phase exit and, potentially, apoptosis. This investigation explored the functional significance of the similarity between B5L and APC11 and specifically sought to determine if B5L manipulates cell cycle regulation by targeting APC/C function.
Co-immunoprecipitation experiments from lysates of cells expressing a range of constructs revealed an interaction between B5L and APC2 in the same manner as seen with APC11. Furthermore, B5L was found to associate with endogenous APC/C. However, although APC11 promoted the formation of polyubiquitin chains in substrate-independent in vitro assays, B5L was inactive in this assay. Bioinformatics comparisons of APC11 and other known RING ubiquitin ligases with B5L and its poxviral homologues revealed some subtle differences. In particular a domain of APC11 (amino acids 61-74), that is essential for its ubiquitin ligase activity is not conserved in B5L or its homologues. When this APC11 domain was incorporated in place of the corresponding region of B5L (amino acids 59-67), the mutated B5L acquired ubiquitin ligase activity. On the other hand, APC11 protein in which the domain was replaced with that of B5L lost ubiquitin ligase activity.
Stable cell lines expressing B5L showed an increased number of cells in G2/M phase (30�4%) compared with cell lines expressing APC11 (11�2%, n=3, p<0.05, ANOVA, Tukey�s), consistent with impaired APC/C function. APC/C substrates such as cyclin A, cyclin B and the thymidine kinase were stablized in B5L-expressing cells compared with control cells. Furthermore, transient hyper-expression of B5L induced apoptosis in 25�2% (n=3, p<0.05) of the cell population compared with only 6�1% apoptotic cells when APC11 was hyper-expressed. Analysis of the DNA content of OV-infected cells revealed enhanced DNA synthesis compared with cells infected with a B5L knockout OV.
These observations indicate that B5L is a non-functional mimic of APC11. It associates with APC/C, but lacks ubiquitin ligase activity, and hence disrupts APC/C function. These abilities may enable OV to induce a cellular environment that enhances viral replication.
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Characterization of an Orf virus RING-H2 protein, B5L : a mimic of cellular anaphase promoting complex subunit 11Mo, Min, n/a January 2009 (has links)
The anaphase promoting complex (APC/C) is an ubiquitin ligase that is an essential regulator of multiple steps in the cell cycle. The complex consists of at least 12 subunits with a catalytic core formed by a scaffold protein, APC2, and a RING-H2 protein, APC11. The Parapoxvirus, Orf virus (OV), encodes a RING-H2 protein, B5L, with clear sequence similarities to APC11. The disruption of APC/C function leads to pre-mature entry into S phase and a delayed M phase exit and, potentially, apoptosis. This investigation explored the functional significance of the similarity between B5L and APC11 and specifically sought to determine if B5L manipulates cell cycle regulation by targeting APC/C function.
Co-immunoprecipitation experiments from lysates of cells expressing a range of constructs revealed an interaction between B5L and APC2 in the same manner as seen with APC11. Furthermore, B5L was found to associate with endogenous APC/C. However, although APC11 promoted the formation of polyubiquitin chains in substrate-independent in vitro assays, B5L was inactive in this assay. Bioinformatics comparisons of APC11 and other known RING ubiquitin ligases with B5L and its poxviral homologues revealed some subtle differences. In particular a domain of APC11 (amino acids 61-74), that is essential for its ubiquitin ligase activity is not conserved in B5L or its homologues. When this APC11 domain was incorporated in place of the corresponding region of B5L (amino acids 59-67), the mutated B5L acquired ubiquitin ligase activity. On the other hand, APC11 protein in which the domain was replaced with that of B5L lost ubiquitin ligase activity.
Stable cell lines expressing B5L showed an increased number of cells in G2/M phase (30�4%) compared with cell lines expressing APC11 (11�2%, n=3, p<0.05, ANOVA, Tukey�s), consistent with impaired APC/C function. APC/C substrates such as cyclin A, cyclin B and the thymidine kinase were stablized in B5L-expressing cells compared with control cells. Furthermore, transient hyper-expression of B5L induced apoptosis in 25�2% (n=3, p<0.05) of the cell population compared with only 6�1% apoptotic cells when APC11 was hyper-expressed. Analysis of the DNA content of OV-infected cells revealed enhanced DNA synthesis compared with cells infected with a B5L knockout OV.
These observations indicate that B5L is a non-functional mimic of APC11. It associates with APC/C, but lacks ubiquitin ligase activity, and hence disrupts APC/C function. These abilities may enable OV to induce a cellular environment that enhances viral replication.
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Studies on succinic thiokinaseCha, Sungman, January 1963 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1963. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Inhibition and functional characterization of asparagine synthetaseGutierrez, Jemy A. January 2006 (has links)
Thesis (Ph. D.)--University of Florida, 2006. / Title from title page of source document. Document formatted into pages; contains 119 pages. Includes vita. Includes bibliographical references.
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