Zugl.: Potsdam, Univ., Diss., 2008

Zugl.: Passau, Univ., Habil.-Schr., 2006

No description available.

No description available.

No description available.

No description available.

No description available.

This diploma thesis contains a literary overview including the basic characteristics of metallothioneins, a summary of their functional properties and the cell processes affected by them. The practical part of the thesis deals with the issue of metallothioneins as potential tumour markers using the multiplex RT-PCR method, for which specific primers were designed, with the subsequent sequencing of their products. The following step involved a study of expression using the PCR method for selected MT1A, MT2A and MT3 genes after treating cell lines with cisplatin. Experiments were carried out on PNT1A, LNCaP and DU145 prostatic cell lines. Despite a very high similarity of the individual isoforms of metallothioneins, a triplex of three genes for two cell lines, PNT1A and DU145, was created. Upon a statistical evaluation of data obtained via the method of polymerase chain reaction in real time, it was found that, after the application of cisplatin, there was an increase in metallothionein expression.

Inaug.-diss.--Greifswald. / Lebenslauf. At head of title: Geographie. "Literatur und quellenangaben": p. 68-74.

The aim of the thesis is to study differences between sexes of rats in tumour growth, the difference in immune response between rats with progressing and spontaneously regressing tumours compared to control rats and verification of immunological memory. We used Lewis rat model with subcutaneously inoculated sarcoma. Peripheral blood of males showed a higher number of red blood cells, a higher concentration of haemoglobin and higher haematocrit compared to females. Experimental females with inoculated sarcoma had higher levels of CD161 (NK) cells at the beginning of experiment in comparison with the experimental and control males and control females. A strong infiltration of NK cells was observed in tissue sections of spontaneously regressing sarcoma compared to progressing tumours tissue. Important factors that can influence if the animal will show tumour progression or spontaneous regression are neutrophil granulocytes (they can induce both tumour-supporting and anti-tumour response), vascularization and extent of necrosis in tumour tissue. We have verified immunological memory in a small group of females by the secondary inoculations of the same tumour cells after complete disappearance of primary, spontaneously regressing tumour. No sarcoma repeatedly evolved in any of the females. Results obtained in the present work brought new insights into the problems of tumour in the Lewis rat sarcoma model with inoculated sarcoma. These results may have significance also for area of clinical oncology due to similarities with the behaviour of some human cancers.