A long period fiber grating (LPFG) is formed by inducing the periodic refractive index variation along a fiber. A lot of work has been done to fabricate the LPFGs in the photonic crystal fibers (PCFs) to function as all-fiber band-rejection filters, interferometers, and sensing applications. In this thesis, we propose a novel LPFG based on the gel-filled PCF. The PCF filled with the UV gel was exposed to the high-intensity UV light through the mask. The periodic index variation is formed along the fiber in the cladding region, resulting in the LPFG. By measuring the propagation losses of our LPFG, three spectral dips in the transmission bands are observed at 872 nm, 1309 nm, and 1418 nm as the grating period is 600 £gm, which indicates the mode coupling from the fundamental core mode to the higher order modes (HOMs) of the gel-filled PCFs. By using a full-vector finite-difference frequency-domain (FDFD) method, we numerically calculate the phase match condition for our LPFGs. The calculated resonant wavelengths are 875 nm, 1319 nm, and 1415 nm. Very good agreement between the measured resonant wavelengths and the numerical results is obtained. We also fabricate the selectively gel-filled LPFGs to reduce the propagation losses by utilizing a simple selectively blocking technique. In addition, we measure and discuss the sensing sensitivities of the UV-induced LPFGs, including the temperature, strain, curvature, torsion, and surrounding refractive index (SRI) sensitivities. The measured sensitivity to temperature is 1.7 nm/¢XC from 25 ¢XC to 45 ¢XC. As the surrounding refractive index is increased to 1.377, the dip position has a maximum shift of 2 nm. Compared with other LPFGs, the UV-induced LPFGs are more insensitive to bending and strain due to the complete cladding structure. This could benefit the stability of the temperature sensors, based on our UV-induced LPFGs.

Using Long-Period Fiber Gratings as component of Gain-Flattening filter ofOptical Amplifier, and discuss spectrum of different struture of Long-Period Fiber Gratings. To investigate the spectra characteristics of Long-Period fiber Gratings for designing reference resources. Aiming at different Gain-Flattening filter uses different struture of fiber grating, and the decision of parameter of fiber grating using Genetic Algorithm. Finally delcaring the dataflow of designing filter using Long-Period Fiber Grating and conclusion.In this paper , gain flatttening Cr:YAG optical amplifier spectrum and Erbium-doped optical amplifier spectrum,one achieve 300nm bandwidth and another achieve 40nm.

Recently, long-period fiber gratings (LPFGs) based on PCFs have been demonstrated by using heating or a mechanically pressure to induce periodic index variations along the fibers. However, LPFGs fabricated by these two methods suffer the structure damage. In this thesis we propose novel optically controllable LPFGs based on the photoresponsive photonic liquid crystal fibers (PLCFs) and no structure damage occurs during the fabrication process. The photoresponsive PLCF was filled with a LC mixture consisting of the nematic LC E7 and the photoresponsive 4MAB. The properties of the photoresponsive PLCF can be modulated by using laser irradiation. In addition, the transmission bands of the photoresponsive PLCF can also be tuned by controlling the 4MAB concentration or operation temperature. An optically controllable LPFG was fabricated based on the photoresponsive PLCF by using blue-laser irradiation through a mask with 700-£gm grating period. The measured resonant wavelength appeared at 1539 nm with the FWHM was 27 nm, and the maximum dip depth was about −15 dB with a 6.5-dB insertion loss. The LPFG was shown to be erasable by using a green laser. In addition, we have also investigated the effects of the number of grating period, 4MAB concentrations, operation temperatures, thermal recovery properties, and irradiation intensity on the LPFGs. Our proposed optically controllable LPFGs possess reversible property and are quite useful to be applied in tunable optical devices.

The diploma thesis described the basic principles and characters of the long period fiber gratings (LPFGs). Our concern was to describe basic mathematical description of these gratings needed to their modelation and simulation. Consequently some exact models leading to changes in the shape of the spectrum LPFGs were suggested, these methods were the chirping, apodization and changes in the average refractive index navg. In the Matlab environment, programes for counting the important parametres LPFGs were created and meanwhile each model leading to the change in the shape of the spectrum was simulated.

<p> Long-period fiber grating (LPG) sensors have been widely used as refractive index sensors due to their high sensitivity to the ambient refractive index change surrounding the fiber cladding of the LPG. Application of the LPG refractive index sensor has been found in chemical sensing and biochemical sensing, however for application of label-free dip and measure biosensors based on receptor immobilized LPG bio-sensor, the conventional fiber optic refractive index sensors are limited in the refractive index sensitivity, resolution, and operational range owing to the low sensitivity of the cladding mode effective index dependence on the ambient refractive index and the broad-spectrum feature of the LPG transmission spectrum. Low-cost, disposable fiber optic biochemical sensors with improved sensitivity, stability and resolution are needed to provide a high-sensitivity platform for immunology and DNA/aptamer biosensor. </p> <p> In this work, a novel fiber optic biosensing platform based on the LPG and the LPG in-fiber Michelson interferometer is designed and fabricated. The sensitivity and operation range enhancement is optimized by modifying the fiber cladding structure through reducing the cladding layer radius and applying a high-refractive index overlay with appropriate refractive index and thickness. The resolution of the refractive index sensor is improved by adopting the LPG in-fiber Michelson interferometer which turns the wide-spectrum feature of the LPG transmission spectrum into a narrow spectrum feature on the reflection spectrum of the interferometer. The reflection spectrum nature of the LPG in-fiber Michelson interferometer turns the sensor head into a single-end optotrode. The optotrode coated with bio-recognition film thus physically constitutes a short piece of fiber with one section of cladding reduced fiber. With single strand DNA (ssDNA) immobilized on the surface of the fiber cladding through biotin-avidin bridge, detection of the antisense DNA for the immobilized ssDNA is demonstrated. Immunoassay based on capture of target antigen by covalently immobilized antibody shows that reduction of the fiber cladding not only improve the sensitivity of the long period grating in-fiber Michelson interferometric biosensor but also improves the assay time. </p> / Thesis / Doctor of Philosophy (PhD)

No description available.

abstract: Biological membranes are critical to cell sustainability by selectively permeating polar molecules into the intracellular space and providing protection to the interior organelles. Biomimetic membranes (model cell membranes) are often used to fundamentally study the lipid bilayer backbone structure of the biological membrane. Lipid bilayer membranes are often supported using inorganic materials in an effort to improve membrane stability and for application to novel biosensing platforms. Published literature has shown that a variety of dense inorganic materials with various surface properties have been investigated for the study of biomimetic membranes. However, literature does not adequately address the effect of porous materials or supports with varying macroscopic geometries on lipid bilayer membrane behavior. The objective of this dissertation is to present a fundamental study on the synthesis of lipid bilayer membranes supported by novel inorganic supports in an effort to expand the number of available supports for biosensing technology. There are two fundamental areas covered including: (1) synthesis of lipid bilayer membranes on porous inorganic materials and (2) synthesis and characterization of cylindrically supported lipid bilayer membranes. The lipid bilayer membrane formation behavior on various porous supports was studied via direct mass adsorption using a quartz crystal microbalance. Experimental results demonstrate significantly different membrane formation behaviors on the porous inorganic supports. A lipid bilayer membrane structure was formed only on SiO2 based surfaces (dense SiO2 and silicalite, basic conditions) and gamma-alumina (acidic conditions). Vesicle monolayer adsorption was observed on gamma-alumina (basic conditions), and yttria stabilized zirconia (YSZ) of varying roughness. Parameters such as buffer pH, surface chemistry and surface roughness were found to have a significant impact on the vesicle adsorption kinetics. Experimental and modeling work was conducted to study formation and characterization of cylindrically supported lipid bilayer membranes. A novel sensing technique (long-period fiber grating refractometry) was utilized to measure the formation mechanism of lipid bilayer membranes on an optical fiber. It was found that the membrane formation kinetics on the fiber was similar to its planar SiO2 counterpart. Fluorescence measurements verified membrane transport behavior and found that characterization artifacts affected the measured transport behavior. / Dissertation/Thesis / Ph.D. Chemical Engineering 2011

Biosensors have a multitude of important applications in basic research, environmental monitoring, biodefense, and medicine. This research aims to show that Ionic Self-Assembled Multilayers (ISAMs) adsorbed on Long Period Gratings (LPGs) can serve as a highly sensitive, robust, inexpensive optical-based biosensor platform. The ISAM technique is a layer-by-layer deposition method that builds nanometer-thick films based on the principle of Coulomb attraction between oppositely charged polyelectrolyte solutions while LPGs cause strong attenuation bands that enable an optical fiber to be extremely sensitive to changes in the surrounding environment. LPGs have been shown to be highly sensitive to the adsorption of nanoscale self-assembled films on the optical fiber cladding surface. In this work, we utilize Turnaround Point (TAP) LPGs, which possess even greater sensitivity than standard LPGs. This thesis focuses on evaluation of approaches to increasing the sensitivity of the sensor platfom, implementation of a biosensor for detection of several biomolecules, and preliminary evaluation of the potential for pH sensing. For a thin-film coated TAP LPG, we have demonstrated that shifts in the transmitted light intensity at the resonant wavelength of the LPG can result from the variation in film thickness and/or refractive index. We have observed decreases in intensity as large a 7 dB for one bilayer of ISAM film (~1 nm), which corresponds to an 80% decrease in the transmitted light intensity at the resonant wavelength. We have also shown that the sensitivity of the TAP LPG sensor can be increased by implementing nm-thick ISAM films that have a refractive index greater than silica. Furthermore, it is shown that incorporation of silica nanoparticles into the ISAM films significantly increases sensitivity through increased surface area and thickness. The biotin-streptavidin system was used as a model for implementaion and optimization of the ISAM-coated TAP LPG biosensor platform. Through evaluation of various biotin derivatives to maximize the amount functionalized onto the ISAM film, optimization of the ISAM film properties, and use of LPGs designed for higher sensitivity, the minimum detectable concentration of streptavidin was decreased from 0.0125 mg/ml to 12.0 ng/ml. The biosensor platform was then tested on prostate specific antigen (PSA), which is used as a clinical marker for early diagnosis of potential prostate cancer. Using a direct crosslinking approach of the monoclonal antibody to PSA into the ISAM film, a sensitivity level of 11.64 ng/ml PSA was obtained through combined optimization of the ISAM film and antibody surface coverage. Finally, the potential of ISAM TAP LPGs as pH sensors was examined based on the pH dependent swelling of ISAM films. / Ph. D.

Biosensors have widespread applications in many areas. Currently the Surface Plasmon Resonance (SPR) biosensor is one of the most prevalent types of biosensor. However, it has several disadvantages such as being delicate, expensive, and non-portable. Ionic Self-Assembled Multilayers (ISAMs) adsorbed on Long Period Fiber Gratings (LPGs) provides an attractive platform for building optical sensors, which could potentially overcome the disadvantages of SPR biosensors. The ISAM technique is a type of layer-by-layer deposition technique for building nanoscale thin films. An LPG is a type of fiber device that is sensitive to physical property changes of the ambient environment. LPGs have been extensively investigated for use as optical sensors. We have carried out a study on combining these two techniques to build efficient biosensors. In this thesis, we demonstrate ultra-sensitive LPGs whose attenuation can be changed by 25 dB (~99.7%) over a 48-nm spectral band, with ambient-index changes of only 2.7E-4. The device schematic allows arbitrarily high index sensitivities to be achieved, which makes it an attractive platform for realizing sensors and modulators that respond to small index changes. For a thin-film coated LPG, we find theoretically that the resonant wavelength shift of the LPG can result from either the variation of the thickness of the film and/or the variation of its refractive index. Furthermore, results illustrate that the sensitivity of the sensor could be enhanced using a nm-thick thin-film (e.g. ISAM films) whose refractive index is greater than silica. Experimentally, we demonstrate the fabrication of nm-thick ISAM films deposited on LPGs, which induces dramatic shifts in the resonant wavelength. The refractive index and the thickness of the ISAM film was precisely controlled by altering the relative fraction of the anionic and cationic materials combined with layer-by-layer deposition. Finally, we demonstrate that ISAM-coated LPGs can function effectively as biosensors by using the biotin-streptavidin system. These demonstrations confirm that the ISAM-LPG scheme provides a thermally-stable, reusable, and robust platform for building efficient optical sensors. / Ph. D.

In der medizinischen Diagnostik, Bioverfahrenstechnik und Umwelttechnik besteht ein steigender Bedarf an kompakten Analysegeräten für die schnelle Vor-Ort-Detektion spezifischer biochemischer Substanzen. Im Rahmen der Arbeit wurde daher ein neuartiger faseroptischer Sensor entwickelt, der in der Lage ist kleinste Brechzahländerungen, z.B. durch molekulare Bindungsprozesse, zu detektieren. Die hohe Empfindlichkeit an der vergoldeten Spitze der Sensorfaser beruht auf der Oberflächenplasmonenresonanz (SPR) einer einzelnen Mantelmode, die durch ein langperiodisches Fasergitter (LPG) ermöglicht wird. Die Übertragungsfunktion des Sensors wurde unter Verwendung eines Schichtwellenleitermodells schnell und präzise modelliert. Es konnte gezeigt werden, dass in einem wässrigen Umgebungsmedium die höchste Empfindlichkeit im Spektralbereich um 660 nm unter Annahme einer rund 35~nm dicken und 2~mm langen Goldbeschichtung erreicht wird. Weiterhin wurde nachgewiesen, dass mit einer intermediale Schicht aus Cadmiumsulfid die SPR der Mantelmode in einen höheren Spektralbereich verschoben und damit die Empfindlichkeit deutlich verbessert werden kann. Um eine geringe Polarisationsabhängigkeit des Sensors sicherzustellen, wurde ein nasschemisches Abscheidungsverfahren für die allseitige Goldbeschichtung der Sensorfaser entwickelt. Die spezifischen optischen Eigenschaften dieser Abscheidungen wurden mit Hilfe von LPGs untersucht, die durch eine spezielle UV-Belichtung hergestellt wurden. Die Experimente ergaben, dass die komplexe Permittivität nasschemischer Abscheidungen mit Schichtdicken oberhalb von 50~nm mit aufgedampften Goldschichten vergleichbar ist. Die Verluste der adressierten Mantelmoden wurden mit einer äquivalenten Sensoranordnung aus zwei identischen LPG untersucht. Dabei konnte ein Skalierfaktor abgeleitet werden, der die effiziente Berechnung der Mantelmodendämpfung erlaubt. Es wurde nachgewiesen, dass die Brechzahlauflösung etablierter volumenoptischer SPR-Sensoren mit einer einfachen Transmissionsmessung an einer geeigneten Wellenlänge erreicht werden kann. Die äußerst kompakte Sensorfläche des faseroptischen Sensors ermöglicht darüber hinaus die Untersuchung deutlich kleinerer Probenvolumina ohne ein zusätzliches mikrofluidisches System zu benötigen. Es wurde gezeigt, dass sekundäre Brechzahländerungen aufgrund von Temperaturschwankungen oder unspezifische Ablagerungen durch eine differentielle Auswertung zweier identischer Sensoren kompensiert werden können. Die verbleibende Querempfindlichkeit wird durch die Polarisationsabhängigkeit der Sensoren bestimmt. Die geringste Querempfindlichkeit konnte daher mit einer homogenen nasschemischen abgeschiedenen Sensorfläche nachgewiesen werden. / Compact analysis devices which facilitate the rapid detection of specific biochemical substances are in increasing demand in the fields of point-of-care medical diagnostics, bioprocess engineering and environmental engineering. The aim of this work was therefore to design a novel fiber-optic sensor able to detect small refractive index changes such as those caused by molecular binding processes. The high level of sensitivity at the gold-plated tip of the sensor fiber stems from the surface plasmon resonance (SPR) of a single cladding mode, which is the result of a long-period fiber grating (LPG). The transfer function of the sensor was calculated quickly and accurately using a slab waveguide model. It was observed that the highest level of sensitivity in an aqueous ambient medium is achieved at a wavelength of 660 nm assuming a gold coating of 35 nm in thickness and 2 mm in length. Furthermore, it was demonstrated that an intermedial cadmium sulfide layer shifts the SPR of the cladding mode towards higher wavelengths, thus leading to significantly enhanced sensitivity. An electroless plating process for the omnidirectional deposition of gold on the sensor fiber was developed in order to minimize the sensor\'s dependency on polarization. The specific optical properties of the gold layer deposited were investigated with the aid of LPGs fabricated using a special UV exposure method. The experiments showed the complex permittivity of electroless platings with a thickness of over 50 nm to be comparable with that of evaporated gold layers. The losses of the addressed cladding modes were investigated using an equivalent sensor setup consisting of two identical LPGs. This facilitated the determination of a scaling factor enabling the effcient calculation of cladding mode attenuation. It was demonstrated that it is possible to obtain the refractive index resolution of established volume optical SPR sensors with the aid of simple transmission measurements at a specific wavelength. Moreover, the extremely compact sensing area of the fiber-optic sensor enables the investigation of smaller sample volumes without the need for an additional microfluidic system. Secondary refractive index changes caused by temperature fluctuations or unspecific binding events can be compensated for by means of the differential interrogation of two identical fiber-optic sensors. The residual cross sensitivity is determined by the polarisation dependency of the sensor. The lowest cross sensitivity was therefore demonstrated in combination with a homogeneous electroless plated sensor surface.