Two indoor feeding experiments were conducted at the Animal Physiology Unit (APU) of Massey University, involving young sheep, to investigate the effect of feeding forage willow upon the death of established parasites and upon parasite fecundity, using chaffed lucerne as the control diet. Experiment 1: Twenty-four (24) parasite-free weaned hoggets weighing 29.9 ±1.2 kg (SD) were individually penned and fed chaffed lucerne ad libitum during a preexperimental adaption period of 5 weeks. They were then fed either lucerne chaff or chopped willow for a further 5 weeks (n = 12/group) and intakes were adjusted such that the DMI of the two groups was similar during weeks 9 & 10. All lambs were infected with L3 larvae parasites comprising 20,650 Teladorsagia, 1,320 Trichostrongylus and 330 Cooperia through oral drenching 12 days before willow feeding started. This was done after confirmation that the sheep were free of nematodes through FEC analysis. Total faeces were collected for 3 day periods towards the end of weeks 9 & 10, to measure diet digestibility and total faecal egg excretion. The sheep were slaughtered at the end of week 10. Voluntary feed intake (VFI), FEC and liveweight were measured weekly, whilst burdens of individual parasites and carcass characteristics were measured after slaughter. Duplicate samples of each feed offered and individual animal refusals were taken daily and pooled weekly per animal for chemical analysis. Female worm fecundity was calculated by two methods. Blood samples for immunological analysis were collected on days 20, 34, 51 and 70, and analysed for components of white blood cells (WBC) and for lymphocyte subsets. Experiment 2: A 2 x 2 changeover experiment was conducted, involving two time periods (Period 1 and Period 2 each of 14 days) with the same diets as used in Experiment 1, fed to 9 individually penned parasite-free young sheep randomly allocated to experimental diets. The parameters investigated were FEC and larvae hatching. Initially, a period of 7 days was allowed for acclimatisation in which both groups were fed on half willow and half lucerne chaff. This was followed by Period 1 with 4 lambs fed lucerne and 5 fed willow, after which the diets were changed over for Period 2. Total faeces produced were collected from all animals on the last day of each period using bagged sheep. A known number of Teladorsagia eggs (500 epg) was then added to faecal samples from these sheep and faeces-egg mixtures were made from which FEC was determined, to see if egg recovery was affected by these diets. Faecal samples for Period 2 with added eggs were also incubated for 10 days to measure hatchability. The recovery of added Teladorsagia eggs in Experiment 2 was 85% in lucerne-fed lambs and 53% willow-fed lambs (P<0.001); these were used as correction factors for Experiment 1 data. Larvae that hatched per gram of wet faeces in Experiment 2 tended to be lower for sheep fed willow than lucerne chaff (71% vs 83% of eggs added; P=0.08). Willow feed offered had lower DM (P<0.001) and CP (P<0.05) content, but had a significantly higher OM content (P<0.01) than lucerne chaff. Condensed tannin content of chopped willow was 27 g/kg DM, with only traces for lucerne. Apparent digestibility for DM (62.4% vs 59.5%; P≤0.05), OM (64.8% vs 59.9%; P≤0.001), DOMD (58.1% vs 55.0%; P≤0.01) and calculated ME (9.48 MJ/kg vs 8.96 MJ/kg; P≤0.01) were higher for the willow diet. VFI was similar for both groups during the adaption period (P>0.05) but declined with the introduction of willow in week 6 (P<0.001) and then progressively increased until it was similar to lucerne-fed sheep in weeks 9 & 10 (P>0.05). Calculated DM intake per head/day during the last two weeks of Experiment 1 was similar for the two groups (P>0.05); while the willow group had higher ME (P<0.01) and CP (P<0.001) intake per animal/day. Liveweight increased for the two groups during the adaption period (P>0.05), then declined for willow-fed lambs in week 6 (P<0.001) but later increased and by week 10 was similar to that of lucerne-fed lambs. The willow-fed lambs had lower carcass GR than the lucerne-fed lambs (P<0.01) when carcass weight was used as a covariate. Adjusted total daily egg production in Experiment 1 was lower in willow-fed sheep than lucerne-fed sheep, due to reductions for Haemonchus spp. (P<0.05) and Teladorsagia spp. (P<0.05). The per capita fecundity for Haemonchus worm spp. (P<0.05) and the in utero fecundity in both abomasal Teladorsagia spp. and small intestinal Trichostrongylus spp. (P<0.001) were lower for willow-fed sheep. There was reduced production of larvae for both Haemonchus spp. and Teladorsagia spp. (P<0.05) in willow-fed sheep. Feeding willow reduced the burden of Haemonchus adult worms in the abomasum (P<0.01) but reduced female worm burden only in Teladorsagia spp. (P<0.05) and reduced Cooperia spp. in the small intestines (P<0.01). Total WBC, total lymphocytes, subsets of lymphocytes and other white-cell groups were not affected by willow feeding (P>0.1). It was concluded that feeding chopped willow to young sheep reduced nematode worm burdens in the abomasum, especially both male and female Haemonchus spp., and reduced female worm burdens of Teladorsagia spp. Female worm fecundity of both species was also reduced by willow feeding. These reductions have been associated with CT content in the willow feed and the reduced worm burdens have been attributed to the death of the established worms by CT, since there was no evidence of immune priming in willow-fed sheep. Compounds present in the faeces of willow-fed sheep have been found to mask some of the nematode eggs, making them invisible by microscopic examination while keeping their viability. It is postulated that this could be due to binding of nematode eggs to insoluble CT associated with indigestible fibre in the faeces of willow-fed sheep. Conventional methods of measuring FEC therefore underestimated nematode eggs present in the faeces of willow-fed sheep and this needs to be checked for other CT-containing forages.

The effect of continuous monoculture of sorghum, mungbean, oats, greenpanic and luceren on the nitrogen status of a brown clay soil from Narayen, South East Queensland was studied using biological and chemical methods. the soil nitrogen status was estimated by using indices of nitrogen availability i.e. waterlogged incubation method as the biological method and 2 M KCL, phosphate-borate buffer and 0.01 M CaCl_2 as rapid chemical methods. Total soil nitrogen and organic carbon were also included as estimates of soil nitrogen status. The results obtained by the waterlogged (WL) method were used to evaluate the results obtained by the three rapid chemical methods. The results show that of the three rapid chemical methods 2 M KCL was the most closely correlated with the waterlogged incubation method. Total N and organic C were also highly correlated with the WL method and they may have value as indices of nitrogen availability. The available N, total N and organic C contents in the 0-5 cm and 5-15cm layer soils were consistently higher under greenpanic and lucerne than for the annual crop treatments. The available N contents as determined by the WL method in the 0-5 cm and 5-15 cm soil layers under greenpanic and lucerne ranged from 75.6 to 126.5 ug N/g soil and 53.2 to 106.7 ug N/g soil, respectively, whereas the values under the annual crop treatments ranged from 13.2 to 75.3 ug N/g soil and 25.6 to 66.1 ug N/g soil, respectively. The total N contents in the 0-5 cm and 5-15 cm layer soils under the greenpanic and lucerne ranged from 0.29 to 0.38 % and 0.20 to 0.33 %, respectively, whereas the values under the annual crop treatments ranged from 0.17 to 0.31 % and 0.17 to 0.25 %, respectively. The availability of the total N as indicated by the available N as a percentage of the total N in the 0-5 cm and 5-15 cm layer soils under the greenpanic and lucerne ranged from 2.6 to 4.0 % and 2.1 to 3.2 %, respectively, whereas the values under the annual crop treatments ranged from 0.8 to 3.8 % and 1.5 to 2.4 %, respectively. In addition the organic C contents in the 0.5 cm and 5-15 cm layer soils under the greenpanic and lucerne range from 2.4 to 4.0 % and 2.0 to 3.5 %, respectively, whereas the values under the annual crop treatments ranged from 1.4 to 3.3 % and 1.7 to 2.7 %, respectively.

Thesis (doctoral)--Universität, Zürich, 2001/2002. / Includes bibliographical references (p. 317-332).

Please read the abstract in the section, 00front of this document / Dissertation (MSc)--University of Pretoria, 2008. / Animal and Wildlife Sciences / MSc / Unrestricted

Thesis (MSc. Agriculture (Animal Production)) -- University of Limpopo, 2015 / A series of experiments were carried out to investigate the effect of replacing calf starter feed with lucerne leaf-meal (LLM) on diet intake, rumen degradation and growth of Holstein heifer calves. The treatments were calf starter pellets (PEL), a mixture of 65% calf starter concentrate pellets and 35% LLM (P65L35), and a mixture of 50% calf starter concentrate pellets and 50% LLM (P50L50). The first part of the study determined nutrient composition of lucerne leaf-meal (LLM), calf starter pellets and lucerne leaf-meal substituted diets. The experimental design was a completely randomised design (CRD). Lucerne leaf-meal had high protein (25% DM) and gross energy (GE) (16.2 MJ/kg DM) levels; low starch (0.2% DM) and fibre fractions. All diets had similar (P>0.05) crude protein (CP) and GE values. The concentrate diet was higher (P<0.05) in starch, ether extracts and fibre bound CP (neutral detergent insoluble crude protein). The second part of the study estimated the supply of energy and protein fractions and carbohydrate composition from LLM and the diets. A CRD was used. Lucerne leaf-meal had high energy density and protein supply with low unavailable fibre and protein contents. All the treatments had TDN above 80%. Non-fibre carbohydrate levels differed (P<0.05) across treatments. The energy fractions were similar (P>0.05) across all dietary treatments. However, Diets P65L35 and P50L50 had higher (P<0.05) soluble and non-fibre carbohydrates than PEL diet. The third part of the study determined in vitro degradation of LLM and the three dietary treatments using the ANKOM DaisyII incubator system. Lucerne leaf-meal had high in vitro dry matter (IVDMD), organic matter (IVOMD), crude protein (IVCPD) and neutral detergent fibre (IVNDFD) degradation. All diets had similar (P>0.05) IVDMD and IVOMD at 0, 4, 10 and 48 hours of incubation. Higher (P<0.05) IVNDFD, IVCPD and effective degradation (ED) were observed in Diets P65L35 and P50L50 than in Diet PEL. No differences (P>0.05) in IVNDFD and IVCPD were observed at 24 and 48 hours of incubation. The rate of degradation (‘c’) was similar (P>0.05) across all the diets. The data demonstrated that LLM diets had higher (P<0.05) degradation values than Diet PEL. vi The fourth part of the study determined the effects of replacing calf starter pellets with lucerne leaf-meal on diet intake, feed conversion ratio and growth of pre-weaned (21 to 42 days old calves) and transition (43 to 56 days old calves) Holstein heifer calves. The experimental design was a completely randomised design, with a total of 24 calves housed in individual pens. This study was divided into two experimental phases, namely, pre-weaning (Experiment 1) and transition (Experiment 2) phases. In each experiment, different calves were used. Body weights were taken weekly. The balance of ruminal nitrogen (% RNB) was predicted using Large Ruminant Nutrition System (LRNS) model. In Experiment 1 calves had free access to clean water and fed 4 litres/calf/day of unpasteurised milk. During the pre-weaning phase, differences (P<0.05) were observed in solid feed dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and starch intakes with Diet P50L50 having higher (P<0.05) intakes than Diets PEL and P65L35. Similarly, higher (P<0.05) % RNB and daily weight gains (ADG) were observed with calves on Diet P50L50. However, calves had similar (P>0.05) initial and final weights and feed conversion ratio (FCR). Calves in Experiment 2 were fed 2 litres/calf/day of unpasteurised milk. Calves were weaned at the age of 56 days. During the transition phase, calves on Diet HP50L50 had higher (P<0.05) CP intake and % RNB than those on HPEL and HP65L35 diets. However, higher (P<0.05) solid feed starch intake was observed with calves on Diet HPEL. All dietary treatments had similar (P>0.05) DM intake, initial and final live weights, ADG and FCR. The fifth part of the study predicted diet concentrations of Holstein heifer calves under specific conditions using the level 1 solution of Large Ruminant Nutrition System (LRNS) model. During the pre-weaning phase, P65L35 and P50L50 diets indicated higher (P<0.05) energy density values than Diet PEL. However, all diets had similar (P>0.05) metabolisable energy levels. No differences (P>0.05) in net energy for maintenance (NEm) and gain (NEg) during the transition phase were observed. However, Diet HPEL had higher (P<0.05) apparent TDN and ME levels compared to other dietary treatments. Diets P65L35 and P50L50 had higher (P<0.05) protein, energy density and degradation values than Diet PEL. It is concluded LLM inclusions in the calf diet improved Holstein heifer calves’ performance. / Agricultural Research Council (ARC)

This study examined the differences in the chemical composition, particularly fatty acids, of the lipid extracted from the fibre of bucks, does and castrated goats. The study provides a more detailed understanding of the chemical composition of buck fibre lipid and how it varies throughout the year, and also details the effect of body region and nutrition on the production and chemical composition of lipid from buck fibre. Lipid was extracted with either petroleum ether (non-polar) or chloroform/methanol azeotrope (polar) and analysed by gas chromatography and gas chromatography-mass spectrometry. The more polar solvent system extracted larger amounts of lipid and more of each individual fatty acid. The following buck specific ethyl branched fatty acids were identified: 2-ethylhexanoic, 4-ethylhexanoic, 2-ethyloctanoic, 4-ethyloctanoic, 6-ethyloctanoic, 2-ethyldecanoic, 4-ethyldecanoic, 2-ethyldodecanoic, 6-ethyldodecanoic, 4-ethyldodecanoic, 2-ethyltetradecanoic, 6-ethyltetradecanoic, 4-ethyltetradecanoic, 2-ethylhexadecanoic and 4-ethyloctadecanoic acids. Of these buck specific fatty acids only 4-ethylhexanoic (T), 4-ethyloctanoic, 4-ethyldecanoic, 4-ethyldodecanoic, 6-ethyldodecanoic (T), 4-ethyltetradecanoic, 2-ethylhexadecanoic (T) and 4-ethylhexadecanoic acids have been previously identified or tentatively identified (T) in buck fibre extracts. This shows that the chemical composition of buck fibre lipid is more complex than previously reported, and that it may be more difficult than previously thought to artificially duplicate the odour of the buck. Buck fibre samples had lower average concentrations of 2-methylpropanoic, 2-methylbutanoic, iso-pentadecanoic, anteiso-pentadecanoic, iso-hexadecanoic, anteiso-heptadecanoic, iso-octadecanoic and anteiso-nonadecanoic acids as compared with fibre samples from does, spayed does, or wethers that were castrated at one month of age. The reduced concentrations of these fatty acids in buck fibre extracts were likely to be due to the synthesis of ethyl branched derivatives of iso and anteiso fatty acids. Buck fibre samples had higher concentrations of benzoic acid as compared with fibre samples from does, spayed does, or wethers that were castrated at one month of age. The significance of these results is that non buck specific fatty acids may also make a contribution to the odour of bucks. When fibre samples were collected at various times throughout the year, it was found that the bucks had increased amounts of lipid and ethyl branched fatty acids in fibre samples shorn from March to September, as compared with fibre samples shorn in November and January. The increase in the amount of lipid and ethyl branched fatty acids corresponded with both the rutting period of the buck and the period when the buck odour was increased. This suggests that ethyl branched fatty acids could be pheromones. The variation in lipid content and fatty acid composition was also examined between fibre samples collected from different body regions of the buck during April, as alterations in sebaceous gland activity around the neck during rutting have been reported. It was found that the average amount of lipid in the neck region of the bucks was not statistically higher than the average amounts in the midside and hind regions. However, the ethyl branched fatty acid concentrations were statistically higher in the fibre from around the neck as compared with the fibre from the other body regions, which is consistent with the odour of the buck being most pronounced around the head and neck region. The lipid content and composition of fibre samples from bucks fed high and low quality diets (lucerne and pangola grass, respectively) was examined to determine the effect of nutrition on buck specific components. The high quality diet increased the amount of lipid and ethyl branched fatty acids in fibre samples collected in April from the neck, midside and hind regions, as compared with fibre samples from the corresponding body regions from bucks fed the low quality diet. Thus it may be possible for the pheromone levels of bucks to be increased by simply providing them with good nutrition. The lipid content and ethyl branched fatty acid concentrations of fibre samples increased earlier in the year for the lucerne fed bucks as compared with the pangola grass fed bucks. The lucerne fed bucks had increased concentrations of ethyl branched fatty acids in fibre samples shorn during December to June (6 months) whereas the pangola grass fed bucks had increased concentrations of ethyl branched fatty acids in fibre samples shorn during April to August (4 months). These observations show that good nutrition can result in both the earlier production of ethyl branched fatty acids and an extended period when ethyl branched fatty acids are produced. This suggests that nutrition can be used to manipulate pheromone levels in the buck. The period when the ethyl branched fatty acids were increased corresponded with the period when the plasma luteinizing hormone (LH) and testosterone concentrations, odour and sebaceous gland volume of the bucks were increased, which supports the assumption that ethyl branched fatty acids are involved in odour production and act as pheromones.

Feeding behaviour is controlled by the integration of both perceived cues from the plant and postingestive signals received by the animal. In general practice, dairy cattle are presented with a variety of feedstuffs throughout each day, in addition to changing feedstuffs over time. In the Australian context, the feeds generally include fodder (usually hay) and pasture whilst grazing, as well as various concentrates. For horses that are stabled for prolonged periods, their diet consists of fodder (again usually hay or chaff) and concentrates, in addition to the pasture consumed whilst allowed access to graze. In such situations, where animals are presented with a range of feedstuffs, some of them novel and for discrete periods of time, it is important that they readily accept the feeds when they are first presented to them. For both lactating dairy cows and performance horses, for example, it is important to avoid periods of low intake, as this can have immediate and sometimes longer-term consequences to animal performance. The daily rate of food intake is the single most important factor affecting animal performance and productivity (Illius et al. 2000) and an animal‟s responses to a feedstuff can be considered the ultimate measure of its quality. The animal‟s first response to the presentation of a new feed, which in this particular study was oaten or lucerne hay, is its level of voluntary intake, which depends in part on palatability. Palatability is an integrative term (Provenza 1995); to provide a quantitative measure of the acceptability of a feedstuff, a „preference value’ can be obtained by describing the preference of one hay relative to an alternate hay also on offer. The Australian fodder industry is increasingly adopting more objective measures of hay quality to improve marketing opportunities, especially in the export industry, and to meet the demands and expectations of local and overseas purchasers of hay. Being able to efficiently and reliably predict the preference value of any particular hay would be beneficial to processors, exporters, users (purchasers) of hay, and possibly plant breeders, to make more informed decisions. This thesis describes a comprehensive analysis of the chemical and physical characteristics of a selected number of oaten and lucerne hays and their relationship to the acceptability or „preference value‟ of the hays for lactating Holstein Friesian cows and Thoroughbred horses. The project aimed to (i) quantify preference values for a large number of oaten hays with dairy cows and horses and a similarly large number of lucerne hays with horses only, and (ii) develop predictive equations for animal preferences based on the chemical and physical properties of the hays and the animal‟s short-term rate of consuming the hays. A total of approximately 8,500 preference tests were conducted with 85 oaten hays offered to lactating Holstein Friesian cows and Thoroughbred horses, and 70 lucerne hays offered to Thoroughbred horses. The hays were selected to cover a range of nutritive values. The intake rate and preferences for all the hays were quantified in a series of tests replicated over time and with different animals. Each „trial‟ hay was offered with four „standard‟ hays (in separate tests). A suite of chemical (nutritive value) traits and physical traits were quantified for the hays. An assessment of the „odour profile‟ was also included in the characterisation of the hays. The nutritive, physical and volatile traits were then related to hay preference values in multiple and simple linear regression models and equations generated to predict hay preference values. A prediction of hay preference made directly from the spectra obtained by near infrared reflectance spectroscopy (NIRS) was also developed. Overall, the average preference value of an oaten hay (i.e., that obtained using the comparisons with all four standard hays) could be predicted from a range of nutritive value traits, typically the contents of acid detergent fibre (ADF), hemicellulose, crude protein (CP) and water soluble carbohydrates (WSC) - with a correlation co-efficient of about 79% with cows and 61% for horses. Acid detergent fibre and CP contents tended to have the biggest influence on preference value for both cows and horses, followed by WSC and hemicellulose content. Examination of lucerne hays offered to horses increased the correlation co-efficient to 74%, with ADF, hemicellulose and CP each having a similar magnitude of effect. This means that these traits, with an appropriate weighting for each, could account for up to about three-quarters of the variation in hay preference values for cows and horses. The in vitro digestibility of dry matter (IVD) of oaten and lucerne hay was nearly as accurate in predicting preference values as using the four nutritive value traits of ADF, hemicellulose, CP and WSC, which was anticipated as digestibility is a function of these chemical traits. Hay physical traits could also be used to predict preference values, although not as accurately as the nutritive value traits or IVD. Of the physical traits, shear energy had the largest effect (co-efficient approximately -1.15 compared to an average of -0.35 for the other traits in the equation). The use of NIRS to directly predict preference values was also encouraging, with the best calibration model yielding a correlation co-efficient of 61 to 81%, depending on the hay (oaten or lucerne) and the animal (cows or horses). Of the total 120 volatile compounds isolated from the oaten hays, six had some relationship with cow preferences and there were also six oaten hay volatiles related to horse preferences. Of the oaten hay volatiles significantly related to preference, four were positively related to cow preference whilst two were negatively related to cow preference. Similarly there were four volatiles positively related to horse preference and two consistently negatively related. Of the six oaten hay volatiles found to influence the preferences of dairy cows and horses, four were common to both species. Of the total of 147 volatile compounds isolated from the lucerne hays, 15 had some relationship with horse preference, with nine positively related to horse preference and six negatively related. The data reported here suggest that a predicted preference value for a hay can be generated using commonly measured quality traits, which would improve a buyer‟s confidence of animals responding favourably when first offered the hay. More specifically, for an average preference value, the most reliable prediction equation was based on nutritive value traits: ADF, hemicellulose, crude protein and water soluble carbohydrates solely. Alternatively a combination of nutritive and physical traits can be used to predict preference: in vitro digestibility, crude protein, water soluble carbohydrates and shear energy. The visible and near infrared spectra obtained by NIRS was also a promising method of prediction, and given the speed and affordability of NIRS, this technology could be further refined and used for routine measurement of predicted hay preference values. The volatile compounds shown to influence preference value of the hay should be identified and further research undertaken to investigate novel approaches to manipulate the preference of hays. Caution should be taken when attempting to use these prediction equations on a single specific hay in a single specific situation. The predictions and relationships investigated in this thesis are based on populations of hays and preferences of a group of animals and therefore some care should be taken when applying them to a particular situation with difference circumstances. Other factors that can influence feed preferences include an animal‟s feeding experiences, basal diet and diet history and various environmental factors unaccounted for here. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1349258 / Thesis (Ph.D.) - University of Adelaide, School of Agriculture, Food and Wine, 2008

Feeding behaviour is controlled by the integration of both perceived cues from the plant and postingestive signals received by the animal. In general practice, dairy cattle are presented with a variety of feedstuffs throughout each day, in addition to changing feedstuffs over time. In the Australian context, the feeds generally include fodder (usually hay) and pasture whilst grazing, as well as various concentrates. For horses that are stabled for prolonged periods, their diet consists of fodder (again usually hay or chaff) and concentrates, in addition to the pasture consumed whilst allowed access to graze. In such situations, where animals are presented with a range of feedstuffs, some of them novel and for discrete periods of time, it is important that they readily accept the feeds when they are first presented to them. For both lactating dairy cows and performance horses, for example, it is important to avoid periods of low intake, as this can have immediate and sometimes longer-term consequences to animal performance. The daily rate of food intake is the single most important factor affecting animal performance and productivity (Illius et al. 2000) and an animal‟s responses to a feedstuff can be considered the ultimate measure of its quality. The animal‟s first response to the presentation of a new feed, which in this particular study was oaten or lucerne hay, is its level of voluntary intake, which depends in part on palatability. Palatability is an integrative term (Provenza 1995); to provide a quantitative measure of the acceptability of a feedstuff, a „preference value’ can be obtained by describing the preference of one hay relative to an alternate hay also on offer. The Australian fodder industry is increasingly adopting more objective measures of hay quality to improve marketing opportunities, especially in the export industry, and to meet the demands and expectations of local and overseas purchasers of hay. Being able to efficiently and reliably predict the preference value of any particular hay would be beneficial to processors, exporters, users (purchasers) of hay, and possibly plant breeders, to make more informed decisions. This thesis describes a comprehensive analysis of the chemical and physical characteristics of a selected number of oaten and lucerne hays and their relationship to the acceptability or „preference value‟ of the hays for lactating Holstein Friesian cows and Thoroughbred horses. The project aimed to (i) quantify preference values for a large number of oaten hays with dairy cows and horses and a similarly large number of lucerne hays with horses only, and (ii) develop predictive equations for animal preferences based on the chemical and physical properties of the hays and the animal‟s short-term rate of consuming the hays. A total of approximately 8,500 preference tests were conducted with 85 oaten hays offered to lactating Holstein Friesian cows and Thoroughbred horses, and 70 lucerne hays offered to Thoroughbred horses. The hays were selected to cover a range of nutritive values. The intake rate and preferences for all the hays were quantified in a series of tests replicated over time and with different animals. Each „trial‟ hay was offered with four „standard‟ hays (in separate tests). A suite of chemical (nutritive value) traits and physical traits were quantified for the hays. An assessment of the „odour profile‟ was also included in the characterisation of the hays. The nutritive, physical and volatile traits were then related to hay preference values in multiple and simple linear regression models and equations generated to predict hay preference values. A prediction of hay preference made directly from the spectra obtained by near infrared reflectance spectroscopy (NIRS) was also developed. Overall, the average preference value of an oaten hay (i.e., that obtained using the comparisons with all four standard hays) could be predicted from a range of nutritive value traits, typically the contents of acid detergent fibre (ADF), hemicellulose, crude protein (CP) and water soluble carbohydrates (WSC) - with a correlation co-efficient of about 79% with cows and 61% for horses. Acid detergent fibre and CP contents tended to have the biggest influence on preference value for both cows and horses, followed by WSC and hemicellulose content. Examination of lucerne hays offered to horses increased the correlation co-efficient to 74%, with ADF, hemicellulose and CP each having a similar magnitude of effect. This means that these traits, with an appropriate weighting for each, could account for up to about three-quarters of the variation in hay preference values for cows and horses. The in vitro digestibility of dry matter (IVD) of oaten and lucerne hay was nearly as accurate in predicting preference values as using the four nutritive value traits of ADF, hemicellulose, CP and WSC, which was anticipated as digestibility is a function of these chemical traits. Hay physical traits could also be used to predict preference values, although not as accurately as the nutritive value traits or IVD. Of the physical traits, shear energy had the largest effect (co-efficient approximately -1.15 compared to an average of -0.35 for the other traits in the equation). The use of NIRS to directly predict preference values was also encouraging, with the best calibration model yielding a correlation co-efficient of 61 to 81%, depending on the hay (oaten or lucerne) and the animal (cows or horses). Of the total 120 volatile compounds isolated from the oaten hays, six had some relationship with cow preferences and there were also six oaten hay volatiles related to horse preferences. Of the oaten hay volatiles significantly related to preference, four were positively related to cow preference whilst two were negatively related to cow preference. Similarly there were four volatiles positively related to horse preference and two consistently negatively related. Of the six oaten hay volatiles found to influence the preferences of dairy cows and horses, four were common to both species. Of the total of 147 volatile compounds isolated from the lucerne hays, 15 had some relationship with horse preference, with nine positively related to horse preference and six negatively related. The data reported here suggest that a predicted preference value for a hay can be generated using commonly measured quality traits, which would improve a buyer‟s confidence of animals responding favourably when first offered the hay. More specifically, for an average preference value, the most reliable prediction equation was based on nutritive value traits: ADF, hemicellulose, crude protein and water soluble carbohydrates solely. Alternatively a combination of nutritive and physical traits can be used to predict preference: in vitro digestibility, crude protein, water soluble carbohydrates and shear energy. The visible and near infrared spectra obtained by NIRS was also a promising method of prediction, and given the speed and affordability of NIRS, this technology could be further refined and used for routine measurement of predicted hay preference values. The volatile compounds shown to influence preference value of the hay should be identified and further research undertaken to investigate novel approaches to manipulate the preference of hays. Caution should be taken when attempting to use these prediction equations on a single specific hay in a single specific situation. The predictions and relationships investigated in this thesis are based on populations of hays and preferences of a group of animals and therefore some care should be taken when applying them to a particular situation with difference circumstances. Other factors that can influence feed preferences include an animal‟s feeding experiences, basal diet and diet history and various environmental factors unaccounted for here. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1349258 / Thesis (Ph.D.) - University of Adelaide, School of Agriculture, Food and Wine, 2008

Thesis (MScAgric (Animal Sciences))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: The purpose of this study was to evaluate the production of slaughter ostriches in a grazing environment at different levels of supplementary feed. Two grazing trials were conducted. In the first trial, one group of finisher ostriches (six months old) was put into a feedlot and received a complete finisher diet. The other four groups were allowed to graze lucerne pasture (stocking rate of 15 birds/ha) with 1500, 1000, 500, and 0g supplementary feed/bird/day. Pasture production and intake were measured. There was no difference (P >0.05) between the end mean live weights of the feedlot ostriches and those two grazing groups receiving 1500 or 1000g supplementation. The average daily gain (ADG) of the group receiving 1000g supplementation was lower (P <0.05) than the ADG of the group receiving 1500g supplementation, but all three groups reached a mean target slaughter weight of 95kg within the 154 days of the study. Therefore, pastures together with the correct supplementation (at least 1000g/bird/day) can replace complete feeds in the finishing phase of slaughter ostriches and can play an important role in the production of these birds. For lucerne intake, a quadratic relationship (P <0.01) was found between pasture dry matter (DM) intake (g/bird/day) and supplementary feed intake (g/bird/day). The maximum lucerne intake level (1692.8g/bird/day) was achieved at 619.6g supplementary feed/bird/day. In the second grazing trial, finisher ostriches were allowed to graze lucerne pastures at two different stocking rates (10 and 15 birds/ha) while receiving either 0 or 800g supplementary feed/bird/day. Ostriches receiving supplementation had higher (P <0.05) mean end live weights than ostriches receiving no supplementation. Ostriches receiving supplementation reached a mean target slaughter weight of 95kg within the timespan of the trial, but ostriches receiving no supplementation did not. Stocking rate had no influence on mean end live weight of the birds. An interaction (P <0.05) was found between the level of supplementation and stocking rate for ADG of the birds. Stocking rate influenced ADG only for birds receiving no supplementation. As stocking rate increased, ADG of birds receiving no supplementation declined. Results of the pasture data indicated an increasing level of replacement of grazed lucerne DM by supplementary feed as the trial progressed and this was more pronounced at the higher stocking rate of 15 birds per hectare. A high stocking rate seems to have had a gradual depressing effect on lucerne DM production, while the less severe levels of defoliation at a lower stocking rate promoted lucerne DM production. A digestibility trial was conducted with mature ostriches (12 months old) to investigate the effect of supplementation on intake and digestibility of nutrients, as well as to investigate the substitution effect that ostriches may display when they receive supplementary feed in addition to grazing. The same dietary treatments as in the first grazing study were given to ostriches while they were kept in metabolism crates. These diets were also fed to 20-week old roosters to obtain energy values for these diets for roosters. These energy values would be used to predict ostrich energy values for the same diets by means of a regression equation. For the roosters, each diet treatment was mixed with 50% maize to prevent digestive disorders and ensure maximum feed intake. Ostriches started to substitute supplementary feed for pasture when supplementation was supplied at levels higher than 62% (i.e. 1000g supplementary feed/bird/day) of total feed intake. For each increase of 100g in supplementary feed intake, pasture was replaced at a rate of 4.9%. Higher (P <0.05) total feed intakes were reached by ostriches if they grazed lucerne pastures and received supplementation than if they grazed pasture alone. Pasture grazing alone had lower (P <0.05) dry matter digestibility (DMD) and apparent metabolizable energy (AME) values for both ostriches and roosters than if pastures were supplied with a supplement. A significant stepwise regression could not be computed for the prediction of ostrich AME values from rooster AME values. The economics of different feeding systems (extensive versus intensive) were evaluated with an economic analysis, which was based on the same materials and methods and results of the first grazing trial. A margin above feed cost (MAFC) analysis was performed to evaluate the economic viability of the different feeding systems. The present value (PV) of the MAFC for the pasture-based system with 1000g/bird/day supplementation was only 8.3% lower than that of the feedlot system over a period of six years, while the PV of the cost of the same pasture-based system was 78.4% lower than that of the feedlot system. Birds finished on lucerne pasture with 1000g supplementation led to a saving of 57% in feeding costs if compared to a feedlot system. A sensitivity analysis of the MAFC revealed that the pasture-based system was less sensitive to changes in feeding costs than the feedlot system. Therefore, the unique circumstances of each ostrich producer will play a role in the decision whether to raise ostriches in a feedlot or on pastures. / AFRIKAANSE OPSOMMING: Die produksie van slagvolstruise in ‘n ekstensiewe weidingsstelsel met verskillende vlakke van aanvullende voeding is gedurende hierdie studie ge-evalueer. Twee weidingsstudies is uitgevoer. In die eerste studie is een groep afrondingsvolstruise (ses maande oud) in ‘n voerkraal geplaas en ‘n volledige afrondingsdieet gevoer. Die ander vier groepe is op besproeide lusernweiding geplaas (teen ‘n weidigtheid van 15 voëls/ha) en het onderskeidelik 1500, 1000, 500 en 0g aanvullende voeding/voël/dag ontvang. Weidingproduksie en -inname is gemeet. Daar was geen verskil (P >0.05) tussen die eindgewigte van die voerkraal volstruise en dié van die weidende voëls wat onderskeidelik 1500 en 1000g aanvullende voeding ontvang het nie. Die gemiddelde daaglikse toename (GDT) van die groep weidende voëls wat 1000g aanvullende voeding ontvang het was laer (P <0.05) as die GDT van die groep weidende voëls wat 1500g aanvullende voeding ontvang het, maar al drie hierdie groepe het ‘n gemiddelde teiken slaggewig van 95kg bereik binne die 154 dae van die studie. Weiding, tesame met die korrekte aanvullende voeding (van ten minste 1000g/voël/dag) kan volvoer rantsoene in die afrondingsfase van slagvolstruise vervang en kan dus ‘n belangrike rol speel ten opsigte van die produksie van hierdie voëls. Vir lusern inname is ‘n kwadratiese passing (P <0.01) tussen weiding droë materiaal (DM) inname (g/voël/dag) en aanvullende voeding inname (g/voël/dag) gevind. Die maksimum lusern inname (1692.8g/voël/dag) is bereik wanneer voëls 619.6g aanvullende voeding/voël/dag ingeneem het. In die tweede weidingsstudie, is afrondingsvolstruise (6 maande oud) toegelaat om lusern te bewei teen twee verskillende weidigthede (10 en 15 voëls/ha) en het ook 0 of 800g aanvullende voeding/voël/dag ontvang. Volstruise wat aanvullende voeding ontvang het, het hoër (P <0.05) gemiddelde eindgewigte bereik as volstruise wat geen aanvullende voeding ontvang het nie. Volstruise wat aanvullende voeding ontvang het, het ook die teiken slaggewig van 95kg bereik binne die tydsduur van die studie, terwyl die volstruise wat geen aanvullende voeding ontvang het nie, nie daarin kon slag nie. Weidigtheid het nie ‘n invloed (P >0.05) gehad op die eindgewigte van die voëls nie, maar ‘n interaksie (P <0.05) is gevind tussen vlak van aanvullende voeding en weidigtheid wat GDT van die voëls betref. Weidigtheid het GDT beïnvloed slegs vir volstruise wat geen aanvullende voeding ontvang het nie. Soos die weidigtheid van die voëls wat geen aanvullende voeding ontvang het nie, toegeneem het, het die GDT van hierdie voëls afgeneem. Ontleding van die weidingsdata het ‘n toenemende vlak van verplasing van die weiding met aanvullende voeding getoon soos die studie gevorder het en dit was meer merkbaar by die hoër weidigtheid. Die hoër weidighteid het ook gelei tot ‘n geleidelike afname in lusern DM produksie, terwyl die minder aggressiewe vlakke van ontblaring by die laer weidigtheid lusern DM produksie bevorder het. ‘n Verteringsstudie is gedoen met volwasse volstruise (12 maande oud) om die invloed van aanvullende voeding op inname en verteerbaarheid van nutriente te toets, asook om die substitusie effek wat volstruise mag toon wanneer hulle aanvullende voeding ontvang op weiding, te ondersoek. Dieselfde dieet behandelings as in die eerste weidingsstudie is vir die volstruise gegee terwyl hulle in metabolisme kratte aangehou is. Hierdie diëte is ook aan 20-week oue hane gevoer om die energie waardes van die diete vir hane te verkry. Hierdie energiewaardes sal dan gebruik word om volstruis energiewaardes te voorspel vir dieselfde diëte met behulp van ‘n regressie vergelyking. Vir die hane is elke dieet gemeng met 50% mielies om inname te handhaaf en spysverteringsstoornisse te voorkom. Volstruise het weiding begin verplaas met aanvullende voeding sodra die vlak van aanvullende voeding hoër as 62% (d.i. meer as 1000g aanvullende voeding/voël/dag) van die totale inname van die voëls was. Vir elke 100g toename in aanvullende voeding, word weiding verplaas teen ‘n tempo van 4.9%. Weiding, tesame met aanvullende voeding, het gelei tot hoër totale droë material (DM) voerinnames by volstruise as wanneer weiding alleen beskikbaar was. Wanneer weiding alleen voorsien was, was daar laer (P <0.05) verteerbaarhede van DM en waarskynlike metaboliseerbare energie (WME) waardes vir beide volstruise en hane as wanneer die weiding voorsien word met aanvullende voeding. Geen betekenisvolle stapsgewyse regressie kon gevind word om volstruis energie waardes uit hoender energie waardes te voorspel nie. Die ekonomie van verskillende sisteme (ekstensief versus intensief) is in hierdie studie vergelyk en is gebaseer op dieselfde materiaal en metodes en resultate van die eerste weidingsstudie. ‘n Marge bo voerkoste analise is gebruik om die ekonomiese lewensvatbaarheid van die sisteme met mekaar te vergelyk. Die huidige waarde van die marge bo voerkoste van die weidingssisteem waar 1000g aanvullende voeding gevoer word was 8.3% laer as dié van die voerkraal sisteem oor ‘n periode van ses jaar. Daarteenoor was die huidige waarde van die koste van dieselfde weidingssisteem 78.4% laer as dié van die voerkraal sisteem. Die weidingssisteem waar volstruise 1000g aanvullende voeding ontvang het, het ‘n besparing van 57% in voerkoste getoon wanneer dit met die voerkraal volstruise vergelyk is. ‘n Sensitiwiteitsanalise van die marge bo voerkoste het getoon dat die weidingssisteem minder sensitief is vir wisselende voerkoste as die voerkraal sisteem. Die unieke omstandighede van elke produsent sal ‘n rol speel in sy keuse om volstruise op weiding of in ‘n voerkraal af te rond.

[Truncated abstract] In southern Western Australia the replacement of deep-rooted native vegetation with annual species has resulted in rising water tables and increased salinity due to insufficient water use. The area has a Mediterranean-type climate where rainfall during summer is generally low but variable resulting in limited plant growth. However, if rainfall does occur it potentially can contribute to to the increased water excess or drainage by increasing the soil water content before the main drainage period in winter. The first study investigated factors controlling soil water content changes during the fallow (December to May) in annual farming systems. This was achieved by examining variation in available soil water storage to a depth of 1.0-1.5 m at three sites within 13 seasons. Reasons for the variation were examined using the Agricultural Production Systems Simulator (APSIM). This study also investigated the relationship between soil water content at the end of the fallow period (1 May) and the amount of drainage below 2.5 m by using APSIM coupled to historical weather records at three locations. At the end of the fallow a mean of 24 mm (or 25%) of rainfall during the fallow was retained in the soil. Losses of soil water during the fallow were due to evaporation (mean of 60 mm), transpiration from plant cover (mean of 12 mm) and drainage below the root zone and run off (combined mean of 13 mm). Soil water accumulation during the fallow period had a significant impact on simulated drainage under wheat in the following growing season. Every 1 mm increase in soil wetness at the end of the fallow resulted in a 0.7-1 mm increase in simulated drainage during the growing season. ... Variation in the water excess due to variation in rainfall was greater than the reduction in water excess due to lucerne. This makes the decisions about when to grow lucerne to reduce water excess difficult if livestock enterprises are less profitable than cropping enterprises. The findings of this PhD indicate that lucerne does have a place in Mediterranean-type environments because of its greater water use than current farming practices. However, its use needs to be strategic and the strategy will vary from region to region. For example, in the low rainfall region lucerne sowings need to be matched with high soil water contents and phase length will generally be short (2-3 years). In comparison at high rainfall regions lucerne will need to be grown for longer or combined with other strategies to increase water use.