The goal of this project is extraction of chitosan optimally by surveys of temperature changesalong with 1% Sulfuric acid utilization. Microbial chitosan is isolated as a bio-componentfrom cell wall of two kinds of Zygomycetes by some extraction methods. This projectcompares ability of two type Fungi (R. pulusilus and M.indicus) from Zygomycets forproduction of chitosan.To extract of chitosan is a combinational method with 40 %( w/w) Hydroxyl sodium for celldisruption and diluted Sulfuric acid (1% w/v) for chitosan extraction from cell wall as majorchemical components. 40% NaOH is used to get different degrees of deacetylation (DD) fromchitin for chitosan. In addition, it is examined 1% Sulfuric acid in a combination oftemperature factor changes. It is needed dialysis for chitosan purification from bondedphosphate groups. Standard curves of acetic acid experiences for DD and water phosphatedetermination were accomplished.It has resulted if degree of deacetylated chitin is about 50%; it has an average lost more than50% in 1% (v/v) Sulfuric acid, hence less recovery as a no privilege that it can be relapsed byacetone in chitosan solution. Factor of temperate in same times shows important effect onextraction yield of chitosan by 1% Sulfuric acid. Extracted chitosan in 120℃ has DD about50%. Absolutely, its solubility will be more and it needs to an intricate solution for separationof chitosan from phosphate bonds as a major impurity by dialysis, but in 90℃, DD of chitosanis more with less solubility in water.Between two Fungi, in experienced temperatures, hence, R. pulusilus has more recovery about0.87/AIM (g/g) in 90℃, which have more much DD than 50%, and M.indicus has 0.79/AIM(g/g) in 120℃ that it has DD about 50%.