It has been proposed that geochemical and biomolecular palaeontological information can be obtained from biomineral associatedli pids. The location of lipidic material within the inorganic structureo f molluscan shells has previously been unknown, with important implications for long term survival of lipids and post-depositional contamination from the environment. Discrete experimental stages have been investigated and the different mechanical and chemical methods combined for the removal of contaminating material prior to the release and analysis of surficial, intercrystalline and intracrystalline lipids. Three extraction protocols have been compared using Recent Patella vulgata shells. Sequential stages of cleaning and extraction treatments identify n-alkanes, cholesterol (free and bound) and bound fatty acids. The n-alkanes are indigenous to the shell, but laboratory contamination can be significant, and highlights the need for experimental blanks. Bound fatty acids are extracted from intercrystalline and intracrystalline fractions. Cholesterol is extracted throughout the sequential methodology. The extraction of these compounds after extensive cleaning treatments illustrates the protective role of the inorganic biomineral. An experimental protocol for sequentially extracting protected lipids from the shells of Recent molluscs has been tested to distinguish the indigenous shell lipids from laboratory contamination and postdepositional ingress. The use of a calcium carbonate blank reveals the phthalate plasticisers extracted from the shells are due to laboratory contamination. Pristane, phytane and free fatty acids were rarely extracted which limits their use for interpretation. The n-alcohols, bound fatty acids, ß-hydroxy fatty acids, cholesterol and other steroids are extracted from the shells in higher yields than the calcium carbonate blanks and are considered indigenous to the shells. Multivariate statistical analysis is used to compare the distributions of bound fatty acids and steroids extracted from different shell locations with the reported fatty acids and steroids for the soft tissues of the same species. The reported values for the soft tissues were used to indicate the original shell lipid composition. The shells lack the unsaturated bound fatty acids reported in the soft tissues. The saturated bound fatty acids of Littorina littorea shells also differ in the carbon number distributions to the reported saturated fatty acids of the soft tissues. Surficial shell extracts are characterized by steroidal ketones, representing sterols which have been oxidised by the cleaning treatments used. The steroids from both intercrystalline and intracrystalline shell locations in Littorina littorea are most similar to the soft tissues. However, the intercrystalline steroids are different to the intracrystalline steroids which may indicate a different original composition. Potential Class level phylogenetic differences between the shells of Recent molluscs are revealed by their steroidal and bound saturated fatty acid compositions. The bivalves (n=3) have bound saturated fatty acids with a carbon number maximum of C16 whilst the gastropods (n=8) have a maximum of C16 or C18 and exhibit higher yields. ß-hydroxy fatty acids may indicate phylogenetic differences below the Class level for the Gastropoda. Principal component statistical analysis of the shell steroidal composition indicates differences at the Class level. Steroidal markers indicating the dietary intake have been found in the shells. The application of a methodology for the sequential extraction of lipids from molluscan shells has been used in a preliminary analysis of shell material for the presence of hydrocarbon pollutants. The shell nalkanes require comparison of carbon number distributions and yields with an experimental calcium carbonate blank to ensure indigeneity. Different n-alkane distributions within two Artica islandica shell samples are attributed to the different sampling locations. Differences between Patella vulgata and Littorina littorea shells from the same environment have also been observed, indicating different n-alkane uptake by different species. Polyaromatic hydrocarbons and sterane biomarkers reported to be present in the soft tissues of Patella vulgata exposed to the Braer oil spill have been searched for in the shells of an exposed sample. These compounds have not been detected. No increase in the shell n-alkane yields or similarity in carbon number distribution with the spilt oil is observed. This suggests no hydrocarbon incorporation or deputation pathway into the shell. Quaternary aged mollusc shells yield n-alkanes, n-alcohols, bound fatty acids and cholesterol. These have been extracted from both intercrystalline and intracrystal line locations within the shells. When compared with the extracts from Recent shells the yields of these lipids from fossil shells are significantly lower. The n-alkanes extracted from Quaternary shells are dominated by laboratory contamination, although some indigenous intracrystalline n-alkanes have been extracted. The bound fatty acids from intercrystalline sites within the fossils maintain their carbonn umber distribution but decreasein yields with increasinga ge; no diagenetic products were observed. The previously reported phylogenetic distinctions based on the bound fatty acids betweent he gastropodsa nd bivalves are maintainedf or fossils. However,t he information obtained from this analysis is limited by the small diversity of lipid distributions found in these fossil shells.

No description available.

This thesis combines detailed analyses of living plant communities with paleolimnological methods to develop the use of plant macrofossils as an effective tool to track hydroecological changes in ponds of the Slave River Delta (SRD), N.W.T. Several approaches were used to develop an understanding of the relationships between hydrolimnological conditions, living plant communities, and the composition of sedimentary macrofossil remains across spatial and temporal scales. A spatial survey approach was used to assess the relationships between the composition of plant macrofossil assemblages contained in the surface sediments and the hydrological and limnological conditions of 40 SRD basins that span a broad range of hydrological settings in the delta. Results show that there are strong relationships between the prevailing hydrolimnological conditions in the SRD and the composition of sedimentary macrofossil remains, and subsequently indicator macrofossil taxa were identified to distinguish between sites with high river influence (flood-dominated sites: ostracode shells, Daphnia ephippia, Chara oospores; exchange-dominated sites: Myriophyllum winter-bud scales, Daphnia ephippia) and sites with low river influence (evaporation-dominated: Ceratophyllum leaves, Lemna leaves, Drepanocladus leaves). In light of the strong connection between spring flood events and the hydrolimnological conditions of SRD ponds, and growing concerns that the frequency of spring floods have declined in recent decades, paleolimnological investigations were initiated at a pond (SD2) adjacent to the Slave River to construct a record of flood events in the SRD. Prior to analysis of macrofossil assemblages from sediment cores, a detailed study of the living plant community was conducted at SD2, and results were compared to the distributions of surficial sedimentary plant macrofossil assemblages to assess how representative surficial sediment assemblages are of the living plant community. This study indicates distinct patch-scale (or quadrat-scale)similarity between the living vegetation and sedimentary remains in the central basin, as well as distinct similarity between the living aquatic macrophytes and sedimentary remains at a pond-scale, suggesting there is excellent potential to track changes in the composition and percent cover of aquatic macrophytes in pond sediment cores using plant macrofossil assemblages. Additionally, this study indicates that influence of long-distance transport of macrofossils during the 2005 flood event was minor at this pond, and may not be an important factor affecting paleolimnological reconstructions of plant communities. With contemporary studies as a framework, an ~90-year record of ice-jam flood frequency was reconstructed from a sediment core collected from pond SD2. Multi-proxy analyses indicated decadal-scale oscillations in flood frequency at this site, with at least three multi-year periods of low river influence. Beginning in ~1943, an 18-year period of particularly low river influence and greatly reduced water levels was indicated by abundant macrofossils of Sagittaria cuneata and represents the driest period over the past ~90-years. Similarities between the flood history of SD2 and upstream sites in the Peace-Athabasca Delta suggest that spring discharge generated from headwaters and major contributing rivers plays a key role in the frequency and magnitude of spring flood events of both deltas.

This thesis combines detailed analyses of living plant communities with paleolimnological methods to develop the use of plant macrofossils as an effective tool to track hydroecological changes in ponds of the Slave River Delta (SRD), N.W.T. Several approaches were used to develop an understanding of the relationships between hydrolimnological conditions, living plant communities, and the composition of sedimentary macrofossil remains across spatial and temporal scales. A spatial survey approach was used to assess the relationships between the composition of plant macrofossil assemblages contained in the surface sediments and the hydrological and limnological conditions of 40 SRD basins that span a broad range of hydrological settings in the delta. Results show that there are strong relationships between the prevailing hydrolimnological conditions in the SRD and the composition of sedimentary macrofossil remains, and subsequently indicator macrofossil taxa were identified to distinguish between sites with high river influence (flood-dominated sites: ostracode shells, Daphnia ephippia, Chara oospores; exchange-dominated sites: Myriophyllum winter-bud scales, Daphnia ephippia) and sites with low river influence (evaporation-dominated: Ceratophyllum leaves, Lemna leaves, Drepanocladus leaves). In light of the strong connection between spring flood events and the hydrolimnological conditions of SRD ponds, and growing concerns that the frequency of spring floods have declined in recent decades, paleolimnological investigations were initiated at a pond (SD2) adjacent to the Slave River to construct a record of flood events in the SRD. Prior to analysis of macrofossil assemblages from sediment cores, a detailed study of the living plant community was conducted at SD2, and results were compared to the distributions of surficial sedimentary plant macrofossil assemblages to assess how representative surficial sediment assemblages are of the living plant community. This study indicates distinct patch-scale (or quadrat-scale)similarity between the living vegetation and sedimentary remains in the central basin, as well as distinct similarity between the living aquatic macrophytes and sedimentary remains at a pond-scale, suggesting there is excellent potential to track changes in the composition and percent cover of aquatic macrophytes in pond sediment cores using plant macrofossil assemblages. Additionally, this study indicates that influence of long-distance transport of macrofossils during the 2005 flood event was minor at this pond, and may not be an important factor affecting paleolimnological reconstructions of plant communities. With contemporary studies as a framework, an ~90-year record of ice-jam flood frequency was reconstructed from a sediment core collected from pond SD2. Multi-proxy analyses indicated decadal-scale oscillations in flood frequency at this site, with at least three multi-year periods of low river influence. Beginning in ~1943, an 18-year period of particularly low river influence and greatly reduced water levels was indicated by abundant macrofossils of Sagittaria cuneata and represents the driest period over the past ~90-years. Similarities between the flood history of SD2 and upstream sites in the Peace-Athabasca Delta suggest that spring discharge generated from headwaters and major contributing rivers plays a key role in the frequency and magnitude of spring flood events of both deltas.

Studies of vegetation history bring a new incentive to our understanding of plant survival and migration in arctic environments. For decades, environmental research was based on palynological data and these studies created a notion that tree species such as larch (Larix sibirica) and Scots pine (Pinus sylvestris) did not grow in northern Scandinavia at the end of Weichselian glaciation. However, findings of macro- and megafossils of these trees dating back to glacial times has been reported in the Swedish mountain range, questioning this view of a late arrival of these trees in Scandinavia. The apparent contrasting views on the composition of the first plants arriving to Scandinavia create uncertainties about the bioclimatic conditions prevailing at the end of the Weichselian glaciation. To improve our understanding about the first vegetation arriving to Scandinavia I probed the macrofossil composition of two novel sedimentary records from northern Sweden. Twelve sediment cores from material underlying Holocene peat deposits were used as archives of early Holocene plants. In these records, I found: I) larch needles dating back to 4.6 and 4.1 calibrated thousand years (cal. kyr) BP; II) pine macrofossils dating back to 9.5 and 8.7 cal. kyr BP; III) fossils from dwarf shrubs (willow and heather) dating back to 9.9 cal. kyr BP; and IV) a birch fossil dating back to 9.5 cal. kyr BP. Also found in the same depth was fragment of a spruce cone. Based on my findings, I concluded that the landscape behind the retreating Weichselian ice-sheet was surpassingly colonised by pine and larch trees, a forest that has no contemporary analogue in Scandinavia. It seems as if this early forest also contained spruce, which is enigmatic as the main spruce invasion is expected to occur across the region during the next millennia. Finally, there is an instigation for future discussion on how our present knowledge of plant behaviour in changing conditions can help minimise the impacts of ever-expanding climate change.

The end–Triassic mass extinction event (ca. 201 Myr ago) has received particular attention over recent decades since Sepkoski (1981) classified it as one of the ‘‘big five’’ biotic crises in Earth's history. In the geological record of Greenland and Sweden, 80% of the species of terrestrial plants disappeared at this boundary. In the last two centuries, Triassic–Jurassic plant remains from Skåne, southern Sweden, have been collected, curated, and studied. However, the paleoflora from the lowermost part of the Helsingborg Member (Lower Jurassic: Hettangian) is poorly understood. Here, a taxonomic study is presented of two novel plant assemblages collected from the Boserup beds (basal Hettangian) in NorraAlbert Quarry, Skåne. The exposures in Skåne are among the few localities in the world that record the terrestrial ecosystem aftermath of the end–Triassic extinction event. Plant macrofossils were studied using macrophotography and fluorescence microscopy. The flora is composed of sphenophytes(Neocalamites), ferns (Cladophlebis, Eboracia), ginkgophytes (Czekanowskia, Pseudotorellia,Ginkgoites), and conifers (Pityophyllum, Brachyphyllum). These earliest Jurassic assemblages were deposited in floodplain environments and revealed a relatively low diversity of flora in the aftermath of the end-Triassic extinction but a fast recovery later. Additionally, the flora was compared with the relative lowermost Jurassic beds in East Greenland, Poland, and Germany, disclosing that ginkgophytes were widely distributed across the northern region of Pangea.

En omfattande multiproxyanalys har utförts på provtagningar från två vattenhål belägna i Skåneregionen i Sverige. Denna mångsidiga analys involverade flera aspekter, inklusive markkemi, entomologi, mikrofossiler och makrofossiler. Resultaten från markkemianalysen avslöjade betydande skillnader mellan de två vattenhålen. Vattenhål 1 uppvisade en högre halt av organiskt material, vilket tyder på högre glödförluster och högre värden av maximal magnetisk susceptibilitet (MS550) och totalfosfat (CitPOI). För att ytterligare karakterisera egenskaperna hos vattenhålen användes även analyserna infrarödspektroskopi (NiR) och röntgenfluorescens (XRF).   Vattenhål 2 visade tecken på att ha fyllts med bosättningsavfall från platsen. Detta stöddes av markkemiresultaten som visade en lägre halt av organiskt material samt närvaron av antropogena material, såsom förkolnade frön, träkol, bränd lera, ben och keramikfragment.   Makrofossilanalysen avslöjade intressanta fynd. I vattenhål 1 hade växter som etternässla (Urtica urens) och kråkklöver (Comarum palustre) deponerats, tillsammans med enstaka exemplar av Thrips major Uzel. I kontrast bestod de förkolnade fröna från vattenhål 2 huvudsakligen av ogräs, inklusive svinmålla (Chenopodium album) och nattskatta (Solanum nigrum). Det fanns även spår av sädeskorn som korn (Hordeum vulgare), samt oidentifierade fragment av sädeskorn. Resultaten från makrofossilanalysen indikerade att materialet från vattenhål 1 härstammar troligtvis från forntida mänskliga aktiviteter, som odling.   Mikrofossilanalysen visade att det inte fanns några kiselalger (diatoméer) i något av vattenhålen. Sammantaget antyder resultaten från multiproxyanalysen att vattenhål 1 hade genomgått minst tre olika faser i sin historia, medan vattenhål 2 hade fyllts med jord och material från en bosättningsliknande plats, vilket gjorde det svårt att entydigt tolka själva vattenhålets historia och funktion.

Two peat cores from two mires with different characteristics, but both containingpermafrost features and located in the eastern part of the Tavvavuoma mire complex innorthernmost Sweden, were analysed for macrofossils and geochemical properties. Local vegetationsequences and changes in geochemical properties of peat were used to reconstruct development ofthe studied mires during the Holocene. The study includes measurements of water/ice content, bulkdensity, loss-on-ignition and C/N ratio. Radiocarbon dates for peatland inception and permafrostaggradation are available. The main purpose of the study is to verify permafrost history in thepeatlands. The results of the macrofossil analysis and values of C/N ratio indicate nutrient poor tointermediate fen environments in both studied mires until recently. Signs of permafrost upheavalwhich caused formation of xerophilic peat can be proved only since late 1950’s. The study resultscorroborate with other studies from Northern Fennoscandia and infer peatland initiation soon afterthe deglaciation of the area and permafrost-free conditions throughout entire Holocene untilrecently.

The Danubian Plain is nothern part of the Pannonian Plain and in terms of palaeobotanical research it is an entirely unexplored area. However, this region is assumed to contain cryptic glacial refugia and migration routes of thermophilous biota northward after the Last Glacial Maximum. The late-glacial and Holocene natural long-term succession and temporal changes in the composition of plant communities of lowland lakes in the Slovakia is unknown. Šúr, a National Nature Reserve is a former lake, presently overgrown by alder carr forest. Successional development since the late-glacial was studied along two profiles. Macrofossil analysis was carried out on lake sediments extending to the Middle Pleniglacial (OIS 3). Using knowledge of the modern ecological requirements of taxa, past environmental conditions were reconstructed. The historical biogeography of selected plant species in Europe during the late- glacial and Holocene is also discussed. During the Middle Pleniglacial Myriophyllum spicatum and Chara sp. grew in the lake. During the late-glacial shallow basin contained a calcareous, meso- to eutrophic lake with Potamogeton filiformis, P. praelongus, Ranunculus subgen. Batrachium etc. During the early Holocene these elements disappeared and thermophilous plants immigrated, such as the...

Heteromorph ammonites and dinoflagellate cysts from the Upper Cretaceous Northumberland Formation on Hornby Island, British Columbia, Canada are examined. The collection and preparation of new material has enabled the recognition of eleven species of which only three have been reported from the locality. Of these taxa represented from three heteromorph ammonite families in the study area, five are new occurrences and three are new to science. This expansion of the Hornby Island ammonite fauna is presented alongside a pioneering taxonomic survey of dinoflagellate cysts from the same rocks. Together, these macro- and microfossils reinforce a late Campanian age for the Northumberland Formation with the upper extent of the section approaching the Campanian-Maastrichtian boundary (CMB) interval. The palaeoecology and evolutionary relationships of these heteromorph ammonoids are considered with new insights into their ontogenetic development and neritic palaeoenvironmental circumstances. The dinoflagellate cysts and associated terrestrial palynomorphs have also allowed for enhanced palaeoenvironmental reconstruction and depositional setting inference. The scope of the studied material, and the presence of key index taxa, enables refined biostratigraphy and a stronger basis for correlation of the Hornby Island succession with neighboring coeval biotic provinces. / Graduate / 2018-08-10