There is strong evidence that the skeletal muscles of pinnipeds are adapted for an aerobic, lipid-based metabolism under the hypoxic conditions associated with breath-hold diving. However, regional variations in mitochondrial density are unknown, and the few fiber typing studies performed on pinniped skeletal muscles are not consistent with an aerobic physiological profile. Thus, the objectives of this study were to (1) reexamine the fiber type distribution throughout the primary locomotory muscles of the harbor seal, and (2) to better understand the density and distribution of mitochondria in the locomotory muscles. Multiple samples from transverse sections of the epaxial muscles and a single sample of the pectoralis muscle of wild harbor seals were analyzed using immunohistochemical fiber typing and electron microscopy. Fiber typing results indicated that harbor seal epaxial muscles are composed of 47.4% type I (slow twitch, oxidative) fibers and 52.8%, IIa (fast twitch, oxidative) fibers. No fast twitch, glycolytic (type IIb) fibers were detected in the epaxial muscles or the pectoralis muscle. Mean volume density of mitochondria [Vv(mt,f)] was 5.6%, which is elevated over what would be predicted for a terrestrial mammal of similar mass. The elevated Vv(mt,f) had a high proportion of intermyofibrillar mitochondria, a trait not normally found in the muscles of terrestrial mammals with elevated Vv(mt,f). These results provide further evidence that the elevated mitochondrial volume density in pinniped muscle decreases the oxygen diffusion distance between myoglobin and mitochondria to facilitate aerobic respiration in working muscles. In addition, analyses of heterogeneity revealed that the regions of the epaxial muscles that were located deep within the muscle showed a significantly higher Vv(mt,f) relative to those regions that were superficially-located. In contrast, there was no significant heterogeneity of fiber type detected in either plane of the epaxial muscles. Thus, there was a fine-scale pattern of spatial heterogeneity of Vv(mt,f) within the epaxial muscles that does not manifest in fiber type distribution, indicating that the fibers have similar oxidative capacities.

Japanese knotweed is an invasive plant that grows in disturbed sites across Nova Scotia. This study recorded an average spring growth rate of 6cm per day until reaching a canopy height exceeding 2m in June. Knotweed stands contained on average 17 stems and 8.0kg of fresh biomass per m2. Leaf cover was significantly greater in knotweed patches versus grass and shrub habitats in riparian ecosystems. Plant diversity in knotweed patches was nil, yet invertebrate diversity and abundances were similar across habitats. Small mammal tracks were more abundant in knotweed than shrub patches, but not as much as grass plots. Two herbicides were applied at four different dates in 2011. The following year, Aminopyralid was ineffective while Imazapyr treatments successfully reduced knotweed biomass, density, height, and leaf cover. Imazapyr application is recommended at full growth (June) or flowering (August). This project provides new information on an invasive weed in eastern Canada.

The demand by ecotourism for easily accessible wildlife encounters has increased the need for regulations to minimise negative effects of tourism on towards marine mammals. High levels of human interaction could have serious consequences for recovering populations of New Zealand fur seals (Arctocephalus forsteri). By monitoring behavioural shifts in reactions to human disturbance, the aim of this study was to determine how disturbance by tourism is affecting the behaviour of the New Zealand fur seals. Fur seal breeding colonies, haul-outs, and a pup nursery were studied on the South Island to determine the level of disturbance. Data collected in this study can be used towards improving monitoring regimes to mitigate negative effects of anthropogenic disturbance. I first used behavioural observations to assess a seal’s behaviour in response to different types of tourist activities. Next, I examined changes in New Zealand fur seal behaviour as a result of visits to colonies by tourist boats. To quantify the response of fur seals to tourist boats, experimental boat approaches were conducted using a before, during, and after instantaneous scan sampling method at two breeding colonies (one with high vessel traffic and one with none). Impact of noise was also investigated using a loud speaker to mimic local harbour tours. Lastly, behavioural observations on seal pups at a nursery were conducted comparing pup behaviour in the presence and absence of tourism along with variable intensities of tourist behaviour. My observations suggest that seal behaviour was significantly different between sites with and without tourist visits. The type of tourism had a significant effect on the behavioural state of seals, with animals more active when there were people walking in the colonies. There were also signs of habituation in some of the study colonies. Since each colony varied in the type of tourism it experienced, it is possible that it is not only the level of tourism that is important but also the type of tourism that has a significant role in eliciting short-term behavioural shifts. Observations from a tour boat revealed an increase in the percentage of seals reacting when vessels were close to the shore. This distance effect was overridden and reactions were greater, however, when tours included commentary via an external speaker. The effects of both distance and noises were significantly different between colonies with high and low levels of tourist visits. Due to the overlap of peak tourist visits with fur seal breeding season, these animals are at their most vulnerable when companies are in peak operation. Finally, as with adults, pup behaviour was also significantly affected by tourism presence. Periods of inactivity and awareness increased in the presence of tourists, which is indicative of disruption of “play” and movement towards more vigilant behaviour. Despite the significant effects of tourist visits I found in this study, there was large variation in the degree of responses in different populations of the fur seal. This variation is thought to be due, in part, by the level of desensitisation, especially at locations with high or continual tourism pressures. This study also provides evidence that fur seal pups subject to human disturbance will alter their behaviour, shifting from active (when people are not around) to inactive and more aware (with increased disturbance). The results presented suggest such visits are not without consequences and that animals can be disturbed by human interactions. Therefore, measures should be taken at all seal colonies used in tourism ventures to mitigate any negative long-term effect on the fur seal populations.

In arid landscapes limited resources are concentrated into fertile patches that allow greater productivity than would otherwise be expected. Fertile patches are created and maintained by processes that modify soil topography and interrupt the flow of resources across the landscape. These processes may be abiotic or biotic in origin. Species that modify, maintain or create habitat have been termed ecosystem engineers. One group of soil disturbing ecosystem engineers creates fertile patches by modifying soil microtopography through foraging for food and creating habitat. This thesis examines the effects of soil foraging animals on resource concentration in arid environments and how effects are moderated by species and landscape. Chapter 1 provides an overview of the importance of fertile patches in arid systems and describes previous research on the effects of ecosystem engineers in arid environments. Chapter 2 examines how fertile patch creation by a well-studied arid zone engineer, the ant, varies between ant species and landforms, demonstrating that while we can generalise about the effects of ant nests on water flow and nutrient levels, differences in soil type, nest density and ant species across sites are likely to moderate these effects. Chapters 3, 4 and 5 compare the engineering effects of reintroduced native species, the Greater bilby (Macrotis lagotis) and Burrowing bettong (Bettongia lesueur) with the Sand goanna (Varanus gouldii) and the invasive European rabbit (Oryctolagus cuniculus) across three Australian landforms, revealing differences between reintroduced and invasive engineers at the both patch and landscape scales. At the landscape scale, differences in pit densities and resource concentration in foraging pits between landforms suggest that fertile patch development is not uniform. Chapter 6 examines whether the structure of the pit or the presence of litter per se explains observed increases in plant germination in foraging pits, and compares temperature and soil moisture conditions in bilby and bettong pits to the soil surface. Chapter 7 is a review that builds upon previous chapters, published and unpublished literature of the potential for reintroductions of ecosystem engineers to restore ecosystem function in degraded environments. Chapter 8 summarises the research presented in this thesis and its implications, and suggests directions for future work.

Many species have suffered severe range contractions as a result of human impacts, and require careful management if they are to persist. An understanding of the ecology, population structure and mating system is important for the conservation of these endangered species. Translocation has become a widely used tool in the conservation of threatened species; however, without proper planning and monitoring, many programmes end in failure. The bridled nailtail wallaby is one of the most endangered macropods in Australia, having suffered an extensive range contraction from the semi-arid region of eastern Australia to a single remnant population of ~ 500 animals at Taunton National Park in Queensland. In 1996, a translocated population of bridled nailtail wallabies was established at Idalia National Park, on the western edge of the former range of this species. The aims of this study were to: 1) determine the factors underlying variation in reproductive success among animals released at Idalia; 2) investigate the effects of a range decline on the genetic variation and population structure of the remnant population at Taunton; 3) assess the impact of captive breeding and translocation on the genetic diversity and structure of the established population at Idalia; and 4) investigate male and female mating strategies in this species. Chapter two investigated reproductive success among males released at Idalia National Park. The bridled nailtail wallaby is a sexually dimorphic, polygynous species and there is a large amount of variation in male reproductive success. Animals of different origin were released, including animals bred in captivity off the park, animals bred on-site in enclosures, and wild-caught animals from the remnant population that were moved directly to Idalia. There was significant variation in reproductive success among males released, but the origin of those animals did not explain this variation. Instead, large males were the most reproductively successful regardless of origin. Survival probability and release location were also important factors. This study highlighted the importance of considering mating system when choosing animals for translocation and suggests that, for polygynous species, a greater proportion of females and males of high breeding potential should be released. In Chapter three I investigated the genetic diversity and fine-scale genetic structure of the remnant population at Taunton. This last remaining population has been isolated for at least 60 years, corresponding to 30 generations for this species. Animals are patchily distributed in assemblages of different size that cover ~ 10% of suitable habitat on the park. Genetic diversity was surprisingly high within the population remnant and this species showed one of the highest levels of heterozygosity and allelic diversity of any macropod. The three sub-populations of animals sampled over 10 km were significantly differentiated, and fine-scale spatial genetic structure was also observed within a continuous sub-population of animals covering just 750 ha. Females were more related to each other than expected from random, whereas males were less related to each other than expected. Fine-scale genetic structure was observed among females but not males, providing evidence for female philopatry and male-biased dispersal. I suggest that fine-scale population structure and restricted gene flow among females but not males may maintain genetic diversity in this remnant population. Chapter four assessed the effect of translocation of animals of different origin on genetic diversity in bridled nailtail wallabies. Both wild-caught and captive-bred animals were initially released, but subsequent releases consisted of captive-bred animals only. In this study, I compared genetic diversity of released animals to animals sampled in the remnant population. Heterozygosity did not differ between the remnant and translocated population, however allelic diversity was significantly reduced among all groups released. Animals bred in captivity were significantly differentiated from the source population, whereas animals moved directly from Taunton were not. Releasing more captive-bred animals into the population after the initial release resulted in a decline in overall heterozygosity and allelic diversity. This study highlights the importance of sourcing animals directly from remnant populations in order to maintain genetic diversity and minimize genetic drift between source and recipient populations. The bridled nailtail wallaby has a polygynous mating system and reproductive success is skewed toward large males. However unlike most polygynous, sexually dimorphic macropods, the bridled nailtail wallaby is highly solitary; animals only associate to breed and males do not form stable dominance hierarchies. In Chapter five I investigated behavioural strategies used by males to secure matings, and mate choice among females. I found that males of different size adopted different mating strategies. Large males spent more time guarding females against other males and maintaining contact with them, whereas smaller males spent less time with guarded females in order to search for other females in oestrus. Females also preferred larger males and were more likely to engage in mate chases if there was a large number of males within the group.

Thesis (M.S.) --University of Montana, 2008. / Title from author supplied metadata. Description based on contents viewed on May 15, 2009. ETD number: etd-01142009-131434. Author supplied keywords: bear rubs ; grizzly bear ; mark-recapture ; noninvasive genetic sampling ; Northern Continental Divide Ecosystem ; Pradel model ; trend monitoring ; Ursus arctos. Includes bibliographical references.

Thesis (M.Phil)--Aberdeen University, 2009. / Title from web page (viewed on July 28, 2009). Includes bibliographical references.

Thesis (Ph. D.)--University of Washington, 2006. / Vita. Includes bibliographical references (leaves 102-116).

Thesis (M.S.)--Oregon State University, 2006. / Printout. Includes bibliographical references (leaves 75-83). Also available on the World Wide Web.

Thesis (Ph. D.)--University of Washington, 2006. / Vita. Includes bibliographical references (leaves 127-139).