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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
371

Quelques expériences sur l'évaporation de spray dense et la chimiotaxie de la mite / Some experiments on dense spray evaporation and moth chemotaxis

Rivas, Aloïs, de 12 July 2017 (has links)
Ce manuscrit présente des études expérimentales sur l'évaporation de gouttes et la chimiotaxie de la mite.Une première partie s'intéresse à l'évaporation de spray dense au moyen d'une nouvelle approche, par analogie avec le mélange scalaire. Dans cette limite de forte densité en gouttes, où il y a saturation en vapeur au sein des structures constituantes, l'évaporation est principalement fonction de l'étirement auxquelles ces structures sont soumises. C'est en effet celui-ci qui conditionne le taux d'évacuation de cette vapeur interstitielle, ce qui permet de mettre les gouttes en contact avec un environnement plus sec, déclenchant ainsi leurs évaporations.Une deuxième partie s'intéresse à une application de la compréhension de cette dynamique d'évaporation de spray dense à un contexte d'entomologie: la chimiotaxie de la mite. Il s'agit du processus par lequel l'animal va se repérer dans un champ de concentration, pour localiser la source d'une substance attractive par exemple (ici de la phéromone sexuelle). Cette partie a tendu vers l'obtention d'une visualisation conjointe de la trajectoire de l'animal et du champ de concentration sous-jacent par la visualisation des gouttes. / This thesis present some experiments on droplets evaporation and moth chemotaxis.In a first part, dense spray evaporation is explained through a new approach, drawing an analogy with scalar mixing. In this high density droplets limit, where vapor saturation is reached within the structures, evaporation is mainly controlled by the intensity at which these structures are stretch. It is indeed the stretching that controls the rate at witch interstitial vapor is evacuated: droplets are thus in contact with a dryer environment, so they can start to evaporate.A second part take an interest in applying dense spray evaporation dynamic understanding to an entomologist situation: moth chemotaxis. This is the process by which animals find their way in a concentration field, such at finding a chemoattractant source for instance (sexual pheromone in our case). This part tended towards visualizing animal trajectory and concentration field underlying through droplets visualization simultaneously.
372

Processo de microfiltração de efluente da indústria têxtil utilizando membrana de fibra oca - modelagem e simulação. / Textile industry effluent microfiltration process using hollow fiber membrane – modelling and simulation.

LIRA, Daniel Silveira. 17 October 2018 (has links)
Submitted by Maria Medeiros (maria.dilva1@ufcg.edu.br) on 2018-10-17T14:32:29Z No. of bitstreams: 1 DANIEL SILVEIRA LIRA - DISSERTAÇÃO (PPGEQ) 2018.pdf: 2803681 bytes, checksum: 57ec3900710d6a6bdb50e2ac6a6a1f79 (MD5) / Made available in DSpace on 2018-10-17T14:32:29Z (GMT). No. of bitstreams: 1 DANIEL SILVEIRA LIRA - DISSERTAÇÃO (PPGEQ) 2018.pdf: 2803681 bytes, checksum: 57ec3900710d6a6bdb50e2ac6a6a1f79 (MD5) Previous issue date: 2018-04-25 / Capes / As indústrias têxteis são, em geral, grandes consumidoras de água, devido principalmente a seus processos de tingimento de tecidos, consequentemente, as indústrias têxteis se apresentam como grandes geradoras de efluentes. A esses efluentes, estão associadas grande número de produtos químicos, dentre estes produtos, podem-se destacar os corantes, que conferem à água cor indesejável, além de contribuir com o aumento da DBO e DQO do efluente. O índigo é um corante comumente utilizado nas indústrias têxteis, principalmente no tingimento de tecidos de jeans e brim. Dentre os processos de tratamento utilizados para realizar a separação do índigo da água, podem ser destacados os processos de separação por membrana, que são um processo físico de separação baseado na utilização de uma barreira semipermeável para separar os componentes. Um dos problemas mais comuns do processo de membranas é a formação de uma camada de polarização de concentração. Este trabalho teve o objetivo de estudar o processo de separação da água-índigo utilizando membranas. Para isto foi realizado um estudo de malha utilizando o método do Índice de convergência de malha, além de, definir um modelo fluidodinâmico baseado em uma abordagem de mistura multicomponente para estudar os fenômenos de transferência de massa. Além disso, foi utilizado o modelo das resistências-em-série para simular a formação da camada limite de polarização da concentração. As malhas foram geradas utilizando o software ICEM CFD 15.0, já as simulações foram feitas utilizando pacote computacional Ansys CFX 15.0, também foi desenvolvido código em VBA para determinação da espessura da camada limite de concentração. Tendo como base os resultados do ICM, foi escolhida uma malha com 87759 elementos para seguir o estudo fluidodinâmico da membrana. Já o estudo fluidodinâmico concluiu que as variáveis número de Reynolds e pressão influenciaram o comportamento da camada de polarização de concentração, já a variável concentração de soluto na alimentação não influenciou de forma significativa. / The textile industries are, in general, major water consumers, mainly due to their garment dyeing processes, consequently, the textile industries are presented as great effluent generators. These effluents are associated with a great quantity of chemical products, within these products, the dyes stand out, which confer to the water an undesirable color, besides contributing to the increase of BOD and COD of the effluent. The indigo dye is a commonly used dye in the textile industry, mainly for the coloring of jeans and denim garments. From the water treatment processes used to separate indigo from water, the membrane separation processes can be highlighted, these comprise physical separation process’ based on the use of a semipermeable barrier to separate the components. One of the major problems in the membrane separation processes is the formation of a concentration polarization layer. This work had the aim to study the indigo-water separation process using membranes. For so, a grid convergence study was realized, in addition to defining a fluid dynamical model based on a multicomponent mixture approach to study the mass transfer phenomena. Also, the resistance-in-series model was used to simulate the formation of the concentration polarization layer. The grids were generated using the software ICEM CFD 15.0, while the simulations were done using the computational package Ansys CFX 15.0, also, a VBA code was developed do determine the thickness of the concentration boundary layer. Results showed that, based on the Grid Convergence Index, a grid with 87759 elements was chosen to follow through with the study. While the fluid dynamics study concluded that the Reynolds number and outlet pressure variables influenced on the behavior of the concentration polarization layer, however, the inlet solute concentration variable did not influence in a significant manner.
373

Computational Analyses of Complex Flows with Chemical Reactions

January 2012 (has links)
abstract: The heat and mass transfer phenomena in micro-scale for the mass transfer phenomena on drug in cylindrical matrix system, the simulation of oxygen/drug diffusion in a three dimensional capillary network, and a reduced chemical kinetic modeling of gas turbine combustion for Jet propellant-10 have been studied numerically. For the numerical analysis of the mass transfer phenomena on drug in cylindrical matrix system, the governing equations are derived from the cylindrical matrix systems, Krogh cylinder model, which modeling system is comprised of a capillary to a surrounding cylinder tissue along with the arterial distance to veins. ADI (Alternative Direction Implicit) scheme and Thomas algorithm are applied to solve the nonlinear partial differential equations (PDEs). This study shows that the important factors which have an effect on the drug penetration depth to the tissue are the mass diffusivity and the consumption of relevant species during the time allowed for diffusion to the brain tissue. Also, a computational fluid dynamics (CFD) model has been developed to simulate the blood flow and oxygen/drug diffusion in a three dimensional capillary network, which are satisfied in the physiological range of a typical capillary. A three dimensional geometry has been constructed to replicate the one studied by Secomb et al. (2000), and the computational framework features a non-Newtonian viscosity model for blood, the oxygen transport model including in oxygen-hemoglobin dissociation and wall flux due to tissue absorption, as well as an ability to study the diffusion of drugs and other materials in the capillary streams. Finally, a chemical kinetic mechanism of JP-10 has been compiled and validated for a wide range of combustion regimes, covering pressures of 1atm to 40atm with temperature ranges of 1,200 K - 1,700 K, which is being studied as a possible Jet propellant for the Pulse Detonation Engine (PDE) and other high-speed flight applications such as hypersonic missiles. The comprehensive skeletal mechanism consists of 58 species and 315 reactions including in CPD, Benzene formation process by the theory for polycyclic aromatic hydrocarbons (PAH) and soot formation process on the constant volume combustor, premixed flame characteristics. / Dissertation/Thesis / Ph.D. Aerospace Engineering 2012
374

[en] HEAT AND MASS TRANSFER COOLING OF A VERTICAL SURFACE BY A LIQUID FILM WITH AIR FLOW / [pt] TRANSFERÊNCIA DE CALOR E MASSA NO RESFRIAMENTO DE UMA SUPERFÍCIE VERTICAL ATRAVÉS DE UM FILME LÍQUIDO COM ESCOAMENTO DE AR

MARIA REGINA DA SILVA GALETTI 12 April 2012 (has links)
[pt] Uma das características das alternativas consideradas para o desenvolvimento de reatores nucleares com características avançadas de segurança consiste na utilização de resfriamento do vaso de contenção com um fluxo de água vindo de seu topo. Este sistema forma um escoamento de um filme líquido evaporando sobre uma superfície metálica aquecida com ar escoando em contra-corrente. O presente trabalho consiste no desenvolvimento de um modelo analítico e numérico da transferência de calor e massa que ocorre na interface entre o filme líquido e a mistura ar-vapor, neste tipo de escoamento e também em escoamentos co-correntes. Devido à ausência de dados experimentais, o modelo é validado através de comparações com resultados analíticos disponíveis na literatura para situações próximas aos casos de interesse. A influência de diversos parâmetros geométricos e operacionais é analisada e são fornecidos resultados para os números de Nusselt e de Sherwood observados na interface do escoamento das fases. / [en] The design of so called Advanced Nuclear reactors includes many features of passive safety, one of them being a water flow system for cooling the containment vessel. This system generates a counter-current flow pattern with a downward liquid film evaporating over the heated vessel surface and na upward stream of air. This work presents an analytical and numerical model for analysis of heat and mass transfer occuring in the interface formed by water film surfece anda ir-vapor mixture in counter-curent and parallel flows. Due to the lack of experimental data, the model is validated throught comparisons with analytical results found in the flow analysed and the results for Nusselt and Sherwood numbers are presented.
375

[en] HEAT AND MASS TRANSFER BETWEEN LIQUID FILM AND AIR STREAM / [pt] TRANSFERÊNCIA DE CALOR E MASSA ENTRE FILME LÍQUIDO E CORRENTE DE AR

PAULO MURILLO DE SOUZA ARAÚJO 18 October 2012 (has links)
[pt] O objetivo deste trabalho é analisar as transferências de momentum, calor e massa num canal bidimensional inclinado, onde escoam um filme líquido descendente e uma corrente turbulenta de ar de sentido oposto, ou de mesmo sentido oposto, ou de mesmo sentido. O filme líquido, suposto laminar, consiste numa solução fraca, ou degenerada, de trietileno glicol em água. A placa inferior do canal é mantida aquecida numa temperatura uniforme, de modo a facilitar a remoção de água do líquido para a fase gasosa. A corrente de ar deve ser turbulenta, para garantir taxas convenientes da massa de água transferida. Considera-se, não obstante, a possibilidade do ar também escoar liminarmente. A placa superior do canal, ou cobertura é adiabática e ambas as placas são impermeáveis à transferência de água. A parte hidrodinamica do problema é resolvida separadamente. Atribui-se maior importância à determinação dos perfis de temperatura e concentração de águas nas duas fases. Pretende-se que a aparelhagem acima descrita opere como um regenerador da substância líquida higroscópica, tendo este sido previamente usado num secador de ar, em aplicação de fim industrial ou agrícola. A tarefa proposta pelo problema é a simulação das condições operativas do trocador de massa. Como resultado desta simulação, tenciona-se predizer os valores dos coeficientes de transferências de calor e massa, variando amplamente as taxas de escoamento, tanto da fase gasosa, quanto da líquida. Na verdade, diversos pesquisadores têm revelado, nos últimos anos, um grande interesse no estudo de regeneradores do tipo aqui analisado. Toda vez que se tem disponibilidade de energia a temperaturas moderadas e baixo custo, como energia solar ou calor de rejeito industrial, parece indicado regenerar desta forma o desumidificante líquido nas instalações de condicionamento de ar por resfriamento evaporativo. Estabelecidas as equações diferenciais parciais do problema e as condições de contorno pertinentes, elas são resolvidas através de algoritmos obtidos por diferenças finitas, dentro do enfoque de volumes de controle. O procedimento numérico é interativo, usando-se o computador digital na obtenção da solução. Verifica-se que os resultados se mantêm dentro da analogia entre transferência de calor e massa, conforme era esperado. A partir dos resultados, podem-se estabelecer algumas correlações para os principais parâmetros do problema. Propõe-se, por fim, uma metodologia para o projeto do equipamento. Para isto, não é necessário fazer uso direto do método numérico, pois existem algumas poucas equações analíticas, simples, que podem ser facilmente manipuladas num microcomputador ou, até mesmo, numa calculadora eletrônica. Estas equações são deduzidas a partir da aplicação da teoria de penetração ao problema. As correlações dos resultados numéricos são, entretanto, essenciais ao bom emprego da teoria penetração. / [en] The combined momentum, heat and mass transfer is analysed in a two domensional inclined channnel for a countercurrent, or co-current turbulent air strem flowing past a liquid falling film. The film flow, supposed to be laminar, consists of a weak, or co-current tubulent air stream flowing past a liquid falling film. The film flow, supposed to be laminar, consists of a weak, or degenerate, solution of triethylene glycol and water. The film flow, supposed to be laminar, consists of a weak, or degenerate, solution of triethylene glycol and water. The lower plate of the channel is maintained at a constant, relatively high from the liquid to the gas phase. The stream of air is usually turbulent, thus assuring convenient rates of tranferred mass of water. Nevertheless, the possibility of laminar flow in the gas is not avoided. The second plate of the channel is considered as adiabatic and both plates are impervious to water. The hydrodynamic part of the problem is solved separately, and the determination of temperature and concentration of water profiles in the two phases is of major importance. The apparatus above described is intended to be a regenerator of the hygroscopic liquid, previously used in an air dryer, for industrial or agricultural purposes. The tash suggested by the problem is then to simulate the conditions, under which this mass exchanger will operate. Following the simulation, heat and mass transfer coefficientes can be predicted for a large range of flow rates of both gas an liquid phases. In fact, in recent years several investigators have manifested an increasing interest in developing studies of such equipment. In situations where a source of energy at low temperature is freely available, and this is the case of solar energy or industrial rejects, the employment of liquid dehumidifier regenerators is particularly attractive for evaporative cooling air conditioning systems. The partial differential equations of the problem, accompanied by suitable boundary conditions, are solved by a finite difference scheme, based on the volume of control approach. There are iterative procedures involved and solutions is reached in a mainframe computer. The results seem to be in accordance with the expected analoggy between heat and mass transfer. Some correlations are presented for the principal parameters of the problem. Lastly, a methodology is proposed for the design of the equipment. In spite of the complexity of the problem, it is possible to provide the user with a few simple analytic equations, which can be solved in any micro-computer or even in a pocket calculation. Theses equations arise from the employment of the penetration theorym briefly discussed and compared with numerical results. Indubitably, the use of this theory must be in compliance with the previously obtained numerical correlations.
376

Efeito do uso de glicerol residual e carreadores de oxigênio sobre a produção de lipases de Staphylococcus warneri EX17

Rech, Fernanda Roberta January 2011 (has links)
A transferência de oxigênio é um fator limitante para grande parte dos cultivos em biorreatores que operam com organismos estritamente aeróbios devido à baixa solubilidade do oxigênio em meios de cultivo. A introdução de meios não convencionais, como polidimetilsiloxanos (PDMS), em biorreatores pode ser vista como uma melhoria para o ajuste desses processos. Este trabalho propõe uma investigação do emprego de polidimetilsiloxano fluido e emulsificado, usados como carreadores de oxigênio, em meios de cultivo na produção de lipases de Staphylococcus warneri EX17, cepa capaz de utilizar glicerol residual como substrato, visando melhorar a disponibilidade de oxigênio e aperfeiçoar a produtividade. Inicialmente, foram realizados estudos para selecionar o uso de glicerol residual, oriundo da síntese química de biodiesel como fonte de carbono. A atividade lipolítica foi semelhante com glicerol comercial e residual. Após a seleção do glicerol foram realizados experimentos em incubadora rotatória horizontal nas condições previamente otimizadas em trabalhos anteriores do grupo, adicionando PDMS. Dois tipos de PDMS foram testados, fluido e emulsificado. A produção de lipase foi significativamente maior no meio contendo PDMS. Assim, ferramentas de planejamento experimental, delineamento composto central (DCC) foram utilizadas para verificar a influencia do PDMS no coeficiente de transferência de oxigênio kLa em meio de cultivo produtor de lipase livre de células. O kLa aumentou significativamente no meio de cultivo contendo PDMS. Dois novos planejamentos foram desenhados, para otimizar a produção de lipase em meio de cultivo com células. Um para PDMS fluido e outro para PDMS emulsificado. No meio contendo PDMS fluido, a atividade lipolítica foi cinco vezes maior, enquanto que no meio contendo silicone emulsificado a atividade lipolítica foi três vezes maior. Este estudo demonstrou que a lipase de S. warneri EX17 pode ser produzida utilizando glicerol residual como fonte de carbono, e polidimetilsiloxanos como carreadores de oxigênio aumentando a transferência de oxigênio no meio e elevando a produção da enzima, que apresenta diversas possibilidades de aplicação, principalmente na indústria de alimentos. / The oxygen transfer is a limiting factor in many bioreactors cultivations, in which strictly aerobic organisms are grown due to the low solubility of oxygen in culture media. The introduction of unconventional media, such as polydimethylsiloxanes (PDMS) in bioreactors can be seen as a potential improvement for these processes. This work proposes an investigation of the use of polydimethylsiloxane in its two forms, fluid and emulsified, used as carriers of oxygen in culture media for the lipase production of Staphylococcus warneri EX17, strain capable of using glycerol as substrate, to improve the oxygen availability and improve enzyme productivity. Initially, studies were performed to select the use of residual glycerol, derived from the chemical synthesis of biodiesel as a source of carbon. The lipase activity was similar in both commercial and residual glycerol. Experiments were performed on orbital shaker, using conditions previously optimized in another work of the group, but with the addition of PDMS. Two types of PDMS were tested, the fluid and the emulsified. The lipase production was significantly higher in medium containing PDMS. Experimental design and central composite design (CCD) were used to evaluate the influence of PDMS on the oxygen transfer coefficient kLa in culture medium free of lipase-producing cells. The kLa increased significantly in the medium containing PDMS. Two new experimental plannings were designed to optimize the production of lipase in culture medium with cells. In the medium containing the PDMS fluid lipase activity was five times higher than medium without the oxygen carrier, while in the medium containing the emulsified silicone lipase activity was three times higher. This study showed that the lipase from S. warneri EX17 can be produced using residual glycerol as carbon source and that polydimethylsiloxanes works as interesting carrier of oxygen by increasing oxygen transfer rates, improving enzyme production, which has several possible applications, especially in the food industry.
377

The transport of mass and energy in toroidal fusion machines

Deane, G. B. January 1989 (has links)
To understand the physical mechanism underlying the cross-field transport of mass and energy in magnetoplasmas is a long-standing problem in fusion research. Woods (1987) has recently developed a second-order transport theory which has been used to explain a number of transport-related phenomena observed in tokamaks. Here, we apply second-order transport theory to the reverse field pinch (RFP) and a phenomenon observed in tokamaks known as 'snakes'. Expressions for the mass and energy confinement times in the RFP, τ<sub>p</sub> and τ<sub>e</sub>, are deduced and agreement with experimental results from HBTX is found. For typical operating conditions the times τ<sub>p</sub> ~ 0.1ms and τ<sub>e</sub> ~ 0.2ms are observed in HBTX. Second-order transport theory predicts τ<sub>p</sub> ~ 0.4ms and τ<sub>e</sub> ~ 0.4ms for this machine. Scaling laws for β<sub>p</sub> versus η<sub>e</sub>,β<sub>p</sub> versus I<sub>φ</sub> and τ<sub>e</sub> versus I<sub>φ</sub> are compared with measurements from HBTX and agree well with observation. Snakes are large density perturbations observed in JET after fuel pellet injection. Typical snakes in JET are remarkably stable and are found to have density decay times longer than predictions based on neoclassical theory (Stringer 1987). After their formation, snakes have even been observed to grow (Weller <i>et al.</i> 1987), which suggests the presence of an inward diffusion mechanism. There is also some evidence for a temperature depression in the snakes region. An explanation of the stability and energy balance in snakes based on second-order transport theory is proposed.
378

A MODEL FOR THE PREDICTION OF THERMAL RESPONSE OF BONE IN SURGICAL DRILLING

Maani, Nazanin 01 August 2013 (has links)
This Thesis develops a mathematical model for predicting the thermal response in the surgical drilling of bone. The model accounts for the bone, chip and drill bit interactions by providing a detailed account of events within a cylindrical control volume enveloping the drill, the cut bone chip within the drill bit flute and the solid bone. Lumped parameter approach divides the control volume into a number of cells and cells within the sub-volumes representing the drill solid, the bone chip and the bone solid are allowed to interact. The contact mechanics of rough surfaces is used to model chip-flute and chip-bone frictional interaction. In this way not only the quantification of friction due to sliding contact of chip-flute and chip-bone rough surface contact are treated, but also the contact thermal resistances between the rubbing surfaces are included in the model. A mixed combination of constant and adaptive mesh is employed to permit the simulation of the heat transfer as the drill bit penetrates deeper into the bone during a drilling process. Using the model the effect of various parameters on the temperature rise in bone, drill and the chip are investigated. It is found that maximum temperature within the bone occurs at the location adjacent to the corner of the drill-tip and drill body. The results of the model are found to agree favorably with the experimental measurements reported in the existing literature on surgical drilling.
379

Modélisation de réacteurs Gaz-Liquide de type colonne à bulles en conditions industrielles / Modelling of gas-liquid bubble column reactors under industrial conditions

Colombet, Damien 11 July 2012 (has links)
L’oxydation du cyclohexane est l’un des procédés les plus importants dans la chaîne de production du Nylon où l’oxygène et le cyclohexane entrent en contact pour former le cyclohexanol, la cyclohexanone puis l’acide adipique. Le rendement est influencé à la fois par le transfert de l’oxygène et par le mélange des réactifs en phase liquide. Des réacteurs de type colonne à bulles sont généralement utilisés pour fournir une aire interfaciale importante et garantir une agitation efficace en phase liquide. Cependant, la complexité des mécanismes impliqués (hydrodynamique, transfert, réaction, fort taux de vide) rend difficile la prédiction des performances des réacteurs. Ce travail est consacré à l’amélioration des lois de fermetures (quantité de mouvement et transferts) pour la modélisation Euler/Euler des réacteurs industriels utilisés pour le procédé d’oxydation du cyclohexane. Dans un premier temps, des expériences de laboratoire avec le système eau/air ont été réalisées jusqu’à de forts taux de vide (> 30%) pour mesurer les effets collectifs sur la force de traînée et le transfert de masse dans un essaim de bulles homogène. Les résultats ont confirmé que le coefficient de traînée des bulles augmente de manière significative avec le taux de vide alors que de manière surprenante l’effet est très faible sur le transfert. Dans un second temps, des expériences ont été réalisées avec le système cyclohexane/diazote dans des conditions industrielles (P = 1 - 20 bar, T = 30 - 150°C). L’analyse des résultats de transfert en condition industrielle a nécessité la simulation numérique directe du transfert à l’intérieur d’une bulle sphérique / Cyclohexane oxidation is one of the most important processes in the production line of Nylon, where oxygen and cyclohexane get in contact to produce cyclohexanol, cyclohexanone and then adipic acid. The production yield is influenced by both the oxygen transfer and the reactants mixing in liquid phase. Bubble column type reactors are usually used to provide a large interfacial area and efficient liquid phase agitation. However, the complexity of the mechanisms involved (hydrodynamic, transfer, reaction, high void fraction) makes it difficult to predict the performance of such reactors. This work is devoted to improve the associated closure laws of momentum and transfer equations used in Euler/Euler modelling of industrial reactors for cyclohexane oxidation. Bench-scale experiment for air-water system has been firstly carried out to measure the collective effects on the drag force and the mass transfer of a bubble in a homogenous bubble swarm with a high void fraction up to 30%. The results confirmed that bubble’s drag coefficient increases significantly with the void fraction. Meanwhile surprisingly, weak effect has been observed on the transfer. Nextly, pilot experiments with nitrogen-cyclohexane system have been performed under industrial conditions (P = 1 - 20 bar, T = 30 - 150°C). Analysis of the results of transfer under industrial conditions required finally direct numerical simulation of transfers inside a spherical bubble.
380

Efeito do uso de glicerol residual e carreadores de oxigênio sobre a produção de lipases de Staphylococcus warneri EX17

Rech, Fernanda Roberta January 2011 (has links)
A transferência de oxigênio é um fator limitante para grande parte dos cultivos em biorreatores que operam com organismos estritamente aeróbios devido à baixa solubilidade do oxigênio em meios de cultivo. A introdução de meios não convencionais, como polidimetilsiloxanos (PDMS), em biorreatores pode ser vista como uma melhoria para o ajuste desses processos. Este trabalho propõe uma investigação do emprego de polidimetilsiloxano fluido e emulsificado, usados como carreadores de oxigênio, em meios de cultivo na produção de lipases de Staphylococcus warneri EX17, cepa capaz de utilizar glicerol residual como substrato, visando melhorar a disponibilidade de oxigênio e aperfeiçoar a produtividade. Inicialmente, foram realizados estudos para selecionar o uso de glicerol residual, oriundo da síntese química de biodiesel como fonte de carbono. A atividade lipolítica foi semelhante com glicerol comercial e residual. Após a seleção do glicerol foram realizados experimentos em incubadora rotatória horizontal nas condições previamente otimizadas em trabalhos anteriores do grupo, adicionando PDMS. Dois tipos de PDMS foram testados, fluido e emulsificado. A produção de lipase foi significativamente maior no meio contendo PDMS. Assim, ferramentas de planejamento experimental, delineamento composto central (DCC) foram utilizadas para verificar a influencia do PDMS no coeficiente de transferência de oxigênio kLa em meio de cultivo produtor de lipase livre de células. O kLa aumentou significativamente no meio de cultivo contendo PDMS. Dois novos planejamentos foram desenhados, para otimizar a produção de lipase em meio de cultivo com células. Um para PDMS fluido e outro para PDMS emulsificado. No meio contendo PDMS fluido, a atividade lipolítica foi cinco vezes maior, enquanto que no meio contendo silicone emulsificado a atividade lipolítica foi três vezes maior. Este estudo demonstrou que a lipase de S. warneri EX17 pode ser produzida utilizando glicerol residual como fonte de carbono, e polidimetilsiloxanos como carreadores de oxigênio aumentando a transferência de oxigênio no meio e elevando a produção da enzima, que apresenta diversas possibilidades de aplicação, principalmente na indústria de alimentos. / The oxygen transfer is a limiting factor in many bioreactors cultivations, in which strictly aerobic organisms are grown due to the low solubility of oxygen in culture media. The introduction of unconventional media, such as polydimethylsiloxanes (PDMS) in bioreactors can be seen as a potential improvement for these processes. This work proposes an investigation of the use of polydimethylsiloxane in its two forms, fluid and emulsified, used as carriers of oxygen in culture media for the lipase production of Staphylococcus warneri EX17, strain capable of using glycerol as substrate, to improve the oxygen availability and improve enzyme productivity. Initially, studies were performed to select the use of residual glycerol, derived from the chemical synthesis of biodiesel as a source of carbon. The lipase activity was similar in both commercial and residual glycerol. Experiments were performed on orbital shaker, using conditions previously optimized in another work of the group, but with the addition of PDMS. Two types of PDMS were tested, the fluid and the emulsified. The lipase production was significantly higher in medium containing PDMS. Experimental design and central composite design (CCD) were used to evaluate the influence of PDMS on the oxygen transfer coefficient kLa in culture medium free of lipase-producing cells. The kLa increased significantly in the medium containing PDMS. Two new experimental plannings were designed to optimize the production of lipase in culture medium with cells. In the medium containing the PDMS fluid lipase activity was five times higher than medium without the oxygen carrier, while in the medium containing the emulsified silicone lipase activity was three times higher. This study showed that the lipase from S. warneri EX17 can be produced using residual glycerol as carbon source and that polydimethylsiloxanes works as interesting carrier of oxygen by increasing oxygen transfer rates, improving enzyme production, which has several possible applications, especially in the food industry.

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