ABSTRACT   Migration is common among populations of brown trout (Salmo trutta) and Atlantic salmon (Salmo salar). However, not all individuals in the same population migrate, a phenomenon referred to as partial migration. The aim of this thesis was to investigate if an individual’s behaviour and metabolic rate influences its decision to migrate and how such knowledge may be used when trying to produce hatchery-raised smolts with as high a proportion of migrating individuals as possible. In paper I the influence of reduced food ration on the proportion and swimming speed of migrating brown trout and Atlantic salmon smolts was investigated. Furthermore, the standard metabolic rate (SMR) of migrating and non-migrating individuals was compared. In paper II, a laboratory experiment, SMR was correlated to the behaviour of individual brown trout and Atlantic salmon. Dominant fish of both species had a higher SMR than subordinates (paper II). In addition, migrant brown trout had a higher SMR than non-migrant trout when given a normal food ration, whereas no difference in SMR between migrating and non-migrating salmon could be seen (paper I). When administered low food rations, smolts of both species migrated faster than smolts given a normal food ration, and the proportion of migrating smolts was higher for salmon given less food when the size difference for smolts from the two feeding regimes was large (paper I). Other factors that influenced migration speed were the degree of smolt development and water temperature (paper I). SMR was not correlated with aggressiveness, or with different measurements of boldness. Moreover, aggression and boldness were not correlated with each other (paper II). Trout showed a higher level of aggressiveness and acclimated more rapidly to laboratory conditions than salmon (paper II). In summary, there was no support for the existence of coping styles in migratory Atlantic salmon and brown trout. Instead, metabolic rates were related to both migratory behaviour and social status. Furthermore, an individual’s decision to migrate was influenced by ration size.

Social insects are used as models for understanding the evolution of sociality because they show seemingly complex behavioral and physiological traits that enforce group cohesion, collective organization, and group level reproduction. Social organization in insect societies requires workers to share information. Information sharing allows workers to efficiently perform and switch among tasks to meet colony needs. For many species that nest in preformed cavities, colony growth results in crowding inside the nest which can affect colony productivity and fitness. How does colony size and social density affect individual and collective behavior? Using a combination of laboratory and field experiments, I have begun to answer this question. In Temnothorax rugatulus ants, high social density resulted in greater colony energy use. In addition, larger colonies used proportionally less energy compared to smaller colonies, but showed reduced brood production. These results indicate that the way colonies use energy changes with social density and group size. In analyzing the effects of colony size and density on worker behavior, I found that high density increased worker connectivity and information sharing. Workers in larger colonies showed less connectivity compared to workers in smaller colonies. Interestingly, workers with more interactions spent less time in brood care. This study shows that workers' access to information and the overall pattern of information flow are affected by social density and colony size, and changes in worker connectivity can influence task behavior. The next study shows that field colonies maintained a relatively constant level of intranidal density irrespective of colony size; this suggests that Temnothorax ants actively regulate social density. When colonies were established in high density nests, they showed greater foraging and scouting activities, and this led to a higher probability for becoming polydomous, i.e., occupying multiple nests. When polydomy occurred, colonies divided evenly between two nests, but distributed fewer, heavier workers and brood to the supplemental nests. Taken together, the first four studies indicate that social density is an important colony phenotype that affects individual and collective behavior and energetics in ants, and the collective management of social density may be a group adaptation in ants and other social insects. Lastly, because crowding affects polydomy behavior, the final two experiments tested whether colony emigration and nest construction and dispersion, two strategies for reducing intranidal crowding, are influenced by food distribution. Temnothorax colonies preferred to emigrate to nests positioned closer to food, and weaver ants (Oecophylla smaragdina) positioned newly constructed nests in food-rich areas. Furthermore, weaver ants used the newly constructed nests to more rapidly retrieve and safeguard valuable food items. Thus, strategic emigrations and adaptive nest dispersion can remedy intranidal crowding and at the same time allow growing colonies to acquire adequate food to meet colony needs.

Temperature variation affects most biological parameters from the molecular level to community structure and dynamics. Current studies on thermal biology assess how populations vary in response to environmental temperature, which can help determine how populations differentially respond to climate change. To date, temperature fluctuation effects on endothermic poikilotherms such as the common eastern bumblebee (Bombus impatiens) are unknown even though bumblebees are the most important natural pollinators in North America. A cold-acclimation experiment with B. impatiens colonies revealed individuals acclimated to 5°C or 10°C at night did not differ in resting metabolic rate, flight metabolic rate, wingbeat frequency, or morphological measurements, compared to the control group. Moreover, an infrared camera showed that all colonies maintained maximum nest temperature consistently above 36.8°C. A latitudinal sampling of flight metabolic rate and morphological measurements of B. impatiens from four locations spanning Ontario (N 45°; W 75°) to North Carolina (N 34°; W 77°) indicated no latitudinal trend in the measured variables. This study shows that bumblebees are well equipped to face a wide range of environmental temperatures, both in the short term and long term, and can use a combination of behavioural and physiological mechanisms to regulate body and nest temperatures. These results are reassuring on the direct effects of climate change on bumblebee ecology, but further studies on the indirect effect of temperature variation on North American bumblebees are required to predict future ecosystem dynamics.

‘Animal personality’ refers to individual behavioural differences that are consistent over time and context. Physiological constraints are suggested to underlie this constraint in behavioural plasticity. As energy is required for physiological processes that generate behaviour, energy metabolism could be a proximate explanation for personality. Currently, the most coherent framework linking behaviour, metabolism and life history-traits is still poorly tested empirically, and studies are showing contradicting results. Therefore, I here aim to explore this relationship further by investigating the relationship between basal metabolic rate (BMR) and personality in Red Junglefowl (Gallus gallus). Birds used had known responses in personality assays, and their metabolic rates were measured by determining oxygen consumption in standardized conditions throughout the night using an open respirometry system. BMR was negatively correlated with time spent foraging, and positively correlated with time spent being vigilant. Considering foraging an ‘activity’ (due to its energy-demand), my results support the allocation model, a model that assumes that an animal has a fixed amount of energy, thus that an energetic trade-off occur between competing energy requiring processes such as BMR and activity. Hence, an animal with low BMR has more energy to spend on activity. However, I do not consider vigilance as an energy-demanding activity; hence this relationship cannot be interpreted in this framework. Taken together, my results show a relationship between personality and BMR, although their relationship still needs further investigation to understand the causality and consequences of it.

With regard to heat stress, the limiting factor inherent in clothing ensembles is the total evaporative resistance. Clothing with higher evaporative resistance impedes the ability to cool by sweat evaporation. Knowing the evaporative resistance provides a means to compare candidate ensembles. Further, a value for evaporative resistance means that a rational method can be used to assess the heat stress exposure. Evaporative resistance of five clothing ensembles (cotton work clothes, cotton coveralls, and three coveralls of particle barrier, liquid barrier and vapor barrier properties) was determined empirically from wear tests during two study phases. For Phase 1, the metabolic rate was held constant at 160 W/m², and three levels of humidity (20, 50, 70% rh) were explored. Fourteen heat-acclimated participants (9 men and 5 women) completed trials for all combinations of clothing ensemble and environment. In the Phase 2 study, the humidity was held constant at 50% rh, and three levels of metabolic rate (114, 176, 250 W/m²) were explored. Fifteen heat-acclimated participants (11 men and 4 women) completed trials for all combinations of clothing ensemble and environment. The data from both phases were analyzed separately using ANOVA. Significant differences were found among ensembles (p<0.0001). The vapor barrier ensemble had the highest resistance at 0.026 kPa m²/W. The liquid barrier was next at 0.018; followed by the particle barrier and cotton coveralls at 0.016. Work clothes was 0.014 kPa m²/W. Vapor and liquid barrier ensembles were found to be significantly different from other ensembles. From the Phase 2 study, evaporative resistances decreased with increased activity and ranged from 0.0024 (cotton coveralls) to 0.0094 (vapor barrier) kPa m²/W. The higher differences were associated with higher total evaporative resistance. The decreased evaporative resistances in Phase 2 can be explained by the pumping action associated with increased work. The relationship of Re,T to the difference of Pair -- Pskin was explored and found Re,T does not remain constant. Environment appeared to influence this relationship.

The use of mouthwash is a common complement to oral care. However, the physiological implication of this use, besides of effects on oral hygiene, is poorly known. The research of the gut micro flora and its implications on the host is a very active area of research today. Many important connections between the gut micro flora and obesity and diabetes have been found. These billions of bacteria are part of the immune system, they produce essential vitamins and they make inaccessible polysaccharides more digestible to the host, just to mention a few of their symbiotic roles for the host. A less explored area is the micro flora in the oral cavity. On the back of the tongue, anaerobic bacteria can reduce dietary nitrate to nitrite which then further can be reduced to nitric oxide, NO. NO is important in several important biological functions, e.g. as a signal substance, vasoregulation, mucus production and antibacterial effects. Vegetables as beetroot and spinach are dietary sources with a high nitrate content. Also drinking water and processed meats can be of relevance. Nitrite is added to processed meat for the prevention of botulism but also adds taste and color.   Experiments on humans indicate that mitochondrial efficiency increases after nitrate load, manifested as a decreased oxygen demand during physical exercise. This can also be relevant under conditions where the mitochondrial function is impaired, such as in diabetes and cardiovascular diseases. First a pilot study was made to evaluate the nitrate reducing effect from the antibacterial mouthwash. The mouthwash proved very effective. The concentrations of nitrate and nitrite in saliva was analyzed by HPLC and saliva from the antibacterial treatment showed greatly reduced concentrations of nitrite and high concentrations of nitrate. Saliva from placebo mouthwash showed high concentrations of nitrite and low concentrations of nitrate as expected. To study the importance of oral bacteria on metabolism, we performed a randomized, cross-over double-blinded study with 19 healthy males between 22-43 years. During two separate three-day periods they used an antibacterial and placebo mouthwash, respectively. On the fourth day their basal metabolic rate (BMR) was measured with an indirect calorimetric system. Moreover, samples from saliva, urine and blood were collected but these results are not included in this thesis. An earlier, unpublished study has demonstrated that nitrate administration reduces the basal metabolic rate. Accordingly, our aim was to study potential effects on the basal metabolic rate following reduction of the number of oral bacteria by aid of antibacterial mouthwash. Our hypothesis was that the reduced availability of nitrite would decrease the availability of NO in the body and manifest as an increased basal metabolic rate. The results from indirect calorimetry measurements showed no significant difference between placebo and antibacterial mouthwash, but there may be confounding factors. Further study is needed to assess the potential effects on host metabolism by these bacteria.

Southern toads (Anaxyrus terrestris) are ectothermic terrestrial amphibians. As such they are sensitive to thermal and hydric variability. The interactive effect of hydration state and body temperature is not well understood. The aim of this study is to determine if the costs of dehydration vary at different temperatures and if so, whether toads will select different temperatures to minimize these costs. Costs of dehydration were evaluated by measuring metabolic rate at 100% and 85% hydration state, at 30 and 20 oC. Temperature selection of toads at 100% and 85% hydration state was measured using a thermal gradient. Body temperature, but not hydration state, was determined to have a significant effect on metabolic rate. No significant difference in temperature selection was found between toads at 100% and 85% hydration state. Thus, thermoregulatory behavior as influenced by hydration state could not be predicted in Anaxyrus terrestris.

Selenium (Se) is known to cause chronic toxicity in aquatic species. In particular, dietary exposure of fish to selenomethionine (SeMet), the primary form of Se in the diet, is of concern. Previous studies reported that chronic exposure to elevated dietary SeMet altered swimming performance, aerobic metabolism, and energy and endocrine homeostasis in adult fish. However, little is known about the direct effects of dietary SeMet exposure in juvenile fish. Therefore, the overall objective of this thesis was to investigate sublethal pathophysiological effects of subchronic dietary SeMet exposure in two juvenile fish species, fathead minnow (Pimephales promelas) and rainbow trout (Oncorhynchus mykiss). In the first experiment, 20 days post hatch (dph) juvenile fathead minnow were exposed to different measured concentrations (2.8, 5.4, 9.9, 26.5 µg Se/g dry mass [dm]) of Se in food in the form of SeMet for 60 days. In the second experiment, 14 dph juvenile rainbow trout were exposed for 37 days to different measured concentrations (1.0, 4.1, 11.2, 26.1 µg Se/g dm) of Se in food in the form of SeMet. Following exposure, samples were collected for Se analysis and fish were subjected to a swimming performance challenge to assess critical swim speed (Ucrit), tail beat frequency and tail beat amplitude, oxygen consumption (MO2), cost of transport (COT), standard metabolic rate (SMR), active metabolic rate (AMR), and factorial aerobic scope (F-AS). Dietary SeMet exposure impaired swimming ability in both fathead minnow and rainbow trout. Juvenile fathead minnow showed alterations in aerobic metabolism with increased MO2, COT and AMR at the 9.9 and 26.5 µg Se/g diets, while dietary SeMet exposure did not appear to affect aerobic metabolism in juvenile rainbow trout. After swim performance experiments, swam fish were considered fatigued and metabolic and energy storage endpoints were compared to non-swam (non-fatigued) fish. Energy storage capacity was measured via whole body (fathead minnow) and liver and muscle (rainbow trout) triglyceride and glycogen concentrations. For fathead minnow, triglyceride concentrations in non-swam fish were significantly elevated in the 5.4 µg Se/g group relative to controls, and swam fish had significantly lower whole body triglycerides than non-swam fish. All non-swam SeMet exposure groups had significantly decreased whole body glycogen concentrations compared to controls while the 5.4 and 26.5 µg Se/g exposure groups had significantly greater whole body glycogen concentrations in swam versus non-swam fish. In juvenile rainbow trout, liver triglyceride concentrations were significantly lower in all SeMet exposed groups compared to controls in non-swam fish. Swimming decreased liver and muscle triglycerides in the control and 11.2 µg Se/g treatment groups. Liver glycogen concentrations were greater in swam trout in the 4.1 µg Se/g dm exposure group. Muscle glycogen concentrations in non-swam fish, were significantly decreased in the 4.1 and 11.2 µg Se/g exposed groups compared to controls, while muscle glycogen in swam fish was unaffected by dietary SeMet exposure. For the swim status factor, muscle glycogen concentrations were significantly greater in swam versus non-swam trout in all treatment groups. Therefore, dietary SeMet exposure caused impaired swimming performance and metabolic alterations in both juvenile fathead minnow and juvenile rainbow trout. Species differences were apparent, especially in the patterns of altered energy status between swam and non-swam fish exposed to Se. Overall, the pathophysiological implications of these sublethal effects are unclear, but suggest that dietary SeMet exposure may negatively influence juvenile fish survivability in natural habitats.

Membrane diversity associated with variation in body mass and body temperature of hymenopterans was investigated. Body mass of organisms affects most aspects of their biology, including physiological traits. One trait influenced by mass is metabolic rate, but the basis for its variation among organisms remains unclear. Recent work revealed that membrane phospholipid composition varies systematically with body mass: smaller vertebrates with higher mass-specific metabolic rates have more fluid membranes containing more long chains of polyunsaturated fatty acids (PUFA) and less monounsaturated fatty acids (MUFA). This “membrane pacemaker” theory of metabolism has recently been challenged by a lack of link between metabolic rate and membrane composition. To test this relationship, we have quantified the membrane lipid composition of 22 species of Panamanian orchid bees with a 22-fold range in mass. Results incorporating phylogenetic analysis show significant relationships for 18:1 (MUFA) and 18:3 (PUFA) with body mass that are consistent with the “pacemaker” theory, and unexpected relationships with saturated fatty acids (SFA). Moreover, changes in membrane fatty acid composition with temperature are a strategy in ectothermic poïkilotherms that is part of the “homeoviscous adaptation” model. Here, we report systematic variations in fatty acid composition linked with thoracic temperature excess in North American Hymenopterans, a novel discovery in these heterotherms. These findings are discussed in the context of diet, metabolism and lifespan.

There are many situations where workers are required to wear personal protective clothing (PPC), to protect against a primary hazard, such as heat or chemicals. But the PPC can also create ergonomic problems and there are important side effects which typically increase with rising protection requirements. The most extensively studied side effect is that of increased heat strain due to reduced heat and vapour transfer from the skin. Less studied is the extra weight, bulk and stiffness of PPC garments which is likely to increase the energy requirements of the worker, reduce the range of movement and lead to impaired performance. Current heat and cold stress standards assume workers are wearing light, vapour permeable clothing. By failing to consider the metabolic effects of actual PPC garments, the standards will underestimate heat production and therefore current standards cannot be accurately applied to workers wearing PPC. Information on the effect of the clothing on the wearer and the interactions between PPC, wearer and environment is limited. Data was collected to quantify the effect of PPC on metabolic load based on the properties of the PPC for the EU THERMPROTECT project (GERD-CT-2002-00846). The main objective of the project was to provide data to allow heat and cold stress assessment standards to be updated so that they need no longer exclude specialised protective clothing. The aim of this thesis was to investigate the effect of PPC and its properties on energy consumption during work. For this purpose, the effects of a range of PPC garments (Chapter 3), weight (Chapter 4), number of layers and material friction (Chapter 5) and wet layers (Chapter 6) on energy consumption whilst walking, stepping and completing an obstacle course were studied. The impact of PPC on range of movement in the lower limbs was also investigated (Chapter 7). The main findings were; a) Increased metabolic cost of 2.4 - 20.9% when walking, stepping and completing an obstacle course in PPC compared to a control condition. b) An average metabolic rate increase of 2.7% per kg increase in clothing weight, with greater increases with clothing that is heavier on the limbs and in work requiring greater ranges of movement. c) 4.5 to 7.9% increase in metabolic cost of walking and completing an obstacle course wearing 4 layers compared to a single layer control condition of the same weight. d) Changes in range of movement in PPC due to individual behavioural adaptations. e) Garment torso bulk is the strongest correlate of an increased metabolic rate when working in PPC (r=0.828, p<0.001). f) Garment leg bulk (r=0.615), lower sleeve weight (r=0.655) and weight of the garment around the crotch (r=0.638) are also all positively correlated with an increased metabolic rate. Total clothing weight and clothing insulation had r values of 0.5 and 0.35 respectively. This thesis has confirmed the major effect of clothing on metabolic rate, and the importance of including this effect in standards and models.