For the past decade, the epoxy based photoresist SU-8 has been used commercially and in the lab for fabricating micro- and nano-structures. Investigators have studied how processing parameters such as pre- and post-exposure bake temperatures affect the resolution and quality of SU-8 structures patterned using ultraviolet or x-ray lithography. Despite the advances in understanding the phenomena, not all of them have been explored, especially those that are specific to multi-photon direct laser writing (mpDLW). Unlike conventional exposure techniques, mpDLW is an inherently three-dimensional (3D) process that is activated by nonlinear absorption of light. This dissertation reports how several key processing parameters affect mpDLW using SU-8 including pre-exposure bake duration, focal depth, incident laser power, focal-point scan speed, and excitation wavelength. An examination of solvent content of films at various stages in the mpDLW by 1H-NMR shows that even moderate solvent content (over 1 wt-%) affects film viscosity and photoacid diffusion lengths, and can greatly affect the overall fidelity of small features. A study of micro-fabricated feature size versus writing depth in the material shows that even slight refractive index mismatch between SU-8 and the medium between it and the focusing objective introduces spherical aberration that distorts the focus, causing feature size to decrease or even increase in size with writing depth, depending on the average exposure power used. Proper adjustment of the average exposure power was demonstrated as a means to fabricate more uniform features with writing depth. Third, when varying the power and scan speed, it was observed that the feature-size scales with these two parameters in a manner that is consistent with a three-photon absorption mechanism at an excitation wavelength of 800 nm. When an iii excitation wavelength of 725 nm is used, the feature-size scaling becomes consistent with that of two photon absorption. This shows that the photoinitiators in the SU-8 can be activated by either two- or three-photon absorption over this wavelength range. Using an irradiance of ~2 TW cm-2 and elongated femtosecond pulses resulted in an observed fourth order power dependence. This observation is in agreement with the literature and suggests that the effective absorptive nonlinearity is also sensitive to pulse duration. These findings will be useful for creating accurate models of the process of mpDLW in SU-8. These models could be used to optimize the processing parameters and develop new processing methods and materials for high-resolution fabrication of robust 3D microstructures. Some of the findings were used to develop a method for fabricating functional microlenses on the tip of optical fibers. This approach opens a new route to functional integrated photonic devices.

Today's mobile computing platforms provide new, convenient possibilities for location-based commercial activity. In particular, the global positioning system (GPS) provides location data for local advertising and retail activity. In this thesis, we take the next step and introduce the concept of micro check-in. Micro check-in is a simple framework that allows users to match their current location to specific objects or places at a resolution much finer than that allowed by GPS based check-in. We show that micro check-in enables customers to reveal their location at a fine level of granularity. By describing the concepts of points of service, service providers, users, and the enabling technology, we present a generalized, abstract, framework for designing, developing, and analyzing micro check-in based systems. We discuss several new applications that open up as a result of the combination of real-life and digital communication channel as a result of the micro check-in process. For example, micro check-in could prove quite useful to customers at a restaurant table, a gym equipment station, an exhibit at a museum and many more. This thesis studies micro check-in applied to the process of ordering food from a table at a restaurant and present the beneficial tradeoffs enabled by micro check-in. We find that the micro check-in framework has promising applications in several areas, but specifically utilize the restaurant application, OnTable, to prove the potential use of the micro check-in framework.

Exoskeletons are rapidly emerging from the realm of science-fiction myth to practical reality in everyday life. Various designs have provided viable means for individuals to regain capabilities that were lost or perform tasks not previously possible by their ability alone. In this research, I propose two novel exoskeletons for walking assistance and heavy load carriage. The first exoskeleton can be used to provide assistance for walking in various applications such as industrial productivity, rehabilitation, and military or space training. We introduce a design for a lower body wearable device that supports up to 80% of the user's body weight (667 N peak force) with a single actuator on each leg. Its underactuated design directs force through the user's center of mass with a single sprocket-chain driven prismatic actuator on each leg, allowing for natural gait and mobility. The device is optimized for simplicity, ease of assembly, low cost, and weight. The second design aims to counteract the one of the leading causes of injury in the workplace, repetitive and heavy lifting. The Heavy Lift and Carry Exoskeleton (HeavyLC Exo) is capable of safely lifting and carrying loads up to 36 kg (80 lbs) while minimizing the number of actuators to reduce weight and complexity. The HeavyLC Exo allows the user to direct the object, pause and hold the object steady mid-lift, and follow the natural kinematics of lifting. It is secured to the user with shoulder, chest, and dual thigh straps, along with an adjustable waist belt and overshoe attachment. Powered by two 14.8 V batteries and an off-board air compressor, the HeavyLC Exo has a total of 20 DOF, with 6 actuated DOF and 14 free DOF. The arms use only two actuators each, providing powered lifting and arm retraction/extension, and allowing a wide range of body postures; the legs are powered by single pneumatic actuators on each leg connected to the foot accompanied by a passive spring element to prevent excessive pelvic tilt and leg abduction during swing. The control system requires directional forces from the user at the tool handle of 19 N (4.3 lbf) on average. Current design limitations necessitate the user to provide up to 280 N (62.9 lbf) at the hip during worst load conditions, and future design optimization is proposed. A fully functional prototype of HeavyLC Exo is built, fully tested, and analyzed for improvement. / Master of Science / Exoskeletons, which were once only seen in science fiction, are now becoming a reality in everyday life. Various designs have made it possible for people to do things they couldn't do before or regain abilities they lost. In this research, two new exoskeletons are proposed - one for walking assistance and the other for carrying heavy loads. The first exoskeleton is designed to help people walk. It supports up to 80 % of the user's body weight with a single actuator on each leg, which directs force through the center of mass, allowing for natural gait and mobility. It's simple, easy to assemble, low-cost, and lightweight, making it useful in various applications such as medical rehabilitation, military or space training, and industrial productivity. The second exoskeleton is designed to help people lift and carry heavy objects, which is a common cause of workplace injuries. The Heavy Lift and Carry Exoskeleton (HeavyLC Exo) can safely lift and carry objects up to 36 kg (80 lbs) while minimizing the number of actuators to reduce weight and complexity. It's worn by the user using shoulder, chest, and dual thigh straps, along with an adjustable waist belt and overshoe attachment. The exoskeleton is powered by two batteries and an off-board air compressor, and has 20 degrees of freedom, with 6 powered and 14 non-powered, giving it significant flexibility to conform to lifting and walking motions allowing it to function with normal user range of motion. The arms use two actuators each to provide powered lifting and arm retraction/extension, while the legs are powered by single pneumatic actuators on each leg connected to the foot accompanied by a elastic spring element. The control system requires the user to lift and maneuver about 1.9 kg (4.2 lbs) to direct the object. A fully functional prototype has been built, tested, and analyzed for changes in the future.

This dissertation contributes to the research in the applied market micro-structure field, aiming to investigate the impact of a specific article of the MiFID II enforced on the 3rd of January 2018: the so-called tick size regime. It is constituted by three papers that see in the market regulators and policy-makers their optimal target. The first paper evaluates the consequences of the new regulation on UK minor venues in terms of liquidity and price discovery and highlights minor unintended consequences in the implementation of the new grid. The second paper builds on these conclusions and promotes an alternative to ESMA grid, a recalibration of the tick size that might lead to a greater orderliness of UK order books. Thethirdpaperendogenouslyinvestigatesthebehaviourofthemarketparticipants in the time frame around the MiFID II enforcement, simulating liquidity breakdowns thus providing the regulators with new simple metrics to detect and monitor abnormal market participants interactions.

The lethal effect of ultraviolet (UV) light on microorganisms is well known and many studies have been undertaken into the effects of UV induced damage. Most of this work has been experimental; by comparison relatively little theoretical work has been undertaken to analyse the kinetics of the UV inactivation process, or to develop quantitative methodologies to support the experimental work. This paper presents a new and simple model for quantifying the photolysis rate. A theoretical study is also presented in this paper which quantifies photolysis rates for E. coli O26 and E. coli O157:H7. This study uses experimental data collected by Tosa and Hirata, and reveals the photolysis rate for E. coil O26 during the UV irradiation process to be 4.69 x 10(-3) m2 J(-1). By comparison, E. coli O157:H7 is much more susceptible to UV induced damage than E. coli O26, having a photolysis constant of only 2.09 x 10(-3) m2 J(-1).

The matrix cracking transverse to loading direction is usually one of the most common observations of damages in composite laminates. The initiation and propagation of transverse cracks have been a longstanding issue in the last few decades. In this paper, a three-dimensional stress analysis method based on the state space approach is used to compute the stresses, including the inter-laminar stresses near transverse cracks in laminated composites. The stress field is then used to estimate the energy release rate, from which the initiation and propagation of transverse cracking are predicted. The proposed method is illustrated by numerical solutions and is validated by available experimental results. To the best knowledge of the authors, the predictions of crack behaviour for non-symmetrical laminates and laminates subject to in-plane shearing are presented for the first time in the literature.

This study is an exploration of the ways in which four Somali students use language to express their identity and assert their views. The study explores the ways in which the Somali students' home culture and the school culture influence the development of their identity. Students participated in a lunchtime focus group on a regular basis over a period of several weeks. Using a micro-ethnographic approach to analysis, the students' interactions were reviewed while considering the ways in which knowledge was affirmed and contested, examples of intertextuality and intercontextuality were identified, and ideational notations or larger world view constructs were pinpointed. In this approach, specific events and interactions were linked to the broader contexts and connections that the participants were using in their communications. The result suggests a new and deeper understanding of the way in which these Somali learners use language to express their identity and negotiate the world. As a result of the examination of their interactions, educators can take from these participants' experiences some ideas about issues to consider when working with second language learners and their families. In this study students used language to assert their own identities as well as to position others in the group. These identities were continually negotiated by students and teachers alike. Students at times pushed back against ways in which they were identified. The Somali learners spoke of changing roles in the family as a result of learning English and being relied on to translate for parents who were non-English speakers. There were occasions where students used language in meaningful and contextually appropriate ways, but without understanding the power of the terms they used. Teachers have a significant role to play in shaping learners use of language and terms and guiding them to a more nuanced understanding of language. By examining children's language, it became apparent that teachers can provide critical information to help parents of second language learners negotiate the school and district resources. Students did express their Somali language and culture as they negotiated their school experience.

<p> Polymers and block copolymers have been grafted from hard and soft polymer micro-spheres by atom transfer radical polymerization (ATRP) and ring opening polymerization (ROP). The hard and soft micro-spheres were prepared by precipitation polymerization of divinylbenzene-80 (DVB80), and of DVB80/hydroxyethylmethacrylate (HEMA), respectively, in neat acetonitrile.</p> <p> Residual vinyl groups in the hard, poly(DVB80) micro-spheres were hydrochlorinated to form benzyl chloride groups that subsequently served as ATRP initiators for poly(styrene) and poly(styrene-block-4-methylstyrene) grafts. Hydrophilic poly(HEMA) and poly(2-( dimethylamino)ethyl methacrylate) poly(DMAEMA) were also grafted from hard micro-spheres containing bromopropionates, using ATRP in the presence of CuBr/Me4Cyclam.</p> <p> Hydroxy groups in soft, poly(DVB80-co-HEMA) micro-spheres were used directly as initiators for ring opening polymerization of ε-caprolactone catalyzed by aluminum compounds. In the addition, they were reacted with α-bromopropionyl bromide to form ATRP initiators.</p> <p> Several combinations of ATRP-ATRP and ROP-ATRP with different monomers were carried out using these initiator micro-spheres. In particular, the soft micro-spheres grafted with poly(methacrylic acid), poly(methylmethacrylate), poly(methylmethacrylate-block-dimethylaminoethylmethacrylate), poly(methylmethacrylate-block-trimethylammoniumethylmethacrylate), poly(methylmethacrylate-block-HEMA), poly(methylmethacrylate-block-glycidylmethacrylate), poly(ε-caprolactone-block- methylmethacrylate), poly(ε-caprolactone-block-dimethylaminoethylmethacrylate).</p> <p> The internal morphology of these homo and block copolymer grafted microspheres was studied using electron microscopy and x-ray microspectroscopy.</p> / Thesis / Doctor of Philosophy (PhD)

The prevention of waterborne diseases requires rapid detection of pathogens in drinking water, with an ultimate goal of in-line monitoring in real time. Standard cultivation-based methods are too time-consuming and thus not suitable for this purpose. Many technologies were proposed to achieve this goal, such as ELISA, PCR, FISH, FTIR and flow cytometry. However, they still have limitations of non-specificity, complexity and high cost. Therefore, an optical system is proposed and developed towards the in-line monitoring of bacteria, which combines the advantages of FTIR and micro-flow cytometer for bacterial identification and precise quantification. The in-line use requires obtaining IR spectra of bacterial cells directly in water, which is achieved using a CaF2 liquid cell. The spectra of a series of bacterial samples are collected and analyzed using principal component analysis for their differentiation. A preliminary study on fabricating a CaF2 concentrator is conducted, in which a novel phenomenon on stress release of silicon nitride film on CaF2 substrate is discovered and studied. To determine the concentration of bacteria in drinking water, a micro-flow cytometer is built based on a micro-fabricated device that integrates on-chip beam-shaping optics and microfluidic channels. With this micro-flow cytometer and optimized data analysis for counting particles in real time, linearity with correlation coefficient of over 0.99 is achieved for the dependences of throughput on both volumetric flow rate and concentration of sample. With a one-dimensional hydrodynamic focusing, no degradation of the counting efficiency is demonstrated when the focused sample stream expands. The high accuracy of counting makes this micro-flow cytometer a promising candidate for low concentration applications. Counting of E. coli DH5α cell suspensions in phosphate buffered saline is performed using the micro-flow cytometer. Side-scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a haemocytometer. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2-4µm) as their scattering intensities are different. / Thesis / Doctor of Philosophy (PhD)

Analyzing the constituents of meteorites has played an important role in forming the contemporary theories of solar system evolution, planets formation, and stellar evolution. Meteorites are often a complex mixture of common rock forming silicates, such as olivines and pyroxenes, with a range of exotic species including hydrated silicates, and in some cases organic compounds. We used Micro-Raman spectroscopy to analyze the compositions of three carbonaceous chondrites: NWA852, Murchison and Allende. Raman spectra were measured using laser sources with different excitation wavelengths: HeNe 633 nm and Nd:YAG 532 nm. We were able to detect 9 minerals in NWA852, 3 minerals in Murchison and 4 minerals in Allende. Some of these minerals like pyrite in NWA852 and magnetite in NWA852 and Murchison provide evidence for potential previous organic life. Other minerals like ringwoodite in Allende and lizardite in NWA852 reveal information about previous astrophysical and geological events experienced by the meteorites. The detection of graphite in the Murchison and Allende reveals information about the microstructure of these meteorites.