There are generally two contrasting alternatives to what limits recruitment in plants, namely the availability of seeds (seed limitation) or the quality or quantity of suitable sites (microsite limitation). Dust seeds, the smallest existing seeds, lack or have minimal nutrient reserves. During germination and initial development they consequently parasitize on mycorrhizal fungi. This is called mycoheterotrophy, and can vary in degree of fungal dependency in adult plants from full, partial or initial mycoheterotrophy. The aim of this thesis was to investigate the recruitment ecology of mycoheterotrophic Ericaceae (tribe Pyroleae) species with dust seeds, and to determine what limits their recruitment. The investigated species were: Chimaphila umbellata, Moneses uniflora, Orthilia secunda, Pyrola chlorantha, P. minor and P. rotundifolia. This aim was achieved by combining field experiments (seed sowing) with isotope analysis and fungal host pyrosequencing. Results provide evidence that the species in Pyroleae are heterogeneous, not only with regard to their degree of mycoheterotrophy, but also concerning germination and early seedling development. A combination of microsite and seed limitation is thus likely to be of importance for all studied species, but the relative importance of these limitations varies among species. Despite having adaptations for wind dispersal the majority of the seeds were deposited in close vicinity of the seed source. But with high seed production at least some seeds should be able to disperse long-distance. Seedlings of all studied species were found to associate with a wide range of ectomycorrhizal fungi, at least during their initial developmental stages. There seems to be a tendency for host narrowing in some Pyroleae species, but not as strict as the host specialization seen in fully mycoheterotrophic Monotropa hypopitys, supporting the hypothesis of geographical and developmental host shifts. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: In press. Paper 4: Manuscript.</p>

Abstract Plant community composition and diversity are determined by the balance between rates of immigration and extinction. Processes of immigration to a local community, i.e. propagule availability and dispersal of propagules between and within habitats, set the upper limit for the pool of species potentially capable of coexisting in a community, while local biotic interactions, i.e., competition, facilitation, herbivory and interactions with below-ground ecosystem components, and environmental factors control colonisation and establishment, and determine the persistence and dynamics of already existing species. In this thesis, I studied (1) the interactions between propagule availability, biotic and environmental constraints on colonisation, and (2) the interdependence between biotic and environmental factors regulating community processes in already established resident vegetation. First, I found that both propagule availability and competition with adult plants limited the rates of colonisation and total community diversity in a relatively low-productive tundra ecosystem. Long-term exclusion of mammalian herbivores and alleviation of nutrient limitation by fertilization increased the intensity of competition with established vegetation, and diminished immigration rates. In addition, I also found that community openness to colonization depended on the initial community properties, i.e., the functional composition and the traits of dominant plants in resident vegetation, which mediate the effects of nutrient addition and biomass removal on immigration rates. Second, adult plants in the resident vegetation experienced an increased extent of neighbourhood competition and herbivory in nutrient enriched conditions and in naturally more fertile habitats. However, the effects were also species-specific. On a community level, release from heavy grazing favoured lichens over graminoids and increased species richness. Furthermore, I also showed that plant community composition was strongly linked with soil organic matter quality and microbial community composition, and that these vegetation-soil-microbe interactions varied along a gradient of soil pH. Overall, my results emphasise that propagule availability, biotic and environmental control over community processes are strongly interconnected in tundra ecosystems. Especially, my findings highlight the role of plant competition and herbivory and their dependence on soil nutrient availability in governing colonisation and resident community dynamics. My results also indicate that plant functional composition and traits of dominant plants are of great importance in channelling community responses to external alterations and dictating plant-soil interactions.