Der Einfluss des Raumcharakters auf die menschliche Tätigkeit ist einer der Grundsätze der „Umwelt-Mensch-Beziehung“ – und findet sich in allen Maßstabsebenen. Zur Beschreibung des Raumcharakters auf Siedlungsmaßstabsebene wird dieser Grundsatz beispielsweise mit den Thesen kognitive Karte (E. Tolman), visuelle Wahrnehmung (J. Gibson), Raumverständlichkeit (K. Lynch) und territoriale Ansprüche (H. Hertzberger) diskutiert. Es wird bisher dargelegt, dass sich das sozialpsychologische Verhalten gewissermaßen auf den Raumcharakter auf Siedlungsmaßstabsebene bezieht. Räume sind also mit ihrer sozialpsychologischen Raumqualität entsprechend zu charakterisieren. Jedoch ist nach heutigem Stand der Siedlungsraumcharakter hinsichtlich der sozialpsychologischen Raumqualität schwierig quantitativ zu bewerten, sodass es mithilfe der empirischen bzw. subjektiven Methode nötig ist, die oben genannten Thesen praktisch zu prüfen. Da diese Arbeit die Position des Apriorismus bzw. der Naturwissenschaft bezieht, wird mithilfe des Werkzeugs Space Syntax versucht, eine apriorische bzw. relativ objektive Methode zur Prüfung dieser Thesen zu entwickeln, um den Siedlungsraumcharakter in die sozialpsychologische Raumqualität umzuwandeln. Durch diese Methode können städtebauliche Maßnahmen zur Lösung der sozialen bzw. sozialpsychologischen Probleme in vorhandenen Siedlungen oder zur Vermeidung dieser Probleme bei Siedlungsneubau vorgeschlagen werden. In dieser Arbeit wird die Methode durch eine Grundtypenanalyse von Siedlungsmorphologien hinsichtlich der jeweiligen sozialpsychologischen Raumqualität musterhaft aufgebaut. Die Stadt Raunheim im Ballungsraum Frankfurt/Rhein-Main, als eine Siedlung in einer wachsenden Metropolregion, wird als Beobachtungsobjekt empirisch untersucht; das Migrationsverhalten dient als sozialpsychologischer Indikator zum Beweisen der Methode.

The extensive reduction in adult Pacific lamprey (Entosphenus tridentatus) counts at many hydroelectric dams in the northwestern USA signals a substantial decline in lamprey numbers across the entire region in the past 40 to 50 years. Among the many potential causes of this decline, obstruction of migration routes has likely played a substantial role. Within the North Umpqua River basin in southwest Oregon, USA, I focused on the following three research goals: 1) to describe the passage efficiency and migration routes of adult Pacific lamprey at Winchester Dam; 2) to evaluate the seasonal movement patterns of adult Pacific lamprey and their use of holding habitat at Winchester Dam in relation to temperature conditions; and 3) to portray the diversity of upstream migratory behaviors of adult Pacific lamprey and the environmental factors that influence these behaviors. This radio telemetry study was conducted between March 2009 and August 2011 with a combination of fixed stations and manual tracking. Passage efficiency was low in both years (8% and 19%, respectively), and all tagged lamprey that successfully passed the dam used routes other than the fish ladder. Lamprey that migrated early within the run and those with relatively small tags had higher passage rates and traveled further than the other groups of lamprey. Lamprey released above of the dam or those that passed the dam on their own distributed themselves widely in the upstream environment, suggesting that the dam deterred their upstream migration. Using mark-recapture data for the two years, the adult Pacific lamprey population upstream of Winchester Dam was estimated at 960 (95% C.I. [188, 4760]) in 2009 and 556 (95% C.I. [110, 2798]) in 2010, which was considerably lower than historical counts at the dam (between 14,532-46,785 in 1965-1971). Most tagged lamprey that did not pass the dam remained at the base of the dam at the end of the summer migration (63% in 2009 and 67% in 2010). Types of habitat most frequently used by lamprey downstream from the dam included the dam surface (wooden structures with crevices), interface zones between fast and slow water, and highway bridge pilings. The lamprey movement changed considerably between August and September, and the frequency of movements decreased sharply during this period. Tagged lamprey were detected using thermal refuges immediately downstream of the dam that were 0.4 to 2.8 C° colder than the mean river temperature at the dam, and this temperature differential increased as the season progressed. Lamprey may be seeking overwintering habitat associated with hyporheic exchange flows at the dam towards the end of the summer season after their display of heightened activity early in the summer. Ninety-five percent of the overall upstream migration took place during the first spring/summer period, and only small-scale upstream movements were observed during the winter and second spring/summer (4% and 1%, respectively). The rate of upstream migration (median) was the fastest during the initial migration phase and was 1.9 km/day (ranging from 0.3 to 11.0 km/day) for tagged lamprey released above Winchester Dam. During winter, 71% of the lamprey remained in the same location where they initiated holding. Multiple regression analysis indicated that the total upstream distance traveled by individual lamprey was most strongly related to presence/absence of Winchester Dam, relative tag size, and water temperature and photoperiod conditions at release. The presence of Winchester Dam, large relative tag size, and high water temperature / short photoperiod conditions at release significantly reduced upstream migration distance. / Graduation date: 2012

Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. This thesis presents work that combines the use of microfluidic devices, optical microscopy and automated image analysis to design various cell biological assays with single cell resolution including cell proliferation, clonal expansion, cell migration, cell-cell interaction and cell viability tracking. In fact, automated high throughput single cell techniques enable new studies in cell biology which are not possible with conventional techniques. In order to automatically track dynamic behavior of single cells, we developed a microwell based device as well as a droplet microfluidic platform. These high throughput microfluidic assays allow automated time-lapse imaging of encapsulated single cells in micro droplets or confined cells inside microwells. Algorithms for automatic quantification of cells in individual microwells and micro droplets are developed and used for the analysis of cell viability and clonal expansion. The automatic counting protocols include several image analysis steps, e.g. segmentation, feature extraction and classification. The automatic quantification results were evaluated by comparing with manual counting and revealed a high success rate. In combination these automatic cell counting protocols and our microfluidic platforms can provide statistical information to better understand behavior of cells at the individual level under various conditions or treatments in vitro exemplified by the analysis of function and regulation of immune cells. Thus, together these tools can be used for developing new cellular imaging assays with resolution at the single cell level. To automatically characterize transient migration behavior of natural killer (NK) cells compartmentalized in microwells, we developed a method for single cell tracking. Time-lapse imaging showed that the NK cells often exhibited periods of high motility, interrupted with periods of slow migration or complete arrest. These transient migration arrest periods (TMAPs) often overlapped with periods of conjugations between NK cells and target cells. Such conjugation periods sometimes led to cell-mediated killing of target cells. Analysis of cytotoxic response of NK cells revealed that a small sub-class of NK cells called serial killers was able to kill several target cells. In order to determine a starting time point for cell-cell interaction, a novel technique based on ultrasound was developed to aggregate NK and target cells into the center of the microwells. Therefore, these assays can be used to automatically and rapidly assess functional and migration behavior of cells to detect differences between health and disease or the influence of drugs. The work presented in this thesis gives good examples of how microfluidic devices combined with automated imaging and image analysis can be helpful to address cell biological questions where single cell resolution is necessary. / <p>QC 20130927</p>

Der Einfluss des Raumcharakters auf die menschliche Tätigkeit ist einer der Grundsätze der „Umwelt-Mensch-Beziehung“ – und findet sich in allen Maßstabsebenen. Zur Beschreibung des Raumcharakters auf Siedlungsmaßstabsebene wird dieser Grundsatz beispielsweise mit den Thesen kognitive Karte (E. Tolman), visuelle Wahrnehmung (J. Gibson), Raumverständlichkeit (K. Lynch) und territoriale Ansprüche (H. Hertzberger) diskutiert. Es wird bisher dargelegt, dass sich das sozialpsychologische Verhalten gewissermaßen auf den Raumcharakter auf Siedlungsmaßstabsebene bezieht. Räume sind also mit ihrer sozialpsychologischen Raumqualität entsprechend zu charakterisieren. Jedoch ist nach heutigem Stand der Siedlungsraumcharakter hinsichtlich der sozialpsychologischen Raumqualität schwierig quantitativ zu bewerten, sodass es mithilfe der empirischen bzw. subjektiven Methode nötig ist, die oben genannten Thesen praktisch zu prüfen. Da diese Arbeit die Position des Apriorismus bzw. der Naturwissenschaft bezieht, wird mithilfe des Werkzeugs Space Syntax versucht, eine apriorische bzw. relativ objektive Methode zur Prüfung dieser Thesen zu entwickeln, um den Siedlungsraumcharakter in die sozialpsychologische Raumqualität umzuwandeln. Durch diese Methode können städtebauliche Maßnahmen zur Lösung der sozialen bzw. sozialpsychologischen Probleme in vorhandenen Siedlungen oder zur Vermeidung dieser Probleme bei Siedlungsneubau vorgeschlagen werden. In dieser Arbeit wird die Methode durch eine Grundtypenanalyse von Siedlungsmorphologien hinsichtlich der jeweiligen sozialpsychologischen Raumqualität musterhaft aufgebaut. Die Stadt Raunheim im Ballungsraum Frankfurt/Rhein-Main, als eine Siedlung in einer wachsenden Metropolregion, wird als Beobachtungsobjekt empirisch untersucht; das Migrationsverhalten dient als sozialpsychologischer Indikator zum Beweisen der Methode.

<p>Effective conservation strategies for sea turtles require knowledge of animal movements and protection of biologically important habitats and life history stages. For breeding adult sea turtles, understanding both their inshore and pelagic spatial patterns is imperative to the successful protection of the species and the accurate identification of their vulnerabilities. This study provides insight into the inter-nesting, post-nesting, and foraging movements of green sea turtles (<i>Chelonia mydas</i>) that nest on Bioko Island, Equatorial Guinea, by using satellite telemetry to track green turtles (n=12) during two nesting seasons (2017-18, 2018-19), and as they migrated to foraging grounds after the nesting season. These tracks were fit with a switching state space model to characterize movements, and then analyzed in relation to environmental and anthropogenic factors. Dive depth data was also used to determine utilization patterns within the water column. The 12 tagged turtles migrated for an average of 1064 km to two distinct foraging grounds, with 10 migrating west for an average of 1115 km to the coastal waters of Ghana, and 2 migrating south for an average of 1563 km to the coastal waters of Angola. Migrating turtles used both direct, pelagic migration strategies, and biphasal, coastal strategies, which included intermittent foraging throughout migrations. Dive depths varied depending on behavior, with an average of 19.3 m during inter-nesting, 12.6 m during migration and 8.5 m during foraging. Knowledge of inter-nesting habitat use, migration patterns, and foraging ground locations will be critical for the development of marine conservation management plans in the Gulf of Guinea and aide in sea turtle conservation efforts throughout the area. Additionally, spatial and dive depth data can inform zonal fishing regulators and provide information needed for modifications to fishing practices and gear that is most likely to reduce sea turtle bycatch. These data will provide a more complete understanding of marine areas critical to sea turtle conservation and aide in sustainable economic development in the Gulf of Guinea.</p><br>