161 |
Molecular characterization of Sulfobacillus and related organismsSchutte, Mart-Alet (Martha Aletta) 03 1900 (has links)
Thesis (MSc)--University of Stellenbosch, 2004. / ENGLISH ABSTRACT: Thirteen Sulfobacillus strains from different geographical locations and two
Alicyclobacillus strains were included in this study. These organisms proved to be
moderately thermophillic (two different sets of optimal temperatures of 45°C and 55°C
were found), Gram-positive, endospore forming bacteria. The pH optima of the strains
tested was pH 2.5 and the pH range lay between pH 1.5 and pH 5.0. It was established
that some strains of Sulfobacillus had the capacity for anaerobic growth when using ferric
iron as an electron donor. It was determined that S. thermosuljidooxidans was the species
found within South African biooxidation plants. Plasm ids were identified within strain
611 (S. thermosuljidooxidans) isolated from a Billiton commercial plant.
The sample of Sulfobacillus strains used in this study could clearly be divided into two
groups based on the analysis of their 16S rRNA gene sequences as well as the number of
ribosomal (rm) operons present as determined by Southern hybridization.
A system for the convenient identification of Sulfobacillus species was developed using
several of the techniques employed in this study. Preliminary identifications can be
obtained by restriction enzyme digestion of the PCR amplified 16S rRNA gene.
Confirmation of this placement can be done by comparison of the 16S - 23S rRNA
spacer region amplification band sizes. Once the preliminary identification has been
completed it is possible to place the isolate in the correct species by making use of the
differences in sugar utilization that the species exhibit. The more laborious method of
16S rRNA sequence comparisons can be undertaken if there is still any uncertainty as to
which species an isolate belongs to. Phylogenetic results obtained from the 16S rRNA gene sequence indicates that the genus
Sulfobacillus should probably be divided into two individual genera. Further information
gathered from the phylogenetic comparisons indicates that strain Riv-14 previously
assigned to S. ambivalens is more closely related to S. montseratensis. Data obtained
from 16S - 23S rRNA spacer region analysis confirms this result.
Future work includes the use of DNA-DNA hybridization studies and mol% G+C ratio's
in order verify the presence of two distinct genera as well as placing Riv-14 within the
correct species. / AFRIKAANSE OPSOMMING: Dertien isolate van die genus Sulfobacillus afkomstig van geografies verskillende areas
en twee isolate van die genus Alicyclobacillus is in die studie ingesluit. Hierdie
organismes het gewys dat hulle gematigde termofiele (twee verskillende groepe met
optimale temperature van 45°C en 50°C elk was waargeneem), Gram-positiewe,
endospoorvorrnende organismes is. Die pH optima van die isolate was pH 2.5 en die
reeks van pH waar groei moontlik was het tussen pH l.5 en pH 5.0 gelê. Dit was bewys
dat sekere van die Sulfobacillus isolate oor die vermoë beskik het om anaerobies te
respireer deur ferri yster (Fe3+) as elektron akseptor te gebuik. Dit was bepaal dat S.
thermosulfidooxidans die spesies is wat teenwoordig was in die bio-oksidasie reaktors in
Suid Afrika. Plasmiede vanuit die isolaat 611 (s. thermosulfidooxidans) afkomstig vanuit
'n Billiton komersieële reaktor, is geidentifiseer.
Die toetsmonster van Sulfobacillus isolate gebruik in hierdie studie het duidelik daarop
gewys dat daar twee groepe binne Sulfobacillus is. Hierdie stelling is gebaseer op data
afkomstig van die analiese van die 16S rRNA volgorde asook die aantal ribosomale (rm)
kopieë teenwoordig soos bepaal deur Southern klad eksperimente.
'n Sisteem vir die maklike identifikasie van Sulfobacillus spesies is ontwerp deur van
verskeie tegnieke, soos in hierdie studie toegepas, gebruik te maak. Aanvanklike
identifikasie kan verkry word deur gebruik te maak van restriksie ensiem vertering van
PKR geamplifiseerde 16S rRNA geen. Hierdie plasing van die isolaat kan bevestig word
deur die grootte van die 16S - 23S rRNA intergeniese amplifikasie produkte te vergelyk.
Sodra die aanvanklike plasing van die isolaat voltrek is, kan daar van die verskille in die
vermoëns van die spesies om sekere suikers the benut, gebruik gemaak word om die
isolaat binne die regte spesies te plaas. Die meer werksintensiewe metode van 16S rRNA
volgorde vergelyking kan gebruik word indien daar enige onsekerheid is oor by watter
spesies die isolaat hoort. Filogenetiese resultate verkry van die vergelyking van die 16S rRNA geen volgorde dui
daarop aan dat die genus Sulfobacillus waarskynlik uit meer as een genus bestaan. Die
filogenetiese data dui verder daarop dat die isolaat Riv-14 wat as 'n S. ambivalens
geklassifiseer is, nader verwant is aan die spesies S. montseratensis. Data verkry vanaf
die 16S - 23S intergeniese gebied analiese bevestig hierdie resultaat.
Toekomstige werk sluit DNA-DNA hibridisasie en mol% Gte ratio eksperimente in om
sodoende die teenwoordigheid van meer as een genus sowel as die plasing van Riv-14 in
die korrekte spesies te bevestig.
|
162 |
Molecular analyses of candidate carotenoid biosynthetic genes in Vitis vinifera L.Young, Philip Richard, 1973- 03 1900 (has links)
Thesis (PhD)--University of Stellenbosch, 2004. / ENGLISH ABSTRACT: Plants cannot avoid stress and must therefore be capable of rapidly responding to
extreme environmental changes. An inability to control and regulate the
photosynthetic process during stress conditions will lead to the formation of highly
reactive oxygen species that concomitantly causes photo-oxidative damage to the
pigments and proteins of the photosynthetic apparatus. Since light is the primary
source of energy for the photosynthetic process, it is clear that plants are
continuously required to balance the light energy absorbed for the photochemical
reactions against photoprotection in a dynamic way in order to survive. Carotenoids
are precursors of abscisic acid, but more importantly structural components of the
photosynthetic apparatus. During photosynthesis carotenoids function as accessory
light-harvesting pigments, and also fulfil a photoprotective function by quenching the
reactive molecules formed during conditions that saturate the photosynthetic process.
Due to the importance of carotenoids to plant fitness and human health (as
Vitamin A precursors) this study has attempted to isolate and characterise genes that
are directly, or indirectly involved in carotenoid biosynthesis in Vitis vinifera. In total
eleven full-Iength- and eight partial genes have been isolated, cloned and
sequenced. These genes can be grouped into the following pathways: (i) the 1-
deoxy-D-xylulose 5-phosphate (DOXP)/2-C-methyl-D-erythritol 4-phosphate (MEP)
pathway (i.e. the plastidic isopentenyl diphosphate biosynthetic pathway); (ii) the
mevalonate pathway (i.e. the cytosolic/mitochondrial IPP biosynthetic pathway); (iii)
the carotenoid biosynthetic pathway; (iv) the abscisic acid biosynthetic pathway (as a
degradation product of carotenoids); and general isoprenoid biosynthetic pathways
(as precursors of carotenoids).
The full-length genes (i.e. from the putative ATG to the STOP codon) of DOXP
synthase (DXS), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (lytB), IPP
isomerase (IPI), 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS), phytoene
synthase (PSY), Iycopene ~-cyclase (LBCY), ~-carotene hydroxylase (BCH),
zeaxanthin epoxidase (lEP), 9-cis-epoxy carotenoid dioxygenase (NCED), farnesyl
diphosphate synthase (FPS) and geranylgeranyl diphosphate synthase (GGPS) have
been isolated from cDNA. In addition, the full-length genomic copy and putative
promoters of DXS, PSY, LBCY, BCH, NCED and lEP have also been isolated from
genomic DNA by the construction and screening of sub-genomic libraries.
Alignments of the genomic copies of these genes to the corresponding cDNA
sequences have provided useful information regarding the genomic organisation of
these genes, including the intron-exon junction sites in V. vinifera. The copy number
of the DXS, PSY, LBCY, BCH, NCED and lEP encoding genes in the Vitis genome
have been determined. DXS, PSY, BCH and lEP are single copy genes, whereas
LBCY and NCED have two and three copies, respectively.
The transcriptional activity of the putative promoters of six of the isolated genes
(i.e. DXS, PSY, LBCY, BCH, lEP and NCED) were tested with a transient reporter
gene assay. None of the putative promoters tested showed any transcriptional
activity of the reporter gene. The transcription of these genes, has however been
shown using northern blot analysis and/or RT-PCR. Preliminary expression profiles
for PSY, LBCY, BCH, and lEP were determined in different plant organs and the
expression of these genes was generally higher in photosynthetically active tissues.
The expression of these genes following different treatments (abscisic acid, NaCI and
wounding) was also assayed. The functionality of five of the isolated full-length genes (IPI, GGPS, PSY, LBCY and BCH) has been shown in a bacterial colour
complementation assay.
In silica analysis of the predicted protein sequences of all eleven isolated
genes revealed that they are conserved and share a high degree of homology to the
corresponding proteins in other plant species. The sequences were further analysed
for conserved domains in the protein sequences, and these proteins typically
demonstrated similar domain profiles to homologues in other species (plant, bacteria
and algae). The predicted protein sequences were further analysed for transit
peptides, the presence of which would provide evidence for the sub-cellular
localisation of the mature peptides. Since these genes are involved in biosynthetic
pathways that are active in discrete organelles, the sub-cellular localisation of most of
these proteins is known. The carotenoid biosynthetic genes (PSY, LBCY, BCH and
ZEP), the abscisic acid biosynthetic gene, NCED, as well as the DOXP/MEP pathway
genes (DXS, lytB and IPI) were all localised to the chloroplast. The mevalonate
pathway gene, HMGS, was localised to both the cytosol and the mitochondria, and
the general isoprenoid precursor genes, FPS and GGPS, were localised to the
cytosol and the chloroplast, respectively. All these results are in agreement with the
localisation of the respective pathways.
In order to increase our understanding of carotenoid biosynthesis and functions
in plants, we constitutively overexpressed one of the isolated genes (BCH) in the
model plant, Nicotiana tabacum. Plants expressing the BCH gene in the sense
orientation maintained a healthy photosynthetic rate under stress conditions that
typically caused photoinhibition and photodamage in the untransformed control
plants. This result was inferred using chlorophyll fluorescence and confirmed using
CO2 assimilation rates and stomatal conductance. Chlorophyll fluorescence
measurements indicated that the photo protective non-photochemical quenching
ability of the BCH-expressing plants increased, enabling the plants to maintain
photosynthesis under conditions that elicited a stress response in the untransformed
control plants. An integral photosynthetic protein component, the D1 protein, was
specifically protected by the additional zeaxanthin in the BCH sense plants. Plants
expressing an antisense BCH proved the converse, i.e. lower levels of BCH resulted
in decreased zeaxanthin levels and made the transgenic plants more susceptible to
high-light induced stress. These results have shown the crucial role of carotenoids
(specifically the xanthophylls) in the photoprotective mechanism in plants. The
increased photoprotection provided by the BCH expressing plants suggests that the
scenario in plants is not optimal and can be improved. Any improvement in the
photoprotective ability of a plant will affect both the fitness and productivity of the
plant as a whole and will therefore find application in a number of crop plants on a
global scale. This study has resulted in the successful isolation and characterisation
of genes involved in the direct, or indirect, carotenoid biosynthetic pathways. The
further study and manipulation of these genes in model plants will provide useful
insights into the physiological role of specific carotenoids in photosynthesis and in
plants as a whole. / AFRIKAANSE OPSOMMING: Plante het nie die vermoë om stres te ontwyk nie en moet dus vinnig op veranderinge
in hulomgewingstoestande kan reageer. Indien hulle nie die fotosinteseproses kan
kontroleer en reguleer tydens streskondisies nie, sal dit tot die vorming van hoogs
reaktiewe suurstofspesies lei, wat beide die pigmente en proteiene van die
fotosintetiese apparaat sal beskadig. Lig is die primêre energiebron vir fotosintese
en daarom is dit noodsaaklik dat plante deurgaans 'n dinamiese balans tussen
fotosintese en fotobeskerming moet handhaaf. Karotenoiëde is voorlopers vir die
vorming van absisiensuur, maar meer belangrik vir die plant, ook integrale
komponente van die fotosintetiese apparaat. Tydens fotosintese word karotenoiëde
vir die opneem van lig benodig, terwyl dit ook die fotosintetiese apparaat beskerm
wanneer lig 'n versadigingspunt bereik vir fotosintese.
Weens die belang van karotenoiëde vir plant- en menslike gesondheid (as
Vitamiene A voorlopers), het hierdie studie beoog om gene te isoleer en
karakteriseer wat direk of indirek 'n rol in karoteenbiosintese in Vitis vinifera speel.
Elf vollengte- en agt gedeeltelike gene is geïsoleer, gekloneer, en gekarakteriseer.
Hierdie gene kan in die volgende biosintetiese paaie gegroepeer word: (i) die 1-
deoksi-D-xilulose 5-fosfaat (DOXP)/2-C-metiel-D-eritritol-4-fosfaat (MEP) pad (d.w.s.
die plastiediese isopenteniel difosfaat biosintetiese pad); (ii) die mevalonaat pad
(d.w.s. the sitosoliese/mitokondriale IPP biosintetiese pad); (iii) die karotenoiëd
biosintetiese pad; (iv) die absisiensuur biosintetiese pad (as 'n afbraak produk van
karotenoiëde) en die algemene isoprenoïed bisintetiese paaie (as voorlopers van
karotenoiëde ).
Die vollengte gene (d.w.s. vanaf die geskatte ATG tot die STOP kodon) van
DOXP-sintase (DXS), 4-hidroksi-3-metielbut-2-eniel difosfaatreduktase (lytB), IPPisomerase
(IPI), 3-hidroksi-3-metielglutariel koensiem A sintase (HMGS), fitoeën
sintase (PSY), likopeen p-siklase (LBCY), p-karoteen hidroksilase (BCH), zeaxantien
oksidase (ZEP), 9-cis-epoksi karotenoiëd dioksigenase (NCED), farnesiel difosfaat
sintase (FPS)en geranielgeraniel difosfaat sintase (GGPS) is met behulp van. RTPKR
vanaf eDNA geïsoleer. Die vollengte genomiese kopieë en die verwagte
promotors van die DXS, PSY, LBCY, BCH, NCED and ZEP gene is ook geïsoleer
d.m.v. die opstel en sifting van subgenomiese biblioteke. Vergelykende analises van
die genoom- en eDNA kopieë het insiggewende data oor die genomiese rangskikking
van die gene, insluitende die intron-ekson setels in V. vinifera gelewer. Die
kopiegetalle van DXS, PSY, LBCY, BCH, NCED en ZEP is bepaal. DXS, PSY, BCH
en ZEP is in die Vitis-genoom as enkel kopieë teenwoordig, terwyl LBCYen NCED
twee en drie kopieë, repektiewelik, beslaan.
Die transkipsionele aktiwiteit van die verwagte promotors van ses van die
geïsoleerde gene (naamlik DXS, PSY, LBCY, BCH, ZEP en NCED) is d.m.v. 'n
tydelike verklikkergeentoets ondersoek. Geeneen van die promotors het die
transkripsie van die verklikkergeen bemiddel nie. Die transkripsie van die gene is
egter wel bewys deur van northernhibridisasies en/of RT-PKR gebruik te maak. Die
promotors van hierdie gene kan dus as transkipsioneel aktief beskou word.
Voorlopige uitdrukkingsprofiele van PSY, LBCY, BCH, en ZEP is in verskillende
plantorgane bepaal; die profiele was deurgaans hoër in fotosinteties aktiewe
weefsels. Die uitdrukkingsprofiele van die gene is verder ook in reaksie op
verskillende induktiewe behandelings (absisiensuur, NaCI en beskadiging) bepaal. Vyf van die vollengte gene (IPI, GGPS, PSY, LBCYen BCH) is funksioneel bewys in
'n bakteriese funksionele kleurkomplementasiesisteem.
In silico analises van die afgeleide proteïene van al elf geïsoleerde gene het 'n
hoë vlak van homologie met ooreenstemende proteiene van ander plantspesies
getoon. Gekonserveerde domeine is ook in die proteïensekwense van die
geïsoleerde gene teenwoordig. Hierdie proteïene het deurgaans dieselfde
domeinprofiele vertoontoon as homoloë in ander spesies (bakterieë, alge en plante).
Die sub-sellulêre teikening van die gene kon voorspel word deur die seinpeptiede in
die proteiensekwense te eien. Aangesien hierdie gene betrokke is by biosintetiese
paaie wat in diskrete kompartemente plaasvind; is die sub-selluiêre lokalisering van
hierdie proteïene voorspelbaar. Die karotenoïed biosintetiese gene (PSY, LBCY,
BCH en ZEP), die absisiensuur biosintetiese geen, NCED, sowel as die DOXP/MEP
pad se gene (DXS, lytB en IPI) kom almal in die chloroplast voor. Die
mevalonaatpadgeen, HMGS, word na beide die sitosol en die mitokondria geteiken,
terwyl die algemene isoprenoïed voorlopergene, FPS en GGPS, onderskeidelik na
die sitosol en die chloroplast geteiken word. Die verkreë voorspellings stem met die
lokalisering van die biosintetiese paaie in die selooreen.
Om ons kennis rakende karotenoïed biosintese en veral hulle funksie(s) in
plante te verbreed, het ons een van die geïsoleerde gene, BCH, in die model plant,
Nicotiana tabacum, konstitutief ooruitgedruk. Plante wat die BCH geen in die "sense"
orientasie uitgedruk het, kon normale fotosintetiese aktiwiteit handhaaf onder
kondisies wat foto-inhibisie en foto-osidatiewe skade in die ongetransformeerde
kontrole plante veroorsaak het. Hierdie resultaat is met chlorofil fluoresensie
analises aangetoon terwyl dit met CO2 assimilasie- en huidmondjie
geleidingseksperimente bevestig is. Chlorofil fluoresensie metings het aangetoon
dat die beskermingsvermoë van die transgeniese plante verhoog is, en dit dan die
plante in staat stelom fotosintetese te handhaaf onder streskondisies van hoë lig.
Proteïen analises het aangetoon dat 'n integrale fotosintetiese proteien, die 01
proteïen, word veral deur die verhoogde zeaxantien vlakke in die BCH transgeniese
plante beskerm. Plante wat verminderde zeaxantien vlakke gehad het, weens die
konstitutiewe ooruitdrukking van die BCH geen in die anti-"sense" orientasie, het die
teenoorgestelde bewys. Met ander woorde. laer BCH vlakke (en dus laer zeaxantien
vlakke) het tot plante wat meer vatbaar was vir hoë lig geïnduseerde stress gelei.
Hierdie resultate het die essensiële beskermende rol wat karotenoiede tydens
fotosintese speel, uitgelig. Die vermoë om hierdie beskermende meganisme te
manipuleer in transgenies plante het aangetoon dat die sisteem in plante, alhoewel
effektief, nie optimaal is nie. Enige verbetering in 'n plant se inherente vermoë om
streskondisies te weerstaan sal die plant se algemene gesondheid en dus
produktiwiteit beïnvloed. As sulks sal hierdie in meeste gewasspesies toepassing
vind. Hierdie studie beskryf die isolering en karakterisering van gene wat direk, of
indirek, by karotenoïedbiosintese betrokke is. Verdere studies, en veral die
manipulering van hierdie gene in model plante, sal die fisiologiese rol van spesifieke
karotenoïeede in fotosintese, en die plant as 'n geheel, ontrafel.
|
163 |
Molecular systematic study of Southern African Oxalis (Oxalidaceae)Oberlander, Kenneth Carl 03 1900 (has links)
Thesis (PhD (Botany and Zoology))—University of Stellenbosch, 2009. / The genus Oxalis forms a major part of the flora of southern Africa, in particular the
Cape Floristic Region (CFR) at the southwestern tip of the continent, but the current
taxonomy is outdated and ecological knowledge of the lineage is sadly incomplete. In
this thesis I set out to address several aspects of Oxalis systematics that urgently
require attention.
Firstly, the current macro-morphological taxonomy requires phylogenetic testing, as it
is acknowledged to be incomplete and artificial. I address this need by providing a
DNA sequence-based phylogeny of three markers, using three different inference
methods, for nearly three quarters of the indigenous species. This phylogeny
confirmed both the monophyly of the southern African taxa, and the artificiality of the
current classification system. It is congruent with previous sequence-based
reconstructions of smaller groups of southern African Oxalis species, and with the
palynological classification proposed for the genus.
Secondly, previous phylogenetic work on the southern African members could not
resolve basal relationships within the southern African clade. I attempt to address this
problem by sequencing three extra chloroplast markers for a select group of taxa,
followed by separate and combined (total evidence) molecular phylogenetic analyses.
This approach did increase resolution at the base of the southern African lineage, but
many clades still showed poor resolution and support despite the use of more than 7
000 bases of sequence data. Resolving these clades within the southern African Oxalis
phylogeny remains a challenge, and should prove a fertile field for future research.
Thirdly, the ages (and thus duration of presence) of many Cape plant lineages within
the CFR are of major interest, given that the CFR represents a global biodiversity
hotspot. The age of the genus in the Cape is estimated by analyzing combined
sequence data for all sampled taxa under both a Bayesian Relaxed Clock and a semiparametric
Penalised Likelihood method, using calibration points inferred from
Relaxed Clock analyses of the entire order Oxalidales, for which fossil data are
available. In an attempt to account for known problems with divergence time
estimation, I explored the potential bias introduced by method used, marker genome
source and different calibrations on the root. The results indicate substantial variation
in the age of crown southern African Oxalis over a nearly twenty million year period,
varying according to source data, calibration estimate and methodology employed in
the reconstruction. Despite this major variability, all average estimates are older than
iv
18 million years, which agrees with a growing body of evidence that there has been a
gradual accumulation of floristic diversity in the CFR, rather than a rapid, recent burst
of speciation.
Fourthly, as the produced phylogenies conclusively show the artificial nature of the
current taxonomy, I propose a new, almost completely different classification for
southern African Oxalis taxa. Although a significant improvement, this classification
is considered informal due to the complete disagreement between the old and
proposed new taxonomies, poor resolution in some of the proposed lineages, and a
need to confirm proposed groups (clades) with the identification of morphological
synapomorphies. Potential synapomorphies for various clades are proposed and
discussed, which should guide future research.
Fifthly, the presence of bulbs in this genus is of great interest as a potential preadaptation
for seasonally arid climates. The evolution of the bulbous habit in Oxalis is
here explored for the first time. I address the sequence of major morphological
character state changes leading to the suite of characters corresponding to the bulbous
habit. The homology of basal leaf petioles, fleshy leaf scales and tunics is discussed,
and it is shown that many bulb characters present in the southern African lineage are
also found in the close relatives of this lineage, and are thus older than this lineage.
The ecological and evolutionary implications of bulb geophytism in the CFR Oxalis
are also discussed.
Finally, I address certain taxonomic issues that arose during the course of this study.
Co-authors and I describe the new species O. saltusbelli and O. ericifolia. We also
clarify issues surrounding the tremendously variable group species O. flava and
propose some nomenclatural changes and synonyms for related taxa. We also address
the taxonomic position of the rare species O. purpurata, which was located too late in
the course of this study to include in the main analyses.
|
164 |
Bond selective electronic excitation and short-time photodissociation dynamics of dihalomethanes from resonance raman spectroscopy郭偉明, Kwok, Wai-ming. January 1997 (has links)
published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy
|
165 |
Freshwater fungi: biodiversity, origins and molecular taxonomyDhanasekaran, Vijaykrishna. January 2005 (has links)
published_or_final_version / abstract / Ecology and Biodiversity / Doctoral / Doctor of Philosophy
|
166 |
Genetics and molecular characterization of degenerative disc diseaseJim, Jin-to., 詹展韜. January 2005 (has links)
published_or_final_version / abstract / Orthopaedics and Traumatology / Doctoral / Doctor of Philosophy
|
167 |
Signaling pathways modulated by gold-1A in its anti-tumour effects against hepatocellular carcinomaLi, Hoi-yee., 李凱怡. January 2006 (has links)
published_or_final_version / abstract / Chemistry / Master / Master of Philosophy
|
168 |
Role of AMP-activated protein kinase in cervical cancer cell growthYu, Yee-man., 余綺雯. January 2006 (has links)
published_or_final_version / abstract / Obstetrics and Gynaecology / Master / Master of Philosophy
|
169 |
Molecular changes in regenerated and senescent cultured endothelial cellsLee, Yuk-kwan, Mary., 李玉筠. January 2006 (has links)
published_or_final_version / abstract / Pharmacology / Doctoral / Doctor of Philosophy
|
170 |
The significance of proline rich tyrosine kinase 2 (PYK2) in proliferation and invasiveness of hepatocellular carcinomaSun, Kin-wai., 孫建維. January 2008 (has links)
published_or_final_version / Surgery / Doctoral / Doctor of Philosophy
|
Page generated in 0.0895 seconds