CAPES / Honey is generally known for its therapeutic value and the determination of its main floral source allows the certification to the consumer the properties related to its origin. Thus, the aim of this work was to evaluate the quality and characterize eleven honeys from Apis mellifera from the state of Rio de Janeiro according to physico-chemical parameters, to phenolics profile, to the antioxidant activity and also through the use of chemonetric analysis applied to 1H NMR data and HPLC-DAD. Two samples of assa peixe, six samples of cambara and three samples of morr?o-de-candeia honeys from diferente regions of Rio de Janeiro were analyzed. The physico-chemical parameters determined were: HMF content and color through a spectrophotometric method, free acidity and pH. The content of free amminoacids was also determined through the c?dmium-ninhydrine together with total proteins via the Bradford method. The antioxidant ability of honeys and their extracts was qualitatively determined through the total phenolics content using the Folin-Denis method. Total flavonoids were determined by the complexation method with aluminium chloride. The quantitative antioxidant activity of honeys was determined by the trapping of the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical, by trapping of the ABTS.+ radical and also by the iron reduction method (FRAP). The identification and quantitation of polyphenols in the extracts were done by HPLC-PDA. The use of multivariate analysis for the 1H NMR data and HPLC enabled the distinction of the honeys analyzed in this work. Thus, the use of 1H NMR and HPLC data combined with multivariate analysis may be employed as a new strategy for the fast and non-destructive typification of Brazilian honeys. / No mercado, m?is s?o conhecidos pelo seu poder terap?utico e a designa??o de sua principal fonte floral permite atestar ao consumidor as propriedades de sua origem. Assim, o objetivo deste trabalho foi avaliar a qualidade e caracterizar onze m?is de Apis mellifera provenientes do Estado do Rio de Janeiro, por meio de par?metros f?sico-qu?micos, do perfil de subst?ncias fen?licas, de seus potenciais antioxidantes, al?m de usar m?todos quimiom?tricos aplicados aos dados de RMN de 1H e CLAE-DAD. Para tanto, utilizou-se duas amostras de mel de assa peixe, seis de cambar? e tr?s de morr?o de candeia de diferentes munic?pios do Rio de Janeiro. Os par?metros f?sico-qu?micos avaliados foram: teor de HMF e a cor utilizando m?todo espectrofotom?trico, acidez livre e pH. Al?m dessas determina??es, o conte?do de amino?cidos livre foi avaliado pelo m?todo de c?dmio-ninidrina, e prote?nas totais pelo m?todo de Bradford. A capacidade antioxidante dos m?is e de seus extratos foi avaliada qualitativamente atrav?s do conte?do de fen?licos total pelo m?todo de Folin-Denis, e de flavon?ides total pelo m?todo de complexa??o com cloreto de alum?nio. A quantifica??o do potencial antioxidante foi realizada pela captura do radical 2,2-difenil-1-picril-hidrazil (DPPH), captura do radical livre ABTS.+, al?m do m?todo de redu??o do ?on f?rrico (FRAP). A identifica??o e quantifica??o das subst?ncias polifen?licas dos extratos foi feita por cromatografia l?quida de alta efici?ncia com detector de arranjo de diodos (CLAE-DAD). A aplica??o da an?lise multivariada aos dados de RMN de H e de CLAE-DAD distinguiu os m?is de assa peixe, cambar? e morr?o de candeia produzidos no Estado do Rio de Janeiro. Assim, o uso de RMN de 1H e CLAE-DAD combinado com a quimiometria pode ser uma nova estrat?gia para tipifica??o de m?is brasileiros de forma r?pida e n?o destrutiva.

Honey has been used to heal wounds since ancient times. There are many references in ancient literature that cite honey for its medicinal uses. It is used as an alternative agent to cure infections of wounds, burns, ulcers etc. Researchers have shown some of the antimicrobial properties of honey when used as an ointment. When applied to an affected area, it helps to promote the growth of healthy tissue. One of the factors on which the quality of the honey depends, is its geographical origin. Based on the location, honey types can vary as much as 100-fold from each other in color, aroma, viscosity, and antimicrobial properties. The important components in honey that play an essential part in healing wounds and contributing to the antimicrobial properties are enzymes. Their presence allows honey to kill various types of pathogenic bacteria, viruses, fungi etc. A higher antimicrobial effect is seen in monofloral honey (when a single plant species is the source of nectar), which is often more potent than other types of honey in terms of antibacterial activity. Resistance of pathogens to these antimicrobial actions has never been shown, which makes honey a more promising source of antimicrobial research. Presently, infections of burns and wounds are very challenging to treat, especially when they are caused by antibiotic-resistant bacteria. The purpose of this study was to examine the antimicrobial properties of honey from Utah and other locales, and to identify promising antimicrobial activities that could be useful in treating infections caused by resistant bacteria.