Metabolomics is a discipline where metabolites are assessed, identified and quantified in different samples. Metabolites are crucial components of the biological system and highly informative about its functional state due to the closeness to functional endpoints and to the organism’s phenotypes. 1H NMR and LC-MS, the commonly used metabolomics analytical platforms were used to annotate the metabolites found in Sclerocarya birrea (S. birrea) leaves from five South African provinces, Limpopo (L), Gauteng (G), North West (NW), Mpumalanga (M) and KwaZulu-Natal (KZN). Supervised Orthogonal Partial Least Square – Discriminant Analysis (OPLS-DA) of the full spectra revealed a clear differentiation of S. birrea leaves from five provinces. In addition, the level of common metabolites were measured and compounds previously found to have anti-diabetes potential ((-)-epicatechin 3-0-galloyl ester, myricetin-3-0-α-L-rhamnopyranoside, gallic acid and Kaempferol-3-0-α-L-rhamnopyranoside) were annotated in the samples. The samples from the five provinces showed anti- diabetic activity when exposed to an in-vitro glucose uptake assay, with the highest activity observed in male samples from M. The sample presented high concentrations of (-)-epicathechin 3-0-galloyl ester, one of the metabolites with anti-diabetes activity. Overall 1H NMR and LC-MS metabolic profiling were successfully applied to discriminate all five sources of S. birrea leaves, and obtained qualitative information of many common metabolites / Agriculture, Animal Health and Human Ecology / M. Sc. (Agriculture)

Despite attempts by the South African government in partnership with private sector to equip schools with computers, research has shown that technology integration into teaching and learning is very low. Literature revealed that professional development or capacitation of teachers as the key element in enabling them to utilise technology and integrate it in their teaching practices. The main aim of this study was to design and develop Information Communication and Technology (ICT) instructional tools which were to be used to train teachers in primary schools on how to integrate ICT into teaching and learning. Design research and Instructional design theories were employed in guiding this study. The study used the ADDIE model of Instructional design as a guiding principle in the design and development of the tools. The study is located in the positivist paradigm of exploring reality and the methodology employed in this study was quantitative in nature. The participants were 28 teachers from three primary schools. The sample was purposely selected since the schools were awarded computers after taking part in a water management project. The quantitative data to determine the participants‟ prior knowledge, effectiveness of the solution and the teachers‟ satisfaction with the designed solution were collected by means of two questionnaires administered in two phases: pre- and post- workshop. The results of this study revealed that capacitation of teachers have to lay the foundation for ICT integration into teaching and learning. Giving computers to schools can lead to successful integration once the teachers feel competent to use them. Furthermore, it is crucial that other intervention strategies be explored, to empower teachers. It is recommended that this study be transferred to other similar situations. / Curriculum and Instructional Studies / M. Ed. (Curriculum Studies)

The aim of the dissertation is to indicate that a large number of problems in rural communities are the consequence of ineffective knowledge management, and that the effective management of knowledge could significantly improve the range and quality of services provided to community members. Knowledge is reviewed by examining the process of changing social facts into data, data into information and information into knowledge. Knowledge management is examined by reviewing the history of knowledge management as well as defining the term knowledge management. A generic model for knowledge management is developed that divides knowledge management into five basic processes namely; knowledge identification, knowledge mobilisation, knowledge generation/elaboration, knowledge application and knowledge evaluation. The model further describes the knowledge management ”enablers” that can either facilitate or debilitate the management of knowledge. Various practical suggestions are proposed that will facilitate the implementation of a knowledge management programme in a rural community. / Psychology / MA (PSYCHOLOGY)

M.Sc. / Pollution of the earth is presently one of the most important environmental issues of the world and various attempts, including creation of public awareness have been initiated over the past few years to address this situation. There is especially a global concern about the progressive pollution of valuable freshwater systems which most organisms, including humans, are reliant upon. This includes South Africa's aquatic ecosystems, many of which have been degraded over the past few decades. The Olifants River in Mpumalanga is one of the most threatened river systems in South Africa. It is impacted by various anthropogenic activities, particularly mining in the upper catchment, and urbanisation, industrial and agricultural activities in the upper and lower catchments. Previous investigations have shown that these activities are responsible for the degradation of this river system. The broad objective of this study was to obtain data on water and sediment quality, as well as bioaccumulation of metals in fish via monitoring of the Olifants River. It formed part of a major study concerned with the experimental investigation of lethal and sub-lethal effects of metals on fish physiology, supported by a field investigation into the water quality and metal contamination of the biotic and abiotic components of the Olifants River system. Physico-chemical properties of the water as well as the concentrations of some metals (Al, Fe, Cu, Zn, Ni, Mn, Pb & Cr) in the water and sediment were seasonally investigated. The extent of bioaccumulation of these metals in selected organs/tissues (muscle, gills, liver & skin) of Oreochromis mossambicus and Clarias gariepinus from Loskop Dam (upper catchment) and Mamba Weir, Kruger National Park (lower catchment) was also investigated. High levels of various macro-constituents and metals were detected at many sites in the study area and in many cases they exceeded the guideline limits set for aquatic ecosystems. Localities 2, 3, 6, 8, 10, 12 and 17 were observed to be severely impacted (see Chapter 9- Table 9.1) by elevated concentrations of pollutants influencing variables such as TDS and sulphates. This confirms that these sites are being impacted by mining. This was further confirmed by low pH-values at localities 3, 5, 8, 9, 10 and 12 which indicate on acid mine drainage originating from the many coal mines in the upper catchment of the Olifants River. Nutrient enrichment (elevated levels of phosphates, nitrates and nitrites) occurred at many sites in the catchment but in particular at localities 3, 4, 6, 10, 11, 14, 15 and 17. Point source pollution from sewage treatment works and non-point sources from agricultural runoff and informal settlements are the main contributors to these elevated levels of nutrients. The Selati River, impacted in particular by elevated phosphate levels, is the main contributor to the high nutrient levels detected at locality 17. It is evident from the evaluation of the metal concentrations in the water and sediment (Table 9.2) that most of the sites in the Olifants River catchment are being impacted by metal pollution. Oreochromis mossamhicus and C gariepinus sampled at selected sites in the Olifants River accumulated selected metals in the following order; Fe>Zn>Al>Cr>Ni>Pb>Mn>Cu. Levels were generally high in the liver and gills, while much lower levels were detected in the skin and muscle tissues. Gill tissue is in direct contact with the aqueous environment and therefore gives a good indication of the extent of exposure. Skin tissues usually contained relatively low levels of metals (except for Zn). Although muscle tissues usually accumulated low metal concentrations it is an important tissue to monitor in bioaccumulation studies as it can lead to metal poisoning if contaminated muscle tissues are consumed by humans. Adult specimens were mostly sampled and within this range there was a slight decrease in Cu, Al, Fe, Ni, Mn and Cr concentrations with increasing age. Temporal variation in metal accumulation by fish possibly occurred due to variations in metal concentrations in the water and sediment at a locality. These differences were caused by seasonal variation in climatical conditions (eg. rainfall, temperature), as well as temporal fluctuations of pollutant inputs into the river system. Aquatic organisms at both localities 15 (Loskop Dam) in the upper catchment and 17 (Mamba weir, Kruger National Park) in the lower catchment are at present being chronically exposed to elevated levels of the investigated metals, compared to both the control site and results in literature. The impact of the highly polluted Selati River in the lower catchment was evident in the difference between metal concentrations detected in fish at locality 17, and at locality 19 (Phalaborwa Barrage) upstream of the Olifants-Selati confluence. The present study clearly indicates that the Olifants River is subjected to various sources of pollution which could be detrimental to the health of this aquatic ecosystem. It is recommended (see Chapter 9) that a multi-disciplinary approach including a biomonitoring programme, should be followed to ensure a sustainable freshwater ecosystem.

The management of South Africa's heritage resources is still lacking a great deal and many resources are being destroyed by ignorance, development and plain greed. Although South African legislation is adequate in theory, commitment on the side of the government in terms of staff, skills and resources to implement it, is not forthcoming. On the global platform however, we have much to learn as heritage management is regarded as a priority in most of the first world countries. Four steps to reach this goal, is summarizing it effectively: the identification, understanding how it was created and used, selecting the appropriate conservation techniques, and stakeholder involvement. The focus of this study is on the diverse heritage resources of the Blyde Canyon Nature Reserve in Mpumalanga, the largest green canyon in the world. This area has a rich, irreplaceable and unexplored cultural heritage. The tangible and intangible values were classified in a typological framework, including archaeology (stone age and iron age), rock art, historical industrial features, sacred sites, monuments, burial sites, graves and historic tourist features. Authorities are in the process of changing the status of the Nature Reserve to that of a National Park and it is also envisioned to nominate this unique natural and cultural landscape for World Heritage status. The database and overall objectives of this study has been identified to highlight the relevance and importance of the cultural heritage resources and to ensure its future protection and management. This area is already a popular tourist destination which is currently under-utilised, and by contributing to the protection and responsible development of the heritage resources, and by having the correct management principles set in place, the visitor experience will not only be enhanced but the surrounding towns and communities will benefit extensively. Copyright / Dissertation (MHCS)--University of Pretoria, 2010. / Historical and Heritage Studies / unrestricted

Thesis (M. Dev.) -- University of Limpopo, 2016 / This study aims to give insights into the nature and extent of professional teacher development by the department of education through class visits, and subsequently its impact on professional teacher development in the white hazy circuit in Mpumalanga province. This study further examines measures and recommendations to be put in place which can help to improve the programme (class visits), also the effectiveness of this programme in professional teacher development. The study further highlights key issues regarding class visits with regard to professional teacher development. The study further highlights strategies that can be put in place to enhancing class visits as a programme for professional teacher development. The study further highlights key recommendations to be put in place to enhance class visits as professional teacher development programme. KEY WORDS Professional Teacher Development; Development Appraisal System; Integrated Quality Management Systems; development support group; Continuing Professional Teacher Development.

Please read the abstract in the section 00front of this document / Dissertation (MA (Development Communication))--University of Pretoria, 2006. / Information Science / unrestricted

This study investigated the state of leadership mentoring and succession planning in the Charismatic Churches in Bushbuckridge. In order to gain a full understanding of the Charismatic Movement which emerged in Bushbuckridge only four decades ago, it was necessary to trace its origin from the Azusa Street Revival which gave birth to Pentecostalism almost a century ago. The Charismatic Movement emerged as a distinct movement only five decades later following the birth of the Pentecostal Movement. The reviewed literature revealed that the Charismatic Movement is a child of the marriage between the Pentecostal Movement and traditional denominations. The main watermark distinction between the theologies of the two movements is based on the fact that the Pentecostal Movement emphasises speaking in tongues while the Charismatic Movement places emphasis on the manifestation of the gifts of the Holy Spirit. By virtue of their one-man-founder, Charismatic Churches are likely to experience the same leadership mentoring and succession problems that are common to family businesses. An assessment of leadership mentoring and succession practices in the business world proved that the concept has been researched, practiced, and perfected more in the secular world than in the world of religious and biblical studies. Although this study uncovered some exceptionally successful mentoring relationships in both the New and the Old Testaments, they do not provide details of what those who were involved in the mentoring relationships did for them to be successful. It is in the secular world that mentoring relationships, stages and models have been researched and practiced. It is when mentors and protégés are pared correctly and their relationships managed properly throughout all the mentoring stages that organisations can be assured of smooth leadership transitions. A triangulation of the qualitative and the quantitative methods was used to collect data in this study. On the qualitative side of the research, data was collected by means of interviews and focus group studies. Interviews with the apostolic fathers of the Charismatic Movement in South Africa raised a concern over the lack of leadership mentoring and succession programmes, especially amongst the younger generation of churches. The concern was confirmed in all the focus group studies which were conducted in Bushbuckridge. The data which was collected from the qualitative side of the study provided a basis for the content of the questionnaire the researcher drew up for the quantitative side of the study. This side of the study contradicted the views of the participants in interviews and focus groups in that it reported that many Charismatic Churches in Bushbuckridge do in fact have leadership mentoring and succession programmes (85% and 72% respectively). The quantitative side of the study further revealed that education levels and gender have a statistically significant effect in leadership mentoring, while location does not. The researcher suggests that the contradiction in the outcomes of the two research methods deserve a follow-up study. This study presents to Charismatic Church leaders in Bushbuckridge lessons drawn from the leadership mentoring and succession practices of both the biblical and secular worlds. This study would be incomplete without the bringing to the fore of an illustrative text in the 2 Timothy 2:1-3. Generally speaking, adherents in the Charismatic Churches read and interpret the bible as authoritative for faith and life. The participants‘ call for the introduction or/and enhancement of leadership mentoring and succession is therefore harnessed by the biblical message in 2 Timothy 2:1-3. Such model of leadership could serve as an important and significant form of discourse in Africa in the context of leadership. / Thesis (PhD)--University of Pretoria, 2013. / gm2014 / New Testament Studies / unrestricted

Oliver Tambo institute of Governance and Policy Studies / MPM

The researcher attempts to establish how CCTV footage can be used as a surveillance technique, for evidence gained in that way to be admissible in court. To conduct effective investigation, it is important for investigators to be familiar with the application of CCTV footage, its purpose, its benefits and shortfalls. To achieve the goals and objectives of the practice of CCTV footage, investigators must know how to utilise the value of its evidence, and how to use it to identify suspects. The clarification and application to the crime situation, the identification of the perpetrator or suspect of a criminal act is mostly impossible, if CCTV footage is not available to assist in identification and tracing of suspects. / Police Practice / M. Tech. (Forensic Investigation)